Restriction map for
pFN21AE1300
(Circular) (Six-base) MAPSORT of: pFN21AE1300.seq Check: 4997 from: 1 to: 6421
pFN21AE1300 6421 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2079 4231 278
Size: 53 83 186 936 543 2152 2468
Fragments arranged by size:
2468 2152 936 543 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3764 1937
Size: 1827 4594
Acc65I G'GTAC_C
Cuts at: 2633 3742 2633
Size: 1109 5312
AclI AA'CG_TT
Cuts at: 1563 4548 4921 1563
Size: 2985 373 3063
Fragments arranged by size:
3063 2985 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3180 3342 4484 5868 6136 1632
Size: 1548 162 1142 1384 268 1917
Fragments arranged by size:
1917 1548 1384 1142 268 162
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6224
AflIII A'CryG_T
Cuts at: 1203 2025 5320 6297 1203
Size: 822 3295 977 1327
Fragments arranged by size:
3295 1327 977 822
AgeI A'CCGG_T
Cuts at: 5252 5252
Size: 6421
AhdI GACnn_n'nnGTC
Cuts at: 5150 5150
Size: 6421
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6421
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6421
AlwNI CAG_nnn'CTG
Cuts at: 2143 4164 5736 2143
Size: 2021 1572 2828
Fragments arranged by size:
2828 2021 1572
ApaI G_GGCC'C
Cuts at: 2669 2927 3395 2669
Size: 258 468 5695
Fragments arranged by size:
5695 468 258
ApaLI G'TGCA_C
Cuts at: 2207 4478 5634 2207
Size: 2271 1156 2994
Fragments arranged by size:
2994 2271 1156
ApoI r'AATT_y
Cuts at: 1493 3730 3956 4108 6217 1493
Size: 2237 226 152 2109 1697
Fragments arranged by size:
2237 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4975 160
Size: 872 3943 1606
Fragments arranged by size:
3943 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
AvaI C'yCGr_G
Cuts at: 1870 1943 2110 2158 2303 2326 3698 3746 1870
Size: 73 167 48 145 23 1372 48 4545
Fragments arranged by size:
4545 1372 167 145 73 48 48 23
BamHI G'GATC_C
Cuts at: 3751 4118 3751
Size: 367 6054
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2406 2543 2633 2650 3564
Size: 325 217 627 619 137 90 17 914
Cuts at: 3564 3742 5197 618
Size: 178 1455 1842
Fragments arranged by size:
1842 1455 914 627 619 325 217 178
137 90 17
BanII G_rGCy'C
Cuts at: 1391 2157 2669 2738 2859 2888 2927 3017 3072
Size: 766 512 69 121 29 39 90 55
Cuts at: 3072 3395 3624 3740 1391
Size: 323 229 116 4072
Fragments arranged by size:
4072 766 512 323 229 121 116 90
69 55 39 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3430 3585 3658 928
Size: 971 1531 155 73 3691
Fragments arranged by size:
3691 1531 971 155 73
BbvCI CC'TCA_GC
Cuts at: 2345 2661 3150 3533 2345
Size: 316 489 383 5233
Fragments arranged by size:
5233 489 383 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4612 4646 1903
Size: 34 2675 34 3678
Fragments arranged by size:
3678 2675 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4313 5523 4313
Size: 1210 5211
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6421
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2124 2832 2904
Size: 107 122 71 631 605 452 708 72
Cuts at: 2904 3489 5032 136
Size: 585 1543 1525
Fragments arranged by size:
1543 1525 708 631 605 585 452 122
107 72 71
BglII A'GATC_T
Cuts at: 6416 6416
Size: 6421
BlpI GC'TnA_GC
Cuts at: 3838 3838
Size: 6421
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2297 2655 2669 2927 3395 4482 5638
Size: 421 86 358 14 258 468 1087 1156
Cuts at: 5638 1790
Size: 2573
Fragments arranged by size:
2573 1156 1087 468 421 358 258 86
14
BmgBI CAC'GTC
Cuts at: 1318 2212 1318
Size: 894 5527
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3194 4152 5110
Size: 416 53 83 399 24 1777 958 958
Cuts at: 5110 442
Size: 1753
Fragments arranged by size:
1777 1753 958 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6421
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2825
Size: 6421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2150 2450 2509 2536 3395 5081 1239
Size: 911 300 59 27 859 1686 2579
Fragments arranged by size:
2579 1686 911 859 300 59 27
Bpu10I CC'TnA_GC
Cuts at: 2094 2345 2661 3150 3533 2094
Size: 251 316 489 383 4982
Fragments arranged by size:
4982 489 383 316 251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2843 4546 5411 5709 2843
Size: 1703 865 298 3555
Fragments arranged by size:
3555 1703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2796 3218 5084 882
Size: 617 1297 422 1866 2219
Fragments arranged by size:
2219 1866 1297 617 422
BsaAI yAC'GTr
Cuts at: 493 1204 4212 493
Size: 711 3008 2702
Fragments arranged by size:
3008 2702 711
BsaBI GATnn'nnATC
Cuts at: 2267 6415 2267
Size: 4148 2273
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 3213 3366 4228
Size: 53 83 186 936 543 1137 153 862
Cuts at: 4228 4610 5245 275
Size: 382 635 1451
Fragments arranged by size:
1451 1137 936 862 635 543 382 186
153 83 53
BsaWI w'CCGG_w
Cuts at: 4854 5252 5526 5673 6084 4854
Size: 398 274 147 411 5191
Fragments arranged by size:
5191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6196
Fragments arranged by size:
6196 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2819 3022 1186
Size: 1633 203 4585
Fragments arranged by size:
4585 1633 203
BseYI C'CCAG_C
Cuts at: 2015 2398 2449 2919 5624 6120 2015
Size: 383 51 470 2705 496 2316
Fragments arranged by size:
2705 2316 496 470 383 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2305 1163
Size: 573 569 5279
Fragments arranged by size:
5279 573 569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3871 4632 5660 1540
Size: 275 186 1870 761 1028 2301
Fragments arranged by size:
2301 1870 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2211 2859 4482 4567 5638 2100
Size: 111 648 1623 85 1071 2883
Fragments arranged by size:
2883 1623 1071 648 111 85
BsmI GAATG_Cn'
Cuts at: 3932 4025 3932
Size: 93 6328
BsmBI CGTCTCn'nnnn_
Cuts at: 2554 2554
Size: 6421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2157 2211 2297 2655 2669 2738
Size: 399 310 57 54 86 358 14 69
Cuts at: 2738 2859 2888 2927 3017 3072 3395 3624 3740
Size: 121 29 39 90 55 323 229 116
Cuts at: 3740 4482 4567 5638 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 323 310 229
121 116 90 86 85 69 57 55
54 39 29 14
BspEI T'CCGG_A
Cuts at: 6084 6084
Size: 6421
BspHI T'CATG_A
Cuts at: 4310 6040 4310
Size: 1730 4691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2151 3776 844
Size: 1307 1625 3489
Fragments arranged by size:
3489 1625 1307
BsrBI CCG'CTC
Cuts at: 1394 1823 4308 1394
Size: 429 2485 3507
Fragments arranged by size:
3507 2485 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2647 3499 4916 5090 65
Size: 1183 1399 852 1417 174 1396
Fragments arranged by size:
1417 1399 1396 1183 852 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5065 5252 1700
Size: 110 3255 187 2869
Fragments arranged by size:
3255 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6421
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6421
BssSI C'ACGA_G
Cuts at: 4481 5493 4481
Size: 1012 5409
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2292 2368 3498 794
Size: 1498 76 1130 3717
Fragments arranged by size:
3717 1498 1130 76
BstBI TT'CG_AA
Cuts at: 5225 5225
Size: 6421
BstEII G'GTnAC_C
Cuts at: 1184 3349 1184
Size: 2165 4256
BstXI CCAn_nnnn'nTGG
Cuts at: 3494 3494
Size: 6421
BstYI r'GATC_y
Cuts at: 1972 3639 3702 3751 4118 4503 4520 5972 6058
Size: 1667 63 49 367 385 17 1452 86
Cuts at: 6058 6070 6416 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1667 1452 385 367 346 86 63
49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2523 2815 3354 513
Size: 552 939 48 471 292 539 3580
Fragments arranged by size:
3580 939 552 539 471 292 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2593 2641 487
Size: 683 56 1367 48 4267
Fragments arranged by size:
4267 1367 683 56 48
BtsI GCAGTG_nn'
Cuts at: 3937 4731 4751 3937
Size: 794 20 5607
Fragments arranged by size:
5607 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3217 3252 3445 3480 529
Size: 35 2653 35 193 35 3470
Fragments arranged by size:
3470 2653 193 35 35 35
DraI TTT'AAA
Cuts at: 3725 3725
Size: 6421
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5428 809
Size: 507 4112 1802
Fragments arranged by size:
4112 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2055 2728
Size: 54 1425 50 275 18 219 6 673
Cuts at: 2728 2763 2793 3000 3087 3184 3466 3592 3868
Size: 35 30 207 87 97 282 126 276
Cuts at: 3868 4757 6386 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 673 282 276 275 219
207 126 97 87 54 50 43 35
30 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3868 1537
Size: 275 2056 4090
Fragments arranged by size:
4090 2056 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2222 2816 2876 3518 4351 1353
Size: 869 594 60 642 833 3423
Fragments arranged by size:
3423 869 833 642 594 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5008 5382 5528 387
Size: 1064 138 3419 374 146 1280
Fragments arranged by size:
3419 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2857 2857
Size: 6421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2150 2450 2509 2536 3180 3342 3395
Size: 393 518 300 59 27 644 162 53
Cuts at: 3395 4484 5081 5868 6136 1239
Size: 1089 597 787 268 1524
Fragments arranged by size:
1524 1089 787 644 597 518 393 300
268 162 59 53 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2468 2591 3604 2468
Size: 123 1013 5285
Fragments arranged by size:
5285 1013 123
EcoO109I rG'GnC_Cy
Cuts at: 2187 2322 2473 2665 2666 2685 2716 2923 2924
Size: 135 151 192 1 19 31 207 1
Cuts at: 2924 3190 3391 3392 2187
Size: 266 201 1 5216
Fragments arranged by size:
5216 266 207 201 192 151 135 31
19 1 1 1
EcoRI G'AATT_C
Cuts at: 3730 4108 3730
Size: 378 6043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3281 3281
Size: 6421
FspI TGC'GCA
Cuts at: 2267 4927 2267
Size: 2660 3761
FspAI rTGC'GCAy
Cuts at: 2267 2267
Size: 6421
HaeII r_GCGC'y
Cuts at: 1678 2496 3589 5568 1678
Size: 818 1093 1979 2531
Fragments arranged by size:
2531 1979 1093 818
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5062 5094 5136 5168 1373
Size: 32 135 32 3490 32 42 32 2626
Fragments arranged by size:
3490 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3765 4011 669
Size: 988 281 1827 246 3079
Fragments arranged by size:
3079 1827 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6421
HpaI GTT'AAC
Cuts at: 4011 4011
Size: 6421
KpnI G_GTAC'C
Cuts at: 2637 3746 2637
Size: 1109 5312
MfeI C'AATT_G
Cuts at: 4020 4020
Size: 6421
MluI A'CGCG_T
Cuts at: 6297 6297
Size: 6421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5535 5719 1305
Size: 4230 184 2007
Fragments arranged by size:
4230 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2051 2765 2795 3089 3468
Size: 54 1425 343 219 714 30 294 379
Cuts at: 3468 10
Size: 2963
Fragments arranged by size:
2963 1425 714 379 343 294 219 54
30
MslI CAynn'nnrTG
Cuts at: 518 1166 3656 4379 4738 4897 518
Size: 648 2490 723 359 159 2042
Fragments arranged by size:
2490 2042 723 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2281 2566 2709 3006 3356 3532 3558 3837
Size: 675 285 143 297 350 176 26 279
Cuts at: 3837 4514 5662 5907 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 675 350 297 285 279
245 176 143 26
NcoI C'CATG_G
Cuts at: 513 1065 2004 2052 513
Size: 552 939 48 4882
Fragments arranged by size:
4882 939 552 48
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6421
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6421
NotI GC'GGCC_GC
Cuts at: 3868 3868
Size: 6421
NspI r_CATG'y
Cuts at: 1132 1229 2029 3685 3779 5324 1132
Size: 97 800 1656 94 1545 2229
Fragments arranged by size:
2229 1656 1545 800 97 94
PasI CC'CwG_GG
Cuts at: 1348 2412 2914 3487 1348
Size: 1064 502 573 4282
Fragments arranged by size:
4282 1064 573 502
PciI A'CATG_T
Cuts at: 2025 5320 2025
Size: 3295 3126
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2529 1108
Size: 1421 5000
PmeI GTTT'AAAC
Cuts at: 3725 3725
Size: 6421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 6003 6035 4490
Size: 32 1481 32 4876
Fragments arranged by size:
4876 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2187 2187
Size: 6421
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6421
PsiI TTA'TAA
Cuts at: 3991 3991
Size: 6421
PspOMI G'GGCC_C
Cuts at: 2665 2923 3391 2665
Size: 258 468 5695
Fragments arranged by size:
5695 468 258
PstI C_TGCA'G
Cuts at: 830 2373 2897 3773 830
Size: 1543 524 876 3478
Fragments arranged by size:
3478 1543 876 524
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6421
PvuII CAG'CTG
Cuts at: 2709 3532 2709
Size: 823 5598
SacI G_AGCT'C
Cuts at: 2859 2859
Size: 6421
SacII CC_GC'GG
Cuts at: 3357 3357
Size: 6421
SalI G'TCGA_C
Cuts at: 1936 3763 1936
Size: 1827 4594
SbfI CC_TGCA'GG
Cuts at: 2373 3773 2373
Size: 1400 5021
ScaI AGT'ACT
Cuts at: 4669 6381 4669
Size: 1712 4709
SexAI A'CCwGG_T
Cuts at: 2646 2646
Size: 6421
SfcI C'TryA_G
Cuts at: 826 1045 2369 2893 3769 4904 5585 5776 826
Size: 219 1324 524 876 1135 681 191 1471
Fragments arranged by size:
1471 1324 1135 876 681 524 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6421
SmaI CCC'GGG
Cuts at: 1872 2112 2305 2328 3700 3748 1872
Size: 240 193 23 1372 48 4545
Fragments arranged by size:
4545 1372 240 193 48 23
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2158 2858 4525 5426 5688 820
Size: 197 926 215 700 1667 901 262 1553
Fragments arranged by size:
1667 1553 926 901 700 262 215 197
SnaBI TAC'GTA
Cuts at: 493 4212 493
Size: 3719 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6421
SphI G_CATG'C
Cuts at: 1132 3779 1132
Size: 2647 3774
SspI AAT'ATT
Cuts at: 5 52 4345 5
Size: 47 4293 2081
Fragments arranged by size:
4293 2081 47
StuI AGG'CCT
Cuts at: 1517 2420 2441 3101 1517
Size: 903 21 660 4837
Fragments arranged by size:
4837 903 660 21
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 2587 3292 3508 3860 513
Size: 552 939 48 535 705 216 352 3074
Fragments arranged by size:
3074 939 705 552 535 352 216 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3552 3634 4448 4465 4618 4803 5248
Size: 1255 1972 82 814 17 153 185 445
Cuts at: 5248 325
Size: 1498
Fragments arranged by size:
1972 1498 1255 814 445 185 153 82
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4667 6379 96
Size: 274 80 33 51 1443 2690 1712 138
Fragments arranged by size:
2690 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6381 6413 6381
Size: 32 6389
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6421
XbaI T'CTAG_A
Cuts at: 3757 3757
Size: 6421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2484 2484
Size: 6421
XhoI C'TCGA_G
Cuts at: 1943 2158 1943
Size: 215 6206
XmaI C'CCGG_G
Cuts at: 1870 2110 2303 2326 3698 3746 1870
Size: 240 193 23 1372 48 4545
Fragments arranged by size:
4545 1372 240 193 48 23
XmnI GAAnn'nnTTC
Cuts at: 4550 4550
Size: 6421
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2077 4229 276
Size: 53 83 186 936 543 2152 2468
Fragments arranged by size:
2468 2152 936 543 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI