Restriction map for
pFN21AE1320
(Circular) (Six-base) MAPSORT of: pFN21AE1320.seq Check: 7484 from: 1 to: 5998
pFN21AE1320 5998 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 4125 4498 1563
Size: 2562 373 3063
Fragments arranged by size:
3063 2562 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2205 2294 3014 4061 5445 5713 1632
Size: 573 89 720 1047 1384 268 1917
Fragments arranged by size:
1917 1384 1047 720 573 268 89
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 2611 4727 2611
Size: 2116 3882
AleI CACnn'nnGTG
Cuts at: 1166 2540 3233 1166
Size: 1374 693 3931
Fragments arranged by size:
3931 1374 693
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5998
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 5966
AlwNI CAG_nnn'CTG
Cuts at: 3741 5313 3741
Size: 1572 4426
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 3307 3533 3685 5794 1493
Size: 1814 226 152 2109 1697
Fragments arranged by size:
2109 1814 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 2911 2953 3014 3323 1870
Size: 73 624 344 42 61 309 4545
Fragments arranged by size:
4545 624 344 309 73 61 42
BamHI G'GATC_C
Cuts at: 2264 3328 3695 2264
Size: 1064 367 4567
Fragments arranged by size:
4567 1064 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2546 2563 3319 4774 618
Size: 325 217 627 759 17 756 1455 1842
Fragments arranged by size:
1842 1455 759 756 627 325 217 17
BanII G_rGCy'C
Cuts at: 1391 2105 2865 2918 3028 3317 1391
Size: 714 760 53 110 289 4072
Fragments arranged by size:
4072 760 714 289 110 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5027
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4189 4223 1903
Size: 34 2252 34 3678
Fragments arranged by size:
3678 2252 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2272 3890 5100 2272
Size: 1618 1210 3170
Fragments arranged by size:
3170 1618 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 4609 136
Size: 107 122 71 631 605 365 2572 1525
Fragments arranged by size:
2572 1525 631 605 365 122 107 71
BglII A'GATC_T
Cuts at: 2329 5993 2329
Size: 3664 2334
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 2549 3215 4059 5215 1790
Size: 759 666 844 1156 2573
Fragments arranged by size:
2573 1156 844 759 666
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3729 4687 442
Size: 416 53 83 399 24 2312 958 1753
Fragments arranged by size:
2312 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3285 3285
Size: 5998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3242 4658 1239
Size: 2003 1416 2579
Fragments arranged by size:
2579 2003 1416
Bpu10I CC'TnA_GC
Cuts at: 2799 2799
Size: 5998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2729 4123 4988 5286 2729
Size: 1394 865 298 3441
Fragments arranged by size:
3441 1394 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3789 493
Size: 711 2585 2702
Fragments arranged by size:
2702 2585 711
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3805 4187 4822 275
Size: 53 83 186 936 2272 382 635 1451
Fragments arranged by size:
2272 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4431 4829 5103 5250 5661 4431
Size: 398 274 147 411 4768
Fragments arranged by size:
4768 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2900 2930 3194 3224 1178
Size: 30 165 30 1497 30 264 30 3952
Fragments arranged by size:
3952 1497 264 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3102 3218 1186
Size: 1916 116 3966
Fragments arranged by size:
3966 1916 116
BseYI C'CCAG_C
Cuts at: 3240 5201 5697 3240
Size: 1961 496 3541
Fragments arranged by size:
3541 1961 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2042 2654 3269 1163
Size: 573 306 612 615 3892
Fragments arranged by size:
3892 615 612 573 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2865 2918 3028 4059 4144 5215 2865
Size: 53 110 1031 85 1071 3648
Fragments arranged by size:
3648 1071 1031 110 85 53
BsmI GAATG_Cn'
Cuts at: 3509 3602 3509
Size: 93 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2105 2549 2865 2918 3028 3215 3317
Size: 399 315 444 316 53 110 187 102
Cuts at: 3317 4059 4144 5215 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 444 399 316 315 187
110 102 85 53
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 3887 5617 3887
Size: 1730 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3353 844
Size: 2509 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3885 1394
Size: 429 2062 3507
Fragments arranged by size:
3507 2062 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2839 4493 4667 65
Size: 1183 1591 1654 174 1396
Fragments arranged by size:
1654 1591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4642 4829 1700
Size: 110 2832 187 2869
Fragments arranged by size:
2869 2832 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 3245 4058 5070 3245
Size: 813 1012 4173
Fragments arranged by size:
4173 1012 813
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5998
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstYI r'GATC_y
Cuts at: 1972 2264 2329 2979 3001 3328 3695 4080 4097
Size: 292 65 650 22 327 367 385 17
Cuts at: 4097 5549 5635 5647 5993 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 650 385 367 346 327 292
86 65 22 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3231 513
Size: 552 939 1227 3280
Fragments arranged by size:
3280 1227 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5259
Fragments arranged by size:
5259 683 56
BtsI GCAGTG_nn'
Cuts at: 2793 2968 3514 4308 4328 2793
Size: 175 546 794 20 4463
Fragments arranged by size:
4463 794 546 175 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2700 2735 529
Size: 35 2136 35 3792
Fragments arranged by size:
3792 2136 35 35
DraI TTT'AAA
Cuts at: 3302 3302
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2445 2503 2944
Size: 54 1425 50 275 18 615 58 441
Cuts at: 2944 3445 4334 5963 8
Size: 501 889 1629 43
Fragments arranged by size:
1629 1425 889 615 501 441 275 58
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2061 3033 3131 3168 3928 1353
Size: 708 972 98 37 760 3423
Fragments arranged by size:
3423 972 760 708 98 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2501 4585 4959 5105 387
Size: 1064 138 912 2084 374 146 1280
Fragments arranged by size:
2084 1280 1064 912 374 146 138
EcoICRI GAG'CTC
Cuts at: 2863 2916 3026 2863
Size: 53 110 5835
Fragments arranged by size:
5835 110 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2205 2294 3014 3242 4061 4658 5445
Size: 393 573 89 720 228 819 597 787
Cuts at: 5445 5713 1239
Size: 268 1524
Fragments arranged by size:
1524 819 787 720 597 573 393 268
228 89
EcoNI CCTnn'n_nnAGG
Cuts at: 3200 3200
Size: 5998
EcoRI G'AATT_C
Cuts at: 3307 3685 3307
Size: 378 5620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 5998
FspI TGC'GCA
Cuts at: 4504 4504
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 5145 1678
Size: 3467 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2155 2187 2597 2629 2900
Size: 32 135 32 583 32 410 32 271
Cuts at: 2900 2932 3174 3206 4639 4671 4713 4745 1373
Size: 32 242 32 1433 32 42 32 2626
Fragments arranged by size:
2626 1433 583 410 271 242 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5998
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2700 2952 3249 5112 5296 1305
Size: 1395 252 297 1863 184 2007
Fragments arranged by size:
2007 1863 1395 297 252 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2505 2946 10
Size: 54 1425 343 673 441 3062
Fragments arranged by size:
3062 1425 673 441 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 3233 3956 4315 4474 518
Size: 648 1374 693 723 359 159 2042
Fragments arranged by size:
2042 1374 723 693 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2509 3253 3414 4091 5239 5484 1606
Size: 903 744 161 677 1148 245 2120
Fragments arranged by size:
2120 1148 903 744 677 245 161
NcoI C'CATG_G
Cuts at: 513 1065 2004 3231 513
Size: 552 939 1227 3280
Fragments arranged by size:
3280 1227 939 552
NdeI CA'TA_TG
Cuts at: 387 2245 387
Size: 1858 4140
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 2044 3356 4901 1132
Size: 97 815 1312 1545 2229
Fragments arranged by size:
2229 1545 1312 815 97
PasI CC'CwG_GG
Cuts at: 1348 2617 1348
Size: 1269 4729
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5998
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 3568 3568
Size: 5998
PspXI vC'TCGA_Gb
Cuts at: 2911 2911
Size: 5998
PstI C_TGCA'G
Cuts at: 830 3350 830
Size: 2520 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
PvuII CAG'CTG
Cuts at: 2509 2509
Size: 5998
SacI G_AGCT'C
Cuts at: 2865 2918 3028 2865
Size: 53 110 5835
Fragments arranged by size:
5835 110 53
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SapI GCTCTTCn'nnn_
Cuts at: 2061 3033 3131 3168 2061
Size: 972 98 37 4891
Fragments arranged by size:
4891 972 98 37
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SfcI C'TryA_G
Cuts at: 826 1045 2731 3346 4481 5162 5353 826
Size: 219 1686 615 1135 681 191 1471
Fragments arranged by size:
1686 1471 1135 681 615 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 3325 1872
Size: 1453 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2744 2911 3014 4102 5003 5265
Size: 197 926 801 167 103 1088 901 262
Cuts at: 5265 820
Size: 1553
Fragments arranged by size:
1553 1088 926 901 801 262 197 167
103
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 2044 3356 1132
Size: 912 1312 3774
Fragments arranged by size:
3774 1312 912
SspI AAT'ATT
Cuts at: 5 52 2258 3922 5
Size: 47 2206 1664 2081
Fragments arranged by size:
2206 2081 1664 47
StuI AGG'CCT
Cuts at: 1517 2762 3079 1517
Size: 1245 317 4436
Fragments arranged by size:
4436 1245 317
StyI C'CwwG_G
Cuts at: 513 1065 2004 3231 3437 513
Size: 552 939 1227 206 3074
Fragments arranged by size:
3074 1227 939 552 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2534 2873 4025 4042 4195 4380 4825
Size: 1255 954 339 1152 17 153 185 445
Cuts at: 4825 325
Size: 1498
Fragments arranged by size:
1498 1255 1152 954 445 339 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4244 5956 96
Size: 274 80 33 51 1443 2267 1712 138
Fragments arranged by size:
2267 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3194 3226 5958 5990 3194
Size: 32 2732 32 3202
Fragments arranged by size:
3202 2732 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 2911 3014 1943
Size: 968 103 4927
Fragments arranged by size:
4927 968 103
XmaI C'CCGG_G
Cuts at: 1870 3323 1870
Size: 1453 4545
XmnI GAAnn'nnTTC
Cuts at: 2537 4127 2537
Size: 1590 4408
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI