Restriction map for
pFN21AE1307
(Circular) (Six-base) MAPSORT of: pFN21AE1307.seq Check: 7932 from: 1 to: 6121
pFN21AE1307 6121 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2249 2249
Size: 6121
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2469 3931 278
Size: 53 83 186 936 933 1462 2468
Fragments arranged by size:
2468 1462 936 933 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2085 3291 3464 1937
Size: 148 1206 173 4594
Fragments arranged by size:
4594 1206 173 148
Acc65I G'GTAC_C
Cuts at: 2577 3442 2577
Size: 865 5256
AclI AA'CG_TT
Cuts at: 1563 4248 4621 1563
Size: 2685 373 3063
Fragments arranged by size:
3063 2685 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2228 2959 3332 4184 5568 5836 1632
Size: 596 731 373 852 1384 268 1917
Fragments arranged by size:
1917 1384 852 731 596 373 268
AfeI AGC'GCT
Cuts at: 2831 2831
Size: 6121
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5924
AflIII A'CryG_T
Cuts at: 1203 5020 5997 1203
Size: 3817 977 1327
Fragments arranged by size:
3817 1327 977
AgeI A'CCGG_T
Cuts at: 4952 4952
Size: 6121
AhdI GACnn_n'nnGTC
Cuts at: 2158 2594 4850 2158
Size: 436 2256 3429
Fragments arranged by size:
3429 2256 436
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3002 1184
Size: 1818 4303
AlwNI CAG_nnn'CTG
Cuts at: 2117 2250 2424 2728 2786 2982 3228 3864 5436
Size: 133 174 304 58 196 246 636 1572
Cuts at: 5436 2117
Size: 2802
Fragments arranged by size:
2802 1572 636 304 246 196 174 133
58
ApaI G_GGCC'C
Cuts at: 2350 2350
Size: 6121
ApaLI G'TGCA_C
Cuts at: 4178 5334 4178
Size: 1156 4965
ApoI r'AATT_y
Cuts at: 1493 3430 3656 3808 5917 1493
Size: 1937 226 152 2109 1697
Fragments arranged by size:
2109 1937 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4675 160
Size: 872 3643 1606
Fragments arranged by size:
3643 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
AvaI C'yCGr_G
Cuts at: 1870 1943 2343 3446 1870
Size: 73 400 1103 4545
Fragments arranged by size:
4545 1103 400 73
BamHI G'GATC_C
Cuts at: 3451 3818 3451
Size: 367 5754
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2577 2881 3442 4897 618
Size: 325 217 627 790 304 561 1455 1842
Fragments arranged by size:
1842 1455 790 627 561 325 304 217
BanII G_rGCy'C
Cuts at: 1391 2336 2343 2350 2376 3129 3354 3381 3440
Size: 945 7 7 26 753 225 27 59
Cuts at: 3440 1391
Size: 4072
Fragments arranged by size:
4072 945 753 225 59 27 26 7
7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2236 2270 4312 4346 1903
Size: 34 299 34 2042 34 3678
Fragments arranged by size:
3678 2042 299 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4013 5223 4013
Size: 1210 4911
BclI T'GATC_A
Cuts at: 1548 2448 2601 2931 1548
Size: 900 153 330 4738
Fragments arranged by size:
4738 900 330 153
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4732 136
Size: 107 122 71 631 605 3060 1525
Fragments arranged by size:
3060 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6116 6116
Size: 6121
BlpI GC'TnA_GC
Cuts at: 3179 3538 3179
Size: 359 5762
Bme1580I G_kGCm'C
Cuts at: 1790 2350 2886 4182 5338 1790
Size: 560 536 1296 1156 2573
Fragments arranged by size:
2573 1296 1156 560 536
BmgBI CAC'GTC
Cuts at: 1318 2329 3213 1318
Size: 1011 884 4226
Fragments arranged by size:
4226 1011 884
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2161 2289 3852
Size: 416 53 83 399 24 744 128 1563
Cuts at: 3852 4810 442
Size: 958 1753
Fragments arranged by size:
1753 1563 958 744 416 399 128 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 3116 3177 4781 1239
Size: 833 1044 61 1604 2579
Fragments arranged by size:
2579 1604 1044 833 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4246 5111 5409 4246
Size: 865 298 4958
Fragments arranged by size:
4958 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2533 3022 4784 882
Size: 617 1034 489 1762 2219
Fragments arranged by size:
2219 1762 1034 617 489
BsaAI yAC'GTr
Cuts at: 493 1204 3912 493
Size: 711 2708 2702
Fragments arranged by size:
2708 2702 711
BsaBI GATnn'nnATC
Cuts at: 6115 6115
Size: 6121
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2466 3928 4310 4945
Size: 53 83 186 936 933 1462 382 635
Cuts at: 4945 275
Size: 1451
Fragments arranged by size:
1462 1451 936 933 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4554 4952 5226 5373 5784 4554
Size: 398 274 147 411 4891
Fragments arranged by size:
4891 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3069 3099 1178
Size: 30 165 30 1666 30 4200
Fragments arranged by size:
4200 1666 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2296 1186
Size: 1110 5011
BseYI C'CCAG_C
Cuts at: 3117 3361 5324 5820 3117
Size: 244 1963 496 3418
Fragments arranged by size:
3418 1963 496 244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 2538 2741 2756 1163
Size: 573 606 196 203 15 4528
Fragments arranged by size:
4528 606 573 203 196 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2962 3571 4332 5360 1540
Size: 275 186 961 609 761 1028 2301
Fragments arranged by size:
2301 1028 961 761 609 275 186
BsiHKAI G_wGCw'C
Cuts at: 3381 4182 4267 5338 3381
Size: 801 85 1071 4164
Fragments arranged by size:
4164 1071 801 85
BsmI GAATG_Cn'
Cuts at: 3632 3725 3632
Size: 93 6028
BsmBI CGTCTCn'nnnn_
Cuts at: 2457 2504 2457
Size: 47 6074
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2336 2343 2350 2376 2886 3129 3354
Size: 399 546 7 7 26 510 243 225
Cuts at: 3354 3381 3440 4182 4267 5338 1391
Size: 27 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 546 510 399 243 225
85 59 27 26 7 7
BspEI T'CCGG_A
Cuts at: 5784 5784
Size: 6121
BspHI T'CATG_A
Cuts at: 2598 4010 5740 2598
Size: 1412 1730 2979
Fragments arranged by size:
2979 1730 1412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 2249 3271 3476 844
Size: 1253 152 1022 205 3489
Fragments arranged by size:
3489 1253 1022 205 152
BsrBI CCG'CTC
Cuts at: 1394 1823 4008 1394
Size: 429 2185 3507
Fragments arranged by size:
3507 2185 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2482 4616 4790 65
Size: 1183 1234 2134 174 1396
Fragments arranged by size:
2134 1396 1234 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2187 4765 4952 1700
Size: 110 377 2578 187 2869
Fragments arranged by size:
2869 2578 377 187 110
BsrGI T'GTAC_A
Cuts at: 96 3219 96
Size: 3123 2998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6121
BssSI C'ACGA_G
Cuts at: 4181 5193 4181
Size: 1012 5109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2250 794
Size: 1456 4665
BstBI TT'CG_AA
Cuts at: 4925 4925
Size: 6121
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6121
BstXI CCAn_nnnn'nTGG
Cuts at: 2050 3331 2050
Size: 1281 4840
BstYI r'GATC_y
Cuts at: 1972 2760 3451 3818 4203 4220 5672 5758 5770
Size: 788 691 367 385 17 1452 86 12
Cuts at: 5770 6116 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 788 691 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2999 2999
Size: 6121
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4630
Fragments arranged by size:
4630 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5382
Fragments arranged by size:
5382 683 56
BtsI GCAGTG_nn'
Cuts at: 2422 3637 4431 4451 2422
Size: 1215 794 20 4092
Fragments arranged by size:
4092 1215 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6121
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6086
DraI TTT'AAA
Cuts at: 3425 3425
Size: 6121
DraIII CAC_nnn'GTG
Cuts at: 2613 2613
Size: 6121
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5128 809
Size: 507 3812 1802
Fragments arranged by size:
3812 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2136 2430 3568
Size: 54 1425 50 275 18 306 294 1138
Cuts at: 3568 4457 6086 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1138 889 306 294 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3568 1537
Size: 275 1756 4090
Fragments arranged by size:
4090 1756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 3056 4051 1353
Size: 717 986 995 3423
Fragments arranged by size:
3423 995 986 717
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4708 5082 5228 387
Size: 1064 138 3119 374 146 1280
Fragments arranged by size:
3119 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3379 3379
Size: 6121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2228 2959 3116 3177 3332 4184
Size: 393 440 156 731 157 61 155 852
Cuts at: 4184 4781 5568 5836 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 852 787 731 597 440 393 268
157 156 155 61
EcoO109I rG'GnC_Cy
Cuts at: 2066 2122 2347 2995 3080 3161 2066
Size: 56 225 648 85 81 5026
Fragments arranged by size:
5026 648 225 85 81 56
EcoRI G'AATT_C
Cuts at: 3430 3808 3430
Size: 378 5743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2762 2762
Size: 6121
FspI TGC'GCA
Cuts at: 2809 4627 2809
Size: 1818 4303
HaeII r_GCGC'y
Cuts at: 1678 2710 2833 3199 5268 1678
Size: 1032 123 366 2069 2531
Fragments arranged by size:
2531 2069 1032 366 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2144 2176 2318 2350 2580
Size: 32 135 32 572 32 142 32 230
Cuts at: 2580 2612 2716 2748 3021 3053 3389 3421 4762
Size: 32 104 32 273 32 336 32 1341
Cuts at: 4762 4794 4836 4868 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1341 572 336 273 230 142 135
104 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2152 3465 3711 669
Size: 988 281 214 1313 246 3079
Fragments arranged by size:
3079 1313 988 281 246 214
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6121
HpaI GTT'AAC
Cuts at: 3711 3711
Size: 6121
KpnI G_GTAC'C
Cuts at: 2581 3446 2581
Size: 865 5256
MfeI C'AATT_G
Cuts at: 3720 3720
Size: 6121
MluI A'CGCG_T
Cuts at: 5997 5997
Size: 6121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5235 5419 1305
Size: 3930 184 2007
Fragments arranged by size:
3930 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2138 2432 10
Size: 54 1425 343 306 294 3699
Fragments arranged by size:
3699 1425 343 306 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2318 2688 4079 4438 4597 518
Size: 648 1152 370 1391 359 159 2042
Fragments arranged by size:
2042 1391 1152 648 370 359 159
MspA1I CmG'CkG
Cuts at: 1606 2250 2436 2654 3537 4214 5362 5607 1606
Size: 644 186 218 883 677 1148 245 2120
Fragments arranged by size:
2120 1148 883 677 644 245 218 186
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4630
Fragments arranged by size:
4630 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6121
NotI GC'GGCC_GC
Cuts at: 3568 3568
Size: 6121
NspI r_CATG'y
Cuts at: 1132 1229 3479 5024 1132
Size: 97 2250 1545 2229
Fragments arranged by size:
2250 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2070 2458 3389 1348
Size: 722 388 931 4080
Fragments arranged by size:
4080 931 722 388
PciI A'CATG_T
Cuts at: 5020 5020
Size: 6121
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2428 1108
Size: 1320 4801
PmeI GTTT'AAAC
Cuts at: 3425 3425
Size: 6121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4190 4222 5703 5735 4190
Size: 32 1481 32 4576
Fragments arranged by size:
4576 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 2995 2066
Size: 929 5192
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6121
PsiI TTA'TAA
Cuts at: 3691 3691
Size: 6121
PspOMI G'GGCC_C
Cuts at: 2346 2346
Size: 6121
PstI C_TGCA'G
Cuts at: 830 2246 3282 3473 830
Size: 1416 1036 191 3478
Fragments arranged by size:
3478 1416 1036 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6121
PvuII CAG'CTG
Cuts at: 2250 2250
Size: 6121
SacI G_AGCT'C
Cuts at: 3381 3381
Size: 6121
SalI G'TCGA_C
Cuts at: 1936 3463 1936
Size: 1527 4594
SapI GCTCTTCn'nnn_
Cuts at: 3056 3056
Size: 6121
SbfI CC_TGCA'GG
Cuts at: 3473 3473
Size: 6121
ScaI AGT'ACT
Cuts at: 4369 6081 4369
Size: 1712 4409
SfcI C'TryA_G
Cuts at: 826 1045 2242 3278 3469 4604 5285 5476 826
Size: 219 1197 1036 191 1135 681 191 1471
Fragments arranged by size:
1471 1197 1135 1036 681 219 191 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6121
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6121
SmaI CCC'GGG
Cuts at: 1872 2345 3448 1872
Size: 473 1103 4545
Fragments arranged by size:
4545 1103 473
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4225 5126 5388 820
Size: 197 926 2282 901 262 1553
Fragments arranged by size:
2282 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3912 493
Size: 3419 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6121
SphI G_CATG'C
Cuts at: 1132 3479 1132
Size: 2347 3774
SspI AAT'ATT
Cuts at: 5 52 4045 5
Size: 47 3993 2081
Fragments arranged by size:
3993 2081 47
StuI AGG'CCT
Cuts at: 1517 2928 1517
Size: 1411 4710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2376 2754 2791 3355 3560 513
Size: 552 939 372 378 37 564 205 3074
Fragments arranged by size:
3074 939 564 552 378 372 205 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4148 4165 4318 4503 4948 325
Size: 1255 2568 17 153 185 445 1498
Fragments arranged by size:
2568 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3219 4367 6079
Size: 274 80 33 51 1443 1242 1148 1712
Cuts at: 6079 96
Size: 138
Fragments arranged by size:
1712 1443 1242 1148 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3069 3101 3135 3167 6081 6113 3069
Size: 32 34 32 2914 32 3077
Fragments arranged by size:
3077 2914 34 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2728 1316
Size: 1412 4709
XbaI T'CTAG_A
Cuts at: 3457 3457
Size: 6121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 2425 3331 2398
Size: 27 906 5188
Fragments arranged by size:
5188 906 27
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6121
XmaI C'CCGG_G
Cuts at: 1870 2343 3446 1870
Size: 473 1103 4545
Fragments arranged by size:
4545 1103 473
XmnI GAAnn'nnTTC
Cuts at: 4250 4250
Size: 6121
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2467 3929 276
Size: 53 83 186 936 933 1462 2468
Fragments arranged by size:
2468 1462 936 933 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI