Restriction map for
pFN21AE1309
(Circular) (Six-base) MAPSORT of: pFN21AE1309.seq Check: 9915 from: 1 to: 5776
pFN21AE1309 5776 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2376 3119 1937
Size: 439 743 4594
Fragments arranged by size:
4594 743 439
Acc65I G'GTAC_C
Cuts at: 2020 3097 2020
Size: 1077 4699
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3839 5223 5491 1632
Size: 2207 1384 268 1917
Fragments arranged by size:
2207 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 2721 4675 5652 1203
Size: 1518 1954 977 1327
Fragments arranged by size:
1954 1518 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 2150 2165 3519 5091 2150
Size: 15 1354 1572 2835
Fragments arranged by size:
2835 1572 1354 15
ApaI G_GGCC'C
Cuts at: 2259 2259
Size: 5776
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 2456 3085 3311 3463 5572 1493
Size: 963 629 226 152 2109 1697
Fragments arranged by size:
2109 1697 963 629 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2346 3101 1870
Size: 73 403 755 4545
Fragments arranged by size:
4545 755 403 73
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 3097 4552 618
Size: 325 217 627 233 1077 1455 1842
Fragments arranged by size:
1842 1455 1077 627 325 233 217
BanII G_rGCy'C
Cuts at: 1391 2259 2508 3095 1391
Size: 868 249 587 4072
Fragments arranged by size:
4072 868 587 249
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2771 928
Size: 971 872 3933
Fragments arranged by size:
3933 971 872
BbvCI CC'TCA_GC
Cuts at: 2197 2197
Size: 5776
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2324 2358 3967 4001 1903
Size: 34 387 34 1609 34 3678
Fragments arranged by size:
3678 1609 387 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 2757 1548
Size: 1209 4567
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 4387 136
Size: 107 122 71 631 605 343 2372 1525
Fragments arranged by size:
2372 1525 631 605 343 122 107 71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2259 3837 4993 1790
Size: 469 1578 1156 2573
Fragments arranged by size:
2573 1578 1156 469
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2048 2285 3507
Size: 416 53 83 399 24 631 237 1222
Cuts at: 3507 4465 442
Size: 958 1753
Fragments arranged by size:
1753 1222 958 631 416 399 237 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2115 2193 2606 4436 1239
Size: 876 78 413 1830 2579
Fragments arranged by size:
2579 1830 876 413 78
Bpu10I CC'TnA_GC
Cuts at: 2197 2197
Size: 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2785 3901 4766 5064 2785
Size: 1116 865 298 3497
Fragments arranged by size:
3497 1116 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2969 4439 882
Size: 617 1470 1470 2219
Fragments arranged by size:
2219 1470 1470 617
BsaAI yAC'GTr
Cuts at: 493 1204 3567 493
Size: 711 2363 2702
Fragments arranged by size:
2702 2363 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3583 3965 4600 275
Size: 53 83 186 936 2050 382 635 1451
Fragments arranged by size:
2050 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2113 4209 4607 4881 5028 5439 2113
Size: 2096 398 274 147 411 2450
Fragments arranged by size:
2450 2096 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2161 2191 1178
Size: 30 165 30 758 30 4763
Fragments arranged by size:
4763 758 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2025 1186
Size: 839 4937
BseYI C'CCAG_C
Cuts at: 2071 2221 4979 5475 2071
Size: 150 2758 496 2372
Fragments arranged by size:
2758 2372 496 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2270 1163
Size: 573 534 4669
Fragments arranged by size:
4669 573 534
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2272 2632 3226 3987 5015 1540
Size: 275 186 271 360 594 761 1028 2301
Fragments arranged by size:
2301 1028 761 594 360 275 271 186
BsiHKAI G_wGCw'C
Cuts at: 2035 2508 2550 3837 3922 4993 2035
Size: 473 42 1287 85 1071 2818
Fragments arranged by size:
2818 1287 1071 473 85 42
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
BsmBI CGTCTCn'nnnn_
Cuts at: 2320 2320
Size: 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2259 2508 2550 3095 3837 3922
Size: 399 245 224 249 42 545 742 85
Cuts at: 3922 4993 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 545 399 249 245 224
85 42
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 2760 3665 5395 2760
Size: 905 1730 3141
Fragments arranged by size:
3141 1730 905
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2777 3131 844
Size: 1933 354 3489
Fragments arranged by size:
3489 1933 354
BsrBI CCG'CTC
Cuts at: 1394 1823 2231 3663 1394
Size: 429 408 1432 3507
Fragments arranged by size:
3507 1432 429 408
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2101 4420 4607 1700
Size: 110 204 87 2319 187 2869
Fragments arranged by size:
2869 2319 204 187 110 87
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2105 2165 2554 794
Size: 1311 60 389 4016
Fragments arranged by size:
4016 1311 389 60
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 2395 3013 1184
Size: 1211 618 3947
Fragments arranged by size:
3947 1211 618
BstYI r'GATC_y
Cuts at: 1972 3106 3473 3858 3875 5327 5413 5425 5771
Size: 1134 367 385 17 1452 86 12 346
Cuts at: 5771 1972
Size: 1977
Fragments arranged by size:
1977 1452 1134 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2685 513
Size: 552 939 681 3604
Fragments arranged by size:
3604 939 681 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 3292 4086 4106 3292
Size: 794 20 4962
Fragments arranged by size:
4962 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DraIII CAC_nnn'GTG
Cuts at: 2389 2554 2389
Size: 165 5611
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2040 2182
Size: 54 1425 50 275 18 177 33 142
Cuts at: 2182 2269 2629 3024 3223 4112 5741 8
Size: 87 360 395 199 889 1629 43
Fragments arranged by size:
1629 1425 889 395 360 275 199 177
142 87 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2269 2629 3223 1537
Size: 275 457 360 594 4090
Fragments arranged by size:
4090 594 457 360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2253 3706 1353
Size: 900 1453 3423
Fragments arranged by size:
3423 1453 900
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2506 2506
Size: 5776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2193 2606 3839 4436 5223 5491
Size: 393 483 78 413 1233 597 787 268
Cuts at: 5491 1239
Size: 1524
Fragments arranged by size:
1524 1233 787 597 483 413 393 268
78
EcoO109I rG'GnC_Cy
Cuts at: 2255 2256 2358 2584 2255
Size: 1 102 226 5447
Fragments arranged by size:
5447 226 102 1
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 5776
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2090 2228 4923 1678
Size: 412 138 2695 2531
Fragments arranged by size:
2695 2531 412 138
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 2870 2902 4417
Size: 32 135 32 797 32 469 32 1515
Cuts at: 4417 4449 4491 4523 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1515 797 469 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2377 2449 2752 2941 3120 3366
Size: 988 281 439 72 303 189 179 246
Cuts at: 3366 669
Size: 3079
Fragments arranged by size:
3079 988 439 303 281 246 189 179
72
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 2941 3366 2941
Size: 425 5351
KpnI G_GTAC'C
Cuts at: 2024 3101 2024
Size: 1077 4699
MfeI C'AATT_G
Cuts at: 2401 3375 2401
Size: 974 4802
MluI A'CGCG_T
Cuts at: 2721 5652 2721
Size: 2931 2845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4890 5074 1305
Size: 3585 184 2007
Fragments arranged by size:
3585 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2184 3026 10
Size: 54 1425 343 177 175 842 2760
Fragments arranged by size:
2760 1425 842 343 177 175 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2663 2690 3734 4093 4252 518
Size: 648 1497 27 1044 359 159 2042
Fragments arranged by size:
2042 1497 1044 648 359 159 27
MspA1I CmG'CkG
Cuts at: 1606 2123 2162 2903 3192 3869 5017 5262 1606
Size: 517 39 741 289 677 1148 245 2120
Fragments arranged by size:
2120 1148 741 677 517 289 245 39
NcoI C'CATG_G
Cuts at: 513 1065 2004 2685 513
Size: 552 939 681 3604
Fragments arranged by size:
3604 939 681 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 2269 3223 2269
Size: 954 4822
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2675 1348
Size: 1327 4449
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5776
PfoI T'CCnGG_A
Cuts at: 2841 2841
Size: 5776
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2358 2358
Size: 5776
PshAI GACnn'nnGTC
Cuts at: 1613 2616 1613
Size: 1003 4773
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PspOMI G'GGCC_C
Cuts at: 2255 2255
Size: 5776
PstI C_TGCA'G
Cuts at: 830 3128 830
Size: 2298 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2903 2903
Size: 5776
SacI G_AGCT'C
Cuts at: 2508 2508
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 2375 3118 1936
Size: 439 743 4594
Fragments arranged by size:
4594 743 439
SanDI GG'GwC_CC
Cuts at: 2358 2358
Size: 5776
SapI GCTCTTCn'nnn_
Cuts at: 2253 2253
Size: 5776
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 2570 4024 5736 2570
Size: 1454 1712 2610
Fragments arranged by size:
2610 1712 1454
SexAI A'CCwGG_T
Cuts at: 3072 3072
Size: 5776
SfcI C'TryA_G
Cuts at: 826 1045 2572 3124 4259 4940 5131 826
Size: 219 1527 552 1135 681 191 1471
Fragments arranged by size:
1527 1471 1135 681 552 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 2014 1700
Size: 314 5462
SmaI CCC'GGG
Cuts at: 1872 3103 1872
Size: 1231 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2800 3880 4781 5043 820
Size: 197 926 857 1080 901 262 1553
Fragments arranged by size:
1553 1080 926 901 857 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2004 2685 3215 513
Size: 552 939 681 530 3074
Fragments arranged by size:
3074 939 681 552 530
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2304 3803 3820 3973 4158 4603 325
Size: 1255 724 1499 17 153 185 445 1498
Fragments arranged by size:
1499 1498 1255 724 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2568 2855 2881
Size: 274 80 33 51 1443 591 287 26
Cuts at: 2881 4022 5734 96
Size: 1141 1712 138
Fragments arranged by size:
1712 1443 1141 591 287 274 138 80
51 33 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 3101 1870
Size: 1231 4545
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI
SwaI XcmI