Restriction map for
pFN21AE1222
(Circular) (Six-base) MAPSORT of: pFN21AE1222.seq Check: 8952 from: 1 to: 5440
pFN21AE1222 5440 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3250 278
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2783 1937
Size: 846 4594
Acc65I G'GTAC_C
Cuts at: 2637 2761 2637
Size: 124 5316
AclI AA'CG_TT
Cuts at: 1563 3567 3940 1563
Size: 2004 373 3063
Fragments arranged by size:
3063 2004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3503 4887 5155 1632
Size: 1871 1384 268 1917
Fragments arranged by size:
1917 1871 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 4339 5316 1203
Size: 3136 977 1327
Fragments arranged by size:
3136 1327 977
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 2577 4169 2577
Size: 1592 3848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5440
AlwNI CAG_nnn'CTG
Cuts at: 2051 2715 3183 4755 2051
Size: 664 468 1572 2736
Fragments arranged by size:
2736 1572 664 468
ApaI G_GGCC'C
Cuts at: 2291 2291
Size: 5440
ApaLI G'TGCA_C
Cuts at: 3497 4653 3497
Size: 1156 4284
ApoI r'AATT_y
Cuts at: 1493 2749 2975 3127 5236 1493
Size: 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3994 160
Size: 872 2962 1606
Fragments arranged by size:
2962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2765 1870
Size: 73 822 4545
Fragments arranged by size:
4545 822 73
BamHI G'GATC_C
Cuts at: 2114 2770 3137 2114
Size: 656 367 4417
Fragments arranged by size:
4417 656 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2637 2761 4216 618
Size: 325 217 627 850 124 1455 1842
Fragments arranged by size:
1842 1455 850 627 325 217 124
BanII G_rGCy'C
Cuts at: 1391 2239 2291 2325 2377 2393 2652 2759 1391
Size: 848 52 34 52 16 259 107 4072
Fragments arranged by size:
4072 848 259 107 52 52 34 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2410 928
Size: 971 511 3958
Fragments arranged by size:
3958 971 511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3631 3665 1903
Size: 34 1694 34 3678
Fragments arranged by size:
3678 1694 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2108 2255 3332 4542 2108
Size: 147 1077 1210 3006
Fragments arranged by size:
3006 1210 1077 147
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5440
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2130 2175 4051
Size: 107 122 71 631 605 458 45 1876
Cuts at: 4051 136
Size: 1525
Fragments arranged by size:
1876 1525 631 605 458 122 107 71
45
BglII A'GATC_T
Cuts at: 5435 5435
Size: 5440
BlpI GC'TnA_GC
Cuts at: 2857 2857
Size: 5440
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2291 3501 4657 1790
Size: 228 273 1210 1156 2573
Fragments arranged by size:
2573 1210 1156 273 228
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3171 4129 442
Size: 416 53 83 399 24 1754 958 1753
Fragments arranged by size:
1754 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5440
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2307 2307
Size: 5440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 2214 2253 2277 2407 2610 4100 1239
Size: 918 57 39 24 130 203 1490 2579
Fragments arranged by size:
2579 1490 918 203 130 57 39 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3565 4430 4728 3565
Size: 865 298 4277
Fragments arranged by size:
4277 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2154 4103 882
Size: 617 655 1949 2219
Fragments arranged by size:
2219 1949 655 617
BsaAI yAC'GTr
Cuts at: 493 1204 3231 493
Size: 711 2027 2702
Fragments arranged by size:
2702 2027 711
BsaBI GATnn'nnATC
Cuts at: 5434 5434
Size: 5440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3247 3629 4264 275
Size: 53 83 186 936 1714 382 635 1451
Fragments arranged by size:
1714 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3873 4271 4545 4692 5103 3873
Size: 398 274 147 411 4210
Fragments arranged by size:
4210 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2134 2164 2400 2430 1178
Size: 30 165 30 731 30 236 30 4188
Fragments arranged by size:
4188 731 236 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2333 1186
Size: 1147 4293
BseYI C'CCAG_C
Cuts at: 2582 4643 5139 2582
Size: 2061 496 2883
Fragments arranged by size:
2883 2061 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4867
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2485 2890 3651 4679 1540
Size: 275 186 484 405 761 1028 2301
Fragments arranged by size:
2301 1028 761 484 405 275 186
BsiHKAI G_wGCw'C
Cuts at: 2325 3501 3586 4657 2325
Size: 1176 85 1071 3108
Fragments arranged by size:
3108 1176 1071 85
BsmI GAATG_Cn'
Cuts at: 2951 3044 2951
Size: 93 5347
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2239 2291 2325 2377 2393 2652
Size: 399 228 221 52 34 52 16 259
Cuts at: 2652 2759 3501 3586 4657 1391
Size: 107 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 259 228 221 107
85 52 52 34 16
BspEI T'CCGG_A
Cuts at: 5103 5103
Size: 5440
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2645 2795 844
Size: 1801 150 3489
Fragments arranged by size:
3489 1801 150
BsrBI CCG'CTC
Cuts at: 1394 1823 3327 1394
Size: 429 1504 3507
Fragments arranged by size:
3507 1504 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2434 2487 2523 3935 4109 65
Size: 1183 1186 53 36 1412 174 1396
Fragments arranged by size:
1412 1396 1186 1183 174 53 36
BsrFI r'CCGG_y
Cuts at: 1700 1810 4084 4271 1700
Size: 110 2274 187 2869
Fragments arranged by size:
2869 2274 187 110
BsrGI T'GTAC_A
Cuts at: 96 2685 96
Size: 2589 2851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5440
BssSI C'ACGA_G
Cuts at: 3500 4512 3500
Size: 1012 4428
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5440
BstBI TT'CG_AA
Cuts at: 4244 4244
Size: 5440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5440
BstYI r'GATC_y
Cuts at: 1972 2114 2770 3137 3522 3539 4991 5077 5089
Size: 142 656 367 385 17 1452 86 12
Cuts at: 5089 5435 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 656 385 367 346 142 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2119 2181 513
Size: 552 1054 62 3772
Fragments arranged by size:
3772 1054 552 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4701
Fragments arranged by size:
4701 683 56
BtsI GCAGTG_nn'
Cuts at: 2678 2705 2956 3750 3770 2678
Size: 27 251 794 20 4348
Fragments arranged by size:
4348 794 251 27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5405
DraI TTT'AAA
Cuts at: 2744 2744
Size: 5440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4447 809
Size: 507 3131 1802
Fragments arranged by size:
3131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2482 2887 3776
Size: 54 1425 50 275 18 652 405 889
Cuts at: 3776 5405 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 652 405 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2482 2887 1537
Size: 275 670 405 4090
Fragments arranged by size:
4090 670 405 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3370 1353
Size: 2017 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4027 4401 4547 387
Size: 1064 138 2438 374 146 1280
Fragments arranged by size:
2438 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2323 2323
Size: 5440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2214 2253 2277 2407 2610 3503
Size: 393 525 57 39 24 130 203 893
Cuts at: 3503 4100 4887 5155 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 893 787 597 525 393 268 203
130 57 39 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 2573
Size: 5440
EcoO109I rG'GnC_Cy
Cuts at: 2177 2287 2288 2579 2613 2177
Size: 110 1 291 34 5004
Fragments arranged by size:
5004 291 110 34 1
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
FspI TGC'GCA
Cuts at: 3946 3946
Size: 5440
HaeII r_GCGC'y
Cuts at: 1678 4587 1678
Size: 2909 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2136 2168 2340 2372 2400
Size: 32 135 32 564 32 172 32 28
Cuts at: 2400 2432 2563 2595 2721 2753 4081 4113 4155
Size: 32 131 32 126 32 1328 32 42
Cuts at: 4155 4187 1373
Size: 32 2626
Fragments arranged by size:
2626 1328 564 172 135 131 126 42
32 32 32 32 32 32 32 32
32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2784 3030 669
Size: 988 281 846 246 3079
Fragments arranged by size:
3079 988 846 281 246
HindIII A'AGCT_T
Cuts at: 748 2530 748
Size: 1782 3658
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KpnI G_GTAC'C
Cuts at: 2641 2765 2641
Size: 124 5316
MfeI C'AATT_G
Cuts at: 3039 3039
Size: 5440
MluI A'CGCG_T
Cuts at: 5316 5316
Size: 5440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2065 4554 4738 1305
Size: 760 2489 184 2007
Fragments arranged by size:
2489 2007 760 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3618
Fragments arranged by size:
3618 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2313 3398 3757 3916 518
Size: 648 873 274 1085 359 159 2042
Fragments arranged by size:
2042 1085 873 648 359 274 159
MspA1I CmG'CkG
Cuts at: 1606 2856 3533 4681 4926 1606
Size: 1250 677 1148 245 2120
Fragments arranged by size:
2120 1250 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2181 513
Size: 552 1116 3772
Fragments arranged by size:
3772 1116 552
NdeI CA'TA_TG
Cuts at: 387 2554 387
Size: 2167 3273
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5440
NotI GC'GGCC_GC
Cuts at: 2482 2887 2482
Size: 405 5035
NspI r_CATG'y
Cuts at: 1132 1229 2469 2798 4343 1132
Size: 97 1240 329 1545 2229
Fragments arranged by size:
2229 1545 1240 329 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5440
PciI A'CATG_T
Cuts at: 4339 4339
Size: 5440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5440
PfoI T'CCnGG_A
Cuts at: 2110 2212 2110
Size: 102 5338
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2579 2613 2579
Size: 34 5406
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5440
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PspOMI G'GGCC_C
Cuts at: 2287 2287
Size: 5440
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2705 2737 2705
Size: 32 5408
PstI C_TGCA'G
Cuts at: 830 2364 2388 2792 830
Size: 1534 24 404 3478
Fragments arranged by size:
3478 1534 404 24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5440
SacI G_AGCT'C
Cuts at: 2325 2325
Size: 5440
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SanDI GG'GwC_CC
Cuts at: 2579 2613 2579
Size: 34 5406
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SfcI C'TryA_G
Cuts at: 826 1045 2360 2384 2788 3923 4604 4795 826
Size: 219 1315 24 404 1135 681 191 1471
Fragments arranged by size:
1471 1315 1135 681 404 219 191 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 2175 2175
Size: 5440
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2767 1872
Size: 895 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3544 4445 4707 820
Size: 197 926 1601 901 262 1553
Fragments arranged by size:
1601 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2798 1132
Size: 1666 3774
SspI AAT'ATT
Cuts at: 5 52 3364 5
Size: 47 3312 2081
Fragments arranged by size:
3312 2081 47
StuI AGG'CCT
Cuts at: 1517 2568 1517
Size: 1051 4389
StyI C'CwwG_G
Cuts at: 513 1065 2181 2498 2879 513
Size: 552 1116 317 381 3074
Fragments arranged by size:
3074 1116 552 381 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3467 3484 3637 3822 4267 325
Size: 1255 1887 17 153 185 445 1498
Fragments arranged by size:
1887 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2544 2685 2721
Size: 274 80 33 51 1443 567 141 36
Cuts at: 2721 3686 5398 96
Size: 965 1712 138
Fragments arranged by size:
1712 1443 965 567 274 141 138 80
51 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2134 2166 5400 5432 2134
Size: 32 3234 32 2142
Fragments arranged by size:
3234 2142 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5440
XbaI T'CTAG_A
Cuts at: 2311 2776 2311
Size: 465 4975
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5440
XmaI C'CCGG_G
Cuts at: 1870 2765 1870
Size: 895 4545
XmnI GAAnn'nnTTC
Cuts at: 2417 3569 2417
Size: 1152 4288
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3248 276
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI SacI SalI SanDI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PvuII RsrII SacII
SapI SexAI SfoI SrfI SwaI XcmI