Restriction map for
pFN21AE1250
(Circular) (Six-base) MAPSORT of: pFN21AE1250.seq Check: 3527 from: 1 to: 6223
pFN21AE1250 6223 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2400 3491 2400
Size: 1091 5132
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2013 4033 278
Size: 53 83 186 936 477 2020 2468
Fragments arranged by size:
2468 2020 936 477 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3566 1937
Size: 1629 4594
Acc65I G'GTAC_C
Cuts at: 3544 3544
Size: 6223
AclI AA'CG_TT
Cuts at: 1563 4350 4723 1563
Size: 2787 373 3063
Fragments arranged by size:
3063 2787 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2661 2758 2945 4286 5670 5938 1632
Size: 1029 97 187 1341 1384 268 1917
Fragments arranged by size:
1917 1384 1341 1029 268 187 97
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6026
AflIII A'CryG_T
Cuts at: 1203 2829 5122 6099 1203
Size: 1626 2293 977 1327
Fragments arranged by size:
2293 1626 1327 977
AgeI A'CCGG_T
Cuts at: 5054 5054
Size: 6223
AhdI GACnn_n'nnGTC
Cuts at: 4952 4952
Size: 6223
AleI CACnn'nnGTG
Cuts at: 1166 2508 1166
Size: 1342 4881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6223
AlwNI CAG_nnn'CTG
Cuts at: 3220 3483 3966 5538 3220
Size: 263 483 1572 3905
Fragments arranged by size:
3905 1572 483 263
ApaI G_GGCC'C
Cuts at: 2472 2472
Size: 6223
ApaLI G'TGCA_C
Cuts at: 4280 5436 4280
Size: 1156 5067
ApoI r'AATT_y
Cuts at: 1493 3532 3758 3910 6019 1493
Size: 2039 226 152 2109 1697
Fragments arranged by size:
2109 2039 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4777 160
Size: 872 3745 1606
Fragments arranged by size:
3745 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2455 2465 2659 3394 3548 1870
Size: 73 108 404 10 194 735 154 4545
Fragments arranged by size:
4545 735 404 194 154 108 73 10
BamHI G'GATC_C
Cuts at: 3398 3553 3920 3398
Size: 155 367 5701
Fragments arranged by size:
5701 367 155
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 3245 3544 4999 618
Size: 325 217 627 242 1216 299 1455 1842
Fragments arranged by size:
1842 1455 1216 627 325 299 242 217
BanII G_rGCy'C
Cuts at: 1391 2154 2472 2969 3140 3341 3542 1391
Size: 763 318 497 171 201 201 4072
Fragments arranged by size:
4072 763 497 318 201 201 171
BbeI G_GCGC'C
Cuts at: 2033 3249 2033
Size: 1216 5007
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2205 2903 928
Size: 971 306 698 4248
Fragments arranged by size:
4248 971 698 306
BbvCI CC'TCA_GC
Cuts at: 2516 2516
Size: 6223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4414 4448 1903
Size: 34 2477 34 3678
Fragments arranged by size:
3678 2477 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4115 5325 4115
Size: 1210 5013
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6223
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2063 2072 2945
Size: 107 122 71 631 605 391 9 873
Cuts at: 2945 4834 136
Size: 1889 1525
Fragments arranged by size:
1889 1525 873 631 605 391 122 107
71 9
BglII A'GATC_T
Cuts at: 2655 6218 2655
Size: 3563 2660
BlpI GC'TnA_GC
Cuts at: 3197 3640 3197
Size: 443 5780
Bme1580I G_kGCm'C
Cuts at: 1790 2472 2515 4284 5440 1790
Size: 682 43 1769 1156 2573
Fragments arranged by size:
2573 1769 1156 682 43
BmgBI CAC'GTC
Cuts at: 1318 2830 1318
Size: 1512 4711
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2805 3451 3453
Size: 416 53 83 399 24 1388 646 2
Cuts at: 3453 3954 4912 442
Size: 501 958 1753
Fragments arranged by size:
1753 1388 958 646 501 416 399 83
53 24 2
BmtI G_CTAG'C
Cuts at: 1056 2378 2952 1056
Size: 1322 574 4327
Fragments arranged by size:
4327 1322 574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2599 3302 3333 4883 1239
Size: 1360 703 31 1550 2579
Fragments arranged by size:
2579 1550 1360 703 31
Bpu10I CC'TnA_GC
Cuts at: 2516 2516
Size: 6223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4348 5213 5511 4348
Size: 865 298 5060
Fragments arranged by size:
5060 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2665 2978 4886 882
Size: 617 1166 313 1908 2219
Fragments arranged by size:
2219 1908 1166 617 313
BsaAI yAC'GTr
Cuts at: 493 1204 2125 4014 493
Size: 711 921 1889 2702
Fragments arranged by size:
2702 1889 921 711
BsaBI GATnn'nnATC
Cuts at: 2546 6217 2546
Size: 3671 2552
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2030 3074 3246
Size: 53 83 186 936 477 20 1044 172
Cuts at: 3246 4030 4412 5047 275
Size: 784 382 635 1451
Fragments arranged by size:
1451 1044 936 784 635 477 382 186
172 83 53 20
BsaWI w'CCGG_w
Cuts at: 2550 4656 5054 5328 5475 5886 2550
Size: 2106 398 274 147 411 2887
Fragments arranged by size:
2887 2106 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2593 2623 1178
Size: 30 165 30 1190 30 4778
Fragments arranged by size:
4778 1190 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2648 3242 1186
Size: 1462 594 4167
Fragments arranged by size:
4167 1462 594
BseYI C'CCAG_C
Cuts at: 2350 2476 2526 3133 3277 3285 3386 5426 5922
Size: 126 50 607 144 8 101 2040 496
Cuts at: 5922 2350
Size: 2651
Fragments arranged by size:
2651 2040 607 496 144 126 101 50
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 3194 3344 1163
Size: 573 501 957 150 4042
Fragments arranged by size:
4042 957 573 501 150
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2067 3673 4434 5462 1540
Size: 275 186 66 1606 761 1028 2301
Fragments arranged by size:
2301 1606 1028 761 275 186 66
BsiHKAI G_wGCw'C
Cuts at: 2154 2969 2996 4284 4369 5440 2154
Size: 815 27 1288 85 1071 2937
Fragments arranged by size:
2937 1288 1071 815 85 27
BsmI GAATG_Cn'
Cuts at: 3734 3827 3734
Size: 93 6130
BsmBI CGTCTCn'nnnn_
Cuts at: 2962 2962
Size: 6223
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2154 2472 2515 2969 2996 3140 3341
Size: 399 364 318 43 454 27 144 201
Cuts at: 3341 3542 4284 4369 5440 1391
Size: 201 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 364 318 201
201 144 85 43 27
BspEI T'CCGG_A
Cuts at: 2550 5886 2550
Size: 3336 2887
BspHI T'CATG_A
Cuts at: 4112 5842 4112
Size: 1730 4493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2400 3093 3491 3578 844
Size: 1556 693 398 87 3489
Fragments arranged by size:
3489 1556 693 398 87
BsrBI CCG'CTC
Cuts at: 1394 1823 3196 4110 1394
Size: 429 1373 914 3507
Fragments arranged by size:
3507 1373 914 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2496 4718 4892 65
Size: 1183 1248 2222 174 1396
Fragments arranged by size:
2222 1396 1248 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2062 2066 2460 4867 5054 1700
Size: 110 252 4 394 2407 187 2869
Fragments arranged by size:
2869 2407 394 252 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6223
BssHII G'CGCG_C
Cuts at: 1925 2043 1925
Size: 118 6105
BssSI C'ACGA_G
Cuts at: 4283 5295 4283
Size: 1012 5211
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6223
BstBI TT'CG_AA
Cuts at: 5027 5027
Size: 6223
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6223
BstXI CCAn_nnnn'nTGG
Cuts at: 2842 3310 2842
Size: 468 5755
BstYI r'GATC_y
Cuts at: 1972 2208 2655 3398 3553 3920 4305 4322 5774
Size: 236 447 743 155 367 385 17 1452
Cuts at: 5774 5860 5872 6218 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 743 447 385 367 346 236
155 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 2092 2293 2340 3309 513
Size: 552 939 30 58 201 47 969 3427
Fragments arranged by size:
3427 969 939 552 201 58 47 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2545 3347 487
Size: 683 56 1319 802 3363
Fragments arranged by size:
3363 1319 802 683 56
BtsI GCAGTG_nn'
Cuts at: 3106 3206 3224 3739 4533 4553 3106
Size: 100 18 515 794 20 4776
Fragments arranged by size:
4776 794 515 100 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6223
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6188
DraI TTT'AAA
Cuts at: 3527 3527
Size: 6223
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5230 809
Size: 507 3914 1802
Fragments arranged by size:
3914 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2179 2496
Size: 54 1425 50 275 18 234 115 317
Cuts at: 2496 3670 4559 6188 8
Size: 1174 889 1629 43
Fragments arranged by size:
1629 1425 1174 889 317 275 234 115
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2064 3670 1537
Size: 275 252 1606 4090
Fragments arranged by size:
4090 1606 275 252
EarI CTCTTCn'nnn_
Cuts at: 1353 2625 2774 4153 1353
Size: 1272 149 1379 3423
Fragments arranged by size:
3423 1379 1272 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2003 4810 5184 5330 387
Size: 1064 138 414 2807 374 146 1280
Fragments arranged by size:
2807 1280 1064 414 374 146 138
EcoICRI GAG'CTC
Cuts at: 2152 2967 2152
Size: 815 5408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2599 2661 2758 2945 3302 3333 4286
Size: 393 967 62 97 187 357 31 953
Cuts at: 4286 4883 5670 5938 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 967 953 787 597 393 357 268
187 97 62 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2867 2890 2867
Size: 23 6200
EcoO109I rG'GnC_Cy
Cuts at: 2469 2728 2929 3105 2469
Size: 259 201 176 5587
Fragments arranged by size:
5587 259 201 176
EcoRI G'AATT_C
Cuts at: 3532 3910 3532
Size: 378 5845
FseI GG_CCGG'CC
Cuts at: 2070 2464 2070
Size: 394 5829
FspI TGC'GCA
Cuts at: 4729 4729
Size: 6223
HaeII r_GCGC'y
Cuts at: 1678 2033 3249 5370 1678
Size: 355 1216 2121 2531
Fragments arranged by size:
2531 2121 1216 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2141 2173 2209 2241 2593
Size: 32 135 32 569 32 36 32 352
Cuts at: 2593 2625 4864 4896 4938 4970 1373
Size: 32 2239 32 42 32 2626
Fragments arranged by size:
2626 2239 569 352 135 42 36 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2160 3567 3813 669
Size: 988 281 222 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246 222
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6223
HpaI GTT'AAC
Cuts at: 3813 3813
Size: 6223
KasI G'GCGC_C
Cuts at: 2029 3245 2029
Size: 1216 5007
KpnI G_GTAC'C
Cuts at: 3548 3548
Size: 6223
MfeI C'AATT_G
Cuts at: 3822 3822
Size: 6223
MluI A'CGCG_T
Cuts at: 6099 6099
Size: 6223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2391 2619 3060 3444 5337 5521 1305
Size: 1086 228 441 384 1893 184 2007
Fragments arranged by size:
2007 1893 1086 441 384 228 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 10
Size: 54 1425 343 666 3735
Fragments arranged by size:
3735 1425 666 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 2508 3308 4181 4540 4699 518
Size: 648 1194 148 800 873 359 159 2042
Fragments arranged by size:
2042 1194 873 800 648 359 159 148
MspA1I CmG'CkG
Cuts at: 1606 2036 2407 3220 3328 3435 3639 4316 5464
Size: 430 371 813 108 107 204 677 1148
Cuts at: 5464 5709 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 813 677 430 371 245 204
108 107
NaeI GCC'GGC
Cuts at: 2064 2068 2462 2064
Size: 4 394 5825
Fragments arranged by size:
5825 394 4
NarI GG'CG_CC
Cuts at: 2030 3246 2030
Size: 1216 5007
NcoI C'CATG_G
Cuts at: 513 1065 2004 2340 3309 513
Size: 552 939 336 969 3427
Fragments arranged by size:
3427 969 939 552 336
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6223
NgoMIV G'CCGG_C
Cuts at: 2062 2066 2460 2062
Size: 4 394 5825
Fragments arranged by size:
5825 394 4
NheI G'CTAG_C
Cuts at: 1052 2374 2948 1052
Size: 1322 574 4327
Fragments arranged by size:
4327 1322 574
