Restriction map for
pFN21AE1311
(Circular) (Six-base) MAPSORT of: pFN21AE1311.seq Check: 8259 from: 1 to: 6112
pFN21AE1311 6112 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3198 3455 1937
Size: 1261 257 4594
Fragments arranged by size:
4594 1261 257
Acc65I G'GTAC_C
Cuts at: 3433 3433
Size: 6112
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3060 4175 5559 5827 1632
Size: 1428 1115 1384 268 1917
Fragments arranged by size:
1917 1428 1384 1115 268
AflII C'TTAA_G
Cuts at: 820 1017 2059 3056 820
Size: 197 1042 997 3876
Fragments arranged by size:
3876 1042 997 197
AflIII A'CryG_T
Cuts at: 1203 5011 5988 1203
Size: 3808 977 1327
Fragments arranged by size:
3808 1327 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6112
AlwNI CAG_nnn'CTG
Cuts at: 2029 2051 3855 5427 2029
Size: 22 1804 1572 2714
Fragments arranged by size:
2714 1804 1572 22
ApaLI G'TGCA_C
Cuts at: 4169 5325 4169
Size: 1156 4956
ApoI r'AATT_y
Cuts at: 1493 2820 3225 3237 3421 3647 3799 5908 1493
Size: 1327 405 12 184 226 152 2109 1697
Fragments arranged by size:
2109 1697 1327 405 226 184 152 12
AseI AT'TA_AT
Cuts at: 160 1032 2169 3249 4666 160
Size: 872 1137 1080 1417 1606
Fragments arranged by size:
1606 1417 1137 1080 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 2208 3437 1870
Size: 73 265 1229 4545
Fragments arranged by size:
4545 1229 265 73
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3433 4888 618
Size: 325 217 627 1646 1455 1842
Fragments arranged by size:
1842 1646 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2954 3431 1391
Size: 1563 477 4072
Fragments arranged by size:
4072 1563 477
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2507 3174 928
Size: 971 608 667 3866
Fragments arranged by size:
3866 971 667 608
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 1903
Size: 34 2366 34 3678
Fragments arranged by size:
3678 2366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2629 2755 4004 5214 2629
Size: 126 1249 1210 3527
Fragments arranged by size:
3527 1249 1210 126
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4723 136
Size: 107 122 71 631 605 3051 1525
Fragments arranged by size:
3051 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2318 2740 3337 6107 2318
Size: 422 597 2770 2323
Fragments arranged by size:
2770 2323 597 422
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 4173 5329 1790
Size: 2383 1156 2573
Fragments arranged by size:
2573 2383 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3843 4801 442
Size: 416 53 83 399 24 2426 958 1753
Fragments arranged by size:
2426 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2976 3048 3150 3302 4772 1239
Size: 1737 72 102 152 1470 2579
Fragments arranged by size:
2579 1737 1470 152 102 72
Bpu10I CC'TnA_GC
Cuts at: 2623 2623
Size: 6112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4237 5102 5400 4237
Size: 865 298 4949
Fragments arranged by size:
4949 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4775 882
Size: 617 3276 2219
Fragments arranged by size:
3276 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 2317 2577 6106 2317
Size: 260 3529 2323
Fragments arranged by size:
3529 2323 260
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2253 3919 4301 4936
Size: 53 83 186 936 720 1666 382 635
Cuts at: 4936 275
Size: 1451
Fragments arranged by size:
1666 1451 936 720 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2078 3241 4545 4943 5217 5364 5775 2078
Size: 1163 1304 398 274 147 411 2415
Fragments arranged by size:
2415 1304 1163 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2592 2622 1178
Size: 30 165 30 1189 30 4668
Fragments arranged by size:
4668 1189 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 1186
Size: 1234 4878
BseYI C'CCAG_C
Cuts at: 2096 5315 5811 2096
Size: 3219 496 2397
Fragments arranged by size:
3219 2397 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2376 2891 1163
Size: 573 640 515 4384
Fragments arranged by size:
4384 640 573 515
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2702 2776 4173 4258 5329 2702
Size: 74 1397 85 1071 3485
Fragments arranged by size:
3485 1397 1071 85 74
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2702 2776 2954 3431 4173 4258 5329
Size: 399 912 74 178 477 742 85 1071
Cuts at: 5329 1391
Size: 2174
Fragments arranged by size:
2174 1071 912 742 477 399 178 85
74
BspEI T'CCGG_A
Cuts at: 3241 5775 3241
Size: 2534 3578
BspHI T'CATG_A
Cuts at: 4001 5731 4001
Size: 1730 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3467 844
Size: 2623 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3286 3999 1394
Size: 429 1463 713 3507
Fragments arranged by size:
3507 1463 713 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3049 4607 4781 65
Size: 1183 1801 1558 174 1396
Fragments arranged by size:
1801 1558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4756 4943 1700
Size: 110 2946 187 2869
Fragments arranged by size:
2946 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 4172 5184 4172
Size: 1012 5100
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6112
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6112
BstYI r'GATC_y
Cuts at: 1972 2047 2318 2422 2740 3337 3442 3809 4194
Size: 75 271 104 318 597 105 367 385
Cuts at: 4194 4211 5663 5749 5761 6107 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 597 385 367 346 318 271
105 104 86 75 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4621
Fragments arranged by size:
4621 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5373
Fragments arranged by size:
5373 683 56
BtsI GCAGTG_nn'
Cuts at: 3628 4422 4442 3628
Size: 794 20 5298
Fragments arranged by size:
5298 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2691 2726 529
Size: 35 2127 35 3915
Fragments arranged by size:
3915 2127 35 35
DraI TTT'AAA
Cuts at: 2577 3416 2577
Size: 839 5273
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3559 4448 6077
Size: 54 1425 50 275 18 1729 889 1629
Cuts at: 6077 8
Size: 43
Fragments arranged by size:
1729 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4042 1353
Size: 2689 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4699 5073 5219 387
Size: 1064 138 3110 374 146 1280
Fragments arranged by size:
3110 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2976 3048 3060 3150 3302 4175 4772
Size: 393 1344 72 12 90 152 873 597
Cuts at: 4772 5559 5827 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1344 873 787 597 393 268 152
90 72 12
EcoRI G'AATT_C
Cuts at: 3421 3799 3421
Size: 378 5734
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 5259 1678
Size: 3581 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 2645 2677 4753
Size: 32 135 32 725 32 316 32 2076
Cuts at: 4753 4785 4827 4859 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2076 725 316 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3126 3456 3702 669
Size: 988 281 1188 330 246 3079
Fragments arranged by size:
3079 1188 988 330 281 246
HindIII A'AGCT_T
Cuts at: 748 2666 748
Size: 1918 4194
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KpnI G_GTAC'C
Cuts at: 3437 3437
Size: 6112
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2791 5226 5410 1305
Size: 1486 2435 184 2007
Fragments arranged by size:
2435 2007 1486 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4290
Fragments arranged by size:
4290 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4070 4429 4588 518
Size: 648 2904 359 159 2042
Fragments arranged by size:
2904 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3528 4205 5353 5598 1606
Size: 1922 677 1148 245 2120
Fragments arranged by size:
2120 1922 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4621
Fragments arranged by size:
4621 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 3470 5015 1132
Size: 97 2241 1545 2229
Fragments arranged by size:
2241 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6112
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6112
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3110 3682 3110
Size: 572 5540
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3054 3086 3054
Size: 32 6080
PstI C_TGCA'G
Cuts at: 830 2047 3464 830
Size: 1217 1417 3478
Fragments arranged by size:
3478 1417 1217
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SbfI CC_TGCA'GG
Cuts at: 3464 3464
Size: 6112
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SfcI C'TryA_G
Cuts at: 826 1045 2043 2466 2859 3195 3460 4595 5276
Size: 219 998 423 393 336 265 1135 681
Cuts at: 5276 5467 826
Size: 191 1471
Fragments arranged by size:
1471 1135 998 681 423 393 336 265
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 2210 3439 1872
Size: 338 1229 4545
Fragments arranged by size:
4545 1229 338
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2059 3056 4216 5117 5379 820
Size: 197 926 116 997 1160 901 262 1553
Fragments arranged by size:
1553 1160 997 926 901 262 197 116
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 3470 1132
Size: 2338 3774
SspI AAT'ATT
Cuts at: 5 52 2528 3017 4036 5
Size: 47 2476 489 1019 2081
Fragments arranged by size:
2476 2081 1019 489 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6112
StyI C'CwwG_G
Cuts at: 513 1065 2004 2175 3551 513
Size: 552 939 171 1376 3074
Fragments arranged by size:
3074 1376 939 552 171
SwaI ATTT'AAAT
Cuts at: 2577 2577
Size: 6112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2221 4139 4156 4309 4494 4939 325
Size: 1255 641 1918 17 153 185 445 1498
Fragments arranged by size:
1918 1498 1255 641 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3137 4358 6070
Size: 274 80 33 51 1443 1160 1221 1712
Cuts at: 6070 96
Size: 138
Fragments arranged by size:
1712 1443 1221 1160 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2259 2291 2592 2624 6072 6104 2259
Size: 32 301 32 3448 32 2267
Fragments arranged by size:
3448 2267 301 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6112
XbaI T'CTAG_A
Cuts at: 3448 3448
Size: 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 2208 3437 1870
Size: 338 1229 4545
Fragments arranged by size:
4545 1229 338
XmnI GAAnn'nnTTC
Cuts at: 3303 3319 4241 3303
Size: 16 922 5174
Fragments arranged by size:
5174 922 16
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI XcmI