Restriction map for
pFN21AE1303
(Circular) (Six-base) MAPSORT of: pFN21AE1303.seq Check: 4989 from: 1 to: 5953
pFN21AE1303 5953 bp
With 182 enzymes: SgfI *
May 22, 2017 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2056 2742 2056
Size: 686 5267
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3763 278
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2754 3296 1937
Size: 817 542 4594
Fragments arranged by size:
4594 817 542
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 4080 4453 1563
Size: 2517 373 3063
Fragments arranged by size:
3063 2517 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2934 3156 4016 5400 5668 1632
Size: 1302 222 860 1384 268 1917
Fragments arranged by size:
1917 1384 1302 860 268 222
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5756
AflIII A'CryG_T
Cuts at: 1203 4852 5829 1203
Size: 3649 977 1327
Fragments arranged by size:
3649 1327 977
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 3145 4682 3145
Size: 1537 4416
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5953
AlwNI CAG_nnn'CTG
Cuts at: 2147 2750 3696 5268 2147
Size: 603 946 1572 2832
Fragments arranged by size:
2832 1572 946 603
ApaLI G'TGCA_C
Cuts at: 4010 5166 4010
Size: 1156 4797
ApoI r'AATT_y
Cuts at: 1493 3262 3488 3640 5749 1493
Size: 1769 226 152 2109 1697
Fragments arranged by size:
2109 1769 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2939 3278 1870
Size: 73 253 743 339 4545
Fragments arranged by size:
4545 743 339 253 73
BamHI G'GATC_C
Cuts at: 3283 3650 3283
Size: 367 5586
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 3274 4729 618
Size: 325 217 627 250 1237 1455 1842
Fragments arranged by size:
1842 1455 1237 627 325 250 217
BanII G_rGCy'C
Cuts at: 1391 2481 2535 3130 3272 1391
Size: 1090 54 595 142 4072
Fragments arranged by size:
4072 1090 595 142 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2922 3144 928
Size: 971 1023 222 3737
Fragments arranged by size:
3737 1023 971 222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2280 2314 2609 2643 2930 2964 4144
Size: 34 343 34 295 34 287 34 1180
Cuts at: 4144 4178 1903
Size: 34 3678
Fragments arranged by size:
3678 1180 343 295 287 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 2178 1548
Size: 630 5323
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4564 136
Size: 107 122 71 631 605 2892 1525
Fragments arranged by size:
2892 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2185 5948 2185
Size: 3763 2190
BlpI GC'TnA_GC
Cuts at: 2093 3370 2093
Size: 1277 4676
Bme1580I G_kGCm'C
Cuts at: 1790 4014 5170 1790
Size: 2224 1156 2573
Fragments arranged by size:
2573 2224 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5953
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2321 3012 3684
Size: 416 53 83 399 24 904 691 672
Cuts at: 3684 4642 442
Size: 958 1753
Fragments arranged by size:
1753 958 904 691 672 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5953
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2226 2917 2226
Size: 691 5262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2424 2675 3085 4613 1239
Size: 971 214 251 410 1528 2579
Fragments arranged by size:
2579 1528 971 410 251 214
Bpu10I CC'TnA_GC
Cuts at: 2468 3158 2468
Size: 690 5263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2450 4078 4943 5241 2450
Size: 1628 865 298 3162
Fragments arranged by size:
3162 1628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3092 4616 882
Size: 617 1593 1524 2219
Fragments arranged by size:
2219 1593 1524 617
BsaAI yAC'GTr
Cuts at: 493 1204 3744 493
Size: 711 2540 2702
Fragments arranged by size:
2702 2540 711
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2578 2994 3760
Size: 53 83 186 936 552 493 416 766
Cuts at: 3760 4142 4777 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 766 635 552 493 416 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2371 3225 4386 4784 5058 5205 5616 2371
Size: 854 1161 398 274 147 411 2708
Fragments arranged by size:
2708 1161 854 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5728
Fragments arranged by size:
5728 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2578 1186
Size: 1392 4561
BseYI C'CCAG_C
Cuts at: 2822 5156 5652 2822
Size: 2334 496 3123
Fragments arranged by size:
3123 2334 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5380
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2784 3403 4164 5192 1540
Size: 275 186 783 619 761 1028 2301
Fragments arranged by size:
2301 1028 783 761 619 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 2428 2683 4014 4099 5170 2337
Size: 91 255 1331 85 1071 3120
Fragments arranged by size:
3120 1331 1071 255 91 85
BsmI GAATG_Cn'
Cuts at: 3464 3557 3464
Size: 93 5860
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 2428 2481 2535 2683 3130 3272
Size: 399 547 91 53 54 148 447 142
Cuts at: 3272 4014 4099 5170 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 547 447 399 148 142
91 85 54 53
BspEI T'CCGG_A
Cuts at: 3225 5616 3225
Size: 2391 3562
BspHI T'CATG_A
Cuts at: 3842 5572 3842
Size: 1730 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2056 2742 3150 3308 844
Size: 1212 686 408 158 3489
Fragments arranged by size:
3489 1212 686 408 158
BsrBI CCG'CTC
Cuts at: 1394 1823 3840 1394
Size: 429 2017 3507
Fragments arranged by size:
3507 2017 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4448 4622 65
Size: 1183 3200 174 1396
Fragments arranged by size:
3200 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 4597 4784 1700
Size: 110 230 2557 187 2869
Fragments arranged by size:
2869 2557 230 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 4013 5025 4013
Size: 1012 4941
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5953
BstBI TT'CG_AA
Cuts at: 4757 4757
Size: 5953
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5953
BstXI CCAn_nnnn'nTGG
Cuts at: 2389 2638 2864 2389
Size: 249 226 5478
Fragments arranged by size:
5478 249 226
BstYI r'GATC_y
Cuts at: 1972 2150 2185 3283 3650 4035 4052 5504 5590
Size: 178 35 1098 367 385 17 1452 86
Cuts at: 5590 5602 5948 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1098 385 367 346 178 86
35 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2674 2884 513
Size: 552 939 670 210 3582
Fragments arranged by size:
3582 939 670 552 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5214
Fragments arranged by size:
5214 683 56
BtsI GCAGTG_nn'
Cuts at: 2488 3128 3469 4263 4283 2488
Size: 640 341 794 20 4158
Fragments arranged by size:
4158 794 640 341 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5918
DraI TTT'AAA
Cuts at: 3244 3257 3244
Size: 13 5940
DraIII CAC_nnn'GTG
Cuts at: 3020 3020
Size: 5953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2485 3400 4289
Size: 54 1425 50 275 18 655 915 889
Cuts at: 4289 5918 8
Size: 1629 43
Fragments arranged by size:
1629 1425 915 889 655 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2217 3883 1353
Size: 699 165 1666 3423
Fragments arranged by size:
3423 1666 699 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4540 4914 5060 387
Size: 1064 138 2951 374 146 1280
Fragments arranged by size:
2951 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2424 2675 2934 3085 3156 4016
Size: 393 578 214 251 259 151 71 860
Cuts at: 4016 4613 5400 5668 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 860 787 597 578 393 268 259
251 214 151 71
EcoO109I rG'GnC_Cy
Cuts at: 2112 2976 3070 2112
Size: 864 94 4995
Fragments arranged by size:
4995 864 94
EcoRI G'AATT_C
Cuts at: 3262 3640 3262
Size: 378 5575
FspI TGC'GCA
Cuts at: 4459 4459
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 5100 1678
Size: 3422 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2170 2202 2984 3016 3131
Size: 32 135 32 598 32 782 32 115
Cuts at: 3131 3163 4594 4626 4668 4700 1373
Size: 32 1431 32 42 32 2626
Fragments arranged by size:
2626 1431 782 598 135 115 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3297 3543 669
Size: 988 281 1359 246 3079
Fragments arranged by size:
3079 1359 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5953
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 3552 3552
Size: 5953
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2577 5067 5251 1305
Size: 1272 2490 184 2007
Fragments arranged by size:
2490 2007 1272 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2487 10
Size: 54 1425 343 655 3476
Fragments arranged by size:
3476 1425 655 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3029 3911 4270 4429 518
Size: 648 1863 882 359 159 2042
Fragments arranged by size:
2042 1863 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2176 2817 2989 3369 4046 5194 5439
Size: 531 39 641 172 380 677 1148 245
Cuts at: 5439 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 641 531 380 245 172
39
NaeI GCC'GGC
Cuts at: 2042 2042
Size: 5953
NcoI C'CATG_G
Cuts at: 513 1065 2004 2884 513
Size: 552 939 880 3582
Fragments arranged by size:
3582 939 880 552
NdeI CA'TA_TG
Cuts at: 387 2118 387
Size: 1731 4222
NgoMIV G'CCGG_C
Cuts at: 2040 2040
Size: 5953
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5953
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NspI r_CATG'y
Cuts at: 1132 1229 2257 3311 4856 1132
Size: 97 1028 1054 1545 2229
Fragments arranged by size:
2229 1545 1054 1028 97
PasI CC'CwG_GG
Cuts at: 1348 2744 1348
Size: 1396 4557
PciI A'CATG_T
Cuts at: 4852 4852
Size: 5953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5953
PfoI T'CCnGG_A
Cuts at: 2961 3062 2961
Size: 101 5852
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 5535 5567 4022
Size: 32 1481 32 4408
Fragments arranged by size:
4408 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2976 3070 2976
Size: 94 5859
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 3195 3523 3195
Size: 328 5625
PspXI vC'TCGA_Gb
Cuts at: 2196 2196
Size: 5953
PstI C_TGCA'G
Cuts at: 830 2067 2739 3147 3305 830
Size: 1237 672 408 158 3478
Fragments arranged by size:
3478 1237 672 408 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
PvuII CAG'CTG
Cuts at: 2176 2817 2989 2176
Size: 641 172 5140
Fragments arranged by size:
5140 641 172
SalI G'TCGA_C
Cuts at: 1936 3295 1936
Size: 1359 4594
SanDI GG'GwC_CC
Cuts at: 3070 3070
Size: 5953
SapI GCTCTTCn'nnn_
Cuts at: 2052 2217 2052
Size: 165 5788
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 4201 5913 4201
Size: 1712 4241
SfcI C'TryA_G
Cuts at: 826 1045 2063 2735 3143 3301 4436 5117 5308
Size: 219 1018 672 408 158 1135 681 191
Cuts at: 5308 826
Size: 1471
Fragments arranged by size:
1471 1135 1018 681 672 408 219 191
158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5953
SmaI CCC'GGG
Cuts at: 1872 3280 1872
Size: 1408 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2196 2429 4057 4958 5220 820
Size: 197 926 253 233 1628 901 262 1553
Fragments arranged by size:
1628 1553 926 901 262 253 233 197
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 2861 1517
Size: 1344 4609
StyI C'CwwG_G
Cuts at: 513 1065 2004 2884 3392 513
Size: 552 939 880 508 3074
Fragments arranged by size:
3074 939 880 552 508
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2395 2770 3031 3980 3997 4150 4335
Size: 1255 815 375 261 949 17 153 185
Cuts at: 4335 4780 325
Size: 445 1498
Fragments arranged by size:
1498 1255 949 815 445 375 261 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4199 5911 96
Size: 274 80 33 51 1443 2222 1712 138
Fragments arranged by size:
2222 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2760 2792 5913 5945 2760
Size: 32 3121 32 2768
Fragments arranged by size:
3121 2768 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5953
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2864 2864
Size: 5953
XhoI C'TCGA_G
Cuts at: 1943 2196 1943
Size: 253 5700
XmaI C'CCGG_G
Cuts at: 1870 3278 1870
Size: 1408 4545
XmnI GAAnn'nnTTC
Cuts at: 2801 4082 2801
Size: 1281 4672
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3761 276
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PsrI RsrII SacI SacII SexAI SfiI
SfoI SrfI SwaI