Restriction map for
pFN21AE1288
(Circular) (Six-base) MAPSORT of: pFN21AE1288.seq Check: 3997 from: 1 to: 7411
pFN21AE1288 7411 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5221 278
Size: 53 83 186 936 3685 2468
Fragments arranged by size:
3685 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4570 4754 1937
Size: 2633 184 4594
Fragments arranged by size:
4594 2633 184
Acc65I G'GTAC_C
Cuts at: 4732 4732
Size: 7411
AclI AA'CG_TT
Cuts at: 1563 5538 5911 1563
Size: 3975 373 3063
Fragments arranged by size:
3975 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2133 2259 2400 4038 4403 5474 6858 7126
Size: 501 126 141 1638 365 1071 1384 268
Cuts at: 7126 1632
Size: 1917
Fragments arranged by size:
1917 1638 1384 1071 501 365 268 141
126
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7214
AflIII A'CryG_T
Cuts at: 1203 2875 4453 6310 7287 1203
Size: 1672 1578 1857 977 1327
Fragments arranged by size:
1857 1672 1578 1327 977
AgeI A'CCGG_T
Cuts at: 6242 6242
Size: 7411
AhdI GACnn_n'nnGTC
Cuts at: 2428 6140 2428
Size: 3712 3699
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7411
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7411
AlwNI CAG_nnn'CTG
Cuts at: 2171 2336 2723 5154 6726 2171
Size: 165 387 2431 1572 2856
Fragments arranged by size:
2856 2431 1572 387 165
ApaI G_GGCC'C
Cuts at: 2913 2913
Size: 7411
ApaLI G'TGCA_C
Cuts at: 5468 6624 5468
Size: 1156 6255
ApoI r'AATT_y
Cuts at: 1493 3300 3730 3743 4363 4720 4946 5098 7207
Size: 1807 430 13 620 357 226 152 2109
Cuts at: 7207 1493
Size: 1697
Fragments arranged by size:
2109 1807 1697 620 430 357 226 152
13
AseI AT'TA_AT
Cuts at: 160 1032 5965 160
Size: 872 4933 1606
Fragments arranged by size:
4933 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
AvaI C'yCGr_G
Cuts at: 1870 1943 2232 3542 4736 1870
Size: 73 289 1310 1194 4545
Fragments arranged by size:
4545 1310 1194 289 73
AvrII C'CTAG_G
Cuts at: 2252 2252
Size: 7411
BamHI G'GATC_C
Cuts at: 4741 5108 4741
Size: 367 7044
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2800 3591 4664 4732 6187
Size: 325 217 627 1013 791 1073 68 1455
Cuts at: 6187 618
Size: 1842
Fragments arranged by size:
1842 1455 1073 1013 791 627 325 217
68
BanII G_rGCy'C
Cuts at: 1391 2913 4730 1391
Size: 1522 1817 4072
Fragments arranged by size:
4072 1817 1522
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4173 928
Size: 971 2274 4166
Fragments arranged by size:
4166 2274 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5602 5636 1903
Size: 34 3665 34 3678
Fragments arranged by size:
3678 3665 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2728 2734 5303 6513 2728
Size: 6 2569 1210 3626
Fragments arranged by size:
3626 2569 1210 6
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7411
BfrBI ATG'CAT
Cuts at: 4328 4328
Size: 7411
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6022 136
Size: 107 122 71 631 605 4350 1525
Fragments arranged by size:
4350 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2375 7406 2375
Size: 5031 2380
BlpI GC'TnA_GC
Cuts at: 3549 4828 3549
Size: 1279 6132
Bme1580I G_kGCm'C
Cuts at: 1790 2913 4667 5472 6628 1790
Size: 1123 1754 805 1156 2573
Fragments arranged by size:
2573 1754 1156 1123 805
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5142 6100 442
Size: 416 53 83 399 24 3725 958 1753
Fragments arranged by size:
3725 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3623 4512 6071 1239
Size: 2384 889 1559 2579
Fragments arranged by size:
2579 2384 1559 889
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2118 3053 3102 3543 3877 5536 6401 6699 2118
Size: 935 49 441 334 1659 865 298 2830
Fragments arranged by size:
2830 1659 935 865 441 334 298 49
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2155 6074 882
Size: 617 656 3919 2219
Fragments arranged by size:
3919 2219 656 617
BsaAI yAC'GTr
Cuts at: 493 1204 5202 493
Size: 711 3998 2702
Fragments arranged by size:
3998 2702 711
BsaBI GATnn'nnATC
Cuts at: 2146 3453 7405 2146
Size: 1307 3952 2152
Fragments arranged by size:
3952 2152 1307
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5218 5600 6235 275
Size: 53 83 186 936 3685 382 635 1451
Fragments arranged by size:
3685 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3050 5844 6242 6516 6663 7074 3050
Size: 2794 398 274 147 411 3387
Fragments arranged by size:
3387 2794 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7186
Fragments arranged by size:
7186 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2458 2963 3236 1186
Size: 1272 505 273 5361
Fragments arranged by size:
5361 1272 505 273
BseYI C'CCAG_C
Cuts at: 6614 7110 6614
Size: 496 6915
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2843 1163
Size: 573 1107 5731
Fragments arranged by size:
5731 1107 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4861 5622 6650 1540
Size: 275 186 2860 761 1028 2301
Fragments arranged by size:
2860 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5472 5557 6628 5472
Size: 85 1071 6255
Fragments arranged by size:
6255 1071 85
BsmI GAATG_Cn'
Cuts at: 4131 4922 5015 4131
Size: 791 93 6527
Fragments arranged by size:
6527 791 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2913 4667 4730 5472 5557 6628 1391
Size: 399 1123 1754 63 742 85 1071 2174
Fragments arranged by size:
2174 1754 1123 1071 742 399 85 63
BspEI T'CCGG_A
Cuts at: 3050 7074 3050
Size: 4024 3387
BspHI T'CATG_A
Cuts at: 4239 5300 7030 4239
Size: 1061 1730 4620
Fragments arranged by size:
4620 1730 1061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2815 3811 4766 844
Size: 1971 996 955 3489
Fragments arranged by size:
3489 1971 996 955
BsrBI CCG'CTC
Cuts at: 1394 1823 5298 1394
Size: 429 3475 3507
Fragments arranged by size:
3507 3475 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2100 4510 5906 6080 65
Size: 1183 852 2410 1396 174 1396
Fragments arranged by size:
2410 1396 1396 1183 852 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6055 6242 1700
Size: 110 4245 187 2869
Fragments arranged by size:
4245 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7411
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7411
BssSI C'ACGA_G
Cuts at: 2025 5471 6483 2025
Size: 3446 1012 2953
Fragments arranged by size:
3446 2953 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7411
BstBI TT'CG_AA
Cuts at: 6215 6215
Size: 7411
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7411
BstXI CCAn_nnnn'nTGG
Cuts at: 3725 3725
Size: 7411
BstYI r'GATC_y
Cuts at: 1972 2375 3826 4741 5108 5493 5510 6962 7048
Size: 403 1451 915 367 385 17 1452 86
Cuts at: 7048 7060 7406 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1451 915 403 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5920
Fragments arranged by size:
5920 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6672
Fragments arranged by size:
6672 683 56
BtsI GCAGTG_nn'
Cuts at: 2983 2999 4311 4927 5721 5741 2983
Size: 16 1312 616 794 20 4653
Fragments arranged by size:
4653 1312 794 616 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7376
DraI TTT'AAA
Cuts at: 2217 2352 3748 4396 4715 2217
Size: 135 1396 648 319 4913
Fragments arranged by size:
4913 1396 648 319 135
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3816 6418 809
Size: 507 2500 2602 1802
Fragments arranged by size:
2602 2500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4858 5747 7376
Size: 54 1425 50 275 18 3028 889 1629
Cuts at: 7376 8
Size: 43
Fragments arranged by size:
3028 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4858 1537
Size: 275 3046 4090
Fragments arranged by size:
4090 3046 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2128 2153 3435 3766 5341 1353
Size: 775 25 1282 331 1575 3423
Fragments arranged by size:
3423 1575 1282 775 331 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5998 6372 6518 387
Size: 1064 138 4409 374 146 1280
Fragments arranged by size:
4409 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2259 2400 3623 4038 4403 4512
Size: 393 501 126 141 1223 415 365 109
Cuts at: 4512 5474 6071 6858 7126 1239
Size: 962 597 787 268 1524
Fragments arranged by size:
1524 1223 962 787 597 501 415 393
365 268 141 126 109
EcoNI CCTnn'n_nnAGG
Cuts at: 3168 3168
Size: 7411
EcoO109I rG'GnC_Cy
Cuts at: 2909 2910 2909
Size: 1 7410
EcoRI G'AATT_C
Cuts at: 4720 5098 4720
Size: 378 7033
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2582 4237 2582
Size: 1655 5756
FspI TGC'GCA
Cuts at: 2106 5917 2106
Size: 3811 3600
HaeII r_GCGC'y
Cuts at: 1678 6558 1678
Size: 4880 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2414 2446 3409 3441 3830
Size: 32 135 32 842 32 963 32 389
Cuts at: 3830 3862 3865 3897 4271 4303 6052 6084 6126
Size: 32 3 32 374 32 1749 32 42
Cuts at: 6126 6158 1373
Size: 32 2626
Fragments arranged by size:
2626 1749 963 842 389 374 135 42
32 32 32 32 32 32 32 32
32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 4755 5001 669
Size: 988 281 2817 246 3079
Fragments arranged by size:
3079 2817 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7411
HpaI GTT'AAC
Cuts at: 5001 5001
Size: 7411
KpnI G_GTAC'C
Cuts at: 4736 4736
Size: 7411
MfeI C'AATT_G
Cuts at: 3686 5010 3686
Size: 1324 6087
MluI A'CGCG_T
Cuts at: 7287 7287
Size: 7411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2814 6525 6709 1305
Size: 1509 3711 184 2007
Fragments arranged by size:
3711 2007 1509 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5589
Fragments arranged by size:
5589 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4238 5369 5728 5887 518
Size: 648 3072 1131 359 159 2042
Fragments arranged by size:
3072 2042 1131 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2636 2887 4827 5504 6652 6897 1606
Size: 1030 251 1940 677 1148 245 2120
Fragments arranged by size:
2120 1940 1148 1030 677 251 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5920
Fragments arranged by size:
5920 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7411
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7411
NotI GC'GGCC_GC
Cuts at: 4858 4858
Size: 7411
NsiI A_TGCA'T
Cuts at: 4330 4330
Size: 7411
NspI r_CATG'y
Cuts at: 1132 1229 2879 4457 4769 6314 1132
Size: 97 1650 1578 312 1545 2229
Fragments arranged by size:
2229 1650 1578 1545 312 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7411
PciI A'CATG_T
Cuts at: 2875 4453 6310 2875
Size: 1578 1857 3976
Fragments arranged by size:
3976 1857 1578
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7411
PmeI GTTT'AAAC
Cuts at: 4715 4715
Size: 7411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3984 4016 5480 5512 6993 7025 3984
Size: 32 1464 32 1481 32 4370
Fragments arranged by size:
4370 1481 1464 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3027 1613
Size: 1414 5997
PsiI TTA'TAA
Cuts at: 2642 4981 2642
Size: 2339 5072
PspOMI G'GGCC_C
Cuts at: 2909 2909
Size: 7411
PspXI vC'TCGA_Gb
Cuts at: 3542 3542
Size: 7411
PstI C_TGCA'G
Cuts at: 830 2578 4763 830
Size: 1748 2185 3478
Fragments arranged by size:
3478 2185 1748
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7411
PvuII CAG'CTG
Cuts at: 2636 2887 2636
Size: 251 7160
SalI G'TCGA_C
Cuts at: 1936 4753 1936
Size: 2817 4594
SbfI CC_TGCA'GG
Cuts at: 4763 4763
Size: 7411
ScaI AGT'ACT
Cuts at: 5659 7371 5659
Size: 1712 5699
SfcI C'TryA_G
Cuts at: 826 1045 2304 2574 2904 4759 5894 6575 6766
Size: 219 1259 270 330 1855 1135 681 191
Cuts at: 6766 826
Size: 1471
Fragments arranged by size:
1855 1471 1259 1135 681 330 270 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7411
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7411
SmaI CCC'GGG
Cuts at: 1872 4738 1872
Size: 2866 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2133 3032 3117 3542 3558 3892
Size: 197 926 190 899 85 425 16 334
Cuts at: 3892 5515 6416 6678 820
Size: 1623 901 262 1553
Fragments arranged by size:
1623 1553 926 901 899 425 334 262
197 190 85 16
SnaBI TAC'GTA
Cuts at: 493 5202 493
Size: 4709 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7411
SphI G_CATG'C
Cuts at: 1132 4769 1132
Size: 3637 3774
SspI AAT'ATT
Cuts at: 5 52 3391 5335 5
Size: 47 3339 1944 2081
Fragments arranged by size:
3339 2081 1944 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7411
StyI C'CwwG_G
Cuts at: 513 1065 2004 2252 3724 4850 513
Size: 552 939 248 1472 1126 3074
Fragments arranged by size:
3074 1472 1126 939 552 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4299 5438 5455 5608 5793 6238 325
Size: 1255 2719 1139 17 153 185 445 1498
Fragments arranged by size:
2719 1498 1255 1139 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2447 3538 4096
Size: 274 80 33 51 1443 470 1091 558
Cuts at: 4096 5657 7369 96
Size: 1561 1712 138
Fragments arranged by size:
1712 1561 1443 1091 558 470 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3313 3345 7371 7403 3313
Size: 32 4026 32 3321
Fragments arranged by size:
4026 3321 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7411
XbaI T'CTAG_A
Cuts at: 3076 4747 3076
Size: 1671 5740
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3016 3625 3016
Size: 609 6802
XhoI C'TCGA_G
Cuts at: 1943 3542 1943
Size: 1599 5812
XmaI C'CCGG_G
Cuts at: 1870 4736 1870
Size: 2866 4545
XmnI GAAnn'nnTTC
Cuts at: 4106 4344 5540 4106
Size: 238 1196 5977
Fragments arranged by size:
5977 1196 238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5219 276
Size: 53 83 186 936 3685 2468
Fragments arranged by size:
3685 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI