Restriction map for
pFN21AE1322
(Circular) (Six-base) MAPSORT of: pFN21AE1322.seq Check: 3971 from: 1 to: 5359
pFN21AE1322 5359 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2325 3169 278
Size: 53 83 186 936 789 844 2468
Fragments arranged by size:
2468 936 844 789 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2102 2702 1937
Size: 165 600 4594
Fragments arranged by size:
4594 600 165
Acc65I G'GTAC_C
Cuts at: 2680 2680
Size: 5359
AclI AA'CG_TT
Cuts at: 1563 3486 3859 1563
Size: 1923 373 3063
Fragments arranged by size:
3063 1923 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2393 2567 3422 4806 5074 1632
Size: 761 174 855 1384 268 1917
Fragments arranged by size:
1917 1384 855 761 268 174
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5162
AflIII A'CryG_T
Cuts at: 1203 4258 5235 1203
Size: 3055 977 1327
Fragments arranged by size:
3055 1327 977
AgeI A'CCGG_T
Cuts at: 4190 4190
Size: 5359
AhdI GACnn_n'nnGTC
Cuts at: 4088 4088
Size: 5359
AleI CACnn'nnGTG
Cuts at: 1166 2636 1166
Size: 1470 3889
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5359
AlwNI CAG_nnn'CTG
Cuts at: 2143 2346 2428 3102 4674 2143
Size: 203 82 674 1572 2828
Fragments arranged by size:
2828 1572 674 203 82
ApaLI G'TGCA_C
Cuts at: 3416 4572 3416
Size: 1156 4203
ApoI r'AATT_y
Cuts at: 1493 2153 2668 2894 3046 5155 1493
Size: 660 515 226 152 2109 1697
Fragments arranged by size:
2109 1697 660 515 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3913 160
Size: 872 2881 1606
Fragments arranged by size:
2881 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
AvaI C'yCGr_G
Cuts at: 1870 1943 2684 1870
Size: 73 741 4545
Fragments arranged by size:
4545 741 73
BamHI G'GATC_C
Cuts at: 2689 3056 2689
Size: 367 4992
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2680 4135 618
Size: 325 217 627 893 1455 1842
Fragments arranged by size:
1842 1455 893 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2206 2520 2678 1391
Size: 815 314 158 4072
Fragments arranged by size:
4072 815 314 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3550 3584 1903
Size: 34 1613 34 3678
Fragments arranged by size:
3678 1613 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3251 4461 3251
Size: 1210 4149
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5359
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3970 136
Size: 107 122 71 631 605 2298 1525
Fragments arranged by size:
2298 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2583 5354 2583
Size: 2771 2588
BlpI GC'TnA_GC
Cuts at: 2776 2776
Size: 5359
Bme1580I G_kGCm'C
Cuts at: 1790 3420 4576 1790
Size: 1630 1156 2573
Fragments arranged by size:
2573 1630 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5359
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2027 2035 3090
Size: 416 53 83 399 24 610 8 1055
Cuts at: 3090 4048 442
Size: 958 1753
Fragments arranged by size:
1753 1055 958 610 416 399 83 53
24 8
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5359
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 5359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4019 1239
Size: 2780 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2065 3484 4349 4647 2065
Size: 1419 865 298 2777
Fragments arranged by size:
2777 1419 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4022 882
Size: 617 2523 2219
Fragments arranged by size:
2523 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3150 493
Size: 711 1946 2702
Fragments arranged by size:
2702 1946 711
BsaBI GATnn'nnATC
Cuts at: 5353 5353
Size: 5359
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2322 2532 3166 3548
Size: 53 83 186 936 789 210 634 382
Cuts at: 3548 4183 275
Size: 635 1451
Fragments arranged by size:
1451 936 789 635 634 382 210 186
83 53
BsaWI w'CCGG_w
Cuts at: 3792 4190 4464 4611 5022 3792
Size: 398 274 147 411 4129
Fragments arranged by size:
4129 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 1178
Size: 30 165 30 1140 30 3964
Fragments arranged by size:
3964 1140 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2294 1186
Size: 1108 4251
BseYI C'CCAG_C
Cuts at: 2161 4562 5058 2161
Size: 2401 496 2462
Fragments arranged by size:
2462 2401 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4786
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2809 3570 4598 1540
Size: 275 186 808 761 1028 2301
Fragments arranged by size:
2301 1028 808 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3420 3505 4576 3420
Size: 85 1071 4203
Fragments arranged by size:
4203 1071 85
BsmI GAATG_Cn'
Cuts at: 2870 2963 2870
Size: 93 5266
BsmBI CGTCTCn'nnnn_
Cuts at: 2329 2329
Size: 5359
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2206 2520 2678 3420 3505 4576 1391
Size: 399 416 314 158 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 416 399 314 158 85
BspEI T'CCGG_A
Cuts at: 5022 5022
Size: 5359
BspHI T'CATG_A
Cuts at: 3248 4978 3248
Size: 1730 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2714 844
Size: 1870 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3246 1394
Size: 429 1423 3507
Fragments arranged by size:
3507 1423 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2241 3854 4028 65
Size: 1183 993 1613 174 1396
Fragments arranged by size:
1613 1396 1183 993 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4003 4190 1700
Size: 110 2193 187 2869
Fragments arranged by size:
2869 2193 187 110
BsrGI T'GTAC_A
Cuts at: 96 2628 96
Size: 2532 2827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5359
BssSI C'ACGA_G
Cuts at: 3419 4431 3419
Size: 1012 4347
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5359
BstBI TT'CG_AA
Cuts at: 4163 4163
Size: 5359
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5359
BstXI CCAn_nnnn'nTGG
Cuts at: 2017 2335 2527 2017
Size: 318 192 4849
Fragments arranged by size:
4849 318 192
BstYI r'GATC_y
Cuts at: 1972 2583 2689 3056 3441 3458 4910 4996 5008
Size: 611 106 367 385 17 1452 86 12
Cuts at: 5008 5354 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 611 385 367 346 106 86
17 12
BstZ17I GTA'TAC
Cuts at: 2103 2103
Size: 5359
BtgI C'CryG_G
Cuts at: 513 1065 2035 2441 513
Size: 552 970 406 3431
Fragments arranged by size:
3431 970 552 406
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4620
Fragments arranged by size:
4620 683 56
BtsI GCAGTG_nn'
Cuts at: 2875 3669 3689 2875
Size: 794 20 4545
Fragments arranged by size:
4545 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2245 2280 529
Size: 35 1681 35 3608
Fragments arranged by size:
3608 1681 35 35
DraI TTT'AAA
Cuts at: 2663 2663
Size: 5359
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4366 809
Size: 507 3050 1802
Fragments arranged by size:
3050 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2337 2806 3695
Size: 54 1425 50 275 18 507 469 889
Cuts at: 3695 5324 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 507 469 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2806 1537
Size: 275 994 4090
Fragments arranged by size:
4090 994 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2271 2429 3289 1353
Size: 918 158 860 3423
Fragments arranged by size:
3423 918 860 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2045 3946 4320 4466 387
Size: 1064 138 456 1901 374 146 1280
Fragments arranged by size:
1901 1280 1064 456 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2393 2567 3422 4019 4806 5074 1239
Size: 393 761 174 855 597 787 268 1524
Fragments arranged by size:
1524 855 787 761 597 393 268 174
EcoRI G'AATT_C
Cuts at: 2668 3046 2668
Size: 378 4981
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 5359
FspI TGC'GCA
Cuts at: 3865 3865
Size: 5359
HaeII r_GCGC'y
Cuts at: 1678 4506 1678
Size: 2828 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2445 2477 2543 2575 4000
Size: 32 135 32 873 32 66 32 1425
Cuts at: 4000 4032 4074 4106 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1425 873 135 66 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 2949 669
Size: 988 281 765 246 3079
Fragments arranged by size:
3079 988 765 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5359
HpaI GTT'AAC
Cuts at: 2949 2949
Size: 5359
KpnI G_GTAC'C
Cuts at: 2684 2684
Size: 5359
MfeI C'AATT_G
Cuts at: 2958 2958
Size: 5359
MluI A'CGCG_T
Cuts at: 5235 5235
Size: 5359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2431 4473 4657 1305
Size: 1126 2042 184 2007
Fragments arranged by size:
2042 2007 1126 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2339 10
Size: 54 1425 343 507 3030
Fragments arranged by size:
3030 1425 507 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2636 3317 3676 3835 518
Size: 648 843 627 681 359 159 2042
Fragments arranged by size:
2042 843 681 648 627 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2335 2775 3452 4600 4845 1606
Size: 431 298 440 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 440 431 298 245
NcoI C'CATG_G
Cuts at: 513 1065 2441 513
Size: 552 1376 3431
Fragments arranged by size:
3431 1376 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5359
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5359
NotI GC'GGCC_GC
Cuts at: 2806 2806
Size: 5359
NspI r_CATG'y
Cuts at: 1132 1229 2717 4262 1132
Size: 97 1488 1545 2229
Fragments arranged by size:
2229 1545 1488 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5359
PciI A'CATG_T
Cuts at: 4258 4258
Size: 5359
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2317 1108
Size: 1209 4150
PfoI T'CCnGG_A
Cuts at: 2064 2064
Size: 5359
PmeI GTTT'AAAC
Cuts at: 2663 2663
Size: 5359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 4941 4973 3428
Size: 32 1481 32 3814
Fragments arranged by size:
3814 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5359
PsiI TTA'TAA
Cuts at: 2929 2929
Size: 5359
PstI C_TGCA'G
Cuts at: 830 2711 830
Size: 1881 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5359
PvuII CAG'CTG
Cuts at: 2335 2335
Size: 5359
SacII CC_GC'GG
Cuts at: 2038 2038
Size: 5359
SalI G'TCGA_C
Cuts at: 1936 2701 1936
Size: 765 4594
SbfI CC_TGCA'GG
Cuts at: 2711 2711
Size: 5359
ScaI AGT'ACT
Cuts at: 3607 5319 3607
Size: 1712 3647
SfcI C'TryA_G
Cuts at: 826 1045 2350 2707 3842 4523 4714 826
Size: 219 1305 357 1135 681 191 1471
Fragments arranged by size:
1471 1305 1135 681 357 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5359
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5359
SmaI CCC'GGG
Cuts at: 1872 2686 1872
Size: 814 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 3463 4364 4626 820
Size: 197 926 137 1383 901 262 1553
Fragments arranged by size:
1553 1383 926 901 262 197 137
SnaBI TAC'GTA
Cuts at: 493 3150 493
Size: 2657 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5359
SphI G_CATG'C
Cuts at: 1132 2717 1132
Size: 1585 3774
SspI AAT'ATT
Cuts at: 5 52 3283 5
Size: 47 3231 2081
Fragments arranged by size:
3231 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5359
StyI C'CwwG_G
Cuts at: 513 1065 2441 2798 513
Size: 552 1376 357 3074
Fragments arranged by size:
3074 1376 552 357
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 3386 3403 3556 3741 4186 325
Size: 1255 651 1155 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1155 651 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2628 3605 5317
Size: 274 80 33 51 1443 651 977 1712
Cuts at: 5317 96
Size: 138
Fragments arranged by size:
1712 1443 977 651 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5319 5351 5319
Size: 32 5327
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5359
XbaI T'CTAG_A
Cuts at: 2695 2695
Size: 5359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5359
XmaI C'CCGG_G
Cuts at: 1870 2684 1870
Size: 814 4545
XmnI GAAnn'nnTTC
Cuts at: 2552 3488 2552
Size: 936 4423
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2323 3167 276
Size: 53 83 186 936 789 844 2468
Fragments arranged by size:
2468 936 844 789 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI