Restriction map for
pFN21AE1317
(Circular) (Six-base) MAPSORT of: pFN21AE1317.seq Check: 9072 from: 1 to: 6919
pFN21AE1317 6919 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3420 4729 278
Size: 53 83 186 936 1884 1309 2468
Fragments arranged by size:
2468 1884 1309 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4262 1937
Size: 2325 4594
Acc65I G'GTAC_C
Cuts at: 4240 4240
Size: 6919
AclI AA'CG_TT
Cuts at: 1563 5046 5419 1563
Size: 3483 373 3063
Fragments arranged by size:
3483 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2316 3432 4982 6366 6634 1632
Size: 684 1116 1550 1384 268 1917
Fragments arranged by size:
1917 1550 1384 1116 684 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6722
AflIII A'CryG_T
Cuts at: 1203 2910 5818 6795 1203
Size: 1707 2908 977 1327
Fragments arranged by size:
2908 1707 1327 977
AgeI A'CCGG_T
Cuts at: 5750 5750
Size: 6919
AhdI GACnn_n'nnGTC
Cuts at: 5648 5648
Size: 6919
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3460 1184
Size: 2276 4643
AlwNI CAG_nnn'CTG
Cuts at: 2224 4662 6234 2224
Size: 2438 1572 2909
Fragments arranged by size:
2909 2438 1572
ApaI G_GGCC'C
Cuts at: 2023 2023
Size: 6919
ApaLI G'TGCA_C
Cuts at: 2770 4976 6132 2770
Size: 2206 1156 3557
Fragments arranged by size:
3557 2206 1156
ApoI r'AATT_y
Cuts at: 1493 2548 2563 2579 4228 4454 4606 6715 1493
Size: 1055 15 16 1649 226 152 2109 1697
Fragments arranged by size:
2109 1697 1649 1055 226 152 16 15
AseI AT'TA_AT
Cuts at: 160 1032 5473 160
Size: 872 4441 1606
Fragments arranged by size:
4441 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6919
AvaI C'yCGr_G
Cuts at: 1870 1943 2553 4244 1870
Size: 73 610 1691 4545
Fragments arranged by size:
4545 1691 610 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3084 3117 3084
Size: 33 6886
BamHI G'GATC_C
Cuts at: 2643 4249 4616 2643
Size: 1606 367 4946
Fragments arranged by size:
4946 1606 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4240 5695 618
Size: 325 217 627 2453 1455 1842
Fragments arranged by size:
2453 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2023 2755 4238 1391
Size: 632 732 1483 4072
Fragments arranged by size:
4072 1483 732 632
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3420 928
Size: 971 1521 4427
Fragments arranged by size:
4427 1521 971
BbvCI CC'TCA_GC
Cuts at: 2211 4093 2211
Size: 1882 5037
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5110 5144 1903
Size: 34 50 34 3089 34 3678
Fragments arranged by size:
3678 3089 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4811 6021 4811
Size: 1210 5709
BclI T'GATC_A
Cuts at: 1548 2739 3837 3852 1548
Size: 1191 1098 15 4615
Fragments arranged by size:
4615 1191 1098 15
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 3665 5530
Size: 107 122 71 631 605 355 1638 1865
Cuts at: 5530 136
Size: 1525
Fragments arranged by size:
1865 1638 1525 631 605 355 122 107
71
BglII A'GATC_T
Cuts at: 4111 6914 4111
Size: 2803 4116
BlpI GC'TnA_GC
Cuts at: 2754 4336 2754
Size: 1582 5337
Bme1580I G_kGCm'C
Cuts at: 1790 2023 2397 2774 4980 6136 1790
Size: 233 374 377 2206 1156 2573
Fragments arranged by size:
2573 2206 1156 377 374 233
BmgBI CAC'GTC
Cuts at: 1318 2203 2242 4066 1318
Size: 885 39 1824 4171
Fragments arranged by size:
4171 1824 885 39
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2391 3250 4650
Size: 416 53 83 399 24 974 859 1400
Cuts at: 4650 5608 442
Size: 958 1753
Fragments arranged by size:
1753 1400 974 958 859 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6919
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2774 2774
Size: 6919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3048 3570 3733 5579 1239
Size: 1809 522 163 1846 2579
Fragments arranged by size:
2579 1846 1809 522 163
Bpu10I CC'TnA_GC
Cuts at: 2211 4093 2211
Size: 1882 5037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2099 3096 5044 5909 6207 2099
Size: 997 1948 865 298 2811
Fragments arranged by size:
2811 1948 997 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2550 5582 882
Size: 617 1051 3032 2219
Fragments arranged by size:
3032 2219 1051 617
BsaAI yAC'GTr
Cuts at: 493 1204 2913 4710 493
Size: 711 1709 1797 2702
Fragments arranged by size:
2702 1797 1709 711
BsaBI GATnn'nnATC
Cuts at: 3521 6913 3521
Size: 3392 3527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3417 4726 5108 5743
Size: 53 83 186 936 1884 1309 382 635
Cuts at: 5743 275
Size: 1451
Fragments arranged by size:
1884 1451 1309 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2301 2732 4152 5352 5750 6024 6171 6582 2301
Size: 431 1420 1200 398 274 147 411 2638
Fragments arranged by size:
2638 1420 1200 431 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2243 2246 2273 2276 3695
Size: 30 165 30 840 3 27 3 1419
Cuts at: 3695 3725 3731 3761 1178
Size: 30 6 30 4336
Fragments arranged by size:
4336 1419 840 165 30 30 30 30
27 6 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2251 1186
Size: 1065 5854
BseYI C'CCAG_C
Cuts at: 3231 6122 6618 3231
Size: 2891 496 3532
Fragments arranged by size:
3532 2891 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3145 3703 4088 1163
Size: 573 1409 558 385 3994
Fragments arranged by size:
3994 1409 573 558 385
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4369 5130 6158 1540
Size: 275 186 2368 761 1028 2301
Fragments arranged by size:
2368 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2276 2755 2774 2898 3061 4980 5065 6136 2276
Size: 479 19 124 163 1919 85 1071 3059
Fragments arranged by size:
3059 1919 1071 479 163 124 85 19
BsmI GAATG_Cn'
Cuts at: 4430 4523 4430
Size: 93 6826
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2276 2397 2755 2774 2898 3061
Size: 399 233 253 121 358 19 124 163
Cuts at: 3061 4238 4980 5065 6136 1391
Size: 1177 742 85 1071 2174
Fragments arranged by size:
2174 1177 1071 742 399 358 253 233
163 124 121 85 19
BspEI T'CCGG_A
Cuts at: 2301 4152 6582 2301
Size: 1851 2430 2638
Fragments arranged by size:
2638 2430 1851
BspHI T'CATG_A
Cuts at: 4808 6538 4808
Size: 1730 5189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3158 4274 844
Size: 2314 1116 3489
Fragments arranged by size:
3489 2314 1116
BsrBI CCG'CTC
Cuts at: 1394 1823 4806 1394
Size: 429 2983 3507
Fragments arranged by size:
3507 2983 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3575 3840 3855 4029 5414 5588 65
Size: 1183 2327 265 15 174 1385 174 1396
Fragments arranged by size:
2327 1396 1385 1183 265 174 174 15
BsrFI r'CCGG_y
Cuts at: 1700 1810 5563 5750 1700
Size: 110 3753 187 2869
Fragments arranged by size:
3753 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4140 96
Size: 4044 2875
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6919
BssSI C'ACGA_G
Cuts at: 4979 5991 4979
Size: 1012 5907
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2221 794
Size: 1427 5492
BstBI TT'CG_AA
Cuts at: 5723 5723
Size: 6919
BstEII G'GTnAC_C
Cuts at: 1184 2683 1184
Size: 1499 5420
BstYI r'GATC_y
Cuts at: 1972 2304 2643 3587 3964 4111 4249 4616 5001
Size: 332 339 944 377 147 138 367 385
Cuts at: 5001 5018 6470 6556 6568 6914 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 944 385 377 367 346 339
332 147 138 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2886 3797 4173 513
Size: 552 1821 911 376 3259
Fragments arranged by size:
3259 1821 911 552 376
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6180
Fragments arranged by size:
6180 683 56
BtsI GCAGTG_nn'
Cuts at: 2177 2361 3162 3825 4435 5229 5249 2177
Size: 184 801 663 610 794 20 3847
Fragments arranged by size:
3847 801 794 663 610 184 20
ClaI AT'CG_AT
Cuts at: 1554 3302 1554
Size: 1748 5171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3519 3554 529
Size: 35 2955 35 3894
Fragments arranged by size:
3894 2955 35 35
DraI TTT'AAA
Cuts at: 3339 4223 3339
Size: 884 6035
DraIII CAC_nnn'GTG
Cuts at: 2037 2037
Size: 6919
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3182 5926 809
Size: 507 1866 2744 1802
Fragments arranged by size:
2744 1866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2883 3861 4366
Size: 54 1425 50 275 18 1053 978 505
Cuts at: 4366 5255 6884 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1053 978 889 505 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4366 1537
Size: 275 2554 4090
Fragments arranged by size:
4090 2554 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 2264 4849 1353
Size: 764 147 2585 3423
Fragments arranged by size:
3423 2585 764 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5506 5880 6026 387
Size: 1064 138 3917 374 146 1280
Fragments arranged by size:
3917 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2753 2753
Size: 6919
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2316 3048 3432 3570 3733 4982 5579
Size: 393 684 732 384 138 163 1249 597
Cuts at: 5579 6366 6634 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1249 787 732 684 597 393 384
268 163 138
EcoNI CCTnn'n_nnAGG
Cuts at: 2795 2795
Size: 6919
EcoO109I rG'GnC_Cy
Cuts at: 2479 2479
Size: 6919
EcoRI G'AATT_C
Cuts at: 2548 2579 4228 4606 2548
Size: 31 1649 378 4861
Fragments arranged by size:
4861 1649 378 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4223 4223
Size: 6919
FspI TGC'GCA
Cuts at: 5425 5425
Size: 6919
HaeII r_GCGC'y
Cuts at: 1678 6066 1678
Size: 4388 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3267 3299 3829 3861 3944
Size: 32 135 32 1695 32 530 32 83
Cuts at: 3944 3976 5560 5592 5634 5666 1373
Size: 32 1584 32 42 32 2626
Fragments arranged by size:
2626 1695 1584 530 135 83 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2116 2455 2962 4263 4509 669
Size: 988 281 178 339 507 1301 246 3079
Fragments arranged by size:
3079 1301 988 507 339 281 246 178
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6919
HpaI GTT'AAC
Cuts at: 4509 4509
Size: 6919
KpnI G_GTAC'C
Cuts at: 4244 4244
Size: 6919
MfeI C'AATT_G
Cuts at: 2496 4518 2496
Size: 2022 4897
MluI A'CGCG_T
Cuts at: 6795 6795
Size: 6919
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3120 3969 3991 6033 6217 1305
Size: 1815 849 22 2042 184 2007
Fragments arranged by size:
2042 2007 1815 849 184 22
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2885 10
Size: 54 1425 343 1053 4044
Fragments arranged by size:
4044 1425 1053 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2326 2735 2780 2885 3833 4877 5236
Size: 648 1160 409 45 105 948 1044 359
Cuts at: 5236 5395 518
Size: 159 2042
Fragments arranged by size:
2042 1160 1044 948 648 409 359 159
105 45
MspA1I CmG'CkG
Cuts at: 1606 4097 4335 5012 6160 6405 1606
Size: 2491 238 677 1148 245 2120
Fragments arranged by size:
2491 2120 1148 677 245 238
NcoI C'CATG_G
Cuts at: 513 1065 2886 3797 513
Size: 552 1821 911 3635
Fragments arranged by size:
3635 1821 911 552
NdeI CA'TA_TG
Cuts at: 387 2649 387
Size: 2262 4657
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6919
NotI GC'GGCC_GC
Cuts at: 4366 4366
Size: 6919
NspI r_CATG'y
Cuts at: 1132 1229 4277 5822 1132
Size: 97 3048 1545 2229
Fragments arranged by size:
3048 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2403 1348
Size: 1055 5864
PciI A'CATG_T
Cuts at: 5818 5818
Size: 6919
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2037 2380 1108
Size: 929 343 5647
Fragments arranged by size:
5647 929 343
PmeI GTTT'AAAC
Cuts at: 4223 4223
Size: 6919
PmlI CAC'GTG
Cuts at: 2913 2913
Size: 6919
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4988 5020 6501 6533 4988
Size: 32 1481 32 5374
Fragments arranged by size:
5374 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6919
PsiI TTA'TAA
Cuts at: 4489 4489
Size: 6919
PspOMI G'GGCC_C
Cuts at: 2019 2019
Size: 6919
PstI C_TGCA'G
Cuts at: 830 2172 2368 3169 4271 830
Size: 1342 196 801 1102 3478
Fragments arranged by size:
3478 1342 1102 801 196
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6919
PvuII CAG'CTG
Cuts at: 4097 4097
Size: 6919
SacI G_AGCT'C
Cuts at: 2755 2755
Size: 6919
SalI G'TCGA_C
Cuts at: 1936 4261 1936
Size: 2325 4594
SapI GCTCTTCn'nnn_
Cuts at: 2117 2264 2117
Size: 147 6772
SbfI CC_TGCA'GG
Cuts at: 4271 4271
Size: 6919
ScaI AGT'ACT
Cuts at: 5167 6879 5167
Size: 1712 5207
SexAI A'CCwGG_T
Cuts at: 2373 2373
Size: 6919
SfcI C'TryA_G
Cuts at: 826 1045 2168 2364 3165 4267 5402 6083 6274
Size: 219 1123 196 801 1102 1135 681 191
Cuts at: 6274 826
Size: 1471
Fragments arranged by size:
1471 1135 1123 1102 801 681 219 196
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2027 2027
Size: 6919
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6919
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6919
SmaI CCC'GGG
Cuts at: 1872 4246 1872
Size: 2374 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2553 3111 5023 5924 6186
Size: 197 926 135 475 558 1912 901 262
Cuts at: 6186 820
Size: 1553
Fragments arranged by size:
1912 1553 926 901 558 475 262 197
135
SnaBI TAC'GTA
Cuts at: 493 4710 493
Size: 4217 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6919
SphI G_CATG'C
Cuts at: 1132 4277 1132
Size: 3145 3774
SspI AAT'ATT
Cuts at: 5 52 4843 5
Size: 47 4791 2081
Fragments arranged by size:
4791 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6919
StyI C'CwwG_G
Cuts at: 513 1065 2023 2434 2886 2940 3019 3797 3888
Size: 552 958 411 452 54 79 778 91
Cuts at: 3888 4358 513
Size: 470 3074
Fragments arranged by size:
3074 958 778 552 470 452 411 91
79 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2725 3677 4946 4963 5116 5301 5746
Size: 1255 1145 952 1269 17 153 185 445
Cuts at: 5746 325
Size: 1498
Fragments arranged by size:
1498 1269 1255 1145 952 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2330 2871 4071
Size: 274 80 33 51 1443 353 541 1200
Cuts at: 4071 4140 5165 6877 96
Size: 69 1025 1712 138
Fragments arranged by size:
1712 1443 1200 1025 541 353 274 138
80 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2700 2732 6879 6911 2700
Size: 32 4147 32 2708
Fragments arranged by size:
4147 2708 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4007 1316
Size: 2691 4228
XbaI T'CTAG_A
Cuts at: 4190 4255 4190
Size: 65 6854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2410 2677 2852 2989 2410
Size: 267 175 137 6340
Fragments arranged by size:
6340 267 175 137
XhoI C'TCGA_G
Cuts at: 1943 2553 1943
Size: 610 6309
XmaI C'CCGG_G
Cuts at: 1870 4244 1870
Size: 2374 4545
XmnI GAAnn'nnTTC
Cuts at: 5048 5048
Size: 6919
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3418 4727 276
Size: 53 83 186 936 1884 1309 2468
Fragments arranged by size:
2468 1884 1309 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI
PspXI PsrI RsrII SacII SanDI SfoI SrfI SwaI