NotI GC'GGCC_GC
Cuts at: 3670 3670
Size: 6223
NspI r_CATG'y
Cuts at: 1132 1229 2374 3274 3581 5126 1132
Size: 97 1145 900 307 1545 2229
Fragments arranged by size:
2229 1545 1145 900 307 97
PasI CC'CwG_GG
Cuts at: 1348 3241 3498 1348
Size: 1893 257 4073
Fragments arranged by size:
4073 1893 257
PciI A'CATG_T
Cuts at: 5122 5122
Size: 6223
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2425 1108
Size: 1317 4906
PfoI T'CCnGG_A
Cuts at: 2235 2418 2235
Size: 183 6040
PmeI GTTT'AAAC
Cuts at: 3527 3527
Size: 6223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4292 4324 5805 5837 4292
Size: 32 1481 32 4678
Fragments arranged by size:
4678 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2728 2728
Size: 6223
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6223
PsiI TTA'TAA
Cuts at: 3793 3793
Size: 6223
PspOMI G'GGCC_C
Cuts at: 2468 2468
Size: 6223
PstI C_TGCA'G
Cuts at: 830 3090 3488 3575 830
Size: 2260 398 87 3478
Fragments arranged by size:
3478 2260 398 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6223
PvuII CAG'CTG
Cuts at: 2407 3220 3328 2407
Size: 813 108 5302
Fragments arranged by size:
5302 813 108
SacI G_AGCT'C
Cuts at: 2154 2969 2154
Size: 815 5408
SacII CC_GC'GG
Cuts at: 2037 2037
Size: 6223
SalI G'TCGA_C
Cuts at: 1936 3565 1936
Size: 1629 4594
SbfI CC_TGCA'GG
Cuts at: 3090 3575 3090
Size: 485 5738
ScaI AGT'ACT
Cuts at: 4471 6183 4471
Size: 1712 4511
SfcI C'TryA_G
Cuts at: 826 1045 3086 3484 3571 4706 5387 5578 826
Size: 219 2041 398 87 1135 681 191 1471
Fragments arranged by size:
2041 1471 1135 681 398 219 191 87
SfiI GGCCn_nnn'nGGCC
Cuts at: 2063 2063
Size: 6223
SfoI GGC'GCC
Cuts at: 2031 3247 2031
Size: 1216 5007
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6223
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6223
SmaI CCC'GGG
Cuts at: 1872 2053 2467 3550 1872
Size: 181 414 1083 4545
Fragments arranged by size:
4545 1083 414 181
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4327 5228 5490 820
Size: 197 926 2384 901 262 1553
Fragments arranged by size:
2384 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4014 493
Size: 3521 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6223
SphI G_CATG'C
Cuts at: 1132 3581 1132
Size: 2449 3774
SrfI GCCC'GGGC
Cuts at: 2467 2467
Size: 6223
SspI AAT'ATT
Cuts at: 5 52 4147 5
Size: 47 4095 2081
Fragments arranged by size:
4095 2081 47
StuI AGG'CCT
Cuts at: 1517 2885 3057 3092 3159 1517
Size: 1368 172 35 67 4581
Fragments arranged by size:
4581 1368 172 67 35
StyI C'CwwG_G
Cuts at: 513 1065 2004 2340 3309 3662 513
Size: 552 939 336 969 353 3074
Fragments arranged by size:
3074 969 939 552 353 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3043 4250 4267 4420 4605 5050 325
Size: 1255 1463 1207 17 153 185 445 1498
Fragments arranged by size:
1498 1463 1255 1207 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4469 6181 96
Size: 274 80 33 51 1443 2492 1712 138
Fragments arranged by size:
2492 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3174 3206 3348 3380 6183 6215 3174
Size: 32 142 32 2803 32 3182
Fragments arranged by size:
3182 2803 142 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3076 1316
Size: 1760 4463
XbaI T'CTAG_A
Cuts at: 3559 3559
Size: 6223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3055 3055
Size: 6223
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6223
XmaI C'CCGG_G
Cuts at: 1870 2051 2465 3548 1870
Size: 181 414 1083 4545
Fragments arranged by size:
4545 1083 414 181
XmnI GAAnn'nnTTC
Cuts at: 4352 4352
Size: 6223
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2011 4031 276
Size: 53 83 186 936 477 2020 2468
Fragments arranged by size:
2468 2020 936 477 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI
SwaI