Restriction map for
pFN21AE1230
(Circular) (Six-base) MAPSORT of: pFN21AE1230.seq Check: 9454 from: 1 to: 5734
pFN21AE1230 5734 bp
With 182 enzymes: SgfI *
April 28, 2014 11:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2551 2824 2551
Size: 273 5461
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 3055 3055
Size: 5734
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2308 3797 5181 5449 1632
Size: 676 1489 1384 268 1917
Fragments arranged by size:
1917 1489 1384 676 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 4633 5610 1203
Size: 3430 977 1327
Fragments arranged by size:
3430 1327 977
AgeI A'CCGG_T
Cuts at: 2128 4565 2128
Size: 2437 3297
AhdI GACnn_n'nnGTC
Cuts at: 4463 4463
Size: 5734
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5734
AlwNI CAG_nnn'CTG
Cuts at: 2683 3477 5049 2683
Size: 794 1572 3368
Fragments arranged by size:
3368 1572 794
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 3043 3269 3421 5530 1493
Size: 1550 226 152 2109 1697
Fragments arranged by size:
2109 1697 1550 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 2425 3059 1870
Size: 73 482 634 4545
Fragments arranged by size:
4545 634 482 73
BamHI G'GATC_C
Cuts at: 2367 3064 3431 2367
Size: 697 367 4670
Fragments arranged by size:
4670 697 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2582 3055 4510 618
Size: 325 217 627 795 473 1455 1842
Fragments arranged by size:
1842 1455 795 627 473 325 217
BanII G_rGCy'C
Cuts at: 1391 2701 2813 3053 1391
Size: 1310 112 240 4072
Fragments arranged by size:
4072 1310 240 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3925 3959 1903
Size: 34 50 34 1904 34 3678
Fragments arranged by size:
3678 1904 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3626 4836 3626
Size: 1210 4524
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2580 4345 136
Size: 107 122 71 631 605 908 1765 1525
Fragments arranged by size:
1765 1525 908 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5729 5729
Size: 5734
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 2585 3795 4951 1790
Size: 795 1210 1156 2573
Fragments arranged by size:
2573 1210 1156 795
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5734
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2177 3465 4423
Size: 416 53 83 399 24 760 1288 958
Cuts at: 4423 442
Size: 1753
Fragments arranged by size:
1753 1288 958 760 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2102 2150 1056
Size: 1046 48 4640
Fragments arranged by size:
4640 1046 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2483 4394 1239
Size: 1244 1911 2579
Fragments arranged by size:
2579 1911 1244
Bpu10I CC'TnA_GC
Cuts at: 2183 2183
Size: 5734
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3011 3859 4724 5022 3011
Size: 848 865 298 3723
Fragments arranged by size:
3723 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4397 882
Size: 617 2898 2219
Fragments arranged by size:
2898 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2524 3525 493
Size: 711 1320 1001 2702
Fragments arranged by size:
2702 1320 1001 711
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3541 3923 4558 275
Size: 53 83 186 936 2008 382 635 1451
Fragments arranged by size:
2008 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2128 4167 4565 4839 4986 5397 2128
Size: 2039 398 274 147 411 2465
Fragments arranged by size:
2465 2039 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2444 2474 1178
Size: 30 165 30 1041 30 4438
Fragments arranged by size:
4438 1041 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2546 1186
Size: 1234 126 4374
Fragments arranged by size:
4374 1234 126
BseYI C'CCAG_C
Cuts at: 2174 4937 5433 2174
Size: 2763 496 2475
Fragments arranged by size:
2763 2475 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2330 2498 1163
Size: 573 417 177 168 4399
Fragments arranged by size:
4399 573 417 177 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2579 2662 3184 3945 4973 1540
Size: 275 186 578 83 522 761 1028 2301
Fragments arranged by size:
2301 1028 761 578 522 275 186 83
BsiHKAI G_wGCw'C
Cuts at: 2701 3795 3880 4951 2701
Size: 1094 85 1071 3484
Fragments arranged by size:
3484 1094 1071 85
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2585 2701 2813 3053 3795 3880 4951
Size: 399 795 116 112 240 742 85 1071
Cuts at: 4951 1391
Size: 2174
Fragments arranged by size:
2174 1071 795 742 399 240 116 112
85
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 2859 3623 5353 2859
Size: 764 1730 3240
Fragments arranged by size:
3240 1730 764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2551 2824 3089 844
Size: 1707 273 265 3489
Fragments arranged by size:
3489 1707 273 265
BsrBI CCG'CTC
Cuts at: 1394 1823 3621 1394
Size: 429 1798 3507
Fragments arranged by size:
3507 1798 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4229 4403 65
Size: 1183 2981 174 1396
Fragments arranged by size:
2981 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2128 2338 2447 2574 2661 4378 4565
Size: 110 318 210 109 127 87 1717 187
Cuts at: 4565 1700
Size: 2869
Fragments arranged by size:
2869 1717 318 210 187 127 110 109
87
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5734
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 2725 1184
Size: 1541 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 2640 2920 2640
Size: 280 5454
BstYI r'GATC_y
Cuts at: 1972 2367 2493 2880 3064 3431 3816 3833 5285
Size: 395 126 387 184 367 385 17 1452
Cuts at: 5285 5371 5383 5729 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 395 387 385 367 346 184
126 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 2355 2773 513
Size: 552 972 318 418 3474
Fragments arranged by size:
3474 972 552 418 318
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2811 487
Size: 683 56 1585 3410
Fragments arranged by size:
3410 1585 683 56
BtsI GCAGTG_nn'
Cuts at: 3250 4044 4064 3250
Size: 794 20 4920
Fragments arranged by size:
4920 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2099 2134 529
Size: 35 1535 35 4129
Fragments arranged by size:
4129 1535 35 35
DraI TTT'AAA
Cuts at: 3038 3038
Size: 5734
DraIII CAC_nnn'GTG
Cuts at: 2844 2844
Size: 5734
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2358 2576
Size: 54 1425 50 275 18 429 99 218
Cuts at: 2576 2655 2922 3181 4070 5699 8
Size: 79 267 259 889 1629 43
Fragments arranged by size:
1629 1425 889 429 275 267 259 218
99 79 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2576 3181 1537
Size: 275 764 605 4090
Fragments arranged by size:
4090 764 605 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3664 1353
Size: 2311 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2474 2919 4321 4695 4841 387
Size: 1064 138 885 445 1402 374 146 1280
Fragments arranged by size:
1402 1280 1064 885 445 374 146 138
EcoICRI GAG'CTC
Cuts at: 2699 2699
Size: 5734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2308 2483 3797 4394 5181 5449 1239
Size: 393 676 175 1314 597 787 268 1524
Fragments arranged by size:
1524 1314 787 676 597 393 268 175
EcoNI CCTnn'n_nnAGG
Cuts at: 2486 2675 2486
Size: 189 5545
EcoO109I rG'GnC_Cy
Cuts at: 2171 2554 2680 2171
Size: 383 126 5225
Fragments arranged by size:
5225 383 126
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 2355 4881 1678
Size: 677 2526 2531
Fragments arranged by size:
2531 2526 677
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2898 2930 4375 4407 4449
Size: 32 135 32 1326 32 1445 32 42
Cuts at: 4449 4481 1373
Size: 32 2626
Fragments arranged by size:
2626 1445 1326 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5734
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 3059 3059
Size: 5734
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4848 5032 1305
Size: 3543 184 2007
Fragments arranged by size:
3543 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 2924 10
Size: 54 1425 343 429 663 2820
Fragments arranged by size:
2820 1425 663 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2720 2778 2858 3692 4051 4210 518
Size: 648 1554 58 80 834 359 159 2042
Fragments arranged by size:
2042 1554 834 648 359 159 80 58
MspA1I CmG'CkG
Cuts at: 1606 2095 3150 3827 4975 5220 1606
Size: 489 1055 677 1148 245 2120
Fragments arranged by size:
2120 1148 1055 677 489 245
NaeI GCC'GGC
Cuts at: 2576 2576
Size: 5734
NcoI C'CATG_G
Cuts at: 513 1065 2037 513
Size: 552 972 4210
Fragments arranged by size:
4210 972 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NgoMIV G'CCGG_C
Cuts at: 2574 2574
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 2098 2146 1052
Size: 1046 48 4640
Fragments arranged by size:
4640 1046 48
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 3092 4637 1132
Size: 97 1863 1545 2229
Fragments arranged by size:
2229 1863 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5734
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5734
PfoI T'CCnGG_A
Cuts at: 2462 2499 2462
Size: 37 5697
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PmlI CAC'GTG
Cuts at: 2524 2524
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 5316 5348 3803
Size: 32 1481 32 4189
Fragments arranged by size:
4189 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2171 2171
Size: 5734
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5734
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PstI C_TGCA'G
Cuts at: 830 2562 3086 830
Size: 1732 524 3478
Fragments arranged by size:
3478 1732 524
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
SacI G_AGCT'C
Cuts at: 2701 2701
Size: 5734
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SanDI GG'GwC_CC
Cuts at: 2171 2171
Size: 5734
SbfI CC_TGCA'GG
Cuts at: 2562 3086 2562
Size: 524 5210
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SfcI C'TryA_G
Cuts at: 826 1045 2558 2672 2704 3082 4217 4898 5089
Size: 219 1513 114 32 378 1135 681 191
Cuts at: 5089 826
Size: 1471
Fragments arranged by size:
1513 1471 1135 681 378 219 191 114
32
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 2427 3061 1872
Size: 555 634 4545
Fragments arranged by size:
4545 634 555
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3026 3838 4739 5001 820
Size: 197 926 1083 812 901 262 1553
Fragments arranged by size:
1553 1083 926 901 812 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5734
StyI C'CwwG_G
Cuts at: 513 1065 2037 3173 513
Size: 552 972 1136 3074
Fragments arranged by size:
3074 1136 972 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3761 3778 3931 4116 4561 325
Size: 1255 2181 17 153 185 445 1498
Fragments arranged by size:
2181 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3980 5692 96
Size: 274 80 33 51 1443 2003 1712 138
Fragments arranged by size:
2003 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5694 5726 5694
Size: 32 5702
Tth111I GACn'n_nGTC
Cuts at: 1316 2618 1316
Size: 1302 4432
XbaI T'CTAG_A
Cuts at: 3070 3070
Size: 5734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2737 2737
Size: 5734
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 2425 3059 1870
Size: 555 634 4545
Fragments arranged by size:
4545 634 555
XmnI GAAnn'nnTTC
Cuts at: 3863 3863
Size: 5734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PspOMI
PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI