Restriction map for
pFN21AE1335
(Circular) (Six-base) MAPSORT of: pFN21AE1335.seq Check: 5418 from: 1 to: 5401
pFN21AE1335 5401 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2445 2445
Size: 5401
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3211 278
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 1937
Size: 807 4594
Acc65I G'GTAC_C
Cuts at: 2722 2722
Size: 5401
AclI AA'CG_TT
Cuts at: 1563 3528 3901 1563
Size: 1965 373 3063
Fragments arranged by size:
3063 1965 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2480 2498 3464 4848 5116 1632
Size: 848 18 966 1384 268 1917
Fragments arranged by size:
1917 1384 966 848 268 18
AflII C'TTAA_G
Cuts at: 820 1017 2510 820
Size: 197 1493 3711
Fragments arranged by size:
3711 1493 197
AflIII A'CryG_T
Cuts at: 1203 4300 5277 1203
Size: 3097 977 1327
Fragments arranged by size:
3097 1327 977
AgeI A'CCGG_T
Cuts at: 4232 4232
Size: 5401
AhdI GACnn_n'nnGTC
Cuts at: 4130 4130
Size: 5401
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5401
AlwNI CAG_nnn'CTG
Cuts at: 2028 2266 2356 2683 3144 4716 2028
Size: 238 90 327 461 1572 2713
Fragments arranged by size:
2713 1572 461 327 238 90
ApaI G_GGCC'C
Cuts at: 2122 2385 2690 2122
Size: 263 305 4833
Fragments arranged by size:
4833 305 263
ApaLI G'TGCA_C
Cuts at: 3458 4614 3458
Size: 1156 4245
ApoI r'AATT_y
Cuts at: 1493 2710 2936 3088 5197 1493
Size: 1217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1217 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3955 160
Size: 872 2923 1606
Fragments arranged by size:
2923 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
AvaI C'yCGr_G
Cuts at: 1870 1943 2726 1870
Size: 73 783 4545
Fragments arranged by size:
4545 783 73
BamHI G'GATC_C
Cuts at: 2731 3098 2731
Size: 367 5034
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2109 2722 4177 618
Size: 325 217 627 322 613 1455 1842
Fragments arranged by size:
1842 1455 627 613 325 322 217
BanII G_rGCy'C
Cuts at: 1391 2122 2149 2371 2385 2690 2720 1391
Size: 731 27 222 14 305 30 4072
Fragments arranged by size:
4072 731 305 222 30 27 14
BbeI G_GCGC'C
Cuts at: 2113 2113
Size: 5401
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2646 928
Size: 971 747 3683
Fragments arranged by size:
3683 971 747
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3592 3626 1903
Size: 34 50 34 1571 34 3678
Fragments arranged by size:
3678 1571 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3293 4503 3293
Size: 1210 4191
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2380 4012 136
Size: 107 122 71 631 605 708 1632 1525
Fragments arranged by size:
1632 1525 708 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5396 5396
Size: 5401
BlpI GC'TnA_GC
Cuts at: 2818 2818
Size: 5401
Bme1580I G_kGCm'C
Cuts at: 1790 2122 2385 2690 3462 4618 1790
Size: 332 263 305 772 1156 2573
Fragments arranged by size:
2573 1156 772 332 305 263
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5401
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2684 3132 4090
Size: 416 53 83 399 24 1267 448 958
Cuts at: 4090 442
Size: 1753
Fragments arranged by size:
1753 1267 958 448 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2426 2663 4061 1239
Size: 1055 132 237 1398 2579
Fragments arranged by size:
2579 1398 1055 237 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3526 4391 4689 3526
Size: 865 298 4238
Fragments arranged by size:
4238 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2519 4064 882
Size: 617 1020 1545 2219
Fragments arranged by size:
2219 1545 1020 617
BsaAI yAC'GTr
Cuts at: 493 1204 3192 493
Size: 711 1988 2702
Fragments arranged by size:
2702 1988 711
BsaBI GATnn'nnATC
Cuts at: 5395 5395
Size: 5401
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2110 3208 3590 4225
Size: 53 83 186 936 577 1098 382 635
Cuts at: 4225 275
Size: 1451
Fragments arranged by size:
1451 1098 936 635 577 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3834 4232 4506 4653 5064 3834
Size: 398 274 147 411 4171
Fragments arranged by size:
4171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 2117 2147 2602
Size: 30 165 30 657 30 27 30 455
Cuts at: 2602 2632 2636 2666 1178
Size: 30 4 30 3913
Fragments arranged by size:
3913 657 455 165 30 30 30 30
30 30 27 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2029 2174 1186
Size: 843 145 4413
Fragments arranged by size:
4413 843 145
BseYI C'CCAG_C
Cuts at: 2101 2662 4604 5100 2101
Size: 561 1942 496 2402
Fragments arranged by size:
2402 1942 561 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2155 1163
Size: 573 419 4409
Fragments arranged by size:
4409 573 419
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2851 3612 4640 1540
Size: 275 186 850 761 1028 2301
Fragments arranged by size:
2301 1028 850 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3462 3547 4618 3462
Size: 85 1071 4245
Fragments arranged by size:
4245 1071 85
BsmI GAATG_Cn'
Cuts at: 2398 2452 2912 3005 2398
Size: 54 460 93 4794
Fragments arranged by size:
4794 460 93 54
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 2149 2371 2385 2690 2720 3462
Size: 399 332 27 222 14 305 30 742
Cuts at: 3462 3547 4618 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 332 305 222 85
30 27 14
BspEI T'CCGG_A
Cuts at: 5064 5064
Size: 5401
BspHI T'CATG_A
Cuts at: 3290 5020 3290
Size: 1730 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 2289 2445 2756 844
Size: 1430 15 156 311 3489
Fragments arranged by size:
3489 1430 311 156 15
BsrBI CCG'CTC
Cuts at: 1394 1823 3288 1394
Size: 429 1465 3507
Fragments arranged by size:
3507 1465 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3896 4070 65
Size: 1183 2648 174 1396
Fragments arranged by size:
2648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2106 2232 4045 4232 1700
Size: 110 296 126 1813 187 2869
Fragments arranged by size:
2869 1813 296 187 126 110
BsrGI T'GTAC_A
Cuts at: 96 2151 96
Size: 2055 3346
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5401
BssSI C'ACGA_G
Cuts at: 3461 4473 3461
Size: 1012 4389
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2356 794
Size: 1562 3839
BstBI TT'CG_AA
Cuts at: 4205 4205
Size: 5401
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5401
BstYI r'GATC_y
Cuts at: 1972 2680 2731 3098 3483 3500 4952 5038 5050
Size: 708 51 367 385 17 1452 86 12
Cuts at: 5050 5396 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 708 385 367 346 86 51
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2140 2361 2520 2697 513
Size: 552 1075 221 159 177 3217
Fragments arranged by size:
3217 1075 552 221 177 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4662
Fragments arranged by size:
4662 683 56
BtsI GCAGTG_nn'
Cuts at: 2917 3711 3731 2917
Size: 794 20 4587
Fragments arranged by size:
4587 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5366
DraI TTT'AAA
Cuts at: 2705 2705
Size: 5401
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2540 4408 809
Size: 507 1224 1868 1802
Fragments arranged by size:
1868 1802 1224 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2191 2331 2848
Size: 54 1425 50 275 18 361 140 517
Cuts at: 2848 3737 5366 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 517 361 275 140 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2848 1537
Size: 275 1036 4090
Fragments arranged by size:
4090 1036 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3331 1353
Size: 1978 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3988 4362 4508 387
Size: 1064 138 2399 374 146 1280
Fragments arranged by size:
2399 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2426 2480 2498 2663 3464 4061
Size: 393 662 132 54 18 165 801 597
Cuts at: 4061 4848 5116 1239
Size: 787 268 1524
Fragments arranged by size:
1524 801 787 662 597 393 268 165
132 54 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2375 2375
Size: 5401
EcoO109I rG'GnC_Cy
Cuts at: 2118 2119 2381 2647 2118
Size: 1 262 266 4872
Fragments arranged by size:
4872 266 262 1
EcoRI G'AATT_C
Cuts at: 2710 3088 2710
Size: 378 5023
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 5401
FspI TGC'GCA
Cuts at: 2285 2327 3907 2285
Size: 42 1580 3779
Fragments arranged by size:
3779 1580 42
FspAI rTGC'GCAy
Cuts at: 2327 2327
Size: 5401
HaeII r_GCGC'y
Cuts at: 1678 2113 2357 4548 1678
Size: 435 244 2191 2531
Fragments arranged by size:
2531 2191 435 244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2636 2668 4042
Size: 32 135 32 488 32 544 32 1374
Cuts at: 4042 4074 4116 4148 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1374 544 488 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2745 2991 669
Size: 988 281 807 246 3079
Fragments arranged by size:
3079 988 807 281 246
HindIII A'AGCT_T
Cuts at: 748 2291 748
Size: 1543 3858
HpaI GTT'AAC
Cuts at: 2991 2991
Size: 5401
KasI G'GCGC_C
Cuts at: 2109 2109
Size: 5401
KpnI G_GTAC'C
Cuts at: 2726 2726
Size: 5401
MfeI C'AATT_G
Cuts at: 3000 3000
Size: 5401
MluI A'CGCG_T
Cuts at: 5277 5277
Size: 5401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 4515 4699 1305
Size: 798 2412 184 2007
Fragments arranged by size:
2412 2007 798 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2193 2333 10
Size: 54 1425 343 361 140 3078
Fragments arranged by size:
3078 1425 361 343 140 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 3359 3718 3877 518
Size: 648 1005 1188 359 159 2042
Fragments arranged by size:
2042 1188 1005 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2142 2272 2341 2817 3494 4642 4887 1606
Size: 536 130 69 476 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 536 476 245 130 69
NaeI GCC'GGC
Cuts at: 2108 2234 2108
Size: 126 5275
NarI GG'CG_CC
Cuts at: 2110 2110
Size: 5401
NcoI C'CATG_G
Cuts at: 513 1065 2361 2520 2697 513
Size: 552 1296 159 177 3217
Fragments arranged by size:
3217 1296 552 177 159
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5401
NgoMIV G'CCGG_C
Cuts at: 2106 2232 2106
Size: 126 5275
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5401
NotI GC'GGCC_GC
Cuts at: 2848 2848
Size: 5401
NspI r_CATG'y
Cuts at: 1132 1229 2759 4304 1132
Size: 97 1530 1545 2229
Fragments arranged by size:
2229 1545 1530 97
PasI CC'CwG_GG
Cuts at: 1348 2114 2377 1348
Size: 766 263 4372
Fragments arranged by size:
4372 766 263
PciI A'CATG_T
Cuts at: 4300 4300
Size: 5401
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2535 1108
Size: 1427 3974
PfoI T'CCnGG_A
Cuts at: 2490 2490
Size: 5401
PmeI GTTT'AAAC
Cuts at: 2705 2705
Size: 5401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 4983 5015 3470
Size: 32 1481 32 3856
Fragments arranged by size:
3856 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2647 2647
Size: 5401
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5401
PsiI TTA'TAA
Cuts at: 2971 2971
Size: 5401
PspOMI G'GGCC_C
Cuts at: 2118 2381 2686 2118
Size: 263 305 4833
Fragments arranged by size:
4833 305 263
PstI C_TGCA'G
Cuts at: 830 2271 2753 830
Size: 1441 482 3478
Fragments arranged by size:
3478 1441 482
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5401
PvuII CAG'CTG
Cuts at: 2272 2272
Size: 5401
SacII CC_GC'GG
Cuts at: 2143 2143
Size: 5401
SalI G'TCGA_C
Cuts at: 1936 2743 1936
Size: 807 4594
SbfI CC_TGCA'GG
Cuts at: 2753 2753
Size: 5401
ScaI AGT'ACT
Cuts at: 3649 5361 3649
Size: 1712 3689
SfcI C'TryA_G
Cuts at: 826 1045 2267 2749 3884 4565 4756 826
Size: 219 1222 482 1135 681 191 1471
Fragments arranged by size:
1471 1222 1135 681 482 219 191
SfoI GGC'GCC
Cuts at: 2111 2111
Size: 5401
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5401
SmaI CCC'GGG
Cuts at: 1872 2728 1872
Size: 856 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2510 3505 4406 4668 820
Size: 197 926 567 995 901 262 1553
Fragments arranged by size:
1553 995 926 901 567 262 197
SnaBI TAC'GTA
Cuts at: 493 3192 493
Size: 2699 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5401
SphI G_CATG'C
Cuts at: 1132 2759 1132
Size: 1627 3774
SspI AAT'ATT
Cuts at: 5 52 3325 5
Size: 47 3273 2081
Fragments arranged by size:
3273 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5401
StyI C'CwwG_G
Cuts at: 513 1065 2361 2520 2595 2697 2840 513
Size: 552 1296 159 75 102 143 3074
Fragments arranged by size:
3074 1296 552 159 143 102 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2654 3428 3445 3598 3783 4228 325
Size: 1255 1074 774 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1074 774 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 3647 5359
Size: 274 80 33 51 1443 174 1496 1712
Cuts at: 5359 96
Size: 138
Fragments arranged by size:
1712 1496 1443 274 174 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2117 2149 2602 2634 5361 5393 2117
Size: 32 453 32 2727 32 2125
Fragments arranged by size:
2727 2125 453 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5401
XbaI T'CTAG_A
Cuts at: 2737 2737
Size: 5401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2680 2680
Size: 5401
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5401
XmaI C'CCGG_G
Cuts at: 1870 2726 1870
Size: 856 4545
XmnI GAAnn'nnTTC
Cuts at: 3530 3530
Size: 5401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3209 276
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI