Restriction map for
pFN21AE1226
(Circular) (Six-base) MAPSORT of: pFN21AE1226.seq Check: 1420 from: 1 to: 5605
pFN21AE1226 5605 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2073 2073
Size: 5605
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3415 278
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2673 2948 1937
Size: 736 275 4594
Fragments arranged by size:
4594 736 275
Acc65I G'GTAC_C
Cuts at: 2279 2926 2279
Size: 647 4958
AclI AA'CG_TT
Cuts at: 1563 3732 4105 1563
Size: 2169 373 3063
Fragments arranged by size:
3063 2169 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3668 5052 5320 1632
Size: 2036 1384 268 1917
Fragments arranged by size:
2036 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5408
AflIII A'CryG_T
Cuts at: 1203 2204 4504 5481 1203
Size: 1001 2300 977 1327
Fragments arranged by size:
2300 1327 1001 977
AgeI A'CCGG_T
Cuts at: 4436 4436
Size: 5605
AhdI GACnn_n'nnGTC
Cuts at: 4334 4334
Size: 5605
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2528 1184
Size: 1344 4261
AlwNI CAG_nnn'CTG
Cuts at: 2894 3348 4920 2894
Size: 454 1572 3579
Fragments arranged by size:
3579 1572 454
ApaLI G'TGCA_C
Cuts at: 2261 3662 4818 2261
Size: 1401 1156 3048
Fragments arranged by size:
3048 1401 1156
ApoI r'AATT_y
Cuts at: 1493 2914 3140 3292 5401 1493
Size: 1421 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4159 160
Size: 872 3127 1606
Fragments arranged by size:
3127 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2094 2930 1870
Size: 73 127 24 836 4545
Fragments arranged by size:
4545 836 127 73 24
BamHI G'GATC_C
Cuts at: 2935 3302 2935
Size: 367 5238
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2279 2291 2504 2926
Size: 325 217 627 363 129 12 213 422
Cuts at: 2926 4381 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 422 363 325 217 213
129 12
BanII G_rGCy'C
Cuts at: 1391 2484 2540 2669 2841 2924 1391
Size: 1093 56 129 172 83 4072
Fragments arranged by size:
4072 1093 172 129 83 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3796 3830 1903
Size: 34 1859 34 3678
Fragments arranged by size:
3678 1859 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3497 4707 3497
Size: 1210 4395
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5605
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4216 136
Size: 107 122 71 631 605 2544 1525
Fragments arranged by size:
2544 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5600 5600
Size: 5605
BlpI GC'TnA_GC
Cuts at: 3022 3022
Size: 5605
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2265 3666 4822 1790
Size: 304 171 1401 1156 2573
Fragments arranged by size:
2573 1401 1156 304 171
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5605
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2367 3336 4294
Size: 416 53 83 399 24 950 969 958
Cuts at: 4294 442
Size: 1753
Fragments arranged by size:
1753 969 958 950 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5605
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2780 2860 4265 1239
Size: 1541 80 1405 2579
Fragments arranged by size:
2579 1541 1405 80
Bpu10I CC'TnA_GC
Cuts at: 2478 2799 2478
Size: 321 5284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3730 4595 4893 3730
Size: 865 298 4442
Fragments arranged by size:
4442 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4268 882
Size: 617 2769 2219
Fragments arranged by size:
2769 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2686 2740 3396 493
Size: 711 1482 54 656 2702
Fragments arranged by size:
2702 1482 711 656 54
BsaBI GATnn'nnATC
Cuts at: 5599 5599
Size: 5605
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3412 3794 4429 275
Size: 53 83 186 936 1879 382 635 1451
Fragments arranged by size:
1879 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4038 4436 4710 4857 5268 4038
Size: 398 274 147 411 4375
Fragments arranged by size:
4375 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2696 2726 2739 2769 1178
Size: 30 165 30 1293 30 13 30 4014
Fragments arranged by size:
4014 1293 165 30 30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2789 1186
Size: 1603 4002
BseYI C'CCAG_C
Cuts at: 2082 2460 4808 5304 2082
Size: 378 2348 496 2383
Fragments arranged by size:
2383 2348 496 378
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5032
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3055 3816 4844 1540
Size: 275 186 1054 761 1028 2301
Fragments arranged by size:
2301 1054 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 2484 2597 2841 3666 3751 4822 2265
Size: 219 113 244 825 85 1071 3048
Fragments arranged by size:
3048 1071 825 244 219 113 85
BsmI GAATG_Cn'
Cuts at: 3116 3209 3116
Size: 93 5512
BsmBI CGTCTCn'nnnn_
Cuts at: 2891 2891
Size: 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2265 2484 2540 2597 2669 2841
Size: 399 304 171 219 56 57 72 172
Cuts at: 2841 2924 3666 3751 4822 1391
Size: 83 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 304 219 172 171
85 83 72 57 56
BspEI T'CCGG_A
Cuts at: 5268 5268
Size: 5605
BspHI T'CATG_A
Cuts at: 3494 5224 3494
Size: 1730 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 2960 844
Size: 1229 887 3489
Fragments arranged by size:
3489 1229 887
BsrBI CCG'CTC
Cuts at: 1394 1823 2469 3492 1394
Size: 429 646 1023 3507
Fragments arranged by size:
3507 1023 646 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2187 4100 4274 65
Size: 1183 939 1913 174 1396
Fragments arranged by size:
1913 1396 1183 939 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4249 4436 1700
Size: 110 2439 187 2869
Fragments arranged by size:
2869 2439 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5605
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5605
BssSI C'ACGA_G
Cuts at: 3665 4677 3665
Size: 1012 4593
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5605
BstBI TT'CG_AA
Cuts at: 4409 4409
Size: 5605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5605
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2161
Size: 5605
BstYI r'GATC_y
Cuts at: 1972 2124 2431 2543 2935 3302 3687 3704 5156
Size: 152 307 112 392 367 385 17 1452
Cuts at: 5156 5242 5254 5600 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 392 385 367 346 307 152
112 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2128 2146 2572 2628 513
Size: 552 939 124 18 426 56 3490
Fragments arranged by size:
3490 939 552 426 124 56 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4866
Fragments arranged by size:
4866 683 56
BtsI GCAGTG_nn'
Cuts at: 2445 2725 2808 3121 3915 3935 2445
Size: 280 83 313 794 20 4115
Fragments arranged by size:
4115 794 313 280 83 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5605
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5570
DraI TTT'AAA
Cuts at: 2909 2909
Size: 5605
DraIII CAC_nnn'GTG
Cuts at: 2720 2720
Size: 5605
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4612 809
Size: 507 3296 1802
Fragments arranged by size:
3296 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2379 2442 3052
Size: 54 1425 50 275 18 549 63 610
Cuts at: 3052 3941 5570 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 610 549 275 63 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 1537
Size: 275 1240 4090
Fragments arranged by size:
4090 1240 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2489 3535 1353
Size: 1136 1046 3423
Fragments arranged by size:
3423 1136 1046
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4192 4566 4712 387
Size: 1064 138 2603 374 146 1280
Fragments arranged by size:
2603 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2482 2839 2482
Size: 357 5248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2780 2860 3668 4265 5052 5320 1239
Size: 393 1148 80 808 597 787 268 1524
Fragments arranged by size:
1524 1148 808 787 597 393 268 80
EcoRI G'AATT_C
Cuts at: 2914 3292 2914
Size: 378 5227
FspI TGC'GCA
Cuts at: 4111 4111
Size: 5605
HaeII r_GCGC'y
Cuts at: 1678 4752 1678
Size: 3074 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2347 2379 2418 2450 2513
Size: 32 135 32 775 32 39 32 63
Cuts at: 2513 2545 2696 2728 4246 4278 4320 4352 1373
Size: 32 151 32 1518 32 42 32 2626
Fragments arranged by size:
2626 1518 775 151 135 63 42 39
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2949 3195 669
Size: 988 281 1011 246 3079
Fragments arranged by size:
3079 1011 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5605
HpaI GTT'AAC
Cuts at: 3195 3195
Size: 5605
KpnI G_GTAC'C
Cuts at: 2283 2930 2283
Size: 647 4958
MfeI C'AATT_G
Cuts at: 3204 3204
Size: 5605
MluI A'CGCG_T
Cuts at: 2204 5481 2204
Size: 3277 2328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4719 4903 1305
Size: 3414 184 2007
Fragments arranged by size:
3414 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2381 2444 10
Size: 54 1425 343 549 63 3171
Fragments arranged by size:
3171 1425 549 343 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 2649 3563 3922 4081 518
Size: 648 1092 391 914 359 159 2042
Fragments arranged by size:
2042 1092 914 648 391 359 159
MspA1I CmG'CkG
Cuts at: 1606 2198 2332 2368 3021 3698 4846 5091 1606
Size: 592 134 36 653 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 653 592 245 134 36
NcoI C'CATG_G
Cuts at: 513 1065 2004 2128 513
Size: 552 939 124 3990
Fragments arranged by size:
3990 939 552 124
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5605
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5605
NotI GC'GGCC_GC
Cuts at: 3052 3052
Size: 5605
NspI r_CATG'y
Cuts at: 1132 1229 2711 2963 4508 1132
Size: 97 1482 252 1545 2229
Fragments arranged by size:
2229 1545 1482 252 97
PasI CC'CwG_GG
Cuts at: 1348 2047 1348
Size: 699 4906
PciI A'CATG_T
Cuts at: 4504 4504
Size: 5605
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5605
PfoI T'CCnGG_A
Cuts at: 2757 2876 2757
Size: 119 5486
PmeI GTTT'AAAC
Cuts at: 2909 2909
Size: 5605
PmlI CAC'GTG
Cuts at: 2686 2686
Size: 5605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 5187 5219 3674
Size: 32 1481 32 4060
Fragments arranged by size:
4060 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5605
PsiI TTA'TAA
Cuts at: 3175 3175
Size: 5605
PstI C_TGCA'G
Cuts at: 830 2016 2124 2197 2517 2827 2957 830
Size: 1186 108 73 320 310 130 3478
Fragments arranged by size:
3478 1186 320 310 130 108 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5605
PvuII CAG'CTG
Cuts at: 2198 2368 2198
Size: 170 5435
SacI G_AGCT'C
Cuts at: 2484 2841 2484
Size: 357 5248
SalI G'TCGA_C
Cuts at: 1936 2947 1936
Size: 1011 4594
SapI GCTCTTCn'nnn_
Cuts at: 2489 2489
Size: 5605
SbfI CC_TGCA'GG
Cuts at: 2517 2957 2517
Size: 440 5165
ScaI AGT'ACT
Cuts at: 3853 5565 3853
Size: 1712 3893
SexAI A'CCwGG_T
Cuts at: 2312 2312
Size: 5605
SfcI C'TryA_G
Cuts at: 826 1045 2012 2120 2193 2494 2513 2670 2823
Size: 219 967 108 73 301 19 157 153
Cuts at: 2823 2953 4088 4769 4960 826
Size: 130 1135 681 191 1471
Fragments arranged by size:
1471 1135 967 681 301 219 191 157
153 130 108 73 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5605
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5605
SmaI CCC'GGG
Cuts at: 1872 2096 2932 1872
Size: 224 836 4545
Fragments arranged by size:
4545 836 224
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 4610 4872 820
Size: 197 926 1766 901 262 1553
Fragments arranged by size:
1766 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3396 493
Size: 2903 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5605
SphI G_CATG'C
Cuts at: 1132 2963 1132
Size: 1831 3774
SspI AAT'ATT
Cuts at: 5 52 3529 5
Size: 47 3477 2081
Fragments arranged by size:
3477 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5605
StyI C'CwwG_G
Cuts at: 513 1065 2004 2128 3044 513
Size: 552 939 124 916 3074
Fragments arranged by size:
3074 939 916 552 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 2612 2657 3632 3649 3802 3987
Size: 1255 480 552 45 975 17 153 185
Cuts at: 3987 4432 325
Size: 445 1498
Fragments arranged by size:
1498 1255 975 552 480 445 185 153
45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3851 5563 96
Size: 274 80 33 51 1443 1874 1712 138
Fragments arranged by size:
1874 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5565 5597 5565
Size: 32 5573
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5605
XbaI T'CTAG_A
Cuts at: 2941 2941
Size: 5605
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5605
XmaI C'CCGG_G
Cuts at: 1870 2094 2930 1870
Size: 224 836 4545
Fragments arranged by size:
4545 836 224
XmnI GAAnn'nnTTC
Cuts at: 3734 3734
Size: 5605
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3413 276
Size: 53 83 186 936 1879 2468
Fragments arranged by size:
2468 1879 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI XcmI