Restriction map for
pFN21AE1227
(Circular) (Six-base) MAPSORT of: pFN21AE1227.seq Check: 8904 from: 1 to: 5644
pFN21AE1227 5644 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2486 2486
Size: 5644
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3454 278
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2987 1937
Size: 1050 4594
Acc65I G'GTAC_C
Cuts at: 2965 2965
Size: 5644
AclI AA'CG_TT
Cuts at: 1563 3771 4144 1563
Size: 2208 373 3063
Fragments arranged by size:
3063 2208 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3707 5091 5359 1632
Size: 2075 1384 268 1917
Fragments arranged by size:
2075 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5447
AflIII A'CryG_T
Cuts at: 1203 4543 5520 1203
Size: 3340 977 1327
Fragments arranged by size:
3340 1327 977
AgeI A'CCGG_T
Cuts at: 4475 4475
Size: 5644
AhdI GACnn_n'nnGTC
Cuts at: 4373 4373
Size: 5644
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5644
AlwNI CAG_nnn'CTG
Cuts at: 3387 4959 3387
Size: 1572 4072
ApaLI G'TGCA_C
Cuts at: 3701 4857 3701
Size: 1156 4488
ApoI r'AATT_y
Cuts at: 1493 2606 2953 3179 3331 5440 1493
Size: 1113 347 226 152 2109 1697
Fragments arranged by size:
2109 1697 1113 347 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4198 160
Size: 872 3166 1606
Fragments arranged by size:
3166 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
AvaI C'yCGr_G
Cuts at: 1870 1943 2116 2969 1870
Size: 73 173 853 4545
Fragments arranged by size:
4545 853 173 73
BamHI G'GATC_C
Cuts at: 2974 3341 2974
Size: 367 5277
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2965 4420 618
Size: 325 217 627 1178 1455 1842
Fragments arranged by size:
1842 1455 1178 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2963 1391
Size: 1572 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2561 2595 3835 3869 1903
Size: 34 624 34 1240 34 3678
Fragments arranged by size:
3678 1240 624 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3536 4746 3536
Size: 1210 4434
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5644
BfrBI ATG'CAT
Cuts at: 2440 2672 2440
Size: 232 5412
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2100 2160 4255
Size: 107 122 71 631 605 428 60 2095
Cuts at: 4255 136
Size: 1525
Fragments arranged by size:
2095 1525 631 605 428 122 107 71
60
BglII A'GATC_T
Cuts at: 5639 5639
Size: 5644
BlpI GC'TnA_GC
Cuts at: 3061 3061
Size: 5644
Bme1580I G_kGCm'C
Cuts at: 1790 3705 4861 1790
Size: 1915 1156 2573
Fragments arranged by size:
2573 1915 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5644
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4333 442
Size: 416 53 83 399 24 1958 958 1753
Fragments arranged by size:
1958 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5644
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2733 2753 4304 1239
Size: 1494 20 1551 2579
Fragments arranged by size:
2579 1551 1494 20
Bpu10I CC'TnA_GC
Cuts at: 2046 2046
Size: 5644
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3769 4634 4932 3769
Size: 865 298 4481
Fragments arranged by size:
4481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2385 2421 4307 882
Size: 617 886 36 1886 2219
Fragments arranged by size:
2219 1886 886 617 36
BsaAI yAC'GTr
Cuts at: 493 1204 3435 493
Size: 711 2231 2702
Fragments arranged by size:
2702 2231 711
BsaBI GATnn'nnATC
Cuts at: 5638 5638
Size: 5644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 3833 4468 275
Size: 53 83 186 936 1918 382 635 1451
Fragments arranged by size:
1918 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2412 4077 4475 4749 4896 5307 2412
Size: 1665 398 274 147 411 2749
Fragments arranged by size:
2749 1665 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 1178
Size: 30 165 30 714 30 4675
Fragments arranged by size:
4675 714 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5644
BseYI C'CCAG_C
Cuts at: 4847 5343 4847
Size: 496 5148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5071
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3094 3855 4883 1540
Size: 275 186 1093 761 1028 2301
Fragments arranged by size:
2301 1093 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 3705 3790 4861 2145
Size: 1560 85 1071 2928
Fragments arranged by size:
2928 1560 1071 85
BsmI GAATG_Cn'
Cuts at: 2672 3155 3248 2672
Size: 483 93 5068
Fragments arranged by size:
5068 483 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2099 2099
Size: 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2963 3705 3790 4861 1391
Size: 399 355 818 742 85 1071 2174
Fragments arranged by size:
2174 1071 818 742 399 355 85
BspEI T'CCGG_A
Cuts at: 5307 5307
Size: 5644
BspHI T'CATG_A
Cuts at: 2886 3533 5263 2886
Size: 647 1730 3267
Fragments arranged by size:
3267 1730 647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2486 2999 844
Size: 1642 513 3489
Fragments arranged by size:
3489 1642 513
BsrBI CCG'CTC
Cuts at: 1394 1823 2814 3531 1394
Size: 429 991 717 3507
Fragments arranged by size:
3507 991 717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2620 4139 4313 65
Size: 1183 1372 1519 174 1396
Fragments arranged by size:
1519 1396 1372 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4288 4475 1700
Size: 110 2478 187 2869
Fragments arranged by size:
2869 2478 187 110
BsrGI T'GTAC_A
Cuts at: 96 2181 96
Size: 2085 3559
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5644
BssSI C'ACGA_G
Cuts at: 3704 4716 3704
Size: 1012 4632
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5644
BstBI TT'CG_AA
Cuts at: 4448 4448
Size: 5644
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5644
BstYI r'GATC_y
Cuts at: 1972 2407 2790 2974 3341 3726 3743 5195 5281
Size: 435 383 184 367 385 17 1452 86
Cuts at: 5281 5293 5639 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 435 385 383 367 346 184
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5092
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4905
Fragments arranged by size:
4905 683 56
BtsI GCAGTG_nn'
Cuts at: 3160 3954 3974 3160
Size: 794 20 4830
Fragments arranged by size:
4830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5609
DraI TTT'AAA
Cuts at: 2948 2948
Size: 5644
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4651 809
Size: 507 3335 1802
Fragments arranged by size:
3335 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3091 3980 5609
Size: 54 1425 50 275 18 1261 889 1629
Cuts at: 5609 8
Size: 43
Fragments arranged by size:
1629 1425 1261 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3091 1537
Size: 275 1279 4090
Fragments arranged by size:
4090 1279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3574 1353
Size: 2221 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4231 4605 4751 387
Size: 1064 138 2642 374 146 1280
Fragments arranged by size:
2642 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2733 2753 3707 4304 5091 5359 1239
Size: 393 1101 20 954 597 787 268 1524
Fragments arranged by size:
1524 1101 954 787 597 393 268 20
EcoO109I rG'GnC_Cy
Cuts at: 2431 2431
Size: 5644
EcoRI G'AATT_C
Cuts at: 2953 3331 2953
Size: 378 5266
FspI TGC'GCA
Cuts at: 4150 4150
Size: 5644
HaeII r_GCGC'y
Cuts at: 1678 4791 1678
Size: 3113 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2399 2431 4285
Size: 32 135 32 545 32 250 32 1854
Cuts at: 4285 4317 4359 4391 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1854 545 250 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2503 2988 3234 669
Size: 988 281 565 485 246 3079
Fragments arranged by size:
3079 988 565 485 281 246
HindIII A'AGCT_T
Cuts at: 748 2519 748
Size: 1771 3873
HpaI GTT'AAC
Cuts at: 3234 3234
Size: 5644
KpnI G_GTAC'C
Cuts at: 2969 2969
Size: 5644
MfeI C'AATT_G
Cuts at: 3243 3243
Size: 5644
MluI A'CGCG_T
Cuts at: 5520 5520
Size: 5644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2814 2934 4758 4942 1305
Size: 1509 120 1824 184 2007
Fragments arranged by size:
2007 1824 1509 184 120
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3822
Fragments arranged by size:
3822 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3602 3961 4120 518
Size: 648 2436 359 159 2042
Fragments arranged by size:
2436 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3060 3737 4885 5130 1606
Size: 1454 677 1148 245 2120
Fragments arranged by size:
2120 1454 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5092
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5644
NotI GC'GGCC_GC
Cuts at: 3091 3091
Size: 5644
NsiI A_TGCA'T
Cuts at: 2442 2674 2442
Size: 232 5412
NspI r_CATG'y
Cuts at: 1132 1229 3002 4547 1132
Size: 97 1773 1545 2229
Fragments arranged by size:
2229 1773 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5644
PciI A'CATG_T
Cuts at: 4543 4543
Size: 5644
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5644
PfoI T'CCnGG_A
Cuts at: 2314 2314
Size: 5644
PmeI GTTT'AAAC
Cuts at: 2948 2948
Size: 5644
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 5226 5258 3713
Size: 32 1481 32 4099
Fragments arranged by size:
4099 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5644
PsiI TTA'TAA
Cuts at: 3214 3214
Size: 5644
PstI C_TGCA'G
Cuts at: 830 2448 2497 2996 830
Size: 1618 49 499 3478
Fragments arranged by size:
3478 1618 499 49
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5644
SalI G'TCGA_C
Cuts at: 1936 2986 1936
Size: 1050 4594
SbfI CC_TGCA'GG
Cuts at: 2996 2996
Size: 5644
ScaI AGT'ACT
Cuts at: 3892 5604 3892
Size: 1712 3932
SfcI C'TryA_G
Cuts at: 826 1045 2444 2493 2992 4127 4808 4999 826
Size: 219 1399 49 499 1135 681 191 1471
Fragments arranged by size:
1471 1399 1135 681 499 219 191 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5644
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5644
SmaI CCC'GGG
Cuts at: 1872 2971 1872
Size: 1099 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2116 3748 4649 4911 820
Size: 197 926 173 1632 901 262 1553
Fragments arranged by size:
1632 1553 926 901 262 197 173
SnaBI TAC'GTA
Cuts at: 493 3435 493
Size: 2942 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5644
SphI G_CATG'C
Cuts at: 1132 3002 1132
Size: 1870 3774
SspI AAT'ATT
Cuts at: 5 52 3568 5
Size: 47 3516 2081
Fragments arranged by size:
3516 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5644
StyI C'CwwG_G
Cuts at: 513 1065 3083 513
Size: 552 2018 3074
Fragments arranged by size:
3074 2018 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 3671 3688 3841 4026 4471 325
Size: 1255 450 1641 17 153 185 445 1498
Fragments arranged by size:
1641 1498 1255 450 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3890 5602
Size: 274 80 33 51 1443 204 1709 1712
Cuts at: 5602 96
Size: 138
Fragments arranged by size:
1712 1709 1443 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5604 5636 5604
Size: 32 5612
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5644
XbaI T'CTAG_A
Cuts at: 2980 2980
Size: 5644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2353 2801 2353
Size: 448 5196
XhoI C'TCGA_G
Cuts at: 1943 2116 1943
Size: 173 5471
XmaI C'CCGG_G
Cuts at: 1870 2969 1870
Size: 1099 4545
XmnI GAAnn'nnTTC
Cuts at: 3773 3773
Size: 5644
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3452 276
Size: 53 83 186 936 1918 2468
Fragments arranged by size:
2468 1918 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI