Restriction map for
pFN21AE1221
(Circular) (Six-base) MAPSORT of: pFN21AE1221.seq Check: 1218 from: 1 to: 5434
pFN21AE1221 5434 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2444 2617 2444
Size: 173 5261
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3244 278
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2454 2481 2777 1937
Size: 517 27 296 4594
Fragments arranged by size:
4594 517 296 27
Acc65I G'GTAC_C
Cuts at: 2755 2755
Size: 5434
AclI AA'CG_TT
Cuts at: 1563 3561 3934 1563
Size: 1998 373 3063
Fragments arranged by size:
3063 1998 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2305 3497 4881 5149 1632
Size: 673 1192 1384 268 1917
Fragments arranged by size:
1917 1384 1192 673 268
AfeI AGC'GCT
Cuts at: 2333 2333
Size: 5434
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5237
AflIII A'CryG_T
Cuts at: 1203 4333 5310 1203
Size: 3130 977 1327
Fragments arranged by size:
3130 1327 977
AgeI A'CCGG_T
Cuts at: 4265 4265
Size: 5434
AhdI GACnn_n'nnGTC
Cuts at: 4163 4163
Size: 5434
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5434
AlwNI CAG_nnn'CTG
Cuts at: 2085 3177 4749 2085
Size: 1092 1572 2770
Fragments arranged by size:
2770 1572 1092
ApaI G_GGCC'C
Cuts at: 2277 2277
Size: 5434
ApaLI G'TGCA_C
Cuts at: 3491 4647 3491
Size: 1156 4278
ApoI r'AATT_y
Cuts at: 1493 2743 2969 3121 5230 1493
Size: 1250 226 152 2109 1697
Fragments arranged by size:
2109 1697 1250 226 152
AscI GG'CGCG_CC
Cuts at: 2175 2175
Size: 5434
AseI AT'TA_AT
Cuts at: 160 1032 3988 160
Size: 872 2956 1606
Fragments arranged by size:
2956 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
AvaI C'yCGr_G
Cuts at: 1870 1943 2089 2180 2232 2243 2578 2759 1870
Size: 73 146 91 52 11 335 181 4545
Fragments arranged by size:
4545 335 181 146 91 73 52 11
BamHI G'GATC_C
Cuts at: 2764 3131 2764
Size: 367 5067
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2094 2302 2347 2443 2755
Size: 325 217 627 307 208 45 96 312
Cuts at: 2755 4210 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 325 312 307 217 208
96 45
BanII G_rGCy'C
Cuts at: 1391 2188 2277 2753 1391
Size: 797 89 476 4072
Fragments arranged by size:
4072 797 476 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2510 2544 3625 3659 1903
Size: 34 573 34 1081 34 3678
Fragments arranged by size:
3678 1081 573 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3326 4536 3326
Size: 1210 4224
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2109 2127 2154
Size: 107 122 71 631 605 437 18 27
Cuts at: 2154 2163 2172 2281 4045 136
Size: 9 9 109 1764 1525
Fragments arranged by size:
1764 1525 631 605 437 122 109 107
71 27 18 9 9
BglII A'GATC_T
Cuts at: 5429 5429
Size: 5434
BlpI GC'TnA_GC
Cuts at: 2851 2851
Size: 5434
Bme1580I G_kGCm'C
Cuts at: 1790 2097 2277 3495 4651 1790
Size: 307 180 1218 1156 2573
Fragments arranged by size:
2573 1218 1156 307 180
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2717 3165 4123
Size: 416 53 83 399 24 1300 448 958
Cuts at: 4123 442
Size: 1753
Fragments arranged by size:
1753 1300 958 448 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2621 4094 1239
Size: 1382 1473 2579
Fragments arranged by size:
2579 1473 1382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3559 4424 4722 3559
Size: 865 298 4271
Fragments arranged by size:
4271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4097 882
Size: 617 2598 2219
Fragments arranged by size:
2598 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3225 493
Size: 711 2021 2702
Fragments arranged by size:
2702 2021 711
BsaBI GATnn'nnATC
Cuts at: 5428 5428
Size: 5434
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2415 3241 3623 4258
Size: 53 83 186 936 882 826 382 635
Cuts at: 4258 275
Size: 1451
Fragments arranged by size:
1451 936 882 826 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2687 3867 4265 4539 4686 5097 2687
Size: 1180 398 274 147 411 3024
Fragments arranged by size:
3024 1180 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5209
Fragments arranged by size:
5209 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2006 2268 1186
Size: 820 262 4352
Fragments arranged by size:
4352 820 262
BseYI C'CCAG_C
Cuts at: 2078 4637 5133 2078
Size: 2559 496 2379
Fragments arranged by size:
2559 2379 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2645 1163
Size: 573 909 3952
Fragments arranged by size:
3952 909 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 2167 2383 2884 3645 4673
Size: 275 186 45 121 216 501 761 1028
Cuts at: 4673 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 501 275 216 186 121
45
BsiHKAI G_wGCw'C
Cuts at: 2300 3495 3580 4651 2300
Size: 1195 85 1071 3083
Fragments arranged by size:
3083 1195 1071 85
BsmI GAATG_Cn'
Cuts at: 2945 3038 2945
Size: 93 5341
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2097 2188 2277 2300 2753 3495 3580
Size: 399 307 91 89 23 453 742 85
Cuts at: 3580 4651 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 453 399 307 91 89
85 23
BspEI T'CCGG_A
Cuts at: 2687 5097 2687
Size: 2410 3024
BspHI T'CATG_A
Cuts at: 3323 5053 3323
Size: 1730 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2444 2617 2789 844
Size: 1600 173 172 3489
Fragments arranged by size:
3489 1600 173 172
BsrBI CCG'CTC
Cuts at: 1394 1823 3321 1394
Size: 429 1498 3507
Fragments arranged by size:
3507 1498 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2526 3929 4103 65
Size: 1183 1278 1403 174 1396
Fragments arranged by size:
1403 1396 1278 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 2423 4078 4265 1700
Size: 110 235 378 1655 187 2869
Fragments arranged by size:
2869 1655 378 235 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5434
BssHII G'CGCG_C
Cuts at: 1925 2175 1925
Size: 250 5184
BssSI C'ACGA_G
Cuts at: 3494 4506 3494
Size: 1012 4422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5434
BstBI TT'CG_AA
Cuts at: 4238 4238
Size: 5434
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5434
BstXI CCAn_nnnn'nTGG
Cuts at: 2447 2447
Size: 5434
BstYI r'GATC_y
Cuts at: 1972 2073 2764 3131 3516 3533 4985 5071 5083
Size: 101 691 367 385 17 1452 86 12
Cuts at: 5083 5429 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 691 385 367 346 101 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2607 2607
Size: 5434
BtgI C'CryG_G
Cuts at: 513 1065 2125 2268 2589 513
Size: 552 1060 143 321 3358
Fragments arranged by size:
3358 1060 552 321 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2166 487
Size: 683 56 940 3755
Fragments arranged by size:
3755 940 683 56
BtsI GCAGTG_nn'
Cuts at: 2950 3744 3764 2950
Size: 794 20 4620
Fragments arranged by size:
4620 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5399
DraI TTT'AAA
Cuts at: 2738 2738
Size: 5434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4441 809
Size: 507 3125 1802
Fragments arranged by size:
3125 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2155 2164
Size: 54 1425 50 275 18 213 112 9
Cuts at: 2164 2881 3770 5399 8
Size: 717 889 1629 43
Fragments arranged by size:
1629 1425 889 717 275 213 112 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2043 2164 2881 1537
Size: 275 231 121 717 4090
Fragments arranged by size:
4090 717 275 231 121
EarI CTCTTCn'nnn_
Cuts at: 1353 3364 1353
Size: 2011 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2305 2343 4021 4395 4541 387
Size: 1064 138 716 38 1678 374 146 1280
Fragments arranged by size:
1678 1280 1064 716 374 146 138 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2305 2621 3497 4094 4881 5149 1239
Size: 393 673 316 876 597 787 268 1524
Fragments arranged by size:
1524 876 787 673 597 393 316 268
EcoO109I rG'GnC_Cy
Cuts at: 2228 2239 2264 2273 2228
Size: 11 25 9 5389
Fragments arranged by size:
5389 25 11 9
EcoRI G'AATT_C
Cuts at: 2743 3121 2743
Size: 378 5056
FspI TGC'GCA
Cuts at: 3940 3940
Size: 5434
HaeII r_GCGC'y
Cuts at: 1678 2328 2335 4581 1678
Size: 650 7 2246 2531
Fragments arranged by size:
2531 2246 650 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4075 4107 4149 4181 1373
Size: 32 135 32 2503 32 42 32 2626
Fragments arranged by size:
2626 2503 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2778 3024 669
Size: 988 281 840 246 3079
Fragments arranged by size:
3079 988 840 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5434
HpaI GTT'AAC
Cuts at: 3024 3024
Size: 5434
KpnI G_GTAC'C
Cuts at: 2759 2759
Size: 5434
MfeI C'AATT_G
Cuts at: 3033 3033
Size: 5434
MluI A'CGCG_T
Cuts at: 5310 5310
Size: 5434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2730 4548 4732 1305
Size: 1425 1818 184 2007
Fragments arranged by size:
2007 1818 1425 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3612
Fragments arranged by size:
3612 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3392 3751 3910 518
Size: 648 2226 359 159 2042
Fragments arranged by size:
2226 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2270 2850 3527 4675 4920 1606
Size: 664 580 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 580 245
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 5434
NcoI C'CATG_G
Cuts at: 513 1065 2125 2589 513
Size: 552 1060 464 3358
Fragments arranged by size:
3358 1060 552 464
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5434
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 5434
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5434
NotI GC'GGCC_GC
Cuts at: 2881 2881
Size: 5434
NspI r_CATG'y
Cuts at: 1132 1229 2792 4337 1132
Size: 97 1563 1545 2229
Fragments arranged by size:
2229 1563 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5434
PciI A'CATG_T
Cuts at: 4333 4333
Size: 5434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5434
PfoI T'CCnGG_A
Cuts at: 2188 2188
Size: 5434
PmeI GTTT'AAAC
Cuts at: 2738 2738
Size: 5434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 5016 5048 3503
Size: 32 1481 32 3889
Fragments arranged by size:
3889 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2228 2239 2264 2228
Size: 11 25 5398
Fragments arranged by size:
5398 25 11
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5434
PsiI TTA'TAA
Cuts at: 3004 3004
Size: 5434
PspOMI G'GGCC_C
Cuts at: 2273 2273
Size: 5434
PspXI vC'TCGA_Gb
Cuts at: 2232 2232
Size: 5434
PstI C_TGCA'G
Cuts at: 830 2499 2786 830
Size: 1669 287 3478
Fragments arranged by size:
3478 1669 287
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5434
SacII CC_GC'GG
Cuts at: 2271 2271
Size: 5434
SalI G'TCGA_C
Cuts at: 1936 2776 1936
Size: 840 4594
SanDI GG'GwC_CC
Cuts at: 2239 2264 2239
Size: 25 5409
SbfI CC_TGCA'GG
Cuts at: 2786 2786
Size: 5434
ScaI AGT'ACT
Cuts at: 3682 5394 3682
Size: 1712 3722
SfcI C'TryA_G
Cuts at: 826 1045 2495 2782 3917 4598 4789 826
Size: 219 1450 287 1135 681 191 1471
Fragments arranged by size:
1471 1450 1135 681 287 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2154 2163 2281 2154
Size: 9 118 5307
Fragments arranged by size:
5307 118 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5434
SmaI CCC'GGG
Cuts at: 1872 2091 2182 2245 2761 1872
Size: 219 91 63 516 4545
Fragments arranged by size:
4545 516 219 91 63
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2232 3538 4439 4701 820
Size: 197 926 289 1306 901 262 1553
Fragments arranged by size:
1553 1306 926 901 289 262 197
SnaBI TAC'GTA
Cuts at: 493 3225 493
Size: 2732 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5434
SphI G_CATG'C
Cuts at: 1132 2792 1132
Size: 1660 3774
SspI AAT'ATT
Cuts at: 5 52 3358 5
Size: 47 3306 2081
Fragments arranged by size:
3306 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5434
StyI C'CwwG_G
Cuts at: 513 1065 2125 2589 2873 513
Size: 552 1060 464 284 3074
Fragments arranged by size:
3074 1060 552 464 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2611 2621 2627 3461 3478 3631 3816
Size: 1255 1031 10 6 834 17 153 185
Cuts at: 3816 4261 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1031 834 445 185 153 17
10 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2700 3680 5392
Size: 274 80 33 51 1443 723 980 1712
Cuts at: 5392 96
Size: 138
Fragments arranged by size:
1712 1443 980 723 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2004 2036 2603 2635 2682 2714 5394 5426 2004
Size: 32 567 32 47 32 2680 32 2012
Fragments arranged by size:
2680 2012 567 47 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2417 1316
Size: 1101 4333
XbaI T'CTAG_A
Cuts at: 2770 2770
Size: 5434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2596 2596
Size: 5434
XhoI C'TCGA_G
Cuts at: 1943 2232 1943
Size: 289 5145
XmaI C'CCGG_G
Cuts at: 1870 2089 2180 2243 2759 1870
Size: 219 91 63 516 4545
Fragments arranged by size:
4545 516 219 91 63
XmnI GAAnn'nnTTC
Cuts at: 3563 3563
Size: 5434
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3242 276
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PsrI PvuII RsrII SacI SapI SexAI SfoI SrfI
SwaI