Restriction map for
pFN21AE1273
(Circular) (Six-base) MAPSORT of: pFN21AE1273.seq Check: 5366 from: 1 to: 6793
pFN21AE1273 6793 bp
With 182 enzymes: SgfI *
June 21, 2019 12:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3253 3352 3253
Size: 99 6694
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4603 278
Size: 53 83 186 936 3067 2468
Fragments arranged by size:
3067 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2529 4136 1937
Size: 592 1607 4594
Fragments arranged by size:
4594 1607 592
Acc65I G'GTAC_C
Cuts at: 4114 4114
Size: 6793
AclI AA'CG_TT
Cuts at: 1563 4920 5293 1563
Size: 3357 373 3063
Fragments arranged by size:
3357 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2425 3174 3903 3936 4856 6240 6508 1632
Size: 793 749 729 33 920 1384 268 1917
Fragments arranged by size:
1917 1384 920 793 749 729 268 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6596
AflIII A'CryG_T
Cuts at: 1203 5692 6669 1203
Size: 4489 977 1327
Fragments arranged by size:
4489 1327 977
AgeI A'CCGG_T
Cuts at: 5624 5624
Size: 6793
AhdI GACnn_n'nnGTC
Cuts at: 5522 5522
Size: 6793
AleI CACnn'nnGTG
Cuts at: 1166 2667 3171 1166
Size: 1501 504 4788
Fragments arranged by size:
4788 1501 504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3189 1184
Size: 2005 4788
AlwNI CAG_nnn'CTG
Cuts at: 2053 2891 3250 3283 3319 3617 4064 4536 6108
Size: 838 359 33 36 298 447 472 1572
Cuts at: 6108 2053
Size: 2738
Fragments arranged by size:
2738 1572 838 472 447 359 298 36
33
ApaLI G'TGCA_C
Cuts at: 2340 2670 4850 6006 2340
Size: 330 2180 1156 3127
Fragments arranged by size:
3127 2180 1156 330
ApoI r'AATT_y
Cuts at: 1493 2617 2942 3209 3686 3718 4102 4328 4480
Size: 1124 325 267 477 32 384 226 152
Cuts at: 4480 6589 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1124 477 384 325 267 226
152 32
AseI AT'TA_AT
Cuts at: 160 1032 5347 160
Size: 872 4315 1606
Fragments arranged by size:
4315 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
AvaI C'yCGr_G
Cuts at: 1870 1943 2832 3597 4118 1870
Size: 73 889 765 521 4545
Fragments arranged by size:
4545 889 765 521 73
BamHI G'GATC_C
Cuts at: 4123 4490 4123
Size: 367 6426
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4114 5569 618
Size: 325 217 627 2327 1455 1842
Fragments arranged by size:
2327 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4112 1391
Size: 2721 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2442 2664 2910 4053 928
Size: 971 543 222 246 1143 3668
Fragments arranged by size:
3668 1143 971 543 246 222
BbvCI CC'TCA_GC
Cuts at: 3177 3177
Size: 6793
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3965 3999 4984 5018 1903
Size: 34 2028 34 985 34 3678
Fragments arranged by size:
3678 2028 985 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4685 5895 4685
Size: 1210 5583
BclI T'GATC_A
Cuts at: 1548 3204 1548
Size: 1656 5137
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4005 5404 136
Size: 107 122 71 631 605 2333 1399 1525
Fragments arranged by size:
2333 1525 1399 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6788 6788
Size: 6793
BlpI GC'TnA_GC
Cuts at: 3396 4210 3396
Size: 814 5979
Bme1580I G_kGCm'C
Cuts at: 1790 2344 2674 3168 3330 4854 6010 1790
Size: 554 330 494 162 1524 1156 2573
Fragments arranged by size:
2573 1524 1156 554 494 330 162
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6793
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2176 4524 5482
Size: 416 53 83 399 24 759 2348 958
Cuts at: 5482 442
Size: 1753
Fragments arranged by size:
2348 1753 958 759 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6793
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 6793
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 3053 3923 5453 1239
Size: 1229 585 870 1530 2579
Fragments arranged by size:
2579 1530 1229 870 585
Bpu10I CC'TnA_GC
Cuts at: 3177 3177
Size: 6793
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2132 2676 3095 3422 4918 5783 6081 2132
Size: 544 419 327 1496 865 298 2844
Fragments arranged by size:
2844 1496 865 544 419 327 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3914 5456 882
Size: 617 2415 1542 2219
Fragments arranged by size:
2415 2219 1542 617
BsaAI yAC'GTr
Cuts at: 493 1204 4584 493
Size: 711 3380 2702
Fragments arranged by size:
3380 2702 711
BsaBI GATnn'nnATC
Cuts at: 6787 6787
Size: 6793
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4600 4982 5617 275
Size: 53 83 186 936 3067 382 635 1451
Fragments arranged by size:
3067 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5226 5624 5898 6045 6456 5226
Size: 398 274 147 411 5563
Fragments arranged by size:
5563 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6568
Fragments arranged by size:
6568 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 3011 3029 3050 3092 3116 3284 3419
Size: 1210 615 18 21 42 24 168 135
Cuts at: 3419 3875 4040 4052 1186
Size: 456 165 12 3927
Fragments arranged by size:
3927 1210 615 456 168 165 135 42
24 21 18 12
BseYI C'CCAG_C
Cuts at: 3276 3652 5996 6492 3276
Size: 376 2344 496 3577
Fragments arranged by size:
3577 2344 496 376
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2667 2861 3278 1163
Size: 573 931 194 417 4678
Fragments arranged by size:
4678 931 573 417 194
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3416 4243 5004 6032 1540
Size: 275 186 1415 827 761 1028 2301
Fragments arranged by size:
2301 1415 1028 827 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 2344 2674 3021 4854 4939 6010 2024
Size: 320 330 347 1833 85 1071 2807
Fragments arranged by size:
2807 1833 1071 347 330 320 85
BsmI GAATG_Cn'
Cuts at: 4304 4397 4304
Size: 93 6700
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2344 2674 3021 3168 3330 4112
Size: 399 234 320 330 347 147 162 782
Cuts at: 4112 4854 4939 6010 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 782 742 399 347 330 320
234 162 147 85
BspEI T'CCGG_A
Cuts at: 6456 6456
Size: 6793
BspHI T'CATG_A
Cuts at: 4682 6412 4682
Size: 1730 5063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3043 3253 3352 4148 844
Size: 2199 210 99 796 3489
Fragments arranged by size:
3489 2199 796 210 99
BsrBI CCG'CTC
Cuts at: 1394 1823 4680 1394
Size: 429 2857 3507
Fragments arranged by size:
3507 2857 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5288 5462 65
Size: 1183 4040 174 1396
Fragments arranged by size:
4040 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3411 5437 5624 1700
Size: 110 1601 2026 187 2869
Fragments arranged by size:
2869 2026 1601 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6793
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6793
BssSI C'ACGA_G
Cuts at: 2378 3582 4853 5865 2378
Size: 1204 1271 1012 3306
Fragments arranged by size:
3306 1271 1204 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2678 3319 794
Size: 1884 641 4268
Fragments arranged by size:
4268 1884 641
BstBI TT'CG_AA
Cuts at: 2746 5597 2746
Size: 2851 3942
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6793
BstXI CCAn_nnnn'nTGG
Cuts at: 3157 3157
Size: 6793
BstYI r'GATC_y
Cuts at: 1972 3559 4123 4490 4875 4892 6344 6430 6442
Size: 1587 564 367 385 17 1452 86 12
Cuts at: 6442 6788 1972
Size: 346 1977
Fragments arranged by size:
1977 1587 1452 564 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2530 2530
Size: 6793
Bsu36I CC'TnA_GG
Cuts at: 2994 2994
Size: 6793
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5302
Fragments arranged by size:
5302 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2108 2405 487
Size: 683 56 882 297 4875
Fragments arranged by size:
4875 882 683 297 56
BtsI GCAGTG_nn'
Cuts at: 3261 4309 5103 5123 3261
Size: 1048 794 20 4931
Fragments arranged by size:
4931 1048 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6793
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6758
DraI TTT'AAA
Cuts at: 4097 4097
Size: 6793
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5800 809
Size: 507 4484 1802
Fragments arranged by size:
4484 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3413 4240 5129
Size: 54 1425 50 275 18 1583 827 889
Cuts at: 5129 6758 8
Size: 1629 43
Fragments arranged by size:
1629 1583 1425 889 827 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3413 4240 1537
Size: 275 1601 827 4090
Fragments arranged by size:
4090 1601 827 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 2538 2890 3006 3732 3843 3852 3879
Size: 1053 132 352 116 726 111 9 27
Cuts at: 3879 3912 3966 4029 4075 4723 1353
Size: 33 54 63 46 648 3423
Fragments arranged by size:
3423 1053 726 648 352 132 116 111
63 54 46 33 27 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2167 5380 5754 5900 387
Size: 1064 138 578 3213 374 146 1280
Fragments arranged by size:
3213 1280 1064 578 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2425 2468 3053 3174 3903 3923 3936
Size: 393 793 43 585 121 729 20 13
Cuts at: 3936 4856 5453 6240 6508 1239
Size: 920 597 787 268 1524
Fragments arranged by size:
1524 920 793 787 729 597 585 393
268 121 43 20 13
EcoNI CCTnn'n_nnAGG
Cuts at: 3035 3035
Size: 6793
EcoO109I rG'GnC_Cy
Cuts at: 3028 3593 3028
Size: 565 6228
EcoRI G'AATT_C
Cuts at: 4102 4480 4102
Size: 378 6415
EcoRV GAT'ATC
Cuts at: 3472 3472
Size: 6793
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2258 2288 2258
Size: 30 6763
FseI GG_CCGG'CC
Cuts at: 3415 3415
Size: 6793
FspI TGC'GCA
Cuts at: 5299 5299
Size: 6793
HaeII r_GCGC'y
Cuts at: 1678 2147 5940 1678
Size: 469 3793 2531
Fragments arranged by size:
3793 2531 469
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3599 3631 5434 5466 5508
Size: 32 135 32 2027 32 1803 32 42
Cuts at: 5508 5540 1373
Size: 32 2626
Fragments arranged by size:
2626 2027 1803 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4137 4383 669
Size: 988 281 2199 246 3079
Fragments arranged by size:
3079 2199 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3300 748
Size: 2552 4241
HpaI GTT'AAC
Cuts at: 4383 4383
Size: 6793
KpnI G_GTAC'C
Cuts at: 4118 4118
Size: 6793
MfeI C'AATT_G
Cuts at: 4392 4392
Size: 6793
MluI A'CGCG_T
Cuts at: 6669 6669
Size: 6793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2377 2992 3216 5907 6091 1305
Size: 1072 615 224 2691 184 2007
Fragments arranged by size:
2691 2007 1072 615 224 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4971
Fragments arranged by size:
4971 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2667 3171 3453 3543 4751 5110 5269
Size: 648 1501 504 282 90 1208 359 159
Cuts at: 5269 518
Size: 2042
Fragments arranged by size:
2042 1501 1208 648 504 359 282 159
90
MspA1I CmG'CkG
Cuts at: 1606 2122 3283 3635 4209 4886 6034 6279 1606
Size: 516 1161 352 574 677 1148 245 2120
Fragments arranged by size:
2120 1161 1148 677 574 516 352 245
NaeI GCC'GGC
Cuts at: 3413 3413
Size: 6793
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5302
Fragments arranged by size:
5302 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6793
NgoMIV G'CCGG_C
Cuts at: 3411 3411
Size: 6793
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6793
NotI GC'GGCC_GC
Cuts at: 4240 4240
Size: 6793
NspI r_CATG'y
Cuts at: 1132 1229 4151 5696 1132
Size: 97 2922 1545 2229
Fragments arranged by size:
2922 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6793
PciI A'CATG_T
Cuts at: 5692 5692
Size: 6793
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6793
PfoI T'CCnGG_A
Cuts at: 2890 3156 2890
Size: 266 6527
PmeI GTTT'AAAC
Cuts at: 4097 4097
Size: 6793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4862 4894 6375 6407 4862
Size: 32 1481 32 5248
Fragments arranged by size:
5248 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3593 3593
Size: 6793
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6793
PsiI TTA'TAA
Cuts at: 2280 4363 2280
Size: 2083 4710
PstI C_TGCA'G
Cuts at: 830 2115 3324 3387 4060 4145 830
Size: 1285 1209 63 673 85 3478
Fragments arranged by size:
3478 1285 1209 673 85 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6793
PvuII CAG'CTG
Cuts at: 2122 3283 3635 2122
Size: 1161 352 5280
Fragments arranged by size:
5280 1161 352
SalI G'TCGA_C
Cuts at: 1936 4135 1936
Size: 2199 4594
SapI GCTCTTCn'nnn_
Cuts at: 2890 3732 3966 4075 2890
Size: 842 234 109 5608
Fragments arranged by size:
5608 842 234 109
SbfI CC_TGCA'GG
Cuts at: 4145 4145
Size: 6793
ScaI AGT'ACT
Cuts at: 5041 6753 5041
Size: 1712 5081
SfcI C'TryA_G
Cuts at: 826 1045 2111 3320 3383 3636 4056 4141 5276
Size: 219 1066 1209 63 253 420 85 1135
Cuts at: 5276 5957 6148 826
Size: 681 191 1471
Fragments arranged by size:
1471 1209 1135 1066 681 420 253 219
191 85 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6793
SmaI CCC'GGG
Cuts at: 1872 4120 1872
Size: 2248 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 2691 3074 3401 4897 5798
Size: 197 926 204 544 383 327 1496 901
Cuts at: 5798 6060 820
Size: 262 1553
Fragments arranged by size:
1553 1496 926 901 544 383 327 262
204 197
SnaBI TAC'GTA
Cuts at: 493 4584 493
Size: 4091 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6793
SphI G_CATG'C
Cuts at: 1132 4151 1132
Size: 3019 3774
SspI AAT'ATT
Cuts at: 5 52 4717 5
Size: 47 4665 2081
Fragments arranged by size:
4665 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6793
StyI C'CwwG_G
Cuts at: 513 1065 2004 3502 4232 513
Size: 552 939 1498 730 3074
Fragments arranged by size:
3074 1498 939 730 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2942 4820 4837 4990 5175 5620 325
Size: 1255 1362 1878 17 153 185 445 1498
Fragments arranged by size:
1878 1498 1362 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5039 6751 96
Size: 274 80 33 51 1443 3062 1712 138
Fragments arranged by size:
3062 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2153 2185 2334 2366 3101 3133 6753 6785 2153
Size: 32 149 32 735 32 3620 32 2161
Fragments arranged by size:
3620 2161 735 149 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6793
XbaI T'CTAG_A
Cuts at: 4129 4129
Size: 6793
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6793
XmaI C'CCGG_G
Cuts at: 1870 4118 1870
Size: 2248 4545
XmnI GAAnn'nnTTC
Cuts at: 2268 2886 4922 2268
Size: 618 2036 4139
Fragments arranged by size:
4139 2036 618
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4601 276
Size: 53 83 186 936 3067 2468
Fragments arranged by size:
3067 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI DraIII EcoICRI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI