Restriction map for
pFN21AE1326
(Circular) (Six-base) MAPSORT of: pFN21AE1326.seq Check: 8554 from: 1 to: 6427
pFN21AE1326 6427 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2811 4237 278
Size: 53 83 186 936 1275 1426 2468
Fragments arranged by size:
2468 1426 1275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3770 1937
Size: 1833 4594
Acc65I G'GTAC_C
Cuts at: 3462 3748 3462
Size: 286 6141
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4490 5874 6142 1632
Size: 2858 1384 268 1917
Fragments arranged by size:
2858 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 5326 6303 1203
Size: 4123 977 1327
Fragments arranged by size:
4123 1327 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 5156 5156
Size: 6427
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3291 1184
Size: 2107 4320
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2012 2044 2558 2590 2012
Size: 32 514 32 5849
Fragments arranged by size:
5849 514 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2155 2185 2554 3124 3454 4170 5742 2155
Size: 30 369 570 330 716 1572 2840
Fragments arranged by size:
2840 1572 716 570 369 330 30
ApaI G_GGCC'C
Cuts at: 2776 2776
Size: 6427
ApaLI G'TGCA_C
Cuts at: 4484 5640 4484
Size: 1156 5271
ApoI r'AATT_y
Cuts at: 1493 3736 3962 4114 6223 1493
Size: 2243 226 152 2109 1697
Fragments arranged by size:
2243 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 2789 3279 3471 3752 1870
Size: 73 846 490 192 281 4545
Fragments arranged by size:
4545 846 490 281 192 73
AvrII C'CTAG_G
Cuts at: 3022 3022
Size: 6427
BamHI G'GATC_C
Cuts at: 2499 3757 4124 2499
Size: 1258 367 4802
Fragments arranged by size:
4802 1258 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3462 3748 5203 618
Size: 325 217 627 1675 286 1455 1842
Fragments arranged by size:
1842 1675 1455 627 325 286 217
BanII G_rGCy'C
Cuts at: 1391 2098 2250 2546 2673 2776 3746 1391
Size: 707 152 296 127 103 970 4072
Fragments arranged by size:
4072 970 707 296 152 127 103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3532 928
Size: 971 1633 3823
Fragments arranged by size:
3823 1633 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2366 2400 2870 2904 4618 4652 1903
Size: 34 429 34 470 34 1714 34 3678
Fragments arranged by size:
3678 1714 470 429 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4319 5529 4319
Size: 1210 5217
BclI T'GATC_A
Cuts at: 1548 2325 1548
Size: 777 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2135 2754 2897
Size: 107 122 71 631 605 463 619 143
Cuts at: 2897 3284 3578 5038 136
Size: 387 294 1460 1525
Fragments arranged by size:
1525 1460 631 619 605 463 387 294
143 122 107 71
BglII A'GATC_T
Cuts at: 6422 6422
Size: 6427
BlpI GC'TnA_GC
Cuts at: 3442 3716 3844 3442
Size: 274 128 6025
Fragments arranged by size:
6025 274 128
Bme1580I G_kGCm'C
Cuts at: 1790 2162 2776 4488 5644 1790
Size: 372 614 1712 1156 2573
Fragments arranged by size:
2573 1712 1156 614 372
BmgBI CAC'GTC
Cuts at: 1318 2884 1318
Size: 1566 4861
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2505 4158 5116
Size: 416 53 83 399 24 1088 1653 958
Cuts at: 5116 442
Size: 1753
Fragments arranged by size:
1753 1653 1088 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2082 3005 3146 3549 5087 1239
Size: 843 923 141 403 1538 2579
Fragments arranged by size:
2579 1538 923 843 403 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 4552 5417 5715 2300
Size: 2252 865 298 3012
Fragments arranged by size:
3012 2252 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2056 2808 3645 5090 882
Size: 617 557 752 837 1445 2219
Fragments arranged by size:
2219 1445 837 752 617 557
BsaAI yAC'GTr
Cuts at: 493 1204 4218 493
Size: 711 3014 2702
Fragments arranged by size:
3014 2702 711
BsaBI GATnn'nnATC
Cuts at: 3591 6421 3591
Size: 2830 3597
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2736 2808 4234 4616
Size: 53 83 186 936 1203 72 1426 382
Cuts at: 4616 5251 275
Size: 635 1451
Fragments arranged by size:
1451 1426 1203 936 635 382 186 83
72 53
BsaWI w'CCGG_w
Cuts at: 2482 2577 2911 4860 5258 5532 5679 6090 2482
Size: 95 334 1949 398 274 147 411 2819
Fragments arranged by size:
2819 1949 411 398 334 274 147 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 3642 3672 1178
Size: 30 165 30 822 30 1387 30 3933
Fragments arranged by size:
3933 1387 822 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3335 3549 1186
Size: 2149 214 4064
Fragments arranged by size:
4064 2149 214
BseYI C'CCAG_C
Cuts at: 2155 3037 3447 5630 6126 2155
Size: 882 410 2183 496 2456
Fragments arranged by size:
2456 2183 882 496 410
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5854
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4638 5666 1540
Size: 275 186 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4488 4573 5644 4488
Size: 85 1071 5271
Fragments arranged by size:
5271 1071 85
BsmI GAATG_Cn'
Cuts at: 3938 4031 3938
Size: 93 6334
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2162 2250 2546 2673 2776 3746
Size: 399 308 64 88 296 127 103 970
Cuts at: 3746 4488 4573 5644 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 970 742 399 308 296 127
103 88 85 64
BspEI T'CCGG_A
Cuts at: 2577 2911 6090 2577
Size: 334 3179 2914
Fragments arranged by size:
3179 2914 334
BspHI T'CATG_A
Cuts at: 3705 4316 6046 3705
Size: 611 1730 4086
Fragments arranged by size:
4086 1730 611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2420 2826 3782 844
Size: 1576 406 956 3489
Fragments arranged by size:
3489 1576 956 406
BsrBI CCG'CTC
Cuts at: 1394 1823 3441 3498 4314 1394
Size: 429 1618 57 816 3507
Fragments arranged by size:
3507 1618 816 429 57
BsrDI GCAATG_nn'
Cuts at: 65 1248 2274 2343 3483 4922 5096 65
Size: 1183 1026 69 1140 1439 174 1396
Fragments arranged by size:
1439 1396 1183 1140 1026 174 69
BsrFI r'CCGG_y
Cuts at: 1700 1810 2448 2638 3306 5071 5258 1700
Size: 110 638 190 668 1765 187 2869
Fragments arranged by size:
2869 1765 668 638 190 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 2375 4487 5499 2375
Size: 2112 1012 3303
Fragments arranged by size:
3303 2112 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2185 3485 794
Size: 1391 1300 3736
Fragments arranged by size:
3736 1391 1300
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 2713 3364 1184
Size: 1529 651 4247
Fragments arranged by size:
4247 1529 651
BstXI CCAn_nnnn'nTGG
Cuts at: 2467 2884 2467
Size: 417 6010
BstYI r'GATC_y
Cuts at: 1972 2487 2499 3033 3757 4124 4509 4526 5978
Size: 515 12 534 724 367 385 17 1452
Cuts at: 5978 6064 6076 6422 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 724 534 515 385 367 346
86 17 12 12
BtgI C'CryG_G
Cuts at: 513 1065 2250 2304 2365 2454 3631 513
Size: 552 1185 54 61 89 1177 3309
Fragments arranged by size:
3309 1185 1177 552 89 61 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2380 2446 3186 487
Size: 683 56 1154 66 740 3728
Fragments arranged by size:
3728 1154 740 683 66 56
BtsI GCAGTG_nn'
Cuts at: 2160 3303 3943 4737 4757 2160
Size: 1143 640 794 20 3830
Fragments arranged by size:
3830 1143 794 640 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6392
DraI TTT'AAA
Cuts at: 3731 3731
Size: 6427
DraIII CAC_nnn'GTG
Cuts at: 2604 2604
Size: 6427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5434 809
Size: 507 4118 1802
Fragments arranged by size:
4118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2457 3149 3436
Size: 54 1425 50 275 18 627 692 287
Cuts at: 3436 3874 4763 6392 8
Size: 438 889 1629 43
Fragments arranged by size:
1629 1425 889 692 627 438 287 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2960 4357 1353
Size: 1607 1397 3423
Fragments arranged by size:
3423 1607 1397
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5014 5388 5534 387
Size: 1064 138 3425 374 146 1280
Fragments arranged by size:
3425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2082 3005 3146 3549 4490 5087 5874
Size: 393 450 923 141 403 941 597 787
Cuts at: 5874 6142 1239
Size: 268 1524
Fragments arranged by size:
1524 941 923 787 597 450 403 393
268 141
EcoO109I rG'GnC_Cy
Cuts at: 2772 3019 2772
Size: 247 6180
EcoRI G'AATT_C
Cuts at: 3736 4114 3736
Size: 378 6049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3256 3256
Size: 6427
FspI TGC'GCA
Cuts at: 4933 4933
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 2314 3082 3341 5574 1678
Size: 636 768 259 2233 2531
Fragments arranged by size:
2531 2233 768 636 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2009 2041 2080 2112 2225
Size: 32 135 32 437 32 39 32 113
Cuts at: 2225 2257 2472 2504 2910 2942 5068 5100 5142
Size: 32 215 32 406 32 2126 32 42
Cuts at: 5142 5174 1373
Size: 32 2626
Fragments arranged by size:
2626 2126 437 406 215 135 113 42
39 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3771 4017 669
Size: 988 281 1833 246 3079
Fragments arranged by size:
3079 1833 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6427
HpaI GTT'AAC
Cuts at: 4017 4017
Size: 6427
KpnI G_GTAC'C
Cuts at: 3466 3752 3466
Size: 286 6141
MfeI C'AATT_G
Cuts at: 4026 4026
Size: 6427
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2502 3628 5541 5725 1305
Size: 1197 1126 1913 184 2007
Fragments arranged by size:
2007 1913 1197 1126 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2459 10
Size: 54 1425 343 627 3978
Fragments arranged by size:
3978 1425 627 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3005 3119 3419 3710 4385 4744 4903
Size: 648 1839 114 300 291 675 359 159
Cuts at: 4903 518
Size: 2042
Fragments arranged by size:
2042 1839 675 648 359 300 291 159
114
MspA1I CmG'CkG
Cuts at: 1606 2111 2185 2192 3313 3843 4520 5668 5913
Size: 505 74 7 1121 530 677 1148 245
Cuts at: 5913 1606
Size: 2120
Fragments arranged by size:
2120 1148 1121 677 530 505 245 74
7
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5875
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 3272 3785 5330 1132
Size: 97 2043 513 1545 2229
Fragments arranged by size:
2229 2043 1545 513 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6427
PciI A'CATG_T
Cuts at: 5326 5326
Size: 6427
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2155 2554 1108
Size: 1047 399 4981
Fragments arranged by size:
4981 1047 399
PfoI T'CCnGG_A
Cuts at: 2013 2098 2211 2406 2982 2013
Size: 85 113 195 576 5458
Fragments arranged by size:
5458 576 195 113 85
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3019 3019
Size: 6427
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6427
PsiI TTA'TAA
Cuts at: 3997 3997
Size: 6427
PspOMI G'GGCC_C
Cuts at: 2772 2772
Size: 6427
PstI C_TGCA'G
Cuts at: 830 2037 2508 3779 830
Size: 1207 471 1271 3478
Fragments arranged by size:
3478 1271 1207 471
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 2185 2192 3313 2185
Size: 7 1121 5299
Fragments arranged by size:
5299 1121 7
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SbfI CC_TGCA'GG
Cuts at: 2508 3779 2508
Size: 1271 5156
ScaI AGT'ACT
Cuts at: 4675 6387 4675
Size: 1712 4715
SexAI A'CCwGG_T
Cuts at: 3654 3654
Size: 6427
SfcI C'TryA_G
Cuts at: 826 1045 2033 2504 3775 4910 5591 5782 826
Size: 219 988 471 1271 1135 681 191 1471
Fragments arranged by size:
1471 1271 1135 988 681 471 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2135 2135
Size: 6427
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 2791 3281 3754 1872
Size: 919 490 473 4545
Fragments arranged by size:
4545 919 490 473
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2315 4531 5432 5694 820
Size: 197 926 372 2216 901 262 1553
Fragments arranged by size:
2216 1553 926 901 372 262 197
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3272 3785 1132
Size: 2140 513 3774
Fragments arranged by size:
3774 2140 513
SrfI GCCC'GGGC
Cuts at: 2791 2791
Size: 6427
SspI AAT'ATT
Cuts at: 5 52 2623 4351 5
Size: 47 2571 1728 2081
Fragments arranged by size:
2571 2081 1728 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6427
StyI C'CwwG_G
Cuts at: 513 1065 2475 3022 3231 3866 513
Size: 552 1410 547 209 635 3074
Fragments arranged by size:
3074 1410 635 552 547 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2031 2596 4454 4471 4624 4809 5254
Size: 1255 451 565 1858 17 153 185 445
Cuts at: 5254 325
Size: 1498
Fragments arranged by size:
1858 1498 1255 565 451 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4673 6385 96
Size: 274 80 33 51 1443 2696 1712 138
Fragments arranged by size:
2696 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3642 3674 6387 6419 3642
Size: 32 2713 32 3650
Fragments arranged by size:
3650 2713 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2849 1316
Size: 1533 4894
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3121 3187 3379 3573 3121
Size: 66 192 194 5975
Fragments arranged by size:
5975 194 192 66
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6427
XmaI C'CCGG_G
Cuts at: 1870 2789 3279 3752 1870
Size: 919 490 473 4545
Fragments arranged by size:
4545 919 490 473
XmnI GAAnn'nnTTC
Cuts at: 3050 4556 3050
Size: 1506 4921
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2809 4235 276
Size: 53 83 186 936 1275 1426 2468
Fragments arranged by size:
2468 1426 1275 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SfoI SwaI