Restriction map for
pFN21AE1332
(Circular) (Six-base) MAPSORT of: pFN21AE1332.seq Check: 624 from: 1 to: 5374
pFN21AE1332 5374 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3184 278
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2717 1937
Size: 780 4594
Acc65I G'GTAC_C
Cuts at: 2695 2695
Size: 5374
AclI AA'CG_TT
Cuts at: 1563 3501 3874 1563
Size: 1938 373 3063
Fragments arranged by size:
3063 1938 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2173 2234 2403 2606 3437 4821 5089 1632
Size: 541 61 169 203 831 1384 268 1917
Fragments arranged by size:
1917 1384 831 541 268 203 169 61
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5177
AflIII A'CryG_T
Cuts at: 1203 4273 5250 1203
Size: 3070 977 1327
Fragments arranged by size:
3070 1327 977
AgeI A'CCGG_T
Cuts at: 4205 4205
Size: 5374
AhdI GACnn_n'nnGTC
Cuts at: 4103 4103
Size: 5374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5374
AlwNI CAG_nnn'CTG
Cuts at: 2138 3117 4689 2138
Size: 979 1572 2823
Fragments arranged by size:
2823 1572 979
ApaLI G'TGCA_C
Cuts at: 3431 4587 3431
Size: 1156 4218
ApoI r'AATT_y
Cuts at: 1493 2266 2479 2612 2683 2909 3061 5170 1493
Size: 773 213 133 71 226 152 2109 1697
Fragments arranged by size:
2109 1697 773 226 213 152 133 71
AseI AT'TA_AT
Cuts at: 160 1032 3928 160
Size: 872 2896 1606
Fragments arranged by size:
2896 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2699 1870
Size: 73 90 666 4545
Fragments arranged by size:
4545 666 90 73
BamHI G'GATC_C
Cuts at: 2704 3071 2704
Size: 367 5007
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2128 2561 2695 4150 618
Size: 325 217 627 341 433 134 1455 1842
Fragments arranged by size:
1842 1455 627 433 341 325 217 134
BanII G_rGCy'C
Cuts at: 1391 2126 2693 1391
Size: 735 567 4072
Fragments arranged by size:
4072 735 567
BbeI G_GCGC'C
Cuts at: 2565 2565
Size: 5374
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2391 928
Size: 971 492 3911
Fragments arranged by size:
3911 971 492
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3565 3599 1903
Size: 34 50 34 1544 34 3678
Fragments arranged by size:
3678 1544 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3266 4476 3266
Size: 1210 4164
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5374
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2423 3985 136
Size: 107 122 71 631 605 751 1562 1525
Fragments arranged by size:
1562 1525 751 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5369 5369
Size: 5374
BlpI GC'TnA_GC
Cuts at: 2791 2791
Size: 5374
Bme1580I G_kGCm'C
Cuts at: 1790 3435 4591 1790
Size: 1645 1156 2573
Fragments arranged by size:
2573 1645 1156
BmgBI CAC'GTC
Cuts at: 1318 2095 1318
Size: 777 4597
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2330 3105 4063
Size: 416 53 83 399 24 913 775 958
Cuts at: 4063 442
Size: 1753
Fragments arranged by size:
1753 958 913 775 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5374
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4034 1239
Size: 2795 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1997 3499 4364 4662 1997
Size: 1502 865 298 2709
Fragments arranged by size:
2709 1502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4037 882
Size: 617 2538 2219
Fragments arranged by size:
2538 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3165 493
Size: 711 1961 2702
Fragments arranged by size:
2702 1961 711
BsaBI GATnn'nnATC
Cuts at: 2024 5368 2024
Size: 3344 2030
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2562 3181 3563 4198
Size: 53 83 186 936 1029 619 382 635
Cuts at: 4198 275
Size: 1451
Fragments arranged by size:
1451 1029 936 635 619 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2548 3807 4205 4479 4626 5037 2548
Size: 1259 398 274 147 411 2885
Fragments arranged by size:
2885 1259 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5149
Fragments arranged by size:
5149 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 1186
Size: 940 4434
BseYI C'CCAG_C
Cuts at: 2062 2098 2175 4577 5073 2062
Size: 36 77 2402 496 2363
Fragments arranged by size:
2402 2363 496 77 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4801
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2824 3585 4613 1540
Size: 275 186 823 761 1028 2301
Fragments arranged by size:
2301 1028 823 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3435 3520 4591 3435
Size: 85 1071 4218
Fragments arranged by size:
4218 1071 85
BsmI GAATG_Cn'
Cuts at: 2544 2885 2978 2544
Size: 341 93 4940
Fragments arranged by size:
4940 341 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2693 3435 3520 4591 1391
Size: 399 336 567 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 567 399 336 85
BspEI T'CCGG_A
Cuts at: 5037 5037
Size: 5374
BspHI T'CATG_A
Cuts at: 2247 3263 4993 2247
Size: 1016 1730 2628
Fragments arranged by size:
2628 1730 1016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2729 844
Size: 1885 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3261 1394
Size: 429 1438 3507
Fragments arranged by size:
3507 1438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3869 4043 65
Size: 1183 2621 174 1396
Fragments arranged by size:
2621 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2072 4018 4205 1700
Size: 110 262 1946 187 2869
Fragments arranged by size:
2869 1946 262 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5374
BssHII G'CGCG_C
Cuts at: 1925 2145 1925
Size: 220 5154
BssSI C'ACGA_G
Cuts at: 3434 4446 3434
Size: 1012 4362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5374
BstBI TT'CG_AA
Cuts at: 4178 4178
Size: 5374
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5374
BstYI r'GATC_y
Cuts at: 1972 2704 3071 3456 3473 4925 5011 5023 5369
Size: 732 367 385 17 1452 86 12 346
Cuts at: 5369 1972
Size: 1977
Fragments arranged by size:
1977 1452 732 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2439 2557 2652 513
Size: 552 1374 118 95 3235
Fragments arranged by size:
3235 1374 552 118 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4635
Fragments arranged by size:
4635 683 56
BtsI GCAGTG_nn'
Cuts at: 2304 2890 3684 3704 2304
Size: 586 794 20 3974
Fragments arranged by size:
3974 794 586 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5339
DraI TTT'AAA
Cuts at: 2678 2678
Size: 5374
DraIII CAC_nnn'GTG
Cuts at: 2299 2299
Size: 5374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4381 809
Size: 507 3065 1802
Fragments arranged by size:
3065 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 2301 2512
Size: 54 1425 50 275 18 244 227 211
Cuts at: 2512 2821 3710 5339 8
Size: 309 889 1629 43
Fragments arranged by size:
1629 1425 889 309 275 244 227 211
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2821 1537
Size: 275 1009 4090
Fragments arranged by size:
4090 1009 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2103 2202 3304 1353
Size: 750 99 1102 3423
Fragments arranged by size:
3423 1102 750 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3961 4335 4481 387
Size: 1064 138 2372 374 146 1280
Fragments arranged by size:
2372 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2234 2403 2606 3437 4034 4821
Size: 393 541 61 169 203 831 597 787
Cuts at: 4821 5089 1239
Size: 268 1524
Fragments arranged by size:
1524 831 787 597 541 393 268 203
169 61
EcoNI CCTnn'n_nnAGG
Cuts at: 2585 2585
Size: 5374
EcoRI G'AATT_C
Cuts at: 2266 2612 2683 3061 2266
Size: 346 71 378 4579
Fragments arranged by size:
4579 378 346 71
FspI TGC'GCA
Cuts at: 3880 3880
Size: 5374
HaeII r_GCGC'y
Cuts at: 1678 2086 2138 2565 4521 1678
Size: 408 52 427 1956 2531
Fragments arranged by size:
2531 1956 427 408 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 2360 2392 4015
Size: 32 135 32 749 32 7 32 1623
Cuts at: 4015 4047 4089 4121 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1623 749 135 42 32 32 32
32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 2964 669
Size: 988 281 780 246 3079
Fragments arranged by size:
3079 988 780 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5374
HpaI GTT'AAC
Cuts at: 2964 2964
Size: 5374
KasI G'GCGC_C
Cuts at: 2561 2561
Size: 5374
KpnI G_GTAC'C
Cuts at: 2699 2699
Size: 5374
MfeI C'AATT_G
Cuts at: 2973 2973
Size: 5374
MluI A'CGCG_T
Cuts at: 5250 5250
Size: 5374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4488 4672 1305
Size: 3183 184 2007
Fragments arranged by size:
3183 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 2514 10
Size: 54 1425 343 471 211 2870
Fragments arranged by size:
2870 1425 471 343 211 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2520 3332 3691 3850 518
Size: 648 1354 812 359 159 2042
Fragments arranged by size:
2042 1354 812 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2790 3467 4615 4860 1606
Size: 1184 677 1148 245 2120
Fragments arranged by size:
2120 1184 1148 677 245
NaeI GCC'GGC
Cuts at: 2074 2074
Size: 5374
NarI GG'CG_CC
Cuts at: 2562 2562
Size: 5374
NcoI C'CATG_G
Cuts at: 513 1065 2557 513
Size: 552 1492 3330
Fragments arranged by size:
3330 1492 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5374
NgoMIV G'CCGG_C
Cuts at: 2072 2072
Size: 5374
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5374
NotI GC'GGCC_GC
Cuts at: 2821 2821
Size: 5374
NspI r_CATG'y
Cuts at: 1132 1229 2732 4277 1132
Size: 97 1503 1545 2229
Fragments arranged by size:
2229 1545 1503 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5374
PciI A'CATG_T
Cuts at: 4273 4273
Size: 5374
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5374
PmeI GTTT'AAAC
Cuts at: 2678 2678
Size: 5374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4956 4988 3443
Size: 32 1481 32 3829
Fragments arranged by size:
3829 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5374
PsiI TTA'TAA
Cuts at: 2944 2944
Size: 5374
PstI C_TGCA'G
Cuts at: 830 2609 2726 830
Size: 1779 117 3478
Fragments arranged by size:
3478 1779 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5374
SalI G'TCGA_C
Cuts at: 1936 2716 1936
Size: 780 4594
SapI GCTCTTCn'nnn_
Cuts at: 2202 2202
Size: 5374
SbfI CC_TGCA'GG
Cuts at: 2726 2726
Size: 5374
ScaI AGT'ACT
Cuts at: 3622 5334 3622
Size: 1712 3662
SfcI C'TryA_G
Cuts at: 826 1045 2605 2722 3857 4538 4729 826
Size: 219 1560 117 1135 681 191 1471
Fragments arranged by size:
1560 1471 1135 681 219 191 117
SfoI GGC'GCC
Cuts at: 2563 2563
Size: 5374
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5374
SmaI CCC'GGG
Cuts at: 1872 2701 1872
Size: 829 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 3478 4379 4641 820
Size: 197 926 69 1466 901 262 1553
Fragments arranged by size:
1553 1466 926 901 262 197 69
SnaBI TAC'GTA
Cuts at: 493 3165 493
Size: 2672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5374
SphI G_CATG'C
Cuts at: 1132 2732 1132
Size: 1600 3774
SspI AAT'ATT
Cuts at: 5 52 3298 5
Size: 47 3246 2081
Fragments arranged by size:
3246 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5374
StyI C'CwwG_G
Cuts at: 513 1065 2557 2598 2813 513
Size: 552 1492 41 215 3074
Fragments arranged by size:
3074 1492 552 215 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3401 3418 3571 3756 4201 325
Size: 1255 1821 17 153 185 445 1498
Fragments arranged by size:
1821 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 3620 5332
Size: 274 80 33 51 1443 264 1379 1712
Cuts at: 5332 96
Size: 138
Fragments arranged by size:
1712 1443 1379 274 264 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2257 2289 5334 5366 2257
Size: 32 3045 32 2265
Fragments arranged by size:
3045 2265 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5374
XbaI T'CTAG_A
Cuts at: 2710 2710
Size: 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5374
XmaI C'CCGG_G
Cuts at: 1870 2699 1870
Size: 829 4545
XmnI GAAnn'nnTTC
Cuts at: 3503 3503
Size: 5374
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3182 276
Size: 53 83 186 936 1648 2468
Fragments arranged by size:
2468 1648 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoO109I EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI
XcmI