Restriction map for
pFN21AE1345
(Circular) (Six-base) MAPSORT of: pFN21AE1345.seq Check: 7370 from: 1 to: 5497
pFN21AE1345 5497 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2404 2404
Size: 5497
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3307 278
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2840 1937
Size: 903 4594
Acc65I G'GTAC_C
Cuts at: 2818 2818
Size: 5497
AclI AA'CG_TT
Cuts at: 1563 3624 3997 1563
Size: 2061 373 3063
Fragments arranged by size:
3063 2061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2318 2441 2585 3560 4944 5212 1632
Size: 686 123 144 975 1384 268 1917
Fragments arranged by size:
1917 1384 975 686 268 144 123
AflII C'TTAA_G
Cuts at: 820 1017 2117 820
Size: 197 1100 4200
Fragments arranged by size:
4200 1100 197
AflIII A'CryG_T
Cuts at: 1203 4396 5373 1203
Size: 3193 977 1327
Fragments arranged by size:
3193 1327 977
AgeI A'CCGG_T
Cuts at: 4328 4328
Size: 5497
AhdI GACnn_n'nnGTC
Cuts at: 2405 4226 2405
Size: 1821 3676
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5497
AlwNI CAG_nnn'CTG
Cuts at: 2275 2284 2419 2449 3240 4812 2275
Size: 9 135 30 791 1572 2960
Fragments arranged by size:
2960 1572 791 135 30 9
ApaLI G'TGCA_C
Cuts at: 3554 4710 3554
Size: 1156 4341
ApoI r'AATT_y
Cuts at: 1493 2806 3032 3184 5293 1493
Size: 1313 226 152 2109 1697
Fragments arranged by size:
2109 1697 1313 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4051 160
Size: 872 3019 1606
Fragments arranged by size:
3019 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 2822 1870
Size: 73 112 767 4545
Fragments arranged by size:
4545 767 112 73
BamHI G'GATC_C
Cuts at: 2827 3194 2827
Size: 367 5130
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2818 4273 618
Size: 325 217 627 1031 1455 1842
Fragments arranged by size:
1842 1455 1031 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2816 1391
Size: 1425 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3688 3722 1903
Size: 34 1751 34 3678
Fragments arranged by size:
3678 1751 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3389 4599 3389
Size: 1210 4287
BclI T'GATC_A
Cuts at: 1548 2586 1548
Size: 1038 4459
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4108 136
Size: 107 122 71 631 605 2436 1525
Fragments arranged by size:
2436 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5492 5492
Size: 5497
BlpI GC'TnA_GC
Cuts at: 2486 2783 2914 2486
Size: 297 131 5069
Fragments arranged by size:
5069 297 131
Bme1580I G_kGCm'C
Cuts at: 1790 3558 4714 1790
Size: 1768 1156 2573
Fragments arranged by size:
2573 1768 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2324 2459 3228
Size: 416 53 83 399 24 907 135 769
Cuts at: 3228 4186 442
Size: 958 1753
Fragments arranged by size:
1753 958 907 769 416 399 135 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5497
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 5497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2623 4157 1239
Size: 1067 317 1534 2579
Fragments arranged by size:
2579 1534 1067 317
Bpu10I CC'TnA_GC
Cuts at: 2543 2543
Size: 5497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2585 3622 4487 4785 2585
Size: 1037 865 298 3297
Fragments arranged by size:
3297 1037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2166 4160 882
Size: 617 667 1994 2219
Fragments arranged by size:
2219 1994 667 617
BsaAI yAC'GTr
Cuts at: 493 1204 3288 493
Size: 711 2084 2702
Fragments arranged by size:
2702 2084 711
BsaBI GATnn'nnATC
Cuts at: 2186 5491 2186
Size: 3305 2192
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3304 3686 4321 275
Size: 53 83 186 936 1771 382 635 1451
Fragments arranged by size:
1771 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3930 4328 4602 4749 5160 3930
Size: 398 274 147 411 4267
Fragments arranged by size:
4267 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2378 2408 1178
Size: 30 165 30 975 30 4267
Fragments arranged by size:
4267 975 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2402 1186
Size: 1021 195 4281
Fragments arranged by size:
4281 1021 195
BseYI C'CCAG_C
Cuts at: 4700 5196 4700
Size: 496 5001
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4924
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2947 3708 4736 1540
Size: 275 186 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2487 3558 3643 4714 2487
Size: 1071 85 1071 3270
Fragments arranged by size:
3270 1071 1071 85
BsmI GAATG_Cn'
Cuts at: 3008 3101 3008
Size: 93 5404
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2487 2816 3558 3643 4714 1391
Size: 399 697 329 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 697 399 329 85
BspEI T'CCGG_A
Cuts at: 5160 5160
Size: 5497
BspHI T'CATG_A
Cuts at: 3386 5116 3386
Size: 1730 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2184 2283 2404 2852 844
Size: 1340 99 121 448 3489
Fragments arranged by size:
3489 1340 448 121 99
BsrBI CCG'CTC
Cuts at: 1394 1823 3384 1394
Size: 429 1561 3507
Fragments arranged by size:
3507 1561 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3992 4166 65
Size: 1183 2744 174 1396
Fragments arranged by size:
2744 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4141 4328 1700
Size: 110 2331 187 2869
Fragments arranged by size:
2869 2331 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5497
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5497
BssSI C'ACGA_G
Cuts at: 3557 4569 3557
Size: 1012 4485
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5497
BstBI TT'CG_AA
Cuts at: 4301 4301
Size: 5497
BstEII G'GTnAC_C
Cuts at: 1184 2335 1184
Size: 1151 4346
BstYI r'GATC_y
Cuts at: 1972 2827 3194 3579 3596 5048 5134 5146 5492
Size: 855 367 385 17 1452 86 12 346
Cuts at: 5492 1972
Size: 1977
Fragments arranged by size:
1977 1452 855 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2739 513
Size: 552 939 735 3271
Fragments arranged by size:
3271 939 735 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4758
Fragments arranged by size:
4758 683 56
BtsI GCAGTG_nn'
Cuts at: 3013 3807 3827 3013
Size: 794 20 4683
Fragments arranged by size:
4683 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5462
DraI TTT'AAA
Cuts at: 2801 2801
Size: 5497
DraIII CAC_nnn'GTG
Cuts at: 2496 2496
Size: 5497
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4504 809
Size: 507 3188 1802
Fragments arranged by size:
3188 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2742 2944 3833
Size: 54 1425 50 275 18 912 202 889
Cuts at: 3833 5462 8
Size: 1629 43
Fragments arranged by size:
1629 1425 912 889 275 202 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2944 1537
Size: 275 1132 4090
Fragments arranged by size:
4090 1132 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3427 1353
Size: 2074 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4084 4458 4604 387
Size: 1064 138 2495 374 146 1280
Fragments arranged by size:
2495 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2318 2441 2585 2623 3560 4157
Size: 393 674 12 123 144 38 937 597
Cuts at: 4157 4944 5212 1239
Size: 787 268 1524
Fragments arranged by size:
1524 937 787 674 597 393 268 144
123 38 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2533 2533
Size: 5497
EcoRI G'AATT_C
Cuts at: 2806 3184 2806
Size: 378 5119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2748 2748
Size: 5497
FspI TGC'GCA
Cuts at: 4003 4003
Size: 5497
HaeII r_GCGC'y
Cuts at: 1678 2543 4644 1678
Size: 865 2101 2531
Fragments arranged by size:
2531 2101 865
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 2391 2423 4138
Size: 32 135 32 605 32 182 32 1715
Cuts at: 4138 4170 4212 4244 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1715 605 182 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2841 3087 669
Size: 988 281 903 246 3079
Fragments arranged by size:
3079 988 903 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5497
HpaI GTT'AAC
Cuts at: 3087 3087
Size: 5497
KpnI G_GTAC'C
Cuts at: 2822 2822
Size: 5497
MfeI C'AATT_G
Cuts at: 3096 3096
Size: 5497
MluI A'CGCG_T
Cuts at: 5373 5373
Size: 5497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4611 4795 1305
Size: 3306 184 2007
Fragments arranged by size:
3306 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2744 10
Size: 54 1425 343 912 2763
Fragments arranged by size:
2763 1425 912 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3455 3814 3973 518
Size: 648 2289 359 159 2042
Fragments arranged by size:
2289 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2913 3590 4738 4983 1606
Size: 1307 677 1148 245 2120
Fragments arranged by size:
2120 1307 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2739 513
Size: 552 939 735 3271
Fragments arranged by size:
3271 939 735 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5497
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5497
NotI GC'GGCC_GC
Cuts at: 2944 2944
Size: 5497
NspI r_CATG'y
Cuts at: 1132 1229 2855 4400 1132
Size: 97 1626 1545 2229
Fragments arranged by size:
2229 1626 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2650 1348
Size: 1302 4195
PciI A'CATG_T
Cuts at: 4396 4396
Size: 5497
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2449 1108
Size: 1341 4156
PmeI GTTT'AAAC
Cuts at: 2801 2801
Size: 5497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2618 2650 3566 3598 5079 5111 2618
Size: 32 916 32 1481 32 3004
Fragments arranged by size:
3004 1481 916 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5497
PsiI TTA'TAA
Cuts at: 3067 3067
Size: 5497
PstI C_TGCA'G
Cuts at: 830 2104 2280 2537 2849 830
Size: 1274 176 257 312 3478
Fragments arranged by size:
3478 1274 312 257 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5497
SalI G'TCGA_C
Cuts at: 1936 2839 1936
Size: 903 4594
SbfI CC_TGCA'GG
Cuts at: 2280 2849 2280
Size: 569 4928
ScaI AGT'ACT
Cuts at: 3745 5457 3745
Size: 1712 3785
SfcI C'TryA_G
Cuts at: 826 1045 2100 2276 2533 2845 3980 4661 4852
Size: 219 1055 176 257 312 1135 681 191
Cuts at: 4852 826
Size: 1471
Fragments arranged by size:
1471 1135 1055 681 312 257 219 191
176
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5497
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5497
SmaI CCC'GGG
Cuts at: 1872 2824 1872
Size: 952 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2117 2600 3601 4502 4764 820
Size: 197 926 174 483 1001 901 262 1553
Fragments arranged by size:
1553 1001 926 901 483 262 197 174
SnaBI TAC'GTA
Cuts at: 493 3288 493
Size: 2795 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5497
SphI G_CATG'C
Cuts at: 1132 2855 1132
Size: 1723 3774
SspI AAT'ATT
Cuts at: 5 52 2075 3421 5
Size: 47 2023 1346 2081
Fragments arranged by size:
2081 2023 1346 47
StuI AGG'CCT
Cuts at: 1517 2315 1517
Size: 798 4699
StyI C'CwwG_G
Cuts at: 513 1065 2004 2025 2739 2936 513
Size: 552 939 21 714 197 3074
Fragments arranged by size:
3074 939 714 552 197 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3524 3541 3694 3879 4324 325
Size: 1255 1944 17 153 185 445 1498
Fragments arranged by size:
1944 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3743 5455 96
Size: 274 80 33 51 1443 1766 1712 138
Fragments arranged by size:
1766 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5457 5489 5457
Size: 32 5465
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5497
XbaI T'CTAG_A
Cuts at: 2833 2833
Size: 5497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2647 2647
Size: 5497
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5497
XmaI C'CCGG_G
Cuts at: 1870 2822 1870
Size: 952 4545
XmnI GAAnn'nnTTC
Cuts at: 3626 3626
Size: 5497
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3305 276
Size: 53 83 186 936 1771 2468
Fragments arranged by size:
2468 1771 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI