Restriction map for
pFN21AE1294
(Circular) (Six-base) MAPSORT of: pFN21AE1294.seq Check: 7373 from: 1 to: 6130
pFN21AE1294 6130 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3473 1937
Size: 1536 4594
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 3396 4257 4630 1563
Size: 1833 861 373 3063
Fragments arranged by size:
3063 1833 861 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2417 2441 3025 3054 3346 4193 5577 5845
Size: 785 24 584 29 292 847 1384 268
Cuts at: 5845 1632
Size: 1917
Fragments arranged by size:
1917 1384 847 785 584 292 268 29
24
AfeI AGC'GCT
Cuts at: 2225 2472 2654 3002 2225
Size: 247 182 348 5353
Fragments arranged by size:
5353 348 247 182
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 3306 5029 6006 1203
Size: 2103 1723 977 1327
Fragments arranged by size:
2103 1723 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 2951 1166
Size: 1785 4345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3191 1184
Size: 2007 4123
AlwNI CAG_nnn'CTG
Cuts at: 2668 2698 2926 2983 3201 3406 3873 5445 2668
Size: 30 228 57 218 205 467 1572 3353
Fragments arranged by size:
3353 1572 467 228 218 205 57 30
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 3439 3665 3817 5926 1493
Size: 1946 226 152 2109 1697
Fragments arranged by size:
2109 1946 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2156 2905 3455 1870
Size: 73 213 749 550 4545
Fragments arranged by size:
4545 749 550 213 73
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 2191 3451 4906 618
Size: 325 217 627 354 50 1260 1455 1842
Fragments arranged by size:
1842 1455 1260 627 354 325 217 50
BanII G_rGCy'C
Cuts at: 1391 2184 2692 3213 3449 1391
Size: 793 508 521 236 4072
Fragments arranged by size:
4072 793 521 508 236
BbeI G_GCGC'C
Cuts at: 2145 2195 2145
Size: 50 6080
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2502 2811 3089 3250 928
Size: 971 603 309 278 161 3808
Fragments arranged by size:
3808 971 603 309 278 161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2336 2370 4321 4355 1903
Size: 34 399 34 1951 34 3678
Fragments arranged by size:
3678 1951 399 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3129 4741 136
Size: 107 122 71 631 605 1457 1612 1525
Fragments arranged by size:
1612 1525 1457 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 2912 4191 5347 1790
Size: 1122 1279 1156 2573
Fragments arranged by size:
2573 1279 1156 1122
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2128 2873 3070
Size: 416 53 83 399 24 711 745 197
Cuts at: 3070 3082 3861 4819 442
Size: 12 779 958 1753
Fragments arranged by size:
1753 958 779 745 711 416 399 197
83 53 24 12
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2624 4790 1239
Size: 1385 2166 2579
Fragments arranged by size:
2579 2166 1385
Bpu10I CC'TnA_GC
Cuts at: 2048 2048
Size: 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2003 2186 2468 2753 4255 5120 5418 2003
Size: 183 282 285 1502 865 298 2715
Fragments arranged by size:
2715 1502 865 298 285 282 183
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2495 2780 4793 882
Size: 617 996 285 2013 2219
Fragments arranged by size:
2219 2013 996 617 285
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 6124 6124
Size: 6130
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2142 2192 2247 2394
Size: 53 83 186 936 609 50 55 147
Cuts at: 2394 3051 3937 4319 4954 275
Size: 657 886 382 635 1451
Fragments arranged by size:
1451 936 886 657 635 609 382 186
147 83 55 53 50
BsaWI w'CCGG_w
Cuts at: 4563 4961 5235 5382 5793 4563
Size: 398 274 147 411 4900
Fragments arranged by size:
4900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2492 2522 1178
Size: 30 165 30 1089 30 4786
Fragments arranged by size:
4786 1089 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2612 1186
Size: 1426 4704
BseYI C'CCAG_C
Cuts at: 2195 2741 3025 3149 5333 5829 2195
Size: 546 284 124 2184 496 2496
Fragments arranged by size:
2496 2184 546 496 284 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2032 2340 2345 2543 2929 3187 1163
Size: 573 296 308 5 198 386 258 4106
Fragments arranged by size:
4106 573 386 308 296 258 198 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2725 3580 4341 5369 1540
Size: 275 186 724 855 761 1028 2301
Fragments arranged by size:
2301 1028 855 761 724 275 186
BsiHKAI G_wGCw'C
Cuts at: 2039 2118 4191 4276 5347 2039
Size: 79 2073 85 1071 2822
Fragments arranged by size:
2822 2073 1071 85 79
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2118 2184 2692 2912 3213 3449
Size: 399 249 79 66 508 220 301 236
Cuts at: 3449 4191 4276 5347 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 508 399 301 249 236
220 85 79 66
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3485 844
Size: 2641 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2455 2554 2707 2764 4017 1394
Size: 429 632 99 153 57 1253 3507
Fragments arranged by size:
3507 1253 632 429 153 99 57
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2163 2381 2562 2724 4774 4961 1700
Size: 110 353 218 181 162 2050 187 2869
Fragments arranged by size:
2869 2050 353 218 187 181 162 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6130
BssHII G'CGCG_C
Cuts at: 1925 2261 2325 1925
Size: 336 64 5730
Fragments arranged by size:
5730 336 64
BssSI C'ACGA_G
Cuts at: 2363 4190 5202 2363
Size: 1827 1012 3291
Fragments arranged by size:
3291 1827 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2122 2566 3351 794
Size: 1328 444 785 3573
Fragments arranged by size:
3573 1328 785 444
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 2377 1184
Size: 1193 4937
BstXI CCAn_nnnn'nTGG
Cuts at: 2218 3386 2218
Size: 1168 4962
BstYI r'GATC_y
Cuts at: 1972 2694 3141 3460 3827 4212 4229 5681 5767
Size: 722 447 319 367 385 17 1452 86
Cuts at: 5767 5779 6125 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 722 447 385 367 346 319
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3154 513
Size: 552 939 1150 3489
Fragments arranged by size:
3489 1150 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2357 2981 3646 4440 4460 2357
Size: 624 665 794 20 4027
Fragments arranged by size:
4027 794 665 624 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DraIII CAC_nnn'GTG
Cuts at: 2122 2881 2122
Size: 759 5371
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2208 3370 3577
Size: 54 1425 50 275 18 378 1162 207
Cuts at: 3577 4466 6095 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1162 889 378 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2312 4060 1353
Size: 959 1748 3423
Fragments arranged by size:
3423 1748 959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2513 2624 3092 4717 5091 5237
Size: 1064 138 924 111 468 1625 374 146
Cuts at: 5237 387
Size: 1280
Fragments arranged by size:
1625 1280 1064 924 468 374 146 138
111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2417 2441 2624 3025 3054 3346 4193
Size: 393 785 24 183 401 29 292 847
Cuts at: 4193 4790 5577 5845 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 847 787 785 597 401 393 292
268 183 29 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2864 2864
Size: 6130
EcoO109I rG'GnC_Cy
Cuts at: 2062 2437 2831 2062
Size: 375 394 5361
Fragments arranged by size:
5361 394 375
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
FspI TGC'GCA
Cuts at: 2672 4636 2672
Size: 1964 4166
HaeII r_GCGC'y
Cuts at: 1678 2145 2195 2227 2474 2656 2926 3004 3201
Size: 467 50 32 247 182 270 78 197
Cuts at: 3201 5277 1678
Size: 2076 2531
Fragments arranged by size:
2531 2076 467 270 247 197 182 78
50 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2492 2524 2679 2711 3293
Size: 32 135 32 920 32 155 32 582
Cuts at: 3293 3325 4771 4803 4845 4877 1373
Size: 32 1446 32 42 32 2626
Fragments arranged by size:
2626 1446 920 582 155 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3474 3720 669
Size: 988 281 1536 246 3079
Fragments arranged by size:
3079 1536 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3239 748
Size: 2491 3639
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KasI G'GCGC_C
Cuts at: 2141 2191 2141
Size: 50 6080
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2916 5244 5428 1305
Size: 1611 2328 184 2007
Fragments arranged by size:
2328 2007 1611 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 3372 10
Size: 54 1425 343 378 1162 2768
Fragments arranged by size:
2768 1425 1162 378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2951 3173 3384 4088 4447 4606 518
Size: 648 1785 222 211 704 359 159 2042
Fragments arranged by size:
2042 1785 704 648 359 222 211 159
MspA1I CmG'CkG
Cuts at: 1606 2103 2242 2479 2668 3156 3406 3546 4223
Size: 497 139 237 189 488 250 140 677
Cuts at: 4223 5371 5616 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 497 488 250 245 237
189 140 139
NaeI GCC'GGC
Cuts at: 2165 2165
Size: 6130
NarI GG'CG_CC
Cuts at: 2142 2192 2142
Size: 50 6080
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
NdeI CA'TA_TG
Cuts at: 387 2800 387
Size: 2413 3717
NgoMIV G'CCGG_C
Cuts at: 2163 2163
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 3310 3488 5033 1132
Size: 97 2081 178 1545 2229
Fragments arranged by size:
2229 2081 1545 178 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 3306 5029 3306
Size: 1723 4407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6130
PfoI T'CCnGG_A
Cuts at: 2679 2865 3231 3261 2679
Size: 186 366 30 5548
Fragments arranged by size:
5548 366 186 30
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 2062
Size: 6130
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2412 2478 2703 2841 2988 3482 830
Size: 1582 66 225 138 147 494 3478
Fragments arranged by size:
3478 1582 494 225 147 138 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 2479 2668 2479
Size: 189 5941
RsrII CG'GwC_CG
Cuts at: 2320 2320
Size: 6130
SacII CC_GC'GG
Cuts at: 3157 3157
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SapI GCTCTTCn'nnn_
Cuts at: 2312 2312
Size: 6130
SbfI CC_TGCA'GG
Cuts at: 2703 3482 2703
Size: 779 5351
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2227 2408 2474 2656 2699 2837 2984
Size: 219 1182 181 66 182 43 138 147
Cuts at: 2984 3478 4613 5294 5485 826
Size: 494 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 681 494 219 191 182
181 147 138 66 43
SfoI GGC'GCC
Cuts at: 2143 2193 2143
Size: 50 6080
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 2907 3457 1872
Size: 1035 550 4545
Fragments arranged by size:
4545 1035 550
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2018 2201 2483 2768 4234 5135
Size: 197 926 75 183 282 285 1466 901
Cuts at: 5135 5397 820
Size: 262 1553
Fragments arranged by size:
1553 1466 926 901 285 282 262 197
183 75
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 2546 1517
Size: 1029 5101
StyI C'CwwG_G
Cuts at: 513 1065 2004 3274 3569 513
Size: 552 939 1270 295 3074
Fragments arranged by size:
3074 1270 939 552 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2110 2645 2892 2988 3082 3242 4157
Size: 1255 530 535 247 96 94 160 915
Cuts at: 4157 4174 4327 4512 4957 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 915 535 530 445 247 185
160 153 96 94 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2823 2841 3192
Size: 274 80 33 51 1443 846 18 351
Cuts at: 3192 4376 6088 96
Size: 1184 1712 138
Fragments arranged by size:
1712 1443 1184 846 351 274 138 80
51 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6090 6122 6090
Size: 32 6098
Tth111I GACn'n_nGTC
Cuts at: 1316 2297 2506 1316
Size: 981 209 4940
Fragments arranged by size:
4940 981 209
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2203
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 2905 3455 1870
Size: 1035 550 4545
Fragments arranged by size:
4545 1035 550
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI
PsrI SacI SanDI SexAI SfiI SrfI SwaI