Restriction map for
pFN21AE1225
(Circular) (Six-base) MAPSORT of: pFN21AE1225.seq Check: 2758 from: 1 to: 5566
pFN21AE1225 5566 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3376 278
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2909 1937
Size: 972 4594
Acc65I G'GTAC_C
Cuts at: 2887 2887
Size: 5566
AclI AA'CG_TT
Cuts at: 1563 3693 4066 1563
Size: 2130 373 3063
Fragments arranged by size:
3063 2130 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2361 2532 3629 5013 5281 1632
Size: 729 171 1097 1384 268 1917
Fragments arranged by size:
1917 1384 1097 729 268 171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5369
AflIII A'CryG_T
Cuts at: 1203 4465 5442 1203
Size: 3262 977 1327
Fragments arranged by size:
3262 1327 977
AgeI A'CCGG_T
Cuts at: 4397 4397
Size: 5566
AhdI GACnn_n'nnGTC
Cuts at: 4295 4295
Size: 5566
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5566
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5566
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2285 2317 2285
Size: 32 5534
AlwNI CAG_nnn'CTG
Cuts at: 3309 4881 3309
Size: 1572 3994
ApaLI G'TGCA_C
Cuts at: 3623 4779 3623
Size: 1156 4410
ApoI r'AATT_y
Cuts at: 1493 2875 3101 3253 5362 1493
Size: 1382 226 152 2109 1697
Fragments arranged by size:
2109 1697 1382 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4120 160
Size: 872 3088 1606
Fragments arranged by size:
3088 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
AvaI C'yCGr_G
Cuts at: 1870 1943 2891 1870
Size: 73 948 4545
Fragments arranged by size:
4545 948 73
BamHI G'GATC_C
Cuts at: 2896 3263 2896
Size: 367 5199
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2429 2477 2709 2887 4342
Size: 325 217 627 642 48 232 178 1455
Cuts at: 4342 618
Size: 1842
Fragments arranged by size:
1842 1455 642 627 325 232 217 178
48
BanII G_rGCy'C
Cuts at: 1391 2130 2241 2751 2885 1391
Size: 739 111 510 134 4072
Fragments arranged by size:
4072 739 510 134 111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2349 928
Size: 971 450 4145
Fragments arranged by size:
4145 971 450
BbvCI CC'TCA_GC
Cuts at: 2204 2204
Size: 5566
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3757 3791 1903
Size: 34 1820 34 3678
Fragments arranged by size:
3678 1820 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3458 4668 3458
Size: 1210 4356
BclI T'GATC_A
Cuts at: 1548 2791 2820 1548
Size: 1243 29 4294
Fragments arranged by size:
4294 1243 29
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4177 136
Size: 107 122 71 631 605 2505 1525
Fragments arranged by size:
2505 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2053 5561 2053
Size: 3508 2058
BlpI GC'TnA_GC
Cuts at: 2010 2983 2010
Size: 973 4593
Bme1580I G_kGCm'C
Cuts at: 1790 2432 2480 2845 3627 4783 1790
Size: 642 48 365 782 1156 2573
Fragments arranged by size:
2573 1156 782 642 365 48
BmgBI CAC'GTC
Cuts at: 1318 2473 1318
Size: 1155 4411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2470 2632 2840
Size: 416 53 83 399 24 1053 162 208
Cuts at: 2840 3297 4255 442
Size: 457 958 1753
Fragments arranged by size:
1753 1053 958 457 416 399 208 162
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2102 4226 1239
Size: 845 18 2124 2579
Fragments arranged by size:
2579 2124 845 18
Bpu10I CC'TnA_GC
Cuts at: 2204 2204
Size: 5566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3691 4556 4854 3691
Size: 865 298 4403
Fragments arranged by size:
4403 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2173 4229 882
Size: 617 674 2056 2219
Fragments arranged by size:
2219 2056 674 617
BsaAI yAC'GTr
Cuts at: 493 1204 3357 493
Size: 711 2153 2702
Fragments arranged by size:
2702 2153 711
BsaBI GATnn'nnATC
Cuts at: 5560 5560
Size: 5566
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2755 3373 3755 4390
Size: 53 83 186 936 1222 618 382 635
Cuts at: 4390 275
Size: 1451
Fragments arranged by size:
1451 1222 936 635 618 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3999 4397 4671 4818 5229 3999
Size: 398 274 147 411 4336
Fragments arranged by size:
4336 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5341
Fragments arranged by size:
5341 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2365 2610 1186
Size: 1179 245 4142
Fragments arranged by size:
4142 1179 245
BseYI C'CCAG_C
Cuts at: 4769 5265 4769
Size: 496 5070
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4993
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2452 3016 3777 4805 1540
Size: 275 186 451 564 761 1028 2301
Fragments arranged by size:
2301 1028 761 564 451 275 186
BsiHKAI G_wGCw'C
Cuts at: 2016 3627 3712 4783 2016
Size: 1611 85 1071 2799
Fragments arranged by size:
2799 1611 1071 85
BsmI GAATG_Cn'
Cuts at: 3077 3170 3077
Size: 93 5473
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2130 2241 2432 2480 2751 2845
Size: 399 226 114 111 191 48 271 94
Cuts at: 2845 2885 3627 3712 4783 1391
Size: 40 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 271 226 191 114
111 94 85 48 40
BspEI T'CCGG_A
Cuts at: 5229 5229
Size: 5566
BspHI T'CATG_A
Cuts at: 3455 5185 3455
Size: 1730 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2921 844
Size: 2077 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3453 1394
Size: 429 1630 3507
Fragments arranged by size:
3507 1630 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2682 4061 4235 65
Size: 1183 1434 1379 174 1396
Fragments arranged by size:
1434 1396 1379 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4210 4397 1700
Size: 110 2400 187 2869
Fragments arranged by size:
2869 2400 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5566
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5566
BssSI C'ACGA_G
Cuts at: 3626 4638 3626
Size: 1012 4554
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5566
BstBI TT'CG_AA
Cuts at: 4370 4370
Size: 5566
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5566
BstYI r'GATC_y
Cuts at: 1972 2053 2896 3263 3648 3665 5117 5203 5215
Size: 81 843 367 385 17 1452 86 12
Cuts at: 5215 5561 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 843 385 367 346 86 81
17 12
Bsu36I CC'TnA_GG
Cuts at: 2348 2348
Size: 5566
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4075
Fragments arranged by size:
4075 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4827
Fragments arranged by size:
4827 683 56
BtsI GCAGTG_nn'
Cuts at: 2409 3082 3876 3896 2409
Size: 673 794 20 4079
Fragments arranged by size:
4079 794 673 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5531
DraI TTT'AAA
Cuts at: 2870 2870
Size: 5566
DraIII CAC_nnn'GTG
Cuts at: 2650 2650
Size: 5566
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4573 809
Size: 507 3257 1802
Fragments arranged by size:
3257 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2449 3013 3902
Size: 54 1425 50 275 18 619 564 889
Cuts at: 3902 5531 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 619 564 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2449 3013 1537
Size: 275 637 564 4090
Fragments arranged by size:
4090 637 564 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3496 1353
Size: 2143 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4153 4527 4673 387
Size: 1064 138 2564 374 146 1280
Fragments arranged by size:
2564 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2102 2361 2532 3629 4226 5013
Size: 393 452 18 259 171 1097 597 787
Cuts at: 5013 5281 1239
Size: 268 1524
Fragments arranged by size:
1524 1097 787 597 452 393 268 259
171 18
EcoRI G'AATT_C
Cuts at: 2875 3253 2875
Size: 378 5188
FspI TGC'GCA
Cuts at: 4072 4072
Size: 5566
HaeII r_GCGC'y
Cuts at: 1678 2213 4713 1678
Size: 535 2500 2531
Fragments arranged by size:
2531 2500 535
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2025 2057 4207 4239 4281
Size: 32 135 32 453 32 2150 32 42
Cuts at: 4281 4313 1373
Size: 32 2626
Fragments arranged by size:
2626 2150 453 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2521 2910 3156 669
Size: 988 281 583 389 246 3079
Fragments arranged by size:
3079 988 583 389 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5566
HpaI GTT'AAC
Cuts at: 3156 3156
Size: 5566
KpnI G_GTAC'C
Cuts at: 2891 2891
Size: 5566
MfeI C'AATT_G
Cuts at: 3165 3165
Size: 5566
MluI A'CGCG_T
Cuts at: 5442 5442
Size: 5566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4680 4864 1305
Size: 3375 184 2007
Fragments arranged by size:
3375 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3744
Fragments arranged by size:
3744 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2775 3524 3883 4042 518
Size: 648 1609 749 359 159 2042
Fragments arranged by size:
2042 1609 749 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2834 2982 3659 4807 5052 1606
Size: 1228 148 677 1148 245 2120
Fragments arranged by size:
2120 1228 1148 677 245 148
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4075
Fragments arranged by size:
4075 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5566
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5566
NotI GC'GGCC_GC
Cuts at: 3013 3013
Size: 5566
NspI r_CATG'y
Cuts at: 1132 1229 2924 4469 1132
Size: 97 1695 1545 2229
Fragments arranged by size:
2229 1695 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5566
PciI A'CATG_T
Cuts at: 4465 4465
Size: 5566
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5566
PfoI T'CCnGG_A
Cuts at: 2661 2661
Size: 5566
PmeI GTTT'AAAC
Cuts at: 2870 2870
Size: 5566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2797 2829 3635 3667 5148 5180 2797
Size: 32 806 32 1481 32 3183
Fragments arranged by size:
3183 1481 806 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5566
PsiI TTA'TAA
Cuts at: 3136 3136
Size: 5566
PstI C_TGCA'G
Cuts at: 830 2918 830
Size: 2088 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5566
SalI G'TCGA_C
Cuts at: 1936 2908 1936
Size: 972 4594
SbfI CC_TGCA'GG
Cuts at: 2918 2918
Size: 5566
ScaI AGT'ACT
Cuts at: 3814 5526 3814
Size: 1712 3854
SfcI C'TryA_G
Cuts at: 826 1045 2914 4049 4730 4921 826
Size: 219 1869 1135 681 191 1471
Fragments arranged by size:
1869 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5566
SmaI CCC'GGG
Cuts at: 1872 2893 1872
Size: 1021 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3670 4571 4833 820
Size: 197 926 1727 901 262 1553
Fragments arranged by size:
1727 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3357 493
Size: 2864 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5566
SphI G_CATG'C
Cuts at: 1132 2924 1132
Size: 1792 3774
SspI AAT'ATT
Cuts at: 5 52 3490 5
Size: 47 3438 2081
Fragments arranged by size:
3438 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5566
StyI C'CwwG_G
Cuts at: 513 1065 2004 2270 3005 513
Size: 552 939 266 735 3074
Fragments arranged by size:
3074 939 735 552 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2617 2697 3593 3610 3763 3948 4393
Size: 1255 1037 80 896 17 153 185 445
Cuts at: 4393 325
Size: 1498
Fragments arranged by size:
1498 1255 1037 896 445 185 153 80
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 3812 5524
Size: 274 80 33 51 1443 219 1616 1712
Cuts at: 5524 96
Size: 138
Fragments arranged by size:
1712 1616 1443 274 219 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5526 5558 5526
Size: 32 5534
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5566
XbaI T'CTAG_A
Cuts at: 2902 2902
Size: 5566
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5566
XmaI C'CCGG_G
Cuts at: 1870 2891 1870
Size: 1021 4545
XmnI GAAnn'nnTTC
Cuts at: 3695 3695
Size: 5566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3374 276
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI