Restriction map for
pFN21AE1228
(Circular) (Six-base) MAPSORT of: pFN21AE1228.seq Check: 1624 from: 1 to: 5656
pFN21AE1228 5656 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3466 278
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2999 1937
Size: 1062 4594
Acc65I G'GTAC_C
Cuts at: 2173 2977 2173
Size: 804 4852
AclI AA'CG_TT
Cuts at: 1563 3783 4156 1563
Size: 2220 373 3063
Fragments arranged by size:
3063 2220 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3719 5103 5371 1632
Size: 2087 1384 268 1917
Fragments arranged by size:
2087 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2090 820
Size: 197 1073 4386
Fragments arranged by size:
4386 1073 197
AflIII A'CryG_T
Cuts at: 1203 4555 5532 1203
Size: 3352 977 1327
Fragments arranged by size:
3352 1327 977
AgeI A'CCGG_T
Cuts at: 4487 4487
Size: 5656
AhdI GACnn_n'nnGTC
Cuts at: 4385 4385
Size: 5656
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5656
AlwNI CAG_nnn'CTG
Cuts at: 2585 3399 4971 2585
Size: 814 1572 3270
Fragments arranged by size:
3270 1572 814
ApaLI G'TGCA_C
Cuts at: 2309 3713 4869 2309
Size: 1404 1156 3096
Fragments arranged by size:
3096 1404 1156
ApoI r'AATT_y
Cuts at: 1493 2396 2515 2523 2765 2965 3191 3343 5452
Size: 903 119 8 242 200 226 152 2109
Cuts at: 5452 1493
Size: 1697
Fragments arranged by size:
2109 1697 903 242 226 200 152 119
8
AseI AT'TA_AT
Cuts at: 160 1032 4210 160
Size: 872 3178 1606
Fragments arranged by size:
3178 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 1870
Size: 73 1038 4545
Fragments arranged by size:
4545 1038 73
BamHI G'GATC_C
Cuts at: 2986 3353 2986
Size: 367 5289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2173 2977 4432 618
Size: 325 217 627 386 804 1455 1842
Fragments arranged by size:
1842 1455 804 627 386 325 217
BanII G_rGCy'C
Cuts at: 1391 2975 1391
Size: 1584 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2040 2345 2702 928
Size: 971 141 305 357 3882
Fragments arranged by size:
3882 971 357 305 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2326 2360 3847 3881 1903
Size: 34 389 34 1487 34 3678
Fragments arranged by size:
3678 1487 389 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2126 2321 3548 4758 2126
Size: 195 1227 1210 3024
Fragments arranged by size:
3024 1227 1210 195
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5656
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2817 4267 136
Size: 107 122 71 631 605 1145 1450 1525
Fragments arranged by size:
1525 1450 1145 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2617 5651 2617
Size: 3034 2622
BlpI GC'TnA_GC
Cuts at: 3073 3073
Size: 5656
Bme1580I G_kGCm'C
Cuts at: 1790 2313 3717 4873 1790
Size: 523 1404 1156 2573
Fragments arranged by size:
2573 1404 1156 523
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5656
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3387 4345 442
Size: 416 53 83 399 24 1970 958 1753
Fragments arranged by size:
1970 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2004 2549 4316 1239
Size: 765 545 1767 2579
Fragments arranged by size:
2579 1767 765 545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2664 3781 4646 4944 2664
Size: 1117 865 298 3376
Fragments arranged by size:
3376 1117 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4319 882
Size: 617 2820 2219
Fragments arranged by size:
2820 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3447 493
Size: 711 2243 2702
Fragments arranged by size:
2702 2243 711
BsaBI GATnn'nnATC
Cuts at: 5650 5650
Size: 5656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3463 3845 4480 275
Size: 53 83 186 936 1930 382 635 1451
Fragments arranged by size:
1930 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4089 4487 4761 4908 5319 4089
Size: 398 274 147 411 4426
Fragments arranged by size:
4426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2880 2910 1178
Size: 30 165 30 1477 30 3924
Fragments arranged by size:
3924 1477 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2618 2621 1186
Size: 1432 3 4221
Fragments arranged by size:
4221 1432 3
BseYI C'CCAG_C
Cuts at: 2476 2944 4859 5355 2476
Size: 468 1915 496 2777
Fragments arranged by size:
2777 1915 496 468
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5083
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3106 3867 4895 1540
Size: 275 186 1105 761 1028 2301
Fragments arranged by size:
2301 1105 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2202 2313 3717 3802 4873 2202
Size: 111 1404 85 1071 2985
Fragments arranged by size:
2985 1404 1071 111 85
BsmI GAATG_Cn'
Cuts at: 3167 3260 3167
Size: 93 5563
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2313 2975 3717 3802 4873 1391
Size: 399 412 111 662 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 662 412 399 111 85
BspEI T'CCGG_A
Cuts at: 5319 5319
Size: 5656
BspHI T'CATG_A
Cuts at: 3545 5275 3545
Size: 1730 3926
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3011 844
Size: 2167 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3543 1394
Size: 429 1720 3507
Fragments arranged by size:
3507 1720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2221 4151 4325 65
Size: 1183 973 1930 174 1396
Fragments arranged by size:
1930 1396 1183 973 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4300 4487 1700
Size: 110 2490 187 2869
Fragments arranged by size:
2869 2490 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5656
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5656
BssSI C'ACGA_G
Cuts at: 3716 4728 3716
Size: 1012 4644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5656
BstBI TT'CG_AA
Cuts at: 4460 4460
Size: 5656
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5656
BstXI CCAn_nnnn'nTGG
Cuts at: 2527 2527
Size: 5656
BstYI r'GATC_y
Cuts at: 1972 2617 2986 3353 3738 3755 5207 5293 5305
Size: 645 369 367 385 17 1452 86 12
Cuts at: 5305 5651 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 645 385 369 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4165
Fragments arranged by size:
4165 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4917
Fragments arranged by size:
4917 683 56
BtsI GCAGTG_nn'
Cuts at: 3172 3966 3986 3172
Size: 794 20 4842
Fragments arranged by size:
4842 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5656
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5621
DraI TTT'AAA
Cuts at: 2065 2320 2960 2065
Size: 255 640 4761
Fragments arranged by size:
4761 640 255
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4663 809
Size: 507 3347 1802
Fragments arranged by size:
3347 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2165 3103 3992
Size: 54 1425 50 275 18 335 938 889
Cuts at: 3992 5621 8
Size: 1629 43
Fragments arranged by size:
1629 1425 938 889 335 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3103 1537
Size: 275 1291 4090
Fragments arranged by size:
4090 1291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 3586 1353
Size: 1053 1180 3423
Fragments arranged by size:
3423 1180 1053
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4243 4617 4763 387
Size: 1064 138 2654 374 146 1280
Fragments arranged by size:
2654 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2004 2549 3719 4316 5103 5371 1239
Size: 393 372 545 1170 597 787 268 1524
Fragments arranged by size:
1524 1170 787 597 545 393 372 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2853 2853
Size: 5656
EcoO109I rG'GnC_Cy
Cuts at: 2473 2473
Size: 5656
EcoRI G'AATT_C
Cuts at: 2965 3343 2965
Size: 378 5278
FspI TGC'GCA
Cuts at: 4162 4162
Size: 5656
HaeII r_GCGC'y
Cuts at: 1678 4803 1678
Size: 3125 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2880 2883 2912 2915 4297
Size: 32 135 32 1308 3 29 3 1382
Cuts at: 4297 4329 4371 4403 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1382 1308 135 42 32 32 32
32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3000 3246 669
Size: 988 281 1062 246 3079
Fragments arranged by size:
3079 1062 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2058 748
Size: 1310 4346
HpaI GTT'AAC
Cuts at: 3246 3246
Size: 5656
KpnI G_GTAC'C
Cuts at: 2177 2981 2177
Size: 804 4852
MfeI C'AATT_G
Cuts at: 3255 3255
Size: 5656
MluI A'CGCG_T
Cuts at: 5532 5532
Size: 5656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4770 4954 1305
Size: 3465 184 2007
Fragments arranged by size:
3465 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3834
Fragments arranged by size:
3834 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3614 3973 4132 518
Size: 648 2448 359 159 2042
Fragments arranged by size:
2448 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3072 3749 4897 5142 1606
Size: 1466 677 1148 245 2120
Fragments arranged by size:
2120 1466 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4165
Fragments arranged by size:
4165 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5656
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5656
NotI GC'GGCC_GC
Cuts at: 3103 3103
Size: 5656
NspI r_CATG'y
Cuts at: 1132 1229 2944 3014 4559 1132
Size: 97 1715 70 1545 2229
Fragments arranged by size:
2229 1715 1545 97 70
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5656
PciI A'CATG_T
Cuts at: 4555 4555
Size: 5656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2556 1108
Size: 1448 4208
PmeI GTTT'AAAC
Cuts at: 2960 2960
Size: 5656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 5238 5270 3725
Size: 32 1481 32 4111
Fragments arranged by size:
4111 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2473 2473
Size: 5656
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5656
PsiI TTA'TAA
Cuts at: 3226 3226
Size: 5656
PstI C_TGCA'G
Cuts at: 830 3008 830
Size: 2178 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5656
SalI G'TCGA_C
Cuts at: 1936 2998 1936
Size: 1062 4594
SanDI GG'GwC_CC
Cuts at: 2473 2473
Size: 5656
SbfI CC_TGCA'GG
Cuts at: 3008 3008
Size: 5656
ScaI AGT'ACT
Cuts at: 3904 5616 3904
Size: 1712 3944
SfcI C'TryA_G
Cuts at: 826 1045 2209 3004 4139 4820 5011 826
Size: 219 1164 795 1135 681 191 1471
Fragments arranged by size:
1471 1164 1135 795 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5656
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5656
SmaI CCC'GGG
Cuts at: 1872 2983 1872
Size: 1111 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 2679 3760 4661 4923 820
Size: 197 926 147 589 1081 901 262 1553
Fragments arranged by size:
1553 1081 926 901 589 262 197 147
SnaBI TAC'GTA
Cuts at: 493 3447 493
Size: 2954 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5656
SphI G_CATG'C
Cuts at: 1132 2944 3014 1132
Size: 1812 70 3774
Fragments arranged by size:
3774 1812 70
SspI AAT'ATT
Cuts at: 5 52 2271 3580 5
Size: 47 2219 1309 2081
Fragments arranged by size:
2219 2081 1309 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5656
StyI C'CwwG_G
Cuts at: 513 1065 2004 2884 3095 513
Size: 552 939 880 211 3074
Fragments arranged by size:
3074 939 880 552 211
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2659 2920 3683 3700 3853 4038 4483
Size: 1255 1079 261 763 17 153 185 445
Cuts at: 4483 325
Size: 1498
Fragments arranged by size:
1498 1255 1079 763 445 261 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3902 5614 96
Size: 274 80 33 51 1443 1925 1712 138
Fragments arranged by size:
1925 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5616 5648 5616
Size: 32 5624
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5656
XbaI T'CTAG_A
Cuts at: 2992 2992
Size: 5656
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5656
XmaI C'CCGG_G
Cuts at: 1870 2981 1870
Size: 1111 4545
XmnI GAAnn'nnTTC
Cuts at: 2619 2730 3785 2619
Size: 111 1055 4490
Fragments arranged by size:
4490 1055 111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3464 276
Size: 53 83 186 936 1930 2468
Fragments arranged by size:
2468 1930 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SfiI SfoI SrfI SwaI XcmI