Restriction map for
pFN21AE1324
(Circular) (Six-base) MAPSORT of: pFN21AE1324.seq Check: 3651 from: 1 to: 7147
pFN21AE1324 7147 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2417 2532 2715 4208 2417
Size: 115 183 1493 5356
Fragments arranged by size:
5356 1493 183 115
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4957 278
Size: 53 83 186 936 3421 2468
Fragments arranged by size:
3421 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4490 1937
Size: 2553 4594
Acc65I G'GTAC_C
Cuts at: 2301 4152 4396 4468 2301
Size: 1851 244 72 4980
Fragments arranged by size:
4980 1851 244 72
AclI AA'CG_TT
Cuts at: 1563 5274 5647 1563
Size: 3711 373 3063
Fragments arranged by size:
3711 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2135 2171 2468 3157 5210 6594 6862 1632
Size: 503 36 297 689 2053 1384 268 1917
Fragments arranged by size:
2053 1917 1384 689 503 297 268 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6950
AflIII A'CryG_T
Cuts at: 1203 6046 7023 1203
Size: 4843 977 1327
Fragments arranged by size:
4843 1327 977
AgeI A'CCGG_T
Cuts at: 5978 5978
Size: 7147
AhdI GACnn_n'nnGTC
Cuts at: 3998 5876 3998
Size: 1878 5269
AleI CACnn'nnGTG
Cuts at: 1166 2321 2538 1166
Size: 1155 217 5775
Fragments arranged by size:
5775 1155 217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2072 3863 1184
Size: 888 1791 4468
Fragments arranged by size:
4468 1791 888
AlwNI CAG_nnn'CTG
Cuts at: 2707 3180 3775 4020 4149 4376 4890 6462 2707
Size: 473 595 245 129 227 514 1572 3392
Fragments arranged by size:
3392 1572 595 514 473 245 227 129
ApaI G_GGCC'C
Cuts at: 2029 3196 4261 2029
Size: 1167 1065 4915
Fragments arranged by size:
4915 1167 1065
ApaLI G'TGCA_C
Cuts at: 3378 3848 5204 6360 3378
Size: 470 1356 1156 4165
Fragments arranged by size:
4165 1356 1156 470
ApoI r'AATT_y
Cuts at: 1493 3476 4456 4682 4834 6943 1493
Size: 1983 980 226 152 2109 1697
Fragments arranged by size:
2109 1983 1697 980 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5701 160
Size: 872 4669 1606
Fragments arranged by size:
4669 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7147
AvaI C'yCGr_G
Cuts at: 1870 1943 2122 2319 3660 4472 1870
Size: 73 179 197 1341 812 4545
Fragments arranged by size:
4545 1341 812 197 179 73
AvrII C'CTAG_G
Cuts at: 3520 3520
Size: 7147
BamHI G'GATC_C
Cuts at: 4477 4844 4477
Size: 367 6780
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2301 4042 4139 4152 4344
Size: 325 217 627 514 1741 97 13 192
Cuts at: 4344 4396 4468 5923 618
Size: 52 72 1455 1842
Fragments arranged by size:
1842 1741 1455 627 514 325 217 192
97 72 52 13
BanII G_rGCy'C
Cuts at: 1391 2029 2037 2133 2142 2208 2421 3196 3537
Size: 638 8 96 9 66 213 775 341
Cuts at: 3537 3622 3785 4261 4466 1391
Size: 85 163 476 205 4072
Fragments arranged by size:
4072 775 638 476 341 213 205 163
96 85 66 9 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6176
BbvCI CC'TCA_GC
Cuts at: 3368 4103 3368
Size: 735 6412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3966 4000 5338 5372 1903
Size: 34 50 34 1945 34 1338 34 3678
Fragments arranged by size:
3678 1945 1338 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5039 6249 5039
Size: 1210 5937
BclI T'GATC_A
Cuts at: 1548 2668 1548
Size: 1120 6027
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2065 3432 4265
Size: 107 122 71 631 605 393 1367 833
Cuts at: 4265 5758 136
Size: 1493 1525
Fragments arranged by size:
1525 1493 1367 833 631 605 393 122
107 71
BglII A'GATC_T
Cuts at: 2515 7142 2515
Size: 4627 2520
BlpI GC'TnA_GC
Cuts at: 4564 4564
Size: 7147
Bme1580I G_kGCm'C
Cuts at: 1790 2029 2545 2875 2908 3196 3382 3852 3967
Size: 239 516 330 33 288 186 470 115
Cuts at: 3967 4045 4162 4261 4349 5208 6364 1790
Size: 78 117 99 88 859 1156 2573
Fragments arranged by size:
2573 1156 859 516 470 330 288 239
186 117 115 99 88 78 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3493 3690 4878
Size: 416 53 83 399 24 2076 197 1188
Cuts at: 4878 5836 442
Size: 958 1753
Fragments arranged by size:
2076 1753 1188 958 416 399 197 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2270 2483 2810 3218 3629 3636 3731 4387
Size: 1031 213 327 408 411 7 95 656
Cuts at: 4387 5807 1239
Size: 1420 2579
Fragments arranged by size:
2579 1420 1031 656 411 408 327 213
95 7
Bpu10I CC'TnA_GC
Cuts at: 2202 2546 3368 4103 2202
Size: 344 822 735 5246
Fragments arranged by size:
5246 822 735 344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4172 5272 6137 6435 4172
Size: 1100 865 298 4884
Fragments arranged by size:
4884 1100 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2630 5810 882
Size: 617 1131 3180 2219
Fragments arranged by size:
3180 2219 1131 617
BsaAI yAC'GTr
Cuts at: 493 1204 4938 493
Size: 711 3734 2702
Fragments arranged by size:
3734 2702 711
BsaBI GATnn'nnATC
Cuts at: 7141 7141
Size: 7147
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2576 4954 5336 5971
Size: 53 83 186 936 1043 2378 382 635
Cuts at: 5971 275
Size: 1451
Fragments arranged by size:
2378 1451 1043 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5580 5978 6252 6399 6810 5580
Size: 398 274 147 411 5917
Fragments arranged by size:
5917 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3627 3657 1178
Size: 30 165 30 2224 30 4668
Fragments arranged by size:
4668 2224 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 3025 4046 4091 1186
Size: 922 917 1021 45 4242
Fragments arranged by size:
4242 1021 922 917 45
BseYI C'CCAG_C
Cuts at: 2802 2833 2907 3118 3588 3621 3768 3778 3843
Size: 31 74 211 470 33 147 10 65
Cuts at: 3843 3876 4038 6350 6846 2802
Size: 33 162 2312 496 3103
Fragments arranged by size:
3103 2312 496 470 211 162 147 74
65 33 33 31 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2389 3700 4039 4151 1163
Size: 573 653 1311 339 112 4159
Fragments arranged by size:
4159 1311 653 573 339 112
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4597 5358 6386 1540
Size: 275 186 2596 761 1028 2301
Fragments arranged by size:
2596 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2589 3382 3852 5208 5293 6364 2589
Size: 793 470 1356 85 1071 3372
Fragments arranged by size:
3372 1356 1071 793 470 85
BsmI GAATG_Cn'
Cuts at: 4658 4751 4658
Size: 93 7054
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2037 2133 2142 2208 2421 2545
Size: 399 239 8 96 9 66 213 124
Cuts at: 2545 2589 2875 2908 3196 3382 3537 3622 3785
Size: 44 286 33 288 186 155 85 163
Cuts at: 3785 3852 3967 4045 4162 4261 4349 4466 5208
Size: 67 115 78 117 99 88 117 742
Cuts at: 5208 5293 6364 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 288 286 239 213
186 163 155 124 117 117 115 99
96 88 85 85 78 67 66 44
33 9 8
BspEI T'CCGG_A
Cuts at: 6810 6810
Size: 7147
BspHI T'CATG_A
Cuts at: 5036 6766 5036
Size: 1730 5417
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2417 2532 2715 3137 3368 4208 4368 4502
Size: 1573 115 183 422 231 840 160 134
Cuts at: 4502 844
Size: 3489
Fragments arranged by size:
3489 1573 840 422 231 183 160 134
115
BsrBI CCG'CTC
Cuts at: 1394 1823 2071 5034 1394
Size: 429 248 2963 3507
Fragments arranged by size:
3507 2963 429 248
BsrDI GCAATG_nn'
Cuts at: 65 1248 5642 5816 65
Size: 1183 4394 174 1396
Fragments arranged by size:
4394 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4341 5791 5978 1700
Size: 110 2531 1450 187 2869
Fragments arranged by size:
2869 2531 1450 187 110
BsrGI T'GTAC_A
Cuts at: 96 2563 96
Size: 2467 4680
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7147
BssSI C'ACGA_G
Cuts at: 4269 5207 6219 4269
Size: 938 1012 5197
Fragments arranged by size:
5197 1012 938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3386 794
Size: 2592 4555
BstBI TT'CG_AA
Cuts at: 3237 5951 3237
Size: 2714 4433
BstEII G'GTnAC_C
Cuts at: 1184 4355 1184
Size: 3171 3976
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2312 2647 3022 3995 2011
Size: 301 335 375 973 5163
Fragments arranged by size:
5163 973 375 335 301
BstYI r'GATC_y
Cuts at: 1972 2515 3793 3952 4477 4844 5229 5246 6698
Size: 543 1278 159 525 367 385 17 1452
Cuts at: 6698 6784 6796 7142 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1278 543 525 385 367 346
159 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2210 2210
Size: 7147
BtgI C'CryG_G
Cuts at: 513 1065 3988 513
Size: 552 2923 3672
Fragments arranged by size:
3672 2923 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6408
Fragments arranged by size:
6408 683 56
BtsI GCAGTG_nn'
Cuts at: 3311 3446 3599 3871 4018 4319 4663 5457 5477
Size: 135 153 272 147 301 344 794 20
Cuts at: 5477 3311
Size: 4981
Fragments arranged by size:
4981 794 344 301 272 153 147 135
20
ClaI AT'CG_AT
Cuts at: 1554 3937 1554
Size: 2383 4764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3679 3714 529
Size: 35 3115 35 3962
Fragments arranged by size:
3962 3115 35 35
DraI TTT'AAA
Cuts at: 4451 4451
Size: 7147
DraIII CAC_nnn'GTG
Cuts at: 3386 3386
Size: 7147
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3405 6154 809
Size: 507 2089 2749 1802
Fragments arranged by size:
2749 2089 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2066 2952 4238
Size: 54 1425 50 275 18 236 886 1286
Cuts at: 4238 4594 5483 7112 8
Size: 356 889 1629 43
Fragments arranged by size:
1629 1425 1286 889 886 356 275 236
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4594 1537
Size: 275 2782 4090
Fragments arranged by size:
4090 2782 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2362 3239 5077 1353
Size: 1009 877 1838 3423
Fragments arranged by size:
3423 1838 1009 877
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5734 6108 6254 387
Size: 1064 138 4145 374 146 1280
Fragments arranged by size:
4145 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2171 2270 2468 2483 2810 3157
Size: 393 503 36 99 198 15 327 347
Cuts at: 3157 3218 3629 3636 3731 4387 5210 5807 6594
Size: 61 411 7 95 656 823 597 787
Cuts at: 6594 6862 1239
Size: 268 1524
Fragments arranged by size:
1524 823 787 656 597 503 411 393
347 327 268 198 99 95 61 36
15 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2177 2465 3453 2177
Size: 288 988 5871
Fragments arranged by size:
5871 988 288
EcoO109I rG'GnC_Cy
Cuts at: 2026 2119 2146 2471 2479 2489 3193 3611 3765
Size: 93 27 325 8 10 704 418 154
Cuts at: 3765 4193 4244 4257 4258 2026
Size: 428 51 13 1 4915
Fragments arranged by size:
4915 704 428 418 325 154 93 51
27 13 10 8 1
EcoRI G'AATT_C
Cuts at: 3476 4456 4834 3476
Size: 980 378 5789
Fragments arranged by size:
5789 980 378
FspI TGC'GCA
Cuts at: 5653 5653
Size: 7147
HaeII r_GCGC'y
Cuts at: 1678 2018 2605 4415 6294 1678
Size: 340 587 1810 1879 2531
Fragments arranged by size:
2531 1879 1810 587 340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3757 3789 3984 4016 5788
Size: 32 135 32 2185 32 195 32 1772
Cuts at: 5788 5820 5862 5894 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2185 1772 195 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4491 4737 669
Size: 988 281 2553 246 3079
Fragments arranged by size:
3079 2553 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7147
HpaI GTT'AAC
Cuts at: 4737 4737
Size: 7147
KpnI G_GTAC'C
Cuts at: 2305 4156 4400 4472 2305
Size: 1851 244 72 4980
Fragments arranged by size:
4980 1851 244 72
MfeI C'AATT_G
Cuts at: 4746 4746
Size: 7147
MluI A'CGCG_T
Cuts at: 7023 7023
Size: 7147
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2202 4448 6261 6445 1305
Size: 897 2246 1813 184 2007
Fragments arranged by size:
2246 2007 1813 897 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2954 10
Size: 54 1425 343 1122 4203
Fragments arranged by size:
4203 1425 1122 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 2538 2901 3970 3993 5105 5464
Size: 648 1155 217 363 1069 23 1112 359
Cuts at: 5464 5623 518
Size: 159 2042
Fragments arranged by size:
2042 1155 1112 1069 648 363 359 217
159 23
MspA1I CmG'CkG
Cuts at: 1606 2837 2911 3228 3367 3421 3629 3843 4563
Size: 1231 74 317 139 54 208 214 720
Cuts at: 4563 5240 6388 6633 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1231 1148 720 677 317 245 214
208 139 74 54
NcoI C'CATG_G
Cuts at: 513 1065 3988 513
Size: 552 2923 3672
Fragments arranged by size:
3672 2923 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7147
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7147
NotI GC'GGCC_GC
Cuts at: 4594 4594
Size: 7147
NruI TCG'CGA
Cuts at: 2506 2506
Size: 7147
NspI r_CATG'y
Cuts at: 1132 1229 3565 4505 6050 1132
Size: 97 2336 940 1545 2229
Fragments arranged by size:
2336 2229 1545 940 97
PasI CC'CwG_GG
Cuts at: 1348 2029 2593 4336 4370 1348
Size: 681 564 1743 34 4125
Fragments arranged by size:
4125 1743 681 564 34
PciI A'CATG_T
Cuts at: 6046 6046
Size: 7147
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3052 3064 3949 4149 4376 1108
Size: 1944 12 885 200 227 3879
Fragments arranged by size:
3879 1944 885 227 200 12
PfoI T'CCnGG_A
Cuts at: 2247 3210 3321 3789 2247
Size: 963 111 468 5605
Fragments arranged by size:
5605 963 468 111
PmeI GTTT'AAAC
Cuts at: 4451 4451
Size: 7147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5216 5248 6729 6761 5216
Size: 32 1481 32 5602
Fragments arranged by size:
5602 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2146 2479 2489 3611 3765 4193 4244 2146
Size: 333 10 1122 154 428 51 5049
Fragments arranged by size:
5049 1122 428 333 154 51 10
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7147
PsiI TTA'TAA
Cuts at: 4717 4717
Size: 7147
PspOMI G'GGCC_C
Cuts at: 2025 3192 4257 2025
Size: 1167 1065 4915
Fragments arranged by size:
4915 1167 1065
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2342 2374 2342
Size: 32 7115
PstI C_TGCA'G
Cuts at: 830 3185 3842 4499 830
Size: 2355 657 657 3478
Fragments arranged by size:
3478 2355 657 657
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7147
PvuII CAG'CTG
Cuts at: 2837 2911 3228 3367 3421 3843 2837
Size: 74 317 139 54 422 6141
Fragments arranged by size:
6141 422 317 139 74 54
SalI G'TCGA_C
Cuts at: 1936 4489 1936
Size: 2553 4594
SanDI GG'GwC_CC
Cuts at: 4193 4193
Size: 7147
SbfI CC_TGCA'GG
Cuts at: 4499 4499
Size: 7147
ScaI AGT'ACT
Cuts at: 5395 7107 5395
Size: 1712 5435
SexAI A'CCwGG_T
Cuts at: 2811 2811
Size: 7147
SfcI C'TryA_G
Cuts at: 826 1045 2258 3181 3838 4495 5630 6311 6502
Size: 219 1213 923 657 657 1135 681 191
Cuts at: 6502 826
Size: 1471
Fragments arranged by size:
1471 1213 1135 923 681 657 657 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7147
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7147
SmaI CCC'GGG
Cuts at: 1872 4474 1872
Size: 2602 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4187 5251 6152 6414 820
Size: 197 926 2244 1064 901 262 1553
Fragments arranged by size:
2244 1553 1064 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4938 493
Size: 4445 2702
SpeI A'CTAG_T
Cuts at: 152 2779 152
Size: 2627 4520
SphI G_CATG'C
Cuts at: 1132 3565 4505 1132
Size: 2433 940 3774
Fragments arranged by size:
3774 2433 940
SspI AAT'ATT
Cuts at: 5 52 5071 5
Size: 47 5019 2081
Fragments arranged by size:
5019 2081 47
StuI AGG'CCT
Cuts at: 1517 2795 3143 3165 1517
Size: 1278 348 22 5499
Fragments arranged by size:
5499 1278 348 22
StyI C'CwwG_G
Cuts at: 513 1065 2133 3520 3988 4310 4586 513
Size: 552 1068 1387 468 322 276 3074
Fragments arranged by size:
3074 1387 1068 552 468 322 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2383 3835 5174 5191 5344 5529 5974
Size: 1255 803 1452 1339 17 153 185 445
Cuts at: 5974 325
Size: 1498
Fragments arranged by size:
1498 1452 1339 1255 803 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2563 4206 5393
Size: 274 80 33 51 1443 586 1643 1187
Cuts at: 5393 7105 96
Size: 1712 138
Fragments arranged by size:
1712 1643 1443 1187 586 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2791 2823 3627 3659 7107 7139 2791
Size: 32 804 32 3448 32 2799
Fragments arranged by size:
3448 2799 804 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3332 4429 1316
Size: 2016 1097 4034
Fragments arranged by size:
4034 2016 1097
XbaI T'CTAG_A
Cuts at: 4483 4483
Size: 7147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2295 2915 3019 3083 2295
Size: 620 104 64 6359
Fragments arranged by size:
6359 620 104 64
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7147
XmaI C'CCGG_G
Cuts at: 1870 4472 1870
Size: 2602 4545
XmnI GAAnn'nnTTC
Cuts at: 5276 5276
Size: 7147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4955 276
Size: 53 83 186 936 3421 2468
Fragments arranged by size:
3421 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NsiI PacI PmlI PspXI RsrII
SacI SacII SapI SfiI SfoI SrfI SwaI