Restriction map for
pFN21AE1253
(Circular) (Six-base) MAPSORT of: pFN21AE1253.seq Check: 468 from: 1 to: 6295
pFN21AE1253 6295 bp
With 182 enzymes: SgfI *
March 28, 2012 12:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2151 3638 1937
Size: 214 1487 4594
Fragments arranged by size:
4594 1487 214
Acc65I G'GTAC_C
Cuts at: 3374 3616 3374
Size: 242 6053
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4358 5742 6010 1632
Size: 2726 1384 268 1917
Fragments arranged by size:
2726 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 2351 2922 5194 6171 1203
Size: 1148 571 2272 977 1327
Fragments arranged by size:
2272 1327 1148 977 571
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 2948 5024 2948
Size: 2076 4219
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2499 1184
Size: 1315 4980
AlwNI CAG_nnn'CTG
Cuts at: 2296 4038 5610 2296
Size: 1742 1572 2981
Fragments arranged by size:
2981 1742 1572
ApaI G_GGCC'C
Cuts at: 2410 2410
Size: 6295
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 2492 3317 3604 3830 3982 6091 1493
Size: 999 825 287 226 152 2109 1697
Fragments arranged by size:
2109 1697 999 825 287 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 2334 3257 3620 1870
Size: 73 391 923 363 4545
Fragments arranged by size:
4545 923 391 363 73
BamHI G'GATC_C
Cuts at: 3625 3992 3625
Size: 367 5928
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2785 3374 3616 5071 618
Size: 325 217 627 998 589 242 1455 1842
Fragments arranged by size:
1842 1455 998 627 589 325 242 217
BanII G_rGCy'C
Cuts at: 1391 2250 2410 3184 3228 3580 3614 1391
Size: 859 160 774 44 352 34 4072
Fragments arranged by size:
4072 859 774 352 160 44 34
BbeI G_GCGC'C
Cuts at: 2789 2789
Size: 6295
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3201 928
Size: 971 1302 4022
Fragments arranged by size:
4022 1302 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4486 4520 1903
Size: 34 2549 34 3678
Fragments arranged by size:
3678 2549 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4187 5397 4187
Size: 1210 5085
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2538 4906 136
Size: 107 122 71 631 605 866 2368 1525
Fragments arranged by size:
2368 1525 866 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2609 6290 2609
Size: 3681 2614
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 2410 4356 5512 1790
Size: 620 1946 1156 2573
Fragments arranged by size:
2573 1946 1156 620
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6295
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4026 4984 442
Size: 416 53 83 399 24 2609 958 1753
Fragments arranged by size:
2609 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2032 2032
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2626 2762 3233 4955 1239
Size: 995 392 136 471 1722 2579
Fragments arranged by size:
2579 1722 995 471 392 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3012 3212 4420 5285 5583 3012
Size: 200 1208 865 298 3724
Fragments arranged by size:
3724 1208 865 298 200
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2218 3228 4958 882
Size: 617 719 1010 1730 2219
Fragments arranged by size:
2219 1730 1010 719 617
BsaAI yAC'GTr
Cuts at: 493 1204 4086 493
Size: 711 2882 2702
Fragments arranged by size:
2882 2702 711
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2786 4102 4484 5119
Size: 53 83 186 936 1253 1316 382 635
Cuts at: 5119 275
Size: 1451
Fragments arranged by size:
1451 1316 1253 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4728 5126 5400 5547 5958 4728
Size: 398 274 147 411 5065
Fragments arranged by size:
5065 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3367 3397 1178
Size: 30 165 30 1964 30 4076
Fragments arranged by size:
4076 1964 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6295
BseYI C'CCAG_C
Cuts at: 2243 2981 5498 5994 2243
Size: 738 2517 496 2544
Fragments arranged by size:
2544 2517 738 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5722
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2343 3228 3273 4356 4441 5512 2343
Size: 885 45 1083 85 1071 3126
Fragments arranged by size:
3126 1083 1071 885 85 45
BsmI GAATG_Cn'
Cuts at: 2758 3806 3899 2758
Size: 1048 93 5154
Fragments arranged by size:
5154 1048 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2250 2343 2410 3184 3228 3273 3580
Size: 399 460 93 67 774 44 45 307
Cuts at: 3580 3614 4356 4441 5512 1391
Size: 34 742 85 1071 2174
Fragments arranged by size:
2174 1071 774 742 460 399 307 93
85 67 45 44 34
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2236 2276 2539 3650 844
Size: 1392 40 263 1111 3489
Fragments arranged by size:
3489 1392 1111 263 40
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 1394
Size: 429 2359 3507
Fragments arranged by size:
3507 2359 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4790 4964 65
Size: 1183 3542 174 1396
Fragments arranged by size:
3542 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2788 4939 5126 1700
Size: 110 978 2151 187 2869
Fragments arranged by size:
2869 2151 978 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6295
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2090 2453 794
Size: 1296 363 4636
Fragments arranged by size:
4636 1296 363
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 3064 1184
Size: 1880 4415
BstXI CCAn_nnnn'nTGG
Cuts at: 2420 2594 2420
Size: 174 6121
BstYI r'GATC_y
Cuts at: 1972 2609 3545 3625 3992 4377 4394 5846 5932
Size: 637 936 80 367 385 17 1452 86
Cuts at: 5932 5944 6290 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 936 637 385 367 346 86
80 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2635 513
Size: 552 939 631 4173
Fragments arranged by size:
4173 939 631 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2656 487
Size: 683 56 1430 4126
Fragments arranged by size:
4126 1430 683 56
BtsI GCAGTG_nn'
Cuts at: 2297 2629 3811 4605 4625 2297
Size: 332 1182 794 20 3967
Fragments arranged by size:
3967 1182 794 332 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DraIII CAC_nnn'GTG
Cuts at: 3277 3277
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5302 809
Size: 507 3986 1802
Fragments arranged by size:
3986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2075 2539 3742
Size: 54 1425 50 275 18 245 464 1203
Cuts at: 3742 4631 6260 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1203 889 464 275 245 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2840 3122 3478 4225 1353
Size: 1487 282 356 747 3423
Fragments arranged by size:
3423 1487 747 356 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3226 3226
Size: 6295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2626 2762 3233 4358 4955 5742
Size: 393 602 392 136 471 1125 597 787
Cuts at: 5742 6010 1239
Size: 268 1524
Fragments arranged by size:
1524 1125 787 602 597 471 393 392
268 136
EcoO109I rG'GnC_Cy
Cuts at: 2407 2827 2942 3253 2407
Size: 420 115 311 5449
Fragments arranged by size:
5449 420 311 115
EcoRI G'AATT_C
Cuts at: 3604 3982 3604
Size: 378 5917
EcoRV GAT'ATC
Cuts at: 2683 2683
Size: 6295
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 2789 5442 1678
Size: 1111 2653 2531
Fragments arranged by size:
2653 2531 1111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 2601 2633 2934
Size: 32 135 32 974 32 23 32 301
Cuts at: 2934 2966 3143 3175 4936 4968 5010 5042 1373
Size: 32 177 32 1761 32 42 32 2626
Fragments arranged by size:
2626 1761 974 301 177 135 42 32
32 32 32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 2152 2805 3098 3639 3885 669
Size: 988 281 214 653 293 541 246 3079
Fragments arranged by size:
3079 988 653 541 293 281 246 214
HindIII A'AGCT_T
Cuts at: 748 2204 2709 748
Size: 1456 505 4334
Fragments arranged by size:
4334 1456 505
HpaI GTT'AAC
Cuts at: 2805 3885 2805
Size: 1080 5215
KasI G'GCGC_C
Cuts at: 2785 2785
Size: 6295
KpnI G_GTAC'C
Cuts at: 3378 3620 3378
Size: 242 6053
MfeI C'AATT_G
Cuts at: 3894 3894
Size: 6295
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3243 5409 5593 1305
Size: 1938 2166 184 2007
Fragments arranged by size:
2166 2007 1938 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4473
Fragments arranged by size:
4473 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4253 4612 4771 518
Size: 648 3087 359 159 2042
Fragments arranged by size:
3087 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2104 3711 4388 5536 5781 1606
Size: 498 1607 677 1148 245 2120
Fragments arranged by size:
2120 1607 1148 677 498 245
NaeI GCC'GGC
Cuts at: 2790 2790
Size: 6295
NarI GG'CG_CC
Cuts at: 2786 2786
Size: 6295
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4804
Fragments arranged by size:
4804 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NgoMIV G'CCGG_C
Cuts at: 2788 2788
Size: 6295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 2329 2355 2377 2532 2914 2926 3653
Size: 97 1100 26 22 155 382 12 727
Cuts at: 3653 5198 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1100 727 382 155 97 26
22 12
PasI CC'CwG_GG
Cuts at: 1348 2196 1348
Size: 848 5447
PciI A'CATG_T
Cuts at: 2351 2922 5194 2351
Size: 571 2272 3452
Fragments arranged by size:
3452 2272 571
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 1108
Size: 1149 5146
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2027 2059 4364 4396 5877 5909 2027
Size: 32 2305 32 1481 32 2413
Fragments arranged by size:
2413 2305 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2827 2942 2827
Size: 115 6180
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 3865 3865
Size: 6295
PspOMI G'GGCC_C
Cuts at: 2406 2406
Size: 6295
PstI C_TGCA'G
Cuts at: 830 2292 3647 830
Size: 1462 1355 3478
Fragments arranged by size:
3478 1462 1355
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
SacI G_AGCT'C
Cuts at: 3228 3228
Size: 6295
SalI G'TCGA_C
Cuts at: 1936 2150 3637 1936
Size: 214 1487 4594
Fragments arranged by size:
4594 1487 214
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SfcI C'TryA_G
Cuts at: 826 1045 2288 3643 4778 5459 5650 826
Size: 219 1243 1355 1135 681 191 1471
Fragments arranged by size:
1471 1355 1243 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2787 2787
Size: 6295
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 2336 3622 1872
Size: 464 1286 4545
Fragments arranged by size:
4545 1286 464
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3027 3227 4399 5300 5562 820
Size: 197 926 1084 200 1172 901 262 1553
Fragments arranged by size:
1553 1172 1084 926 901 262 200 197
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 2377 2532 3653 1132
Size: 1245 155 1121 3774
Fragments arranged by size:
3774 1245 1121 155
SspI AAT'ATT
Cuts at: 5 52 2592 4219 5
Size: 47 2540 1627 2081
Fragments arranged by size:
2540 2081 1627 47
StuI AGG'CCT
Cuts at: 1517 3104 1517
Size: 1587 4708
StyI C'CwwG_G
Cuts at: 513 1065 2004 2823 3184 3734 513
Size: 552 939 819 361 550 3074
Fragments arranged by size:
3074 939 819 552 550 361
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2973 4322 4339 4492 4677 5122 325
Size: 1255 1393 1349 17 153 185 445 1498
Fragments arranged by size:
1498 1393 1349 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2381 4541 6253
Size: 274 80 33 51 1443 404 2160 1712
Cuts at: 6253 96
Size: 138
Fragments arranged by size:
2160 1712 1443 404 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6255 6287 6255
Size: 32 6263
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6295
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2591 2591
Size: 6295
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 2334 3620 1870
Size: 464 1286 4545
Fragments arranged by size:
4545 1286 464
XmnI GAAnn'nnTTC
Cuts at: 2849 4424 2849
Size: 1575 4720
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI
SwaI