Restriction map for
pFN21AE1295
(Circular) (Six-base) MAPSORT of: pFN21AE1295.seq Check: 2334 from: 1 to: 6217
pFN21AE1295 6217 bp
With 182 enzymes: SgfI *
January 31, 2011 17:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2601 2685 4280 5664 5932 1632
Size: 969 84 1595 1384 268 1917
Fragments arranged by size:
1917 1595 1384 969 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 2522 5116 6093 1203
Size: 1319 2594 977 1327
Fragments arranged by size:
2594 1327 1319 977
AgeI A'CCGG_T
Cuts at: 3381 5048 3381
Size: 1667 4550
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 3960 5532 3960
Size: 1572 4645
ApaLI G'TGCA_C
Cuts at: 2282 4274 5430 2282
Size: 1992 1156 3069
Fragments arranged by size:
3069 1992 1156
ApoI r'AATT_y
Cuts at: 1493 2236 2375 2383 2503 2655 2907 3075 3327
Size: 743 139 8 120 152 252 168 252
Cuts at: 3327 3411 3526 3752 3904 6013 1493
Size: 84 115 226 152 2109 1697
Fragments arranged by size:
2109 1697 743 252 252 226 168 152
152 139 120 115 84 8
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 3542 1870
Size: 73 1599 4545
Fragments arranged by size:
4545 1599 73
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3538 4993 618
Size: 325 217 627 1751 1455 1842
Fragments arranged by size:
1842 1751 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2094 3536 1391
Size: 703 1442 4072
Fragments arranged by size:
4072 1442 703
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 928
Size: 971 124 5122
Fragments arranged by size:
5122 971 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 2064 1548
Size: 516 5701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3212 4828 136
Size: 107 122 71 631 605 1540 1616 1525
Fragments arranged by size:
1616 1540 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 3634 3634
Size: 6217
Bme1580I G_kGCm'C
Cuts at: 1790 2286 4278 5434 1790
Size: 496 1992 1156 2573
Fragments arranged by size:
2573 1992 1156 496
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2126 3948 4906
Size: 416 53 83 399 24 709 1822 958
Cuts at: 4906 442
Size: 1753
Fragments arranged by size:
1822 1753 958 709 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2853 2937 3021 3189 3273 3357 3441
Size: 857 757 84 84 168 84 84 84
Cuts at: 3441 4877 1239
Size: 1436 2579
Fragments arranged by size:
2579 1436 857 757 168 84 84 84
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4342 5207 5505 4342
Size: 865 298 5054
Fragments arranged by size:
5054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4880 882
Size: 617 3381 2219
Fragments arranged by size:
3381 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4024 4406 5041 275
Size: 53 83 186 936 2491 382 635 1451
Fragments arranged by size:
2491 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3381 4650 5048 5322 5469 5880 3381
Size: 1269 398 274 147 411 3718
Fragments arranged by size:
3718 1269 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5992
Fragments arranged by size:
5992 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6217
BseYI C'CCAG_C
Cuts at: 5420 5916 5420
Size: 496 5721
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5644
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2286 4278 4363 5434 2286
Size: 1992 85 1071 3069
Fragments arranged by size:
3069 1992 1071 85
BsmI GAATG_Cn'
Cuts at: 3215 3728 3821 3215
Size: 513 93 5611
Fragments arranged by size:
5611 513 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2286 3536 4278 4363 5434 1391
Size: 399 304 192 1250 742 85 1071 2174
Fragments arranged by size:
2174 1250 1071 742 399 304 192 85
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3572 844
Size: 2728 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4104 1394
Size: 429 2281 3507
Fragments arranged by size:
3507 2281 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2860 3280 4712 4886 65
Size: 1183 1612 420 1432 174 1396
Fragments arranged by size:
1612 1432 1396 1183 420 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3381 4861 5048 1700
Size: 110 1571 1480 187 2869
Fragments arranged by size:
2869 1571 1480 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2281 794
Size: 1487 4730
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstYI r'GATC_y
Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212
Size: 1575 367 385 17 1452 86 12 346
Cuts at: 6212 1972
Size: 1977
Fragments arranged by size:
1977 1575 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5665
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2129 487
Size: 683 56 903 4575
Fragments arranged by size:
4575 903 683 56
BtsI GCAGTG_nn'
Cuts at: 3208 3733 4527 4547 3208
Size: 525 794 20 4878
Fragments arranged by size:
4878 794 525 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2842 2877 3262 3297 529
Size: 35 2278 35 385 35 3449
Fragments arranged by size:
3449 2278 385 35 35 35
DraI TTT'AAA
Cuts at: 3043 3521 3043
Size: 478 5739
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2165 3664 4553
Size: 54 1425 50 275 18 335 1499 889
Cuts at: 4553 6182 8
Size: 1629 43
Fragments arranged by size:
1629 1499 1425 889 335 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2661 4147 1353
Size: 746 562 1486 3423
Fragments arranged by size:
3423 1486 746 562
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4804 5178 5324 387
Size: 1064 138 3215 374 146 1280
Fragments arranged by size:
3215 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2601 2685 2853 2937 3021 3189
Size: 393 464 505 84 168 84 84 168
Cuts at: 3189 3273 3357 3441 4280 4877 5664 5932 1239
Size: 84 84 84 839 597 787 268 1524
Fragments arranged by size:
1524 839 787 597 505 464 393 268
168 168 84 84 84 84 84 84
EcoRI G'AATT_C
Cuts at: 2907 3327 3526 3904 2907
Size: 420 199 378 5220
Fragments arranged by size:
5220 420 378 199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2194
Size: 6217
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 5364 1678
Size: 3686 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2056 2088 2560 2592 4858
Size: 32 135 32 484 32 472 32 2266
Cuts at: 4858 4890 4932 4964 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2266 484 472 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3561 3807 669
Size: 988 281 1623 246 3079
Fragments arranged by size:
3079 1623 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2952 3372 748
Size: 2204 420 3593
Fragments arranged by size:
3593 2204 420
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 2764 3816 2764
Size: 1052 5165
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5331 5515 1305
Size: 4026 184 2007
Fragments arranged by size:
4026 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2167 10
Size: 54 1425 343 335 4060
Fragments arranged by size:
4060 1425 343 335 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3100 3504 4175 4534 4693 518
Size: 648 1934 404 671 359 159 2042
Fragments arranged by size:
2042 1934 671 648 404 359 159
MspA1I CmG'CkG
Cuts at: 1606 3633 4310 5458 5703 1606
Size: 2027 677 1148 245 2120
Fragments arranged by size:
2120 2027 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5665
NdeI CA'TA_TG
Cuts at: 387 3501 387
Size: 3114 3103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 2114 2526 3575 5120 1132
Size: 97 885 412 1049 1545 2229
Fragments arranged by size:
2229 1545 1049 885 412 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 2522 5116 2522
Size: 2594 3623
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 2298 3787 2298
Size: 1489 4728
PstI C_TGCA'G
Cuts at: 830 3569 830
Size: 2739 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SexAI A'CCwGG_T
Cuts at: 2025 2025
Size: 6217
SfcI C'TryA_G
Cuts at: 826 1045 2017 3565 4700 5381 5572 826
Size: 219 972 1548 1135 681 191 1471
Fragments arranged by size:
1548 1471 1135 972 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4321 5222 5484 820
Size: 197 926 2378 901 262 1553
Fragments arranged by size:
2378 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 2340 4141 5
Size: 47 2288 1801 2081
Fragments arranged by size:
2288 2081 1801 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6217
StyI C'CwwG_G
Cuts at: 513 1065 3656 513
Size: 552 2591 3074
Fragments arranged by size:
3074 2591 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3494 4244 4261 4414 4599 5044 325
Size: 1255 1914 750 17 153 185 445 1498
Fragments arranged by size:
1914 1498 1255 750 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4463 6175 96
Size: 274 80 33 51 1443 2486 1712 138
Fragments arranged by size:
2486 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6177 6209 6177
Size: 32 6185
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3482 3553 3482
Size: 71 6146
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 2423 4346 2423
Size: 1923 4294
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI