Restriction map for
pFN21AE1296
(Circular) (Six-base) MAPSORT of: pFN21AE1296.seq Check: 4759 from: 1 to: 6232
pFN21AE1296 6232 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3495 3495
Size: 6232
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3575 1937
Size: 1638 4594
Acc65I G'GTAC_C
Cuts at: 2722 3553 2722
Size: 831 5401
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2121 2331 4295 5679 5947 1632
Size: 489 210 1964 1384 268 1917
Fragments arranged by size:
1964 1917 1384 489 268 210
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 2750 5131 6108 1203
Size: 1547 2381 977 1327
Fragments arranged by size:
2381 1547 1327 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 2909 1166
Size: 1743 4489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 6200
AlwNI CAG_nnn'CTG
Cuts at: 2366 2955 3262 3499 3975 5547 2366
Size: 589 307 237 476 1572 3051
Fragments arranged by size:
3051 1572 589 476 307 237
ApaI G_GGCC'C
Cuts at: 3169 3169
Size: 6232
ApaLI G'TGCA_C
Cuts at: 2693 4289 5445 2693
Size: 1596 1156 3480
Fragments arranged by size:
3480 1596 1156
ApoI r'AATT_y
Cuts at: 1493 2080 2474 2534 3541 3767 3919 6028 1493
Size: 587 394 60 1007 226 152 2109 1697
Fragments arranged by size:
2109 1697 1007 587 394 226 152 60
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 2272 2375 3125 3557 1870
Size: 73 329 103 750 432 4545
Fragments arranged by size:
4545 750 432 329 103 73
BamHI G'GATC_C
Cuts at: 2859 3562 3929 2859
Size: 703 367 5162
Fragments arranged by size:
5162 703 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2260 2722 3553 5008 618
Size: 325 217 627 473 462 831 1455 1842
Fragments arranged by size:
1842 1455 831 627 473 462 325 217
BanII G_rGCy'C
Cuts at: 1391 2273 2901 3169 3201 3322 3551 1391
Size: 882 628 268 32 121 229 4072
Fragments arranged by size:
4072 882 628 268 229 121 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3059 928
Size: 971 1160 4101
Fragments arranged by size:
4101 1160 971
BbvCI CC'TCA_GC
Cuts at: 2286 2286
Size: 6232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4124 5334 4124
Size: 1210 5022
BclI T'GATC_A
Cuts at: 1548 2160 3402 1548
Size: 612 1242 4378
Fragments arranged by size:
4378 1242 612
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4843 136
Size: 107 122 71 631 605 3171 1525
Fragments arranged by size:
3171 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6227 6227
Size: 6232
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 2697 2758 3169 4293 5449 1790
Size: 907 61 411 1124 1156 2573
Fragments arranged by size:
2573 1156 1124 907 411 61
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6232
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3963 4921 442
Size: 416 53 83 399 24 2546 958 1753
Fragments arranged by size:
2546 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2098 2251 3065 3215 3521 4892 1239
Size: 859 153 814 150 306 1371 2579
Fragments arranged by size:
2579 1371 859 814 306 153 150
Bpu10I CC'TnA_GC
Cuts at: 2286 2286
Size: 6232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2702 3233 4357 5222 5520 2702
Size: 531 1124 865 298 3414
Fragments arranged by size:
3414 1124 865 531 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4895 882
Size: 617 3396 2219
Fragments arranged by size:
3396 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4023 493
Size: 711 2819 2702
Fragments arranged by size:
2819 2702 711
BsaBI GATnn'nnATC
Cuts at: 6226 6226
Size: 6232
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4039 4421 5056 275
Size: 53 83 186 936 2506 382 635 1451
Fragments arranged by size:
2506 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2200 2397 4665 5063 5337 5484 5895 2200
Size: 197 2268 398 274 147 411 2537
Fragments arranged by size:
2537 2268 411 398 274 197 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3266 3296 1178
Size: 30 165 30 1863 30 4114
Fragments arranged by size:
4114 1863 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3143 3242 1186
Size: 1957 99 4176
Fragments arranged by size:
4176 1957 99
BseYI C'CCAG_C
Cuts at: 2024 2091 2924 5435 5931 2024
Size: 67 833 2511 496 2325
Fragments arranged by size:
2511 2325 833 496 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2968 3135 1163
Size: 573 1232 167 4260
Fragments arranged by size:
4260 1232 573 167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3682 4443 5471 1540
Size: 275 186 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2697 2854 2990 4293 4378 5449 2697
Size: 157 136 1303 85 1071 3480
Fragments arranged by size:
3480 1303 1071 157 136 85
BsmI GAATG_Cn'
Cuts at: 2496 3743 3836 2496
Size: 1247 93 4892
Fragments arranged by size:
4892 1247 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2273 2697 2758 2854 2901 2990 3169
Size: 399 483 424 61 96 47 89 179
Cuts at: 3169 3201 3322 3551 4293 4378 5449 1391
Size: 32 121 229 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 483 424 399 229 179
121 96 89 85 61 47 32
BspEI T'CCGG_A
Cuts at: 2200 2397 5895 2200
Size: 197 3498 2537
Fragments arranged by size:
3498 2537 197
BspHI T'CATG_A
Cuts at: 4121 5851 4121
Size: 1730 4502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3309 3495 3587 844
Size: 2465 186 92 3489
Fragments arranged by size:
3489 2465 186 92
BsrBI CCG'CTC
Cuts at: 1394 1823 4119 1394
Size: 429 2296 3507
Fragments arranged by size:
3507 2296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2662 2965 4727 4901 65
Size: 1183 1414 303 1762 174 1396
Fragments arranged by size:
1762 1414 1396 1183 303 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2931 4876 5063 1700
Size: 110 1121 1945 187 2869
Fragments arranged by size:
2869 1945 1121 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2955 3262 794
Size: 2161 307 3764
Fragments arranged by size:
3764 2161 307
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6232
BstYI r'GATC_y
Cuts at: 1972 2224 2859 3562 3929 4314 4331 5783 5869
Size: 252 635 703 367 385 17 1452 86
Cuts at: 5869 5881 6227 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 703 635 385 367 346 252
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2865 3333 2865
Size: 468 5764
BtgI C'CryG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 4231
Fragments arranged by size:
4231 1449 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2560 487
Size: 683 56 1334 4159
Fragments arranged by size:
4159 1334 683 56
BtsI GCAGTG_nn'
Cuts at: 2038 2163 3748 4542 4562 2038
Size: 125 1585 794 20 3708
Fragments arranged by size:
3708 1585 794 125 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 3536 3536
Size: 6232
DraIII CAC_nnn'GTG
Cuts at: 3241 3241
Size: 6232
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5239 809
Size: 507 3923 1802
Fragments arranged by size:
3923 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 3679 4568
Size: 54 1425 50 275 18 465 1384 889
Cuts at: 4568 6197 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1384 889 465 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2307 3368 3417 4162 1353
Size: 954 1061 49 745 3423
Fragments arranged by size:
3423 1061 954 745 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4819 5193 5339 387
Size: 1064 138 3230 374 146 1280
Fragments arranged by size:
3230 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2121 2251 2331 3065 3215 3521
Size: 393 466 23 130 80 734 150 306
Cuts at: 3521 4295 4892 5679 5947 1239
Size: 774 597 787 268 1524
Fragments arranged by size:
1524 787 774 734 597 466 393 306
268 150 130 80 23
EcoNI CCTnn'n_nnAGG
Cuts at: 3491 3491
Size: 6232
EcoO109I rG'GnC_Cy
Cuts at: 2524 2973 3040 3165 3166 3496 2524
Size: 449 67 125 1 330 5260
Fragments arranged by size:
5260 449 330 125 67 1
EcoRI G'AATT_C
Cuts at: 2080 3541 3919 2080
Size: 1461 378 4393
Fragments arranged by size:
4393 1461 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3076 3403 3076
Size: 327 5905
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 2942 5379 1678
Size: 1264 2437 2531
Fragments arranged by size:
2531 2437 1264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2269 2301 4873 4905 4947
Size: 32 135 32 697 32 2572 32 42
Cuts at: 4947 4979 1373
Size: 32 2626
Fragments arranged by size:
2626 2572 697 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2064 3298 3385 3576 3822 669
Size: 988 281 126 1234 87 191 246 3079
Fragments arranged by size:
3079 1234 988 281 246 191 126 87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KpnI G_GTAC'C
Cuts at: 2726 3557 2726
Size: 831 5401
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3252 5346 5530 1305
Size: 1947 2094 184 2007
Fragments arranged by size:
2094 2007 1947 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 10
Size: 54 1425 343 465 3945
Fragments arranged by size:
3945 1425 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2690 2909 4190 4549 4708 518
Size: 648 1002 522 219 1281 359 159 2042
Fragments arranged by size:
2042 1281 1002 648 522 359 219 159
MspA1I CmG'CkG
Cuts at: 1606 2194 2736 3193 3475 3648 4325 5473 5718
Size: 588 542 457 282 173 677 1148 245
Cuts at: 5718 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 588 542 457 282 245
173
NcoI C'CATG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 4231
Fragments arranged by size:
4231 1449 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6232
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 2754 3590 5135 1132
Size: 97 1525 836 1545 2229
Fragments arranged by size:
2229 1545 1525 836 97
PasI CC'CwG_GG
Cuts at: 1348 2758 1348
Size: 1410 4822
PciI A'CATG_T
Cuts at: 2750 5131 2750
Size: 2381 3851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2683 3266 3298 4301 4333 5814 5846 2651
Size: 32 583 32 1003 32 1481 32 3037
Fragments arranged by size:
3037 1481 1003 583 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2524 3496 2524
Size: 972 5260
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PspOMI G'GGCC_C
Cuts at: 3165 3165
Size: 6232
PstI C_TGCA'G
Cuts at: 830 3267 3474 3584 830
Size: 2437 207 110 3478
Fragments arranged by size:
3478 2437 207 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 2194 2736 3475 2194
Size: 542 739 4951
Fragments arranged by size:
4951 739 542
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 2210 3362 4480 6192 2210
Size: 1152 1118 1712 2250
Fragments arranged by size:
2250 1712 1152 1118
SfcI C'TryA_G
Cuts at: 826 1045 3263 3470 3580 4715 5396 5587 826
Size: 219 2218 207 110 1135 681 191 1471
Fragments arranged by size:
2218 1471 1135 681 219 207 191 110
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 2274 3559 1872
Size: 402 1285 4545
Fragments arranged by size:
4545 1285 402
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2717 3212 4336 5237 5499 820
Size: 197 926 774 495 1124 901 262 1553
Fragments arranged by size:
1553 1124 926 901 774 495 262 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3590 1132
Size: 2458 3774
SspI AAT'ATT
Cuts at: 5 52 4156 5
Size: 47 4104 2081
Fragments arranged by size:
4104 2081 47
StuI AGG'CCT
Cuts at: 1517 2589 2702 1517
Size: 1072 113 5047
Fragments arranged by size:
5047 1072 113
StyI C'CwwG_G
Cuts at: 513 1065 2514 2907 3671 513
Size: 552 1449 393 764 3074
Fragments arranged by size:
3074 1449 764 552 393
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4259 4276 4429 4614 5059 325
Size: 1255 2679 17 153 185 445 1498
Fragments arranged by size:
2679 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 2346 3360
Size: 274 80 33 51 1443 231 138 1014
Cuts at: 3360 4478 6190 96
Size: 1118 1712 138
Fragments arranged by size:
1712 1443 1118 1014 274 231 138 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6192 6224 6192
Size: 32 6200
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2639 2705 2733 2639
Size: 66 28 6138
Fragments arranged by size:
6138 66 28
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 2272 3557 1870
Size: 402 1285 4545
Fragments arranged by size:
4545 1285 402
XmnI GAAnn'nnTTC
Cuts at: 2134 4361 2134
Size: 2227 4005
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI