Restriction map for
pFN21AE1260
(Circular) (Six-base) MAPSORT of: pFN21AE1260.seq Check: 3368 from: 1 to: 6490
pFN21AE1260 6490 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3833 1937
Size: 1896 4594
Acc65I G'GTAC_C
Cuts at: 3811 3811
Size: 6490
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2138 3528 4553 5937 6205 1632
Size: 398 108 1390 1025 1384 268 1917
Fragments arranged by size:
1917 1390 1384 1025 398 268 108
AfeI AGC'GCT
Cuts at: 2686 3254 2686
Size: 568 5922
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 2604 3477 5389 6366 1203
Size: 1401 873 1912 977 1327
Fragments arranged by size:
1912 1401 1327 977 873
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 5219 5219
Size: 6490
AleI CACnn'nnGTG
Cuts at: 1166 3576 1166
Size: 2410 4080
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3013 1184
Size: 1829 4661
AlwNI CAG_nnn'CTG
Cuts at: 3411 4233 5805 3411
Size: 822 1572 4096
Fragments arranged by size:
4096 1572 822
ApaI G_GGCC'C
Cuts at: 2445 3318 2445
Size: 873 5617
ApaLI G'TGCA_C
Cuts at: 3481 4547 5703 3481
Size: 1066 1156 4268
Fragments arranged by size:
4268 1156 1066
ApoI r'AATT_y
Cuts at: 1493 3799 4025 4177 6286 1493
Size: 2306 226 152 2109 1697
Fragments arranged by size:
2306 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5044 160
Size: 872 4012 1606
Fragments arranged by size:
4012 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 2438 3815 1870
Size: 73 495 1377 4545
Fragments arranged by size:
4545 1377 495 73
AvrII C'CTAG_G
Cuts at: 3068 3068
Size: 6490
BamHI G'GATC_C
Cuts at: 2805 3123 3459 3820 4187 2805
Size: 318 336 361 367 5108
Fragments arranged by size:
5108 367 361 336 318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2423 2489 2675 2816
Size: 325 217 627 333 303 66 186 141
Cuts at: 2816 2873 3074 3811 5266 618
Size: 57 201 737 1455 1842
Fragments arranged by size:
1842 1455 737 627 333 325 303 217
201 186 141 66 57
BanII G_rGCy'C
Cuts at: 1391 2445 3318 3770 3809 1391
Size: 1054 873 452 39 4072
Fragments arranged by size:
4072 1054 873 452 39
BbeI G_GCGC'C
Cuts at: 2124 2427 2493 2877 2124
Size: 303 66 384 5737
Fragments arranged by size:
5737 384 303 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5519
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3214 3248 4681 4715 1903
Size: 34 50 34 1193 34 1433 34 3678
Fragments arranged by size:
3678 1433 1193 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4382 5592 4382
Size: 1210 5280
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6490
BfrBI ATG'CAT
Cuts at: 2641 3138 2641
Size: 497 5993
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2318 2722 5101
Size: 107 122 71 631 605 646 404 2379
Cuts at: 5101 136
Size: 1525
Fragments arranged by size:
2379 1525 646 631 605 404 122 107
71
BglII A'GATC_T
Cuts at: 6485 6485
Size: 6490
BlpI GC'TnA_GC
Cuts at: 2190 3719 3907 2190
Size: 1529 188 4773
Fragments arranged by size:
4773 1529 188
Bme1580I G_kGCm'C
Cuts at: 1790 2445 2636 2678 2772 3077 3318 3485 4551
Size: 655 191 42 94 305 241 167 1066
Cuts at: 4551 5707 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1066 655 305 241 191 167
94 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6490
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3520 4221 5179
Size: 416 53 83 399 24 2103 701 958
Cuts at: 5179 442
Size: 1753
Fragments arranged by size:
2103 1753 958 701 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3681 5150 1239
Size: 2442 1469 2579
Fragments arranged by size:
2579 2442 1469
Bpu10I CC'TnA_GC
Cuts at: 2135 2135
Size: 6490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2093 4615 5480 5778 2093
Size: 2522 865 298 2805
Fragments arranged by size:
2805 2522 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2013 2135 2330 5153 882
Size: 617 514 122 195 2823 2219
Fragments arranged by size:
2823 2219 617 514 195 122
BsaAI yAC'GTr
Cuts at: 493 1204 3478 3562 4281 493
Size: 711 2274 84 719 2702
Fragments arranged by size:
2702 2274 719 711 84
BsaBI GATnn'nnATC
Cuts at: 6484 6484
Size: 6490
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 2424 2490 2874
Size: 53 83 186 936 588 303 66 384
Cuts at: 2874 3201 4297 4679 5314 275
Size: 327 1096 382 635 1451
Fragments arranged by size:
1451 1096 936 635 588 384 382 327
303 186 83 66 53
BsaWI w'CCGG_w
Cuts at: 4923 5321 5595 5742 6153 4923
Size: 398 274 147 411 5260
Fragments arranged by size:
5260 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6265
Fragments arranged by size:
6265 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2224 2266 2275 2531 3709 1186
Size: 1038 42 9 256 1178 3967
Fragments arranged by size:
3967 1178 1038 256 42 9
BseYI C'CCAG_C
Cuts at: 2053 3246 3453 3537 3714 5693 6189 2053
Size: 1193 207 84 177 1979 496 2354
Fragments arranged by size:
2354 1979 1193 496 207 177 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2636 2740 2885 3211 3301 1163
Size: 573 900 104 145 326 90 4352
Fragments arranged by size:
4352 900 573 326 145 104 90
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2304 2694 3940 4701 5729 1540
Size: 275 186 303 390 1246 761 1028 2301
Fragments arranged by size:
2301 1246 1028 761 390 303 275 186
BsiHKAI G_wGCw'C
Cuts at: 3485 4551 4636 5707 3485
Size: 1066 85 1071 4268
Fragments arranged by size:
4268 1071 1066 85
BsmI GAATG_Cn'
Cuts at: 4001 4094 4001
Size: 93 6397
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2445 2636 2678 2772 3077 3318 3485
Size: 399 655 191 42 94 305 241 167
Cuts at: 3485 3770 3809 4551 4636 5707 1391
Size: 285 39 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 655 399 305 285 241
191 167 94 85 42 39
BspEI T'CCGG_A
Cuts at: 6153 6153
Size: 6490
BspHI T'CATG_A
Cuts at: 4379 6109 4379
Size: 1730 4760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 2742 3845 844
Size: 1226 672 1103 3489
Fragments arranged by size:
3489 1226 1103 672
BsrBI CCG'CTC
Cuts at: 1394 1823 2107 2566 4377 1394
Size: 429 284 459 1811 3507
Fragments arranged by size:
3507 1811 459 429 284
BsrDI GCAATG_nn'
Cuts at: 65 1248 3138 4985 5159 65
Size: 1183 1890 1847 174 1396
Fragments arranged by size:
1890 1847 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2251 2338 2353 2664 3582 5134 5321
Size: 110 441 87 15 311 918 1552 187
Cuts at: 5321 1700
Size: 2869
Fragments arranged by size:
2869 1552 918 441 311 187 110 87
15
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6490
BssHII G'CGCG_C
Cuts at: 1925 2139 2185 1925
Size: 214 46 6230
Fragments arranged by size:
6230 214 46
BssSI C'ACGA_G
Cuts at: 4550 5562 4550
Size: 1012 5478
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2659 794
Size: 1865 4625
BstBI TT'CG_AA
Cuts at: 5294 5294
Size: 6490
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6490
BstYI r'GATC_y
Cuts at: 1972 2805 3123 3459 3820 4187 4572 4589 6041
Size: 833 318 336 361 367 385 17 1452
Cuts at: 6041 6127 6139 6485 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 833 385 367 361 346 336
318 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2124 2216 513
Size: 552 1059 92 4787
Fragments arranged by size:
4787 1059 552 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5751
Fragments arranged by size:
5751 683 56
BtsI GCAGTG_nn'
Cuts at: 2648 2995 3440 3517 4006 4800 4820 2648
Size: 347 445 77 489 794 20 4318
Fragments arranged by size:
4318 794 489 445 347 77 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2437 2472 3765 3800 529
Size: 35 1873 35 1293 35 3219
Fragments arranged by size:
3219 1873 1293 35 35 35
DraI TTT'AAA
Cuts at: 3794 3794
Size: 6490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5497 809
Size: 507 4181 1802
Fragments arranged by size:
4181 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2539 2691
Size: 54 1425 50 275 18 471 238 152
Cuts at: 2691 3514 3937 4826 6455 8
Size: 823 423 889 1629 43
Fragments arranged by size:
1629 1425 889 823 471 423 275 238
152 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2301 2691 3937 1537
Size: 275 489 390 1246 4090
Fragments arranged by size:
4090 1246 489 390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2157 2266 3303 4420 1353
Size: 734 70 109 1037 1117 3423
Fragments arranged by size:
3423 1117 1037 734 109 70
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2187 2227 2260 2269 5077 5451
Size: 1064 138 598 40 33 9 2808 374
Cuts at: 5451 5597 387
Size: 146 1280
Fragments arranged by size:
2808 1280 1064 598 374 146 138 40
33 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2138 3528 3681 4553 5150 5937
Size: 393 398 108 1390 153 872 597 787
Cuts at: 5937 6205 1239
Size: 268 1524
Fragments arranged by size:
1524 1390 872 787 597 398 393 268
153 108
EcoNI CCTnn'n_nnAGG
Cuts at: 2091 2835 2091
Size: 744 5746
EcoO109I rG'GnC_Cy
Cuts at: 2731 2731
Size: 6490
EcoRI G'AATT_C
Cuts at: 3799 4177 3799
Size: 378 6112
EcoRV GAT'ATC
Cuts at: 3064 3064
Size: 6490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2861 3393 2861
Size: 532 5958
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 2124 2427 2493 2688 2877 3256 5637 1678
Size: 446 303 66 195 189 379 2381 2531
Fragments arranged by size:
2531 2381 446 379 303 195 189 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5131 5163 5205 5237 1373
Size: 32 135 32 3559 32 42 32 2626
Fragments arranged by size:
3559 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3834 4080 669
Size: 988 281 1896 246 3079
Fragments arranged by size:
3079 1896 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6490
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KasI G'GCGC_C
Cuts at: 2120 2423 2489 2873 2120
Size: 303 66 384 5737
Fragments arranged by size:
5737 384 303 66
KpnI G_GTAC'C
Cuts at: 3815 3815
Size: 6490
MfeI C'AATT_G
Cuts at: 4089 4089
Size: 6490
MluI A'CGCG_T
Cuts at: 6366 6366
Size: 6490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5604 5788 1305
Size: 4299 184 2007
Fragments arranged by size:
4299 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2541 10
Size: 54 1425 343 709 3959
Fragments arranged by size:
3959 1425 709 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3576 4448 4807 4966 518
Size: 648 2410 872 359 159 2042
Fragments arranged by size:
2410 2042 872 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2126 2218 2306 2350 2378 2398 2505
Size: 512 8 92 88 44 28 20 107
Cuts at: 2505 3457 3718 3733 3906 4583 5731 5976 1606
Size: 952 261 15 173 677 1148 245 2120
Fragments arranged by size:
2120 1148 952 677 512 261 245 173
107 92 88 44 28 20 15 8
NaeI GCC'GGC
Cuts at: 2253 2666 2253
Size: 413 6077
NarI GG'CG_CC
Cuts at: 2121 2424 2490 2874 2121
Size: 303 66 384 5737
Fragments arranged by size:
5737 384 303 66
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5938
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6490
NgoMIV G'CCGG_C
Cuts at: 2251 2664 2251
Size: 413 6077
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 2301 2691 3937 2301
Size: 390 1246 4854
Fragments arranged by size:
4854 1246 390
NruI TCG'CGA
Cuts at: 2742 2742
Size: 6490
NsiI A_TGCA'T
Cuts at: 2643 3140 2643
Size: 497 5993
NspI r_CATG'y
Cuts at: 1132 1229 2608 3848 5393 1132
Size: 97 1379 1240 1545 2229
Fragments arranged by size:
2229 1545 1379 1240 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6490
PciI A'CATG_T
Cuts at: 2604 5389 2604
Size: 2785 3705
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6490
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PmlI CAC'GTG
Cuts at: 3478 3562 3478
Size: 84 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2403 2435 4559 4591 6072 6104 2403
Size: 32 2124 32 1481 32 2789
Fragments arranged by size:
2789 2124 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2731 2731
Size: 6490
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6490
PsiI TTA'TAA
Cuts at: 4060 4060
Size: 6490
PspOMI G'GGCC_C
Cuts at: 2441 3314 2441
Size: 873 5617
PstI C_TGCA'G
Cuts at: 830 2067 2510 3210 3842 830
Size: 1237 443 700 632 3478
Fragments arranged by size:
3478 1237 700 632 443
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
PvuII CAG'CTG
Cuts at: 2350 3718 2350
Size: 1368 5122
SacII CC_GC'GG
Cuts at: 2127 2219 2127
Size: 92 6398
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SbfI CC_TGCA'GG
Cuts at: 3842 3842
Size: 6490
ScaI AGT'ACT
Cuts at: 2764 4738 6450 2764
Size: 1974 1712 2804
Fragments arranged by size:
2804 1974 1712
SfcI C'TryA_G
Cuts at: 826 1045 2063 2506 2927 3206 3838 4973 5654
Size: 219 1018 443 421 279 632 1135 681
Cuts at: 5654 5845 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1018 681 632 443 421 279
219 191
SfoI GGC'GCC
Cuts at: 2122 2425 2491 2875 2122
Size: 303 66 384 5737
Fragments arranged by size:
5737 384 303 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 2440 3817 1872
Size: 568 1377 4545
Fragments arranged by size:
4545 1377 568
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 4594 5495 5757 820
Size: 197 926 165 2486 901 262 1553
Fragments arranged by size:
2486 1553 926 901 262 197 165
SnaBI TAC'GTA
Cuts at: 493 4281 493
Size: 3788 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6490
SphI G_CATG'C
Cuts at: 1132 3848 1132
Size: 2716 3774
SspI AAT'ATT
Cuts at: 5 52 4414 5
Size: 47 4362 2081
Fragments arranged by size:
4362 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6490
StyI C'CwwG_G
Cuts at: 513 1065 2406 2836 3068 3929 513
Size: 552 1341 430 232 861 3074
Fragments arranged by size:
3074 1341 861 552 430 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3260 3467 3728 4517 4534 4687 4872
Size: 1255 1680 207 261 789 17 153 185
Cuts at: 4872 5317 325
Size: 445 1498
Fragments arranged by size:
1680 1498 1255 789 445 261 207 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2762 3027 4736
Size: 274 80 33 51 1443 785 265 1709
Cuts at: 4736 6448 96
Size: 1712 138
Fragments arranged by size:
1712 1709 1443 785 274 265 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3173 3205 6450 6482 3173
Size: 32 3245 32 3181
Fragments arranged by size:
3245 3181 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3826 3826
Size: 6490
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6490
XmaI C'CCGG_G
Cuts at: 1870 2438 3815 1870
Size: 568 1377 4545
Fragments arranged by size:
4545 1377 568
XmnI GAAnn'nnTTC
Cuts at: 3041 4619 3041
Size: 1578 4912
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbvCI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI PacI
PfoI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SrfI SwaI XcmI