Restriction map for
pFN21AE1289
(Circular) (Six-base) MAPSORT of: pFN21AE1289.seq Check: 2451 from: 1 to: 7492
pFN21AE1289 7492 bp
With 182 enzymes: SgfI *
March 28, 2012 12:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2419 2419
Size: 7492
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5302 278
Size: 53 83 186 936 3766 2468
Fragments arranged by size:
3766 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2233 4835 1937
Size: 296 2602 4594
Fragments arranged by size:
4594 2602 296
Acc65I G'GTAC_C
Cuts at: 4671 4813 4671
Size: 142 7350
AclI AA'CG_TT
Cuts at: 1563 5619 5992 1563
Size: 4056 373 3063
Fragments arranged by size:
4056 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2309 2577 2583 3021 3270 5555 6939 7207
Size: 677 268 6 438 249 2285 1384 268
Cuts at: 7207 1632
Size: 1917
Fragments arranged by size:
2285 1917 1384 677 438 268 268 249
6
AfeI AGC'GCT
Cuts at: 2123 2123
Size: 7492
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7295
AflIII A'CryG_T
Cuts at: 1203 6391 7368 1203
Size: 5188 977 1327
Fragments arranged by size:
5188 1327 977
AgeI A'CCGG_T
Cuts at: 6323 6323
Size: 7492
AhdI GACnn_n'nnGTC
Cuts at: 6221 6221
Size: 7492
AleI CACnn'nnGTG
Cuts at: 1166 2111 4568 1166
Size: 945 2457 4090
Fragments arranged by size:
4090 2457 945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7492
AlwNI CAG_nnn'CTG
Cuts at: 2065 2094 2137 5235 6807 2065
Size: 29 43 3098 1572 2750
Fragments arranged by size:
3098 2750 1572 43 29
ApaI G_GGCC'C
Cuts at: 4478 4478
Size: 7492
ApaLI G'TGCA_C
Cuts at: 5549 6705 5549
Size: 1156 6336
ApoI r'AATT_y
Cuts at: 1493 2289 2524 3227 3279 3737 3785 4801 5027
Size: 796 235 703 52 458 48 1016 226
Cuts at: 5027 5179 7288 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1016 796 703 458 235 226
152 52 48
AseI AT'TA_AT
Cuts at: 160 1032 2514 6046 160
Size: 872 1482 3532 1606
Fragments arranged by size:
3532 1606 1482 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
AvaI C'yCGr_G
Cuts at: 1870 1943 2640 2735 4264 4346 4495 4817 1870
Size: 73 697 95 1529 82 149 322 4545
Fragments arranged by size:
4545 1529 697 322 149 95 82 73
BamHI G'GATC_C
Cuts at: 3344 4822 5189 3344
Size: 1478 367 5647
Fragments arranged by size:
5647 1478 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2431 2722 4360 4671 4813
Size: 325 217 627 644 291 1638 311 142
Cuts at: 4813 6268 618
Size: 1455 1842
Fragments arranged by size:
1842 1638 1455 644 627 325 311 291
217 142
BanII G_rGCy'C
Cuts at: 1391 2169 2957 2975 4478 4789 4811 1391
Size: 778 788 18 1503 311 22 4072
Fragments arranged by size:
4072 1503 788 778 311 22 18
BbeI G_GCGC'C
Cuts at: 4364 4364
Size: 7492
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2253 2394 2855 4388 928
Size: 971 354 141 461 1533 4032
Fragments arranged by size:
4032 1533 971 461 354 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5683 5717 1903
Size: 34 3746 34 3678
Fragments arranged by size:
3746 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2929 3735 5384 6594 2929
Size: 806 1649 1210 3827
Fragments arranged by size:
3827 1649 1210 806
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7492
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4349 4358 4514
Size: 107 122 71 631 605 2677 9 156
Cuts at: 4514 6103 136
Size: 1589 1525
Fragments arranged by size:
2677 1589 1525 631 605 156 122 107
71 9
BglII A'GATC_T
Cuts at: 2052 4596 7487 2052
Size: 2544 2891 2057
Fragments arranged by size:
2891 2544 2057
BlpI GC'TnA_GC
Cuts at: 2087 3204 4909 2087
Size: 1117 1705 4670
Fragments arranged by size:
4670 1705 1117
Bme1580I G_kGCm'C
Cuts at: 1790 2436 4478 5553 6709 1790
Size: 646 2042 1075 1156 2573
Fragments arranged by size:
2573 2042 1156 1075 646
BmgBI CAC'GTC
Cuts at: 1318 2113 1318
Size: 795 6697
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2866 3449 5223
Size: 416 53 83 399 24 1449 583 1774
Cuts at: 5223 6181 442
Size: 958 1753
Fragments arranged by size:
1774 1753 1449 958 583 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7492
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2666 2035
Size: 631 6861
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2005 2676 2739 2823 4003 6152 1239
Size: 766 671 63 84 1180 2149 2579
Fragments arranged by size:
2579 2149 1180 766 671 84 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2391 3361 5617 6482 6780 2391
Size: 970 2256 865 298 3103
Fragments arranged by size:
3103 2256 970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2044 2533 3004 6155 882
Size: 617 545 489 471 3151 2219
Fragments arranged by size:
3151 2219 617 545 489 471
BsaAI yAC'GTr
Cuts at: 493 1204 3428 4570 5283 493
Size: 711 2224 1142 713 2702
Fragments arranged by size:
2702 2224 1142 713 711
BsaBI GATnn'nnATC
Cuts at: 3244 3798 4322 4601 7486 3244
Size: 554 524 279 2885 3250
Fragments arranged by size:
3250 2885 554 524 279
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4361 5299 5681 6316
Size: 53 83 186 936 2828 938 382 635
Cuts at: 6316 275
Size: 1451
Fragments arranged by size:
2828 1451 938 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3365 4060 5925 6323 6597 6744 7155 3365
Size: 695 1865 398 274 147 411 3702
Fragments arranged by size:
3702 1865 695 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2821 2851 1178
Size: 30 165 30 1418 30 5819
Fragments arranged by size:
5819 1418 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2513 3342 3489 3548 4091 1186
Size: 907 420 829 147 59 543 4587
Fragments arranged by size:
4587 907 829 543 420 147 59
BseYI C'CCAG_C
Cuts at: 2065 2070 2137 3107 3952 4027 6695 7191 2065
Size: 5 67 970 845 75 2668 496 2366
Fragments arranged by size:
2668 2366 970 845 496 75 67 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2102 4686 1163
Size: 573 366 2584 3969
Fragments arranged by size:
3969 2584 573 366
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4942 5703 6731 1540
Size: 275 186 2941 761 1028 2301
Fragments arranged by size:
2941 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 5553 5638 6709 2169
Size: 3384 85 1071 2952
Fragments arranged by size:
3384 2952 1071 85
BsmI GAATG_Cn'
Cuts at: 5003 5096 5003
Size: 93 7399
BsmBI CGTCTCn'nnnn_
Cuts at: 4547 4547
Size: 7492
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2436 2957 2975 4478 4789 4811
Size: 399 379 267 521 18 1503 311 22
Cuts at: 4811 5553 5638 6709 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1503 1071 742 521 399 379 311
267 85 22 18
BspEI T'CCGG_A
Cuts at: 4060 7155 4060
Size: 3095 4397
BspHI T'CATG_A
Cuts at: 4695 5381 7111 4695
Size: 686 1730 5076
Fragments arranged by size:
5076 1730 686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2394 2419 2458 4847 844
Size: 1550 25 39 2389 3489
Fragments arranged by size:
3489 2389 1550 39 25
BsrBI CCG'CTC
Cuts at: 1394 1823 5379 1394
Size: 429 3556 3507
Fragments arranged by size:
3556 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2889 5987 6161 65
Size: 1183 1641 3098 174 1396
Fragments arranged by size:
3098 1641 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4357 4393 6136 6323 1700
Size: 110 2547 36 1743 187 2869
Fragments arranged by size:
2869 2547 1743 187 110 36
BsrGI T'GTAC_A
Cuts at: 96 2701 96
Size: 2605 4887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7492
BssSI C'ACGA_G
Cuts at: 3192 5552 6564 3192
Size: 2360 1012 4120
Fragments arranged by size:
4120 2360 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7492
BstBI TT'CG_AA
Cuts at: 3225 6296 3225
Size: 3071 4421
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7492
BstYI r'GATC_y
Cuts at: 1972 2052 2673 3217 3344 3760 4596 4602 4822
Size: 80 621 544 127 416 836 6 220
Cuts at: 4822 5189 5574 5591 7043 7129 7141 7487 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 836 621 544 416 385 367
346 220 127 86 80 17 12 6
BtgI C'CryG_G
Cuts at: 513 1065 3558 4470 513
Size: 552 2493 912 3535
Fragments arranged by size:
3535 2493 912 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4677 487
Size: 683 56 3451 3302
Fragments arranged by size:
3451 3302 683 56
BtsI GCAGTG_nn'
Cuts at: 4727 5008 5802 5822 4727
Size: 281 794 20 6397
Fragments arranged by size:
6397 794 281 20
ClaI AT'CG_AT
Cuts at: 1554 3177 1554
Size: 1623 5869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3951 3986 529
Size: 35 3387 35 4035
Fragments arranged by size:
4035 3387 35 35
DraI TTT'AAA
Cuts at: 3775 4796 3775
Size: 1021 6471
DraIII CAC_nnn'GTG
Cuts at: 3050 3526 3050
Size: 476 7016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4319 6499 809
Size: 507 3003 2180 1802
Fragments arranged by size:
3003 2180 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2173 4306 4939
Size: 54 1425 50 275 18 343 2133 633
Cuts at: 4939 5828 7457 8
Size: 889 1629 43
Fragments arranged by size:
2133 1629 1425 889 633 343 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4939 1537
Size: 275 3127 4090
Fragments arranged by size:
4090 3127 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2418 3487 3747 4178 4193 4707 4766 5422
Size: 1065 1069 260 431 15 514 59 656
Cuts at: 5422 1353
Size: 3423
Fragments arranged by size:
3423 1069 1065 656 514 431 260 59
15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6079 6453 6599 387
Size: 1064 138 4490 374 146 1280
Fragments arranged by size:
4490 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2167 2167
Size: 7492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2309 2577 2583 2676 2739 2823
Size: 393 373 304 268 6 93 63 84
Cuts at: 2823 3021 3270 4003 5555 6152 6939 7207 1239
Size: 198 249 733 1552 597 787 268 1524
Fragments arranged by size:
1552 1524 787 733 597 393 373 304
268 268 249 198 93 84 63 6
EcoO109I rG'GnC_Cy
Cuts at: 4024 4475 4499 4024
Size: 451 24 7017
Fragments arranged by size:
7017 451 24
EcoRI G'AATT_C
Cuts at: 4801 5179 4801
Size: 378 7114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3842 3842
Size: 7492
FspI TGC'GCA
Cuts at: 4538 5998 4538
Size: 1460 6032
HaeII r_GCGC'y
Cuts at: 1678 2066 2094 2125 4364 6639 1678
Size: 388 28 31 2239 2275 2531
Fragments arranged by size:
2531 2275 2239 388 31 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4275 4307 4765 4797 6133
Size: 32 135 32 2703 32 458 32 1336
Cuts at: 6133 6165 6207 6239 1373
Size: 32 42 32 2626
Fragments arranged by size:
2703 2626 1336 458 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4836 5082 669
Size: 988 281 2898 246 3079
Fragments arranged by size:
3079 2898 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2208 3309 748
Size: 1460 1101 4931
Fragments arranged by size:
4931 1460 1101
HpaI GTT'AAC
Cuts at: 5082 5082
Size: 7492
KasI G'GCGC_C
Cuts at: 4360 4360
Size: 7492
KpnI G_GTAC'C
Cuts at: 4675 4817 4675
Size: 142 7350
MfeI C'AATT_G
Cuts at: 5091 5091
Size: 7492
MluI A'CGCG_T
Cuts at: 7368 7368
Size: 7492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2869 4669 6606 6790 1305
Size: 1564 1800 1937 184 2007
Fragments arranged by size:
2007 1937 1800 1564 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4308 10
Size: 54 1425 343 2476 3194
Fragments arranged by size:
3194 2476 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 4227 4568 5450 5809 5968 518
Size: 648 945 2116 341 882 359 159 2042
Fragments arranged by size:
2116 2042 945 882 648 359 341 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2218 4447 4453 4472 4908 5585 6733
Size: 531 81 2229 6 19 436 677 1148
Cuts at: 6733 6978 1606
Size: 245 2120
Fragments arranged by size:
2229 2120 1148 677 531 436 245 81
19 6
NaeI GCC'GGC
Cuts at: 4359 4395 4359
Size: 36 7456
NarI GG'CG_CC
Cuts at: 4361 4361
Size: 7492
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6940
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7492
NgoMIV G'CCGG_C
Cuts at: 4357 4393 4357
Size: 36 7456
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7492
NotI GC'GGCC_GC
Cuts at: 4939 4939
Size: 7492
NspI r_CATG'y
Cuts at: 1132 1229 4850 6395 1132
Size: 97 3621 1545 2229
Fragments arranged by size:
3621 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4610 1348
Size: 3262 4230
PciI A'CATG_T
Cuts at: 6391 6391
Size: 7492
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2065 2221 1108
Size: 957 156 6379
Fragments arranged by size:
6379 957 156
PfoI T'CCnGG_A
Cuts at: 2839 2839
Size: 7492
PmeI GTTT'AAAC
Cuts at: 4796 4796
Size: 7492
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5561 5593 7074 7106 5561
Size: 32 1481 32 5947
Fragments arranged by size:
5947 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4024 4499 4024
Size: 475 7017
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7492
PsiI TTA'TAA
Cuts at: 5062 5062
Size: 7492
PspOMI G'GGCC_C
Cuts at: 4474 4474
Size: 7492
PstI C_TGCA'G
Cuts at: 830 2121 2133 4844 830
Size: 1291 12 2711 3478
Fragments arranged by size:
3478 2711 1291 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7492
PvuII CAG'CTG
Cuts at: 2137 2218 2137
Size: 81 7411
SacI G_AGCT'C
Cuts at: 2169 2169
Size: 7492
SacII CC_GC'GG
Cuts at: 4473 4473
Size: 7492
SalI G'TCGA_C
Cuts at: 1936 4834 1936
Size: 2898 4594
SanDI GG'GwC_CC
Cuts at: 4024 4499 4024
Size: 475 7017
SapI GCTCTTCn'nnn_
Cuts at: 2418 2418
Size: 7492
SbfI CC_TGCA'GG
Cuts at: 4844 4844
Size: 7492
ScaI AGT'ACT
Cuts at: 5740 7452 5740
Size: 1712 5780
SfcI C'TryA_G
Cuts at: 826 1045 2117 2129 4840 5975 6656 6847 826
Size: 219 1072 12 2711 1135 681 191 1471
Fragments arranged by size:
2711 1471 1135 1072 681 219 191 12
SfoI GGC'GCC
Cuts at: 4362 4362
Size: 7492
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7492
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7492
SmaI CCC'GGG
Cuts at: 1872 4497 4819 1872
Size: 2625 322 4545
Fragments arranged by size:
4545 2625 322
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2406 2640 3376 5596 6497 6759
Size: 197 926 463 234 736 2220 901 262
Cuts at: 6759 820
Size: 1553
Fragments arranged by size:
2220 1553 926 901 736 463 262 234
197
SnaBI TAC'GTA
Cuts at: 493 3428 5283 493
Size: 2935 1855 2702
Fragments arranged by size:
2935 2702 1855
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7492
SphI G_CATG'C
Cuts at: 1132 4850 1132
Size: 3718 3774
SspI AAT'ATT
Cuts at: 5 52 5416 5
Size: 47 5364 2081
Fragments arranged by size:
5364 2081 47
StuI AGG'CCT
Cuts at: 1517 3170 1517
Size: 1653 5839
StyI C'CwwG_G
Cuts at: 513 1065 2475 2976 4931 513
Size: 552 1410 501 1955 3074
Fragments arranged by size:
3074 1955 1410 552 501
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2407 4602 4638 5519 5536 5689 5874
Size: 1255 827 2195 36 881 17 153 185
Cuts at: 5874 6319 325
Size: 445 1498
Fragments arranged by size:
2195 1498 1255 881 827 445 185 153
36 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2320 2701 2711
Size: 274 80 33 51 1443 343 381 10
Cuts at: 2711 3296 5738 7450 96
Size: 585 2442 1712 138
Fragments arranged by size:
2442 1712 1443 585 381 343 274 138
80 51 33 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7452 7484 7452
Size: 32 7460
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7492
XbaI T'CTAG_A
Cuts at: 4828 4828
Size: 7492
XhoI C'TCGA_G
Cuts at: 1943 2640 1943
Size: 697 6795
XmaI C'CCGG_G
Cuts at: 1870 4495 4817 1870
Size: 2625 322 4545
Fragments arranged by size:
4545 2625 322
XmnI GAAnn'nnTTC
Cuts at: 2373 3329 3448 5621 2373
Size: 956 119 2173 4244
Fragments arranged by size:
4244 2173 956 119
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5300 276
Size: 53 83 186 936 3766 2468
Fragments arranged by size:
3766 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI
SrfI SwaI XcmI