Restriction map for
pFN21AE1232
(Circular) (Six-base) MAPSORT of: pFN21AE1232.seq Check: 3339 from: 1 to: 5800
pFN21AE1232 5800 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3863 5247 5515 1632
Size: 2231 1384 268 1917
Fragments arranged by size:
2231 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2699 820
Size: 197 1682 3921
Fragments arranged by size:
3921 1682 197
AflIII A'CryG_T
Cuts at: 1203 2058 2626 4699 5676 1203
Size: 855 568 2073 977 1327
Fragments arranged by size:
2073 1327 977 855 568
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 2449 3543 5115 2449
Size: 1094 1572 3134
Fragments arranged by size:
3134 1572 1094
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 2333 3109 3335 3487 5596 1493
Size: 840 776 226 152 2109 1697
Fragments arranged by size:
2109 1697 840 776 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 3125 1870
Size: 73 1182 4545
Fragments arranged by size:
4545 1182 73
BamHI G'GATC_C
Cuts at: 2008 3130 3497 2008
Size: 1122 367 4311
Fragments arranged by size:
4311 1122 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3121 4576 618
Size: 325 217 627 1334 1455 1842
Fragments arranged by size:
1842 1455 1334 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2084 3119 1391
Size: 693 1035 4072
Fragments arranged by size:
4072 1035 693
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2421 2432 3066 928
Size: 971 522 11 634 3662
Fragments arranged by size:
3662 971 634 522 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 2326 1548
Size: 778 5022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4411 136
Size: 107 122 71 631 605 2739 1525
Fragments arranged by size:
2739 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 2083 3217 2083
Size: 1134 4666
Bme1580I G_kGCm'C
Cuts at: 1790 3861 5017 1790
Size: 2071 1156 2573
Fragments arranged by size:
2573 2071 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3531 4489 442
Size: 416 53 83 399 24 2114 958 1753
Fragments arranged by size:
2114 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2103 2103
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4460 1239
Size: 3221 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2200 4463 882
Size: 617 701 2263 2219
Fragments arranged by size:
2263 2219 701 617
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3607 3989 4624 275
Size: 53 83 186 936 2074 382 635 1451
Fragments arranged by size:
2074 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5575
Fragments arranged by size:
5575 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5800
BseYI C'CCAG_C
Cuts at: 2531 5003 5499 2531
Size: 2472 496 2832
Fragments arranged by size:
2832 2472 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2331 1163
Size: 573 595 4632
Fragments arranged by size:
4632 595 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 3250 4011 5039 1540
Size: 275 186 18 1231 761 1028 2301
Fragments arranged by size:
2301 1231 1028 761 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2084 3861 3946 5017 2084
Size: 1777 85 1071 2867
Fragments arranged by size:
2867 1777 1071 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2084 3119 3861 3946 5017 1391
Size: 399 294 1035 742 85 1071 2174
Fragments arranged by size:
2174 1071 1035 742 399 294 85
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3155 844
Size: 2311 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2064 2877 4295 4469 65
Size: 1183 816 813 1418 174 1396
Fragments arranged by size:
1418 1396 1183 816 813 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2072 4444 4631 1700
Size: 110 204 58 2372 187 2869
Fragments arranged by size:
2869 2372 204 187 110 58
BsrGI T'GTAC_A
Cuts at: 96 2902 96
Size: 2806 2994
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 2561 2949 3860 4872 2561
Size: 388 911 1012 3489
Fragments arranged by size:
3489 1012 911 388
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 3055 3055
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2008 3130 3497 3882 3899 5351 5437 5449
Size: 36 1122 367 385 17 1452 86 12
Cuts at: 5449 5795 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1122 385 367 346 86 36
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2019 513
Size: 552 939 15 4294
Fragments arranged by size:
4294 939 552 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2035 487
Size: 683 56 809 4252
Fragments arranged by size:
4252 809 683 56
BtsI GCAGTG_nn'
Cuts at: 3316 4110 4130 3316
Size: 794 20 4986
Fragments arranged by size:
4986 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 2683 3104 2683
Size: 421 5379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 3247 4136
Size: 54 1425 50 275 18 186 1231 889
Cuts at: 4136 5765 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1231 889 275 186 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2016 3247 1537
Size: 275 204 1231 4090
Fragments arranged by size:
4090 1231 275 204
EarI CTCTTCn'nnn_
Cuts at: 1353 3730 1353
Size: 2377 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2001 4387 4761 4907 387
Size: 1064 138 412 2386 374 146 1280
Fragments arranged by size:
2386 1280 1064 412 374 146 138
EcoICRI GAG'CTC
Cuts at: 2082 2082
Size: 5800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3863 4460 5247 5515 1239
Size: 393 2231 597 787 268 1524
Fragments arranged by size:
2231 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2248 2248
Size: 5800
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2446 2714 2847 2446
Size: 268 133 5399
Fragments arranged by size:
5399 268 133
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 2151 4947 1678
Size: 473 2796 2531
Fragments arranged by size:
2796 2531 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 4441 4473 4515
Size: 32 135 32 449 32 2388 32 42
Cuts at: 4515 4547 1373
Size: 32 2626
Fragments arranged by size:
2626 2388 449 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2171 748
Size: 1423 4377
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 2058 5676 2058
Size: 3618 2182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2114 4914 5098 1305
Size: 809 2800 184 2007
Fragments arranged by size:
2800 2007 809 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2475 2979 3758 4117 4276 518
Size: 648 1309 504 779 359 159 2042
Fragments arranged by size:
2042 1309 779 648 504 359 159
MspA1I CmG'CkG
Cuts at: 1606 2449 3216 3893 5041 5286 1606
Size: 843 767 677 1148 245 2120
Fragments arranged by size:
2120 1148 843 767 677 245
NaeI GCC'GGC
Cuts at: 2016 2074 2016
Size: 58 5742
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NgoMIV G'CCGG_C
Cuts at: 2014 2072 2014
Size: 58 5742
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 2552 2630 2849 2897 3158 4703 1132
Size: 97 1323 78 219 48 261 1545 2229
Fragments arranged by size:
2229 1545 1323 261 219 97 78 48
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 2626 4699 2626
Size: 2073 3727
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5800
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PstI C_TGCA'G
Cuts at: 830 2147 3152 830
Size: 1317 1005 3478
Fragments arranged by size:
3478 1317 1005
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
PvuII CAG'CTG
Cuts at: 2449 2449
Size: 5800
SacI G_AGCT'C
Cuts at: 2084 2084
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 3087 4048 5760 3087
Size: 961 1712 3127
Fragments arranged by size:
3127 1712 961
SfcI C'TryA_G
Cuts at: 826 1045 2143 3148 4283 4964 5155 826
Size: 219 1098 1005 1135 681 191 1471
Fragments arranged by size:
1471 1135 1098 1005 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2699 3904 4805 5067 820
Size: 197 926 756 1205 901 262 1553
Fragments arranged by size:
1553 1205 926 901 756 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 2897 3158 1132
Size: 1765 261 3774
Fragments arranged by size:
3774 1765 261
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2243 1517
Size: 726 5074
StyI C'CwwG_G
Cuts at: 513 1065 2004 2553 3094 3239 513
Size: 552 939 549 541 145 3074
Fragments arranged by size:
3074 939 552 549 541 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2545 3061 3827 3844 3997 4182 4627
Size: 1255 965 516 766 17 153 185 445
Cuts at: 4627 325
Size: 1498
Fragments arranged by size:
1498 1255 965 766 516 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2902 3085 4046
Size: 274 80 33 51 1443 925 183 961
Cuts at: 4046 5758 96
Size: 1712 138
Fragments arranged by size:
1712 1443 961 925 274 183 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5760 5792 5760
Size: 32 5768
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2507 2507
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI