Restriction map for
pFN21AE1240
(Circular) (Six-base) MAPSORT of: pFN21AE1240.seq Check: 479 from: 1 to: 5986
pFN21AE1240 5986 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2444 2444
Size: 5986
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2565 3329 1937
Size: 628 764 4594
Fragments arranged by size:
4594 764 628
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2370 2825 4049 5433 5701 1632
Size: 738 455 1224 1384 268 1917
Fragments arranged by size:
1917 1384 1224 738 455 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 2776 4885 5862 1203
Size: 1573 2109 977 1327
Fragments arranged by size:
2109 1573 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2072 1184
Size: 888 5098
AlwNI CAG_nnn'CTG
Cuts at: 3283 3729 5301 3283
Size: 446 1572 3968
Fragments arranged by size:
3968 1572 446
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 2574 2693 3295 3521 3673 5782 1493
Size: 1081 119 602 226 152 2109 1697
Fragments arranged by size:
2109 1697 1081 602 226 152 119
AseI AT'TA_AT
Cuts at: 160 1032 2652 4540 160
Size: 872 1620 1888 1606
Fragments arranged by size:
1888 1620 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 3311 1870
Size: 73 1368 4545
Fragments arranged by size:
4545 1368 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 3307 4762 618
Size: 325 217 627 249 1271 1455 1842
Fragments arranged by size:
1842 1455 1271 627 325 249 217
BanII G_rGCy'C
Cuts at: 1391 3305 1391
Size: 1914 4072
BbeI G_GCGC'C
Cuts at: 2040 2040
Size: 5986
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2813 928
Size: 971 914 4101
Fragments arranged by size:
4101 971 914
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4597 136
Size: 107 122 71 631 605 2925 1525
Fragments arranged by size:
2925 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 3403 3403
Size: 5986
Bme1580I G_kGCm'C
Cuts at: 1790 4047 5203 1790
Size: 2257 1156 2573
Fragments arranged by size:
2573 2257 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3717 4675 442
Size: 416 53 83 399 24 2300 958 1753
Fragments arranged by size:
2300 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3195 4646 1239
Size: 1956 1451 2579
Fragments arranged by size:
2579 1956 1451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4111 4976 5274 4111
Size: 865 298 4823
Fragments arranged by size:
4823 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4649 882
Size: 617 3150 2219
Fragments arranged by size:
3150 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 3793 4175 4810
Size: 53 83 186 936 504 1756 382 635
Cuts at: 4810 275
Size: 1451
Fragments arranged by size:
1756 1451 936 635 504 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2061 4419 4817 5091 5238 5649 2061
Size: 2358 398 274 147 411 2398
Fragments arranged by size:
2398 2358 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2456 2486 3129 3159 1178
Size: 30 165 30 1053 30 643 30 4005
Fragments arranged by size:
4005 1053 643 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3153 3264 3267 1186
Size: 1967 111 3 3905
Fragments arranged by size:
3905 1967 111 3
BseYI C'CCAG_C
Cuts at: 2687 5189 5685 2687
Size: 2502 496 2988
Fragments arranged by size:
2988 2502 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2093 2355 1163
Size: 573 357 262 4794
Fragments arranged by size:
4794 573 357 262
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4047 4132 5203 4047
Size: 85 1071 4830
Fragments arranged by size:
4830 1071 85
BsmI GAATG_Cn'
Cuts at: 3497 3590 3497
Size: 93 5893
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3305 4047 4132 5203 1391
Size: 399 1515 742 85 1071 2174
Fragments arranged by size:
2174 1515 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2061 5649 2061
Size: 3588 2398
BspHI T'CATG_A
Cuts at: 2403 2889 3875 5605 2403
Size: 486 986 1730 2784
Fragments arranged by size:
2784 1730 986 486
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2444 3341 844
Size: 1600 897 3489
Fragments arranged by size:
3489 1600 897
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2211 3054 3250 4481 4655 65
Size: 1183 963 843 196 1231 174 1396
Fragments arranged by size:
1396 1231 1183 963 843 196 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4630 4817 1700
Size: 110 2820 187 2869
Fragments arranged by size:
2869 2820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstXI CCAn_nnnn'nTGG
Cuts at: 2407 2407
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2162 3316 3683 4068 4085 5537 5623 5635
Size: 190 1154 367 385 17 1452 86 12
Cuts at: 5635 5981 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1154 385 367 346 190 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4495
Fragments arranged by size:
4495 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 3502 4296 4316 3502
Size: 794 20 5172
Fragments arranged by size:
5172 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2185 2220 529
Size: 35 1621 35 4295
Fragments arranged by size:
4295 1621 35 35
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3433 4322 5951
Size: 54 1425 50 275 18 1603 889 1629
Cuts at: 5951 8
Size: 43
Fragments arranged by size:
1629 1603 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2903 3916 1353
Size: 1550 1013 3423
Fragments arranged by size:
3423 1550 1013
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2370 2825 3195 4049 4646 5433 5701
Size: 393 738 455 370 854 597 787 268
Cuts at: 5701 1239
Size: 1524
Fragments arranged by size:
1524 854 787 738 597 455 393 370
268
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FspI TGC'GCA
Cuts at: 2030 4492 2030
Size: 2462 3524
HaeII r_GCGC'y
Cuts at: 1678 2040 5133 1678
Size: 362 3093 2531
Fragments arranged by size:
3093 2531 362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 2456 2488 3175
Size: 32 135 32 826 32 26 32 687
Cuts at: 3175 3207 4627 4659 4701 4733 1373
Size: 32 1420 32 42 32 2626
Fragments arranged by size:
2626 1420 826 687 135 42 32 32
32 32 32 32 32 26
HincII GTy'rAC
Cuts at: 669 1657 1938 2738 3330 3576 669
Size: 988 281 800 592 246 3079
Fragments arranged by size:
3079 988 800 592 281 246
HindIII A'AGCT_T
Cuts at: 748 2201 2525 748
Size: 1453 324 4209
Fragments arranged by size:
4209 1453 324
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KasI G'GCGC_C
Cuts at: 2036 2036
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 2982 3162 3585 2982
Size: 180 423 5383
Fragments arranged by size:
5383 423 180
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3084 5100 5284 1305
Size: 1779 2016 184 2007
Fragments arranged by size:
2016 2007 1779 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4164
Fragments arranged by size:
4164 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2405 2439 3098 3944 4303 4462
Size: 648 843 396 34 659 846 359 159
Cuts at: 4462 518
Size: 2042
Fragments arranged by size:
2042 846 843 659 648 396 359 159
34
MspA1I CmG'CkG
Cuts at: 1606 2034 2589 2843 2876 3402 4079 5227 5472
Size: 428 555 254 33 526 677 1148 245
Cuts at: 5472 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 555 526 428 254 245
33
NarI GG'CG_CC
Cuts at: 2037 2037
Size: 5986
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4495
Fragments arranged by size:
4495 939 552
NdeI CA'TA_TG
Cuts at: 387 2485 2512 2580 387
Size: 2098 27 68 3793
Fragments arranged by size:
3793 2098 68 27
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 2473 3344 4889 1132
Size: 97 1244 871 1545 2229
Fragments arranged by size:
2229 1545 1244 871 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2516 1108
Size: 1408 4578
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PstI C_TGCA'G
Cuts at: 830 3338 830
Size: 2508 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 2589 2843 2876 2589
Size: 254 33 5699
Fragments arranged by size:
5699 254 33
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 2961 3334 4469 5150 5341 826
Size: 219 1916 373 1135 681 191 1471
Fragments arranged by size:
1916 1471 1135 681 373 219 191
SfoI GGC'GCC
Cuts at: 2038 2038
Size: 5986
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4090 4991 5253 820
Size: 197 926 2147 901 262 1553
Fragments arranged by size:
2147 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 2620 3910 5
Size: 47 2568 1290 2081
Fragments arranged by size:
2568 2081 1290 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2004 3425 513
Size: 552 939 1421 3074
Fragments arranged by size:
3074 1421 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2170 2321 4013 4030 4183 4368 4813
Size: 1255 590 151 1692 17 153 185 445
Cuts at: 4813 325
Size: 1498
Fragments arranged by size:
1692 1498 1255 590 445 185 153 151
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2855 3082 4232
Size: 274 80 33 51 1443 878 227 1150
Cuts at: 4232 5944 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1150 878 274 227 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2825 2011
Size: 814 5172
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 2709 4115 2709
Size: 1406 4580
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI