Restriction map for
pFN21AE1339
(Circular) (Six-base) MAPSORT of: pFN21AE1339.seq Check: 2179 from: 1 to: 6265
pFN21AE1339 6265 bp
With 182 enzymes: SgfI *
December 17, 2009 17:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2533 3110 2533
Size: 577 5688
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2406 3608 1937
Size: 469 1202 4594
Fragments arranged by size:
4594 1202 469
Acc65I G'GTAC_C
Cuts at: 3326 3586 3326
Size: 260 6005
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3330 4328 5712 5980 1632
Size: 1698 998 1384 268 1917
Fragments arranged by size:
1917 1698 1384 998 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 5164 6141 1203
Size: 3961 977 1327
Fragments arranged by size:
3961 1327 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 2278 4994 2278
Size: 2716 3549
AleI CACnn'nnGTG
Cuts at: 1166 2958 1166
Size: 1792 4473
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AlwNI CAG_nnn'CTG
Cuts at: 3116 4008 5580 3116
Size: 892 1572 3801
Fragments arranged by size:
3801 1572 892
ApaI G_GGCC'C
Cuts at: 2936 3415 2936
Size: 479 5786
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 2019 3361 3574 3800 3952 6061 1493
Size: 526 1342 213 226 152 2109 1697
Fragments arranged by size:
2109 1697 1342 526 226 213 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 2578 3020 3590 1870
Size: 73 635 442 570 4545
Fragments arranged by size:
4545 635 570 442 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3310 3343 3310
Size: 33 6232
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2456 2501 2663 2960 3326
Size: 325 217 627 669 45 162 297 366
Cuts at: 3326 3586 5041 618
Size: 260 1455 1842
Fragments arranged by size:
1842 1455 669 627 366 325 297 260
217 162 45
BanII G_rGCy'C
Cuts at: 1391 2040 2830 2936 3020 3146 3172 3415 3584
Size: 649 790 106 84 126 26 243 169
Cuts at: 3584 1391
Size: 4072
Fragments arranged by size:
4072 790 649 243 169 126 106 84
26
BbeI G_GCGC'C
Cuts at: 2460 2460
Size: 6265
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2459 3370 928
Size: 971 560 911 3823
Fragments arranged by size:
3823 971 911 560
BbvCI CC'TCA_GC
Cuts at: 2268 2268
Size: 6265
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2546 4876 136
Size: 107 122 71 631 605 874 2330 1525
Fragments arranged by size:
2330 1525 874 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3444 6260 3444
Size: 2816 3449
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 2506 2936 3415 3476 4326 5482 1790
Size: 716 430 479 61 850 1156 2573
Fragments arranged by size:
2573 1156 850 716 479 430 61
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3447 3996 4954
Size: 416 53 83 399 24 2030 549 958
Cuts at: 4954 442
Size: 1753
Fragments arranged by size:
2030 1753 958 549 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2331 2625 2844 2907 4925 1239
Size: 1016 76 294 219 63 2018 2579
Fragments arranged by size:
2579 2018 1016 294 219 76 63
Bpu10I CC'TnA_GC
Cuts at: 2268 2325 2268
Size: 57 6208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2009 2062 4390 5255 5553 2009
Size: 53 2328 865 298 2721
Fragments arranged by size:
2721 2328 865 298 53
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2042 2714 2748 2768 3189 4928 882
Size: 617 543 672 34 20 421 1739 2219
Fragments arranged by size:
2219 1739 672 617 543 421 34 20
BsaAI yAC'GTr
Cuts at: 493 1204 4056 493
Size: 711 2852 2702
Fragments arranged by size:
2852 2702 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2457 4072 4454 5089
Size: 53 83 186 936 924 1615 382 635
Cuts at: 5089 275
Size: 1451
Fragments arranged by size:
1615 1451 936 924 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4698 5096 5370 5517 5928 4698
Size: 398 274 147 411 5035
Fragments arranged by size:
5035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2827 2857 1178
Size: 30 165 30 1424 30 4586
Fragments arranged by size:
4586 1424 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2131 3551 1186
Size: 945 1420 3900
Fragments arranged by size:
3900 1420 945
BseYI C'CCAG_C
Cuts at: 2183 3391 3510 5468 5964 2183
Size: 1208 119 1958 496 2484
Fragments arranged by size:
2484 1958 1208 496 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2964 3481 3577 1163
Size: 573 1228 517 96 3851
Fragments arranged by size:
3851 1228 573 517 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 2391 2641 4326 4411 5482 2040
Size: 351 250 1685 85 1071 2823
Fragments arranged by size:
2823 1685 1071 351 250 85
BsmI GAATG_Cn'
Cuts at: 3776 3869 3776
Size: 93 6172
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2391 2506 2641 2830 2936 3020
Size: 399 250 351 115 135 189 106 84
Cuts at: 3020 3146 3172 3415 3476 3584 4326 4411 5482
Size: 126 26 243 61 108 742 85 1071
Cuts at: 5482 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 351 250 243 189
135 126 115 108 106 85 84 61
26
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 3520 4154 5884 3520
Size: 634 1730 3901
Fragments arranged by size:
3901 1730 634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2533 3110 3146 3620 844
Size: 1689 577 36 474 3489
Fragments arranged by size:
3489 1689 577 474 36
BsrBI CCG'CTC
Cuts at: 1394 1823 4152 1394
Size: 429 2329 3507
Fragments arranged by size:
3507 2329 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2085 4760 4934 65
Size: 1183 837 2675 174 1396
Fragments arranged by size:
2675 1396 1183 837 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4909 5096 1700
Size: 110 3099 187 2869
Fragments arranged by size:
3099 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3417 96
Size: 3321 2944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 3533 4325 5337 3533
Size: 792 1012 4461
Fragments arranged by size:
4461 1012 792
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2350 2395 794
Size: 1556 45 4664
Fragments arranged by size:
4664 1556 45
BstBI TT'CG_AA
Cuts at: 2091 5069 2091
Size: 2978 3287
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 3212 3444 3595 3962 4347 4364 5816 5902
Size: 1240 232 151 367 385 17 1452 86
Cuts at: 5902 5914 6260 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1240 385 367 346 232 151
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2487 513
Size: 552 1422 4291
Fragments arranged by size:
4291 1422 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2715 3360 487
Size: 683 56 1489 645 3392
Fragments arranged by size:
3392 1489 683 645 56
BtsI GCAGTG_nn'
Cuts at: 2952 2986 3781 4575 4595 2952
Size: 34 795 794 20 4622
Fragments arranged by size:
4622 795 794 34 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2853 2888 529
Size: 35 2289 35 3906
Fragments arranged by size:
3906 2289 35 35
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3184 3712 4601
Size: 54 1425 50 275 18 1354 528 889
Cuts at: 4601 6230 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1354 889 528 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4195 1353
Size: 2842 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4852 5226 5372 387
Size: 1064 138 3263 374 146 1280
Fragments arranged by size:
3263 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2038 2038
Size: 6265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2331 2625 2844 2907 3330 4328
Size: 393 623 76 294 219 63 423 998
Cuts at: 4328 4925 5712 5980 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 998 787 623 597 423 393 294
268 219 76 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 2324
Size: 6265
EcoO109I rG'GnC_Cy
Cuts at: 2230 2573 2582 2627 2672 2816 2933 3113 3158
Size: 343 9 45 45 144 117 180 45
Cuts at: 3158 3411 3412 2230
Size: 253 1 5083
Fragments arranged by size:
5083 343 253 180 144 117 45 45
45 9 1
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 2184 2460 5412 1678
Size: 506 276 2952 2531
Fragments arranged by size:
2952 2531 506 276
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 4906 4938 4980
Size: 32 135 32 692 32 2610 32 42
Cuts at: 4980 5012 1373
Size: 32 2626
Fragments arranged by size:
2626 2610 692 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 669
Size: 988 281 1671 246 3079
Fragments arranged by size:
3079 1671 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KasI G'GCGC_C
Cuts at: 2456 2456
Size: 6265
KpnI G_GTAC'C
Cuts at: 3330 3590 3330
Size: 260 6005
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5379 5563 1305
Size: 4074 184 2007
Fragments arranged by size:
4074 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3186 10
Size: 54 1425 343 1354 3089
Fragments arranged by size:
3089 1425 1354 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2958 4223 4582 4741 518
Size: 648 1326 466 1265 359 159 2042
Fragments arranged by size:
2042 1326 1265 648 466 359 159
MspA1I CmG'CkG
Cuts at: 1606 2177 2401 3681 4358 5506 5751 1606
Size: 571 224 1280 677 1148 245 2120
Fragments arranged by size:
2120 1280 1148 677 571 245 224
NarI GG'CG_CC
Cuts at: 2457 2457
Size: 6265
NcoI C'CATG_G
Cuts at: 513 1065 2487 513
Size: 552 1422 4291
Fragments arranged by size:
4291 1422 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2497 3391 3623 5168 1132
Size: 97 1268 894 232 1545 2229
Fragments arranged by size:
2229 1545 1268 894 232 97
PasI CC'CwG_GG
Cuts at: 1348 2426 2632 2830 2993 3146 1348
Size: 1078 206 198 163 153 4467
Fragments arranged by size:
4467 1078 206 198 163 153
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6265
PfoI T'CCnGG_A
Cuts at: 2232 2821 2884 3028 2232
Size: 589 63 144 5469
Fragments arranged by size:
5469 589 144 63
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2230 2582 2627 2672 3158 2230
Size: 352 45 45 486 5337
Fragments arranged by size:
5337 486 352 45 45
PshAI GACnn'nnGTC
Cuts at: 1613 2760 1613
Size: 1147 5118
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PspOMI G'GGCC_C
Cuts at: 2932 3411 2932
Size: 479 5786
PstI C_TGCA'G
Cuts at: 830 2217 2298 2355 2400 2922 3121 3530 3617
Size: 1387 81 57 45 522 199 409 87
Cuts at: 3617 830
Size: 3478
Fragments arranged by size:
3478 1387 522 409 199 87 81 57
45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
SacI G_AGCT'C
Cuts at: 2040 2040
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SanDI GG'GwC_CC
Cuts at: 2582 2582
Size: 6265
SbfI CC_TGCA'GG
Cuts at: 2355 3121 3617 2355
Size: 766 496 5003
Fragments arranged by size:
5003 766 496
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SexAI A'CCwGG_T
Cuts at: 2866 2866
Size: 6265
SfcI C'TryA_G
Cuts at: 826 1045 2213 2294 2351 2396 2918 3117 3319
Size: 219 1168 81 57 45 522 199 202
Cuts at: 3319 3526 3613 4748 5429 5620 826
Size: 207 87 1135 681 191 1471
Fragments arranged by size:
1471 1168 1135 681 522 219 207 202
199 191 87 81 57 45
SfoI GGC'GCC
Cuts at: 2458 2458
Size: 6265
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3022 3592 1872
Size: 1150 570 4545
Fragments arranged by size:
4545 1150 570
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2024 2041 4369 5270 5532 820
Size: 197 926 81 17 2328 901 262 1553
Fragments arranged by size:
2328 1553 926 901 262 197 81 17
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 2497 3391 3623 1132
Size: 1365 894 232 3774
Fragments arranged by size:
3774 1365 894 232
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 3042 1517
Size: 1525 4740
StyI C'CwwG_G
Cuts at: 513 1065 2487 2541 2587 2623 2677 2964 3162
Size: 552 1422 54 46 36 54 287 198
Cuts at: 3162 3704 513
Size: 542 3074
Fragments arranged by size:
3074 1422 552 542 287 198 54 54
46 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2970 4292 4309 4462 4647 5092 325
Size: 1255 1390 1322 17 153 185 445 1498
Fragments arranged by size:
1498 1390 1322 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3417 4511 6223
Size: 274 80 33 51 1443 1440 1094 1712
Cuts at: 6223 96
Size: 138
Fragments arranged by size:
1712 1443 1440 1094 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2827 2859 6225 6257 2827
Size: 32 3366 32 2835
Fragments arranged by size:
3366 2835 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2548 2548
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 3020 3590 1870
Size: 1150 570 4545
Fragments arranged by size:
4545 1150 570
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacII SapI SfiI SrfI SwaI