Restriction map for
pFN21AE1299
(Circular) (Six-base) MAPSORT of: pFN21AE1299.seq Check: 5182 from: 1 to: 5470
pFN21AE1299 5470 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3280 278
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2813 1937
Size: 876 4594
Acc65I G'GTAC_C
Cuts at: 2791 2791
Size: 5470
AclI AA'CG_TT
Cuts at: 1563 3597 3970 1563
Size: 2034 373 3063
Fragments arranged by size:
3063 2034 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3533 4917 5185 1632
Size: 1901 1384 268 1917
Fragments arranged by size:
1917 1901 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5273
AflIII A'CryG_T
Cuts at: 1203 4369 5346 1203
Size: 3166 977 1327
Fragments arranged by size:
3166 1327 977
AgeI A'CCGG_T
Cuts at: 4301 4301
Size: 5470
AhdI GACnn_n'nnGTC
Cuts at: 2379 4199 2379
Size: 1820 3650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5470
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5470
AlwNI CAG_nnn'CTG
Cuts at: 2657 3213 4785 2657
Size: 556 1572 3342
Fragments arranged by size:
3342 1572 556
ApaI G_GGCC'C
Cuts at: 2038 2766 2038
Size: 728 4742
ApaLI G'TGCA_C
Cuts at: 2256 3527 4683 2256
Size: 1271 1156 3043
Fragments arranged by size:
3043 1271 1156
ApoI r'AATT_y
Cuts at: 1493 2156 2231 2779 3005 3157 5266 1493
Size: 663 75 548 226 152 2109 1697
Fragments arranged by size:
2109 1697 663 548 226 152 75
AseI AT'TA_AT
Cuts at: 160 1032 4024 160
Size: 872 2992 1606
Fragments arranged by size:
2992 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
AvaI C'yCGr_G
Cuts at: 1870 1943 2795 1870
Size: 73 852 4545
Fragments arranged by size:
4545 852 73
BamHI G'GATC_C
Cuts at: 2800 3167 2800
Size: 367 5103
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2791 4246 618
Size: 325 217 627 1004 1455 1842
Fragments arranged by size:
1842 1455 1004 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2038 2063 2649 2766 2789 1391
Size: 647 25 586 117 23 4072
Fragments arranged by size:
4072 647 586 117 25 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2082 2707 928
Size: 971 183 625 3691
Fragments arranged by size:
3691 971 625 183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3661 3695 1903
Size: 34 1724 34 3678
Fragments arranged by size:
3678 1724 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3362 4572 3362
Size: 1210 4260
BclI T'GATC_A
Cuts at: 1548 2506 1548
Size: 958 4512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2694 4081 136
Size: 107 122 71 631 605 1022 1387 1525
Fragments arranged by size:
1525 1387 1022 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5465 5465
Size: 5470
BlpI GC'TnA_GC
Cuts at: 2447 2887 2447
Size: 440 5030
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2260 2460 2671 2711 2766 3531 4687
Size: 248 222 200 211 40 55 765 1156
Cuts at: 4687 1790
Size: 2573
Fragments arranged by size:
2573 1156 765 248 222 211 200 55
40
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5470
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2387 2593 2623
Size: 416 53 83 399 24 970 206 30
Cuts at: 2623 3201 4159 442
Size: 578 958 1753
Fragments arranged by size:
1753 970 958 578 416 399 206 83
53 30 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5470
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 4130 1239
Size: 971 1920 2579
Fragments arranged by size:
2579 1920 971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3595 4460 4758 3595
Size: 865 298 4307
Fragments arranged by size:
4307 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2477 4133 882
Size: 617 978 1656 2219
Fragments arranged by size:
2219 1656 978 617
BsaAI yAC'GTr
Cuts at: 493 1204 2182 3261 493
Size: 711 978 1079 2702
Fragments arranged by size:
2702 1079 978 711
BsaBI GATnn'nnATC
Cuts at: 5464 5464
Size: 5470
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3277 3659 4294 275
Size: 53 83 186 936 1744 382 635 1451
Fragments arranged by size:
1744 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3903 4301 4575 4722 5133 3903
Size: 398 274 147 411 4240
Fragments arranged by size:
4240 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5245
Fragments arranged by size:
5245 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5470
BseYI C'CCAG_C
Cuts at: 2029 4673 5169 2029
Size: 2644 496 2330
Fragments arranged by size:
2644 2330 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4897
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2920 3681 4709 1540
Size: 275 186 919 761 1028 2301
Fragments arranged by size:
2301 1028 919 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2260 2583 2649 3531 3616 4687 2260
Size: 323 66 882 85 1071 3043
Fragments arranged by size:
3043 1071 882 323 85 66
BsmI GAATG_Cn'
Cuts at: 2981 3074 2981
Size: 93 5377
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2063 2260 2460 2583 2649 2671
Size: 399 248 25 197 200 123 66 22
Cuts at: 2671 2711 2766 2789 3531 3616 4687 1391
Size: 40 55 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 248 200 197 123
85 66 55 40 25 23 22
BspEI T'CCGG_A
Cuts at: 5133 5133
Size: 5470
BspHI T'CATG_A
Cuts at: 2298 3359 5089 2298
Size: 1061 1730 2679
Fragments arranged by size:
2679 1730 1061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2825 844
Size: 1981 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3357 1394
Size: 429 1534 3507
Fragments arranged by size:
3507 1534 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2458 3965 4139 65
Size: 1183 1210 1507 174 1396
Fragments arranged by size:
1507 1396 1210 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4114 4301 1700
Size: 110 2304 187 2869
Fragments arranged by size:
2869 2304 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5470
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5470
BssSI C'ACGA_G
Cuts at: 2690 3530 4542 2690
Size: 840 1012 3618
Fragments arranged by size:
3618 1012 840
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5470
BstBI TT'CG_AA
Cuts at: 4274 4274
Size: 5470
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5470
BstYI r'GATC_y
Cuts at: 1972 2800 3167 3552 3569 5021 5107 5119 5465
Size: 828 367 385 17 1452 86 12 346
Cuts at: 5465 1972
Size: 1977
Fragments arranged by size:
1977 1452 828 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2343 2343
Size: 5470
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4918
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4731
Fragments arranged by size:
4731 683 56
BtsI GCAGTG_nn'
Cuts at: 2986 3780 3800 2986
Size: 794 20 4656
Fragments arranged by size:
4656 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5435
DraI TTT'AAA
Cuts at: 2774 2774
Size: 5470
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4477 809
Size: 507 3161 1802
Fragments arranged by size:
3161 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2140 2917 3806
Size: 54 1425 50 275 18 310 777 889
Cuts at: 3806 5435 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 777 310 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2917 1537
Size: 275 1105 4090
Fragments arranged by size:
4090 1105 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2607 3400 1353
Size: 1254 793 3423
Fragments arranged by size:
3423 1254 793
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4057 4431 4577 387
Size: 1064 138 2468 374 146 1280
Fragments arranged by size:
2468 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2647 2647
Size: 5470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 3533 4130 4917 5185 1239
Size: 393 578 1323 597 787 268 1524
Fragments arranged by size:
1524 1323 787 597 578 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2034 2035 2587 2762 2763 2034
Size: 1 552 175 1 4741
Fragments arranged by size:
4741 552 175 1 1
EcoRI G'AATT_C
Cuts at: 2779 3157 2779
Size: 378 5092
FspI TGC'GCA
Cuts at: 3976 3976
Size: 5470
HaeII r_GCGC'y
Cuts at: 1678 4617 1678
Size: 2939 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2365 2397 4111 4143 4185
Size: 32 135 32 793 32 1714 32 42
Cuts at: 4185 4217 1373
Size: 32 2626
Fragments arranged by size:
2626 1714 793 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2434 2814 3060 669
Size: 988 281 496 380 246 3079
Fragments arranged by size:
3079 988 496 380 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5470
HpaI GTT'AAC
Cuts at: 3060 3060
Size: 5470
KpnI G_GTAC'C
Cuts at: 2795 2795
Size: 5470
MfeI C'AATT_G
Cuts at: 3069 3069
Size: 5470
MluI A'CGCG_T
Cuts at: 5346 5346
Size: 5470
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4584 4768 1305
Size: 3279 184 2007
Fragments arranged by size:
3279 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2142 10
Size: 54 1425 343 310 3338
Fragments arranged by size:
3338 1425 343 310 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 2273 2702 3428 3787 3946 518
Size: 648 888 219 429 726 359 159 2042
Fragments arranged by size:
2042 888 726 648 429 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 2324 2886 3563 4711 4956 1606
Size: 718 562 677 1148 245 2120
Fragments arranged by size:
2120 1148 718 677 562 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4918
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5470
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5470
NotI GC'GGCC_GC
Cuts at: 2917 2917
Size: 5470
NspI r_CATG'y
Cuts at: 1132 1229 2828 4373 1132
Size: 97 1599 1545 2229
Fragments arranged by size:
2229 1599 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2671 1348
Size: 1323 4147
PciI A'CATG_T
Cuts at: 4369 4369
Size: 5470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5470
PmeI GTTT'AAAC
Cuts at: 2774 2774
Size: 5470
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3539 3571 5052 5084 3539
Size: 32 1481 32 3925
Fragments arranged by size:
3925 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2660 1613
Size: 1047 4423
PsiI TTA'TAA
Cuts at: 3040 3040
Size: 5470
PspOMI G'GGCC_C
Cuts at: 2034 2762 2034
Size: 728 4742
PstI C_TGCA'G
Cuts at: 830 2822 830
Size: 1992 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5470
SacI G_AGCT'C
Cuts at: 2649 2649
Size: 5470
SalI G'TCGA_C
Cuts at: 1936 2812 1936
Size: 876 4594
SbfI CC_TGCA'GG
Cuts at: 2822 2822
Size: 5470
ScaI AGT'ACT
Cuts at: 3718 5430 3718
Size: 1712 3758
SfcI C'TryA_G
Cuts at: 826 1045 2818 3953 4634 4825 826
Size: 219 1773 1135 681 191 1471
Fragments arranged by size:
1773 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5470
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5470
SmaI CCC'GGG
Cuts at: 1872 2797 1872
Size: 925 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3574 4475 4737 820
Size: 197 926 1631 901 262 1553
Fragments arranged by size:
1631 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3261 493
Size: 2768 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5470
SphI G_CATG'C
Cuts at: 1132 2828 1132
Size: 1696 3774
SspI AAT'ATT
Cuts at: 5 52 3394 5
Size: 47 3342 2081
Fragments arranged by size:
3342 2081 47
StuI AGG'CCT
Cuts at: 1517 2730 1517
Size: 1213 4257
StyI C'CwwG_G
Cuts at: 513 1065 2725 2758 2766 2909 513
Size: 552 1660 33 8 143 3074
Fragments arranged by size:
3074 1660 552 143 33 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2165 3497 3514 3667 3852 4297 325
Size: 1255 585 1332 17 153 185 445 1498
Fragments arranged by size:
1498 1332 1255 585 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3716 5428 96
Size: 274 80 33 51 1443 1739 1712 138
Fragments arranged by size:
1739 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5430 5462 5430
Size: 32 5438
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5470
XbaI T'CTAG_A
Cuts at: 2806 2806
Size: 5470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2159 2159
Size: 5470
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5470
XmaI C'CCGG_G
Cuts at: 1870 2795 1870
Size: 925 4545
XmnI GAAnn'nnTTC
Cuts at: 3599 3599
Size: 5470
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3278 276
Size: 53 83 186 936 1744 2468
Fragments arranged by size:
2468 1744 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI