Restriction map for
pFN21AE1218
(Circular) (Six-base) MAPSORT of: pFN21AE1218.seq Check: 627 from: 1 to: 5344
pFN21AE1218 5344 bp
With 182 enzymes: SgfI *
November 11, 2016 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3154 278
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2687 1937
Size: 750 4594
Acc65I G'GTAC_C
Cuts at: 2665 2665
Size: 5344
AclI AA'CG_TT
Cuts at: 1563 3471 3844 1563
Size: 1908 373 3063
Fragments arranged by size:
3063 1908 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3407 4791 5059 1632
Size: 1775 1384 268 1917
Fragments arranged by size:
1917 1775 1384 268
AfeI AGC'GCT
Cuts at: 2455 2455
Size: 5344
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5147
AflIII A'CryG_T
Cuts at: 1203 4243 5220 1203
Size: 3040 977 1327
Fragments arranged by size:
3040 1327 977
AgeI A'CCGG_T
Cuts at: 2300 4175 2300
Size: 1875 3469
AhdI GACnn_n'nnGTC
Cuts at: 4073 4073
Size: 5344
AleI CACnn'nnGTG
Cuts at: 1166 2282 1166
Size: 1116 4228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2300 1184
Size: 1116 4228
AlwNI CAG_nnn'CTG
Cuts at: 3087 4659 3087
Size: 1572 3772
ApaLI G'TGCA_C
Cuts at: 3401 4557 3401
Size: 1156 4188
ApoI r'AATT_y
Cuts at: 1493 2653 2879 3031 5140 1493
Size: 1160 226 152 2109 1697
Fragments arranged by size:
2109 1697 1160 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3898 160
Size: 872 2866 1606
Fragments arranged by size:
2866 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
AvaI C'yCGr_G
Cuts at: 1870 1943 2669 1870
Size: 73 726 4545
Fragments arranged by size:
4545 726 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2224 2257 2224
Size: 33 5311
BamHI G'GATC_C
Cuts at: 2674 3041 2674
Size: 367 4977
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2297 2629 2665 4120 618
Size: 325 217 627 510 332 36 1455 1842
Fragments arranged by size:
1842 1455 627 510 332 325 217 36
BanII G_rGCy'C
Cuts at: 1391 2133 2525 2663 1391
Size: 742 392 138 4072
Fragments arranged by size:
4072 742 392 138
BbeI G_GCGC'C
Cuts at: 2633 2633
Size: 5344
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4373
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3535 3569 1903
Size: 34 1598 34 3678
Fragments arranged by size:
3678 1598 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3236 4446 3236
Size: 1210 4134
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5344
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2084 3955 136
Size: 107 122 71 631 605 412 1871 1525
Fragments arranged by size:
1871 1525 631 605 412 122 107 71
BglII A'GATC_T
Cuts at: 5339 5339
Size: 5344
BlpI GC'TnA_GC
Cuts at: 2598 2761 2598
Size: 163 5181
Bme1580I G_kGCm'C
Cuts at: 1790 2279 3405 4561 1790
Size: 489 1126 1156 2573
Fragments arranged by size:
2573 1156 1126 489
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3075 4033 442
Size: 416 53 83 399 24 1658 958 1753
Fragments arranged by size:
1753 1658 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4004 1239
Size: 2765 2579
Bpu10I CC'TnA_GC
Cuts at: 2574 2574
Size: 5344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 3469 4334 4632 2240
Size: 1229 865 298 2952
Fragments arranged by size:
2952 1229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2258 4007 882
Size: 617 759 1749 2219
Fragments arranged by size:
2219 1749 759 617
BsaAI yAC'GTr
Cuts at: 493 1204 3135 493
Size: 711 1931 2702
Fragments arranged by size:
2702 1931 711
BsaBI GATnn'nnATC
Cuts at: 5338 5338
Size: 5344
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2108 2630 3151 3533
Size: 53 83 186 936 575 522 521 382
Cuts at: 3533 4168 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 575 522 521 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2111 2300 3777 4175 4449 4596 5007 2111
Size: 189 1477 398 274 147 411 2448
Fragments arranged by size:
2448 1477 411 398 274 189 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5119
Fragments arranged by size:
5119 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5344
BseYI C'CCAG_C
Cuts at: 2087 4547 5043 2087
Size: 2460 496 2388
Fragments arranged by size:
2460 2388 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4771
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2490 2794 3555 4583 1540
Size: 275 186 489 304 761 1028 2301
Fragments arranged by size:
2301 1028 761 489 304 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 3405 3490 4561 2133
Size: 1272 85 1071 2916
Fragments arranged by size:
2916 1272 1071 85
BsmI GAATG_Cn'
Cuts at: 2855 2948 2855
Size: 93 5251
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2279 2525 2663 3405 3490 4561
Size: 399 343 146 246 138 742 85 1071
Cuts at: 4561 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 343 246 146 138
85
BspEI T'CCGG_A
Cuts at: 5007 5007
Size: 5344
BspHI T'CATG_A
Cuts at: 3233 4963 3233
Size: 1730 3614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2699 844
Size: 1855 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2027 3231 1394
Size: 429 204 1204 3507
Fragments arranged by size:
3507 1204 429 204
BsrDI GCAATG_nn'
Cuts at: 65 1248 3839 4013 65
Size: 1183 2591 174 1396
Fragments arranged by size:
2591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2210 2300 2485 3988 4175 1700
Size: 110 400 90 185 1503 187 2869
Fragments arranged by size:
2869 1503 400 187 185 110 90
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5344
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5344
BssSI C'ACGA_G
Cuts at: 3404 4416 3404
Size: 1012 4332
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5344
BstBI TT'CG_AA
Cuts at: 4148 4148
Size: 5344
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5344
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2512 2146
Size: 366 4978
BstYI r'GATC_y
Cuts at: 1972 2674 3041 3426 3443 4895 4981 4993 5339
Size: 702 367 385 17 1452 86 12 346
Cuts at: 5339 1972
Size: 1977
Fragments arranged by size:
1977 1452 702 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2490 513
Size: 552 1425 3367
Fragments arranged by size:
3367 1425 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4605
Fragments arranged by size:
4605 683 56
BtsI GCAGTG_nn'
Cuts at: 2860 3654 3674 2860
Size: 794 20 4530
Fragments arranged by size:
4530 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5344
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5309
DraI TTT'AAA
Cuts at: 2320 2648 2320
Size: 328 5016
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4351 809
Size: 507 3035 1802
Fragments arranged by size:
3035 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2148 2487
Size: 54 1425 50 275 18 246 72 339
Cuts at: 2487 2493 2791 3680 5309 8
Size: 6 298 889 1629 43
Fragments arranged by size:
1629 1425 889 339 298 275 246 72
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2487 2791 1537
Size: 275 675 304 4090
Fragments arranged by size:
4090 675 304 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2444 3274 1353
Size: 1091 830 3423
Fragments arranged by size:
3423 1091 830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2391 3931 4305 4451 387
Size: 1064 138 802 1540 374 146 1280
Fragments arranged by size:
1540 1280 1064 802 374 146 138
EcoICRI GAG'CTC
Cuts at: 2131 2131
Size: 5344
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3407 4004 4791 5059 1239
Size: 393 1775 597 787 268 1524
Fragments arranged by size:
1775 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2056 2056
Size: 5344
EcoRI G'AATT_C
Cuts at: 2653 3031 2653
Size: 378 4966
FspI TGC'GCA
Cuts at: 3850 3850
Size: 5344
HaeII r_GCGC'y
Cuts at: 1678 2457 2633 4491 1678
Size: 779 176 1858 2531
Fragments arranged by size:
2531 1858 779 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3985 4017 4059 4091 1373
Size: 32 135 32 2413 32 42 32 2626
Fragments arranged by size:
2626 2413 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2688 2934 669
Size: 988 281 750 246 3079
Fragments arranged by size:
3079 988 750 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5344
HpaI GTT'AAC
Cuts at: 2934 2934
Size: 5344
KasI G'GCGC_C
Cuts at: 2629 2629
Size: 5344
KpnI G_GTAC'C
Cuts at: 2669 2669
Size: 5344
MfeI C'AATT_G
Cuts at: 2943 2943
Size: 5344
MluI A'CGCG_T
Cuts at: 5220 5220
Size: 5344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4458 4642 1305
Size: 3153 184 2007
Fragments arranged by size:
3153 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3522
Fragments arranged by size:
3522 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2282 3302 3661 3820 518
Size: 648 1116 1020 359 159 2042
Fragments arranged by size:
2042 1116 1020 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2033 2492 2760 3437 4585 4830 1606
Size: 427 459 268 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 459 427 268 245
NaeI GCC'GGC
Cuts at: 2212 2487 2212
Size: 275 5069
NarI GG'CG_CC
Cuts at: 2630 2630
Size: 5344
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4792
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5344
NgoMIV G'CCGG_C
Cuts at: 2210 2485 2210
Size: 275 5069
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5344
NotI GC'GGCC_GC
Cuts at: 2791 2791
Size: 5344
NspI r_CATG'y
Cuts at: 1132 1229 2702 4247 1132
Size: 97 1473 1545 2229
Fragments arranged by size:
2229 1545 1473 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5344
PciI A'CATG_T
Cuts at: 4243 4243
Size: 5344
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5344
PmeI GTTT'AAAC
Cuts at: 2648 2648
Size: 5344
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 4926 4958 3413
Size: 32 1481 32 3799
Fragments arranged by size:
3799 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5344
PsiI TTA'TAA
Cuts at: 2914 2914
Size: 5344
PstI C_TGCA'G
Cuts at: 830 2696 830
Size: 1866 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5344
SacI G_AGCT'C
Cuts at: 2133 2133
Size: 5344
SacII CC_GC'GG
Cuts at: 2493 2493
Size: 5344
SalI G'TCGA_C
Cuts at: 1936 2686 1936
Size: 750 4594
SapI GCTCTTCn'nnn_
Cuts at: 2444 2444
Size: 5344
SbfI CC_TGCA'GG
Cuts at: 2696 2696
Size: 5344
ScaI AGT'ACT
Cuts at: 3592 5304 3592
Size: 1712 3632
SfcI C'TryA_G
Cuts at: 826 1045 2692 3827 4508 4699 826
Size: 219 1647 1135 681 191 1471
Fragments arranged by size:
1647 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2631 2631
Size: 5344
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
SgrAI Cr'CCGG_yG
Cuts at: 1700 2300 1700
Size: 600 4744
SmaI CCC'GGG
Cuts at: 1872 2671 1872
Size: 799 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 3448 4349 4611 820
Size: 197 926 312 1193 901 262 1553
Fragments arranged by size:
1553 1193 926 901 312 262 197
SnaBI TAC'GTA
Cuts at: 493 3135 493
Size: 2642 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5344
SphI G_CATG'C
Cuts at: 1132 2702 1132
Size: 1570 3774
SspI AAT'ATT
Cuts at: 5 52 3268 5
Size: 47 3216 2081
Fragments arranged by size:
3216 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5344
StyI C'CwwG_G
Cuts at: 513 1065 2139 2358 2535 2589 2604 2783 513
Size: 552 1074 219 177 54 15 179 3074
Fragments arranged by size:
3074 1074 552 219 179 177 54 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3371 3388 3541 3726 4171 325
Size: 1255 1791 17 153 185 445 1498
Fragments arranged by size:
1791 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3590 5302 96
Size: 274 80 33 51 1443 1613 1712 138
Fragments arranged by size:
1712 1613 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5304 5336 5304
Size: 32 5312
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5344
XbaI T'CTAG_A
Cuts at: 2680 2680
Size: 5344
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5344
XmaI C'CCGG_G
Cuts at: 1870 2669 1870
Size: 799 4545
XmnI GAAnn'nnTTC
Cuts at: 3473 3473
Size: 5344
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3152 276
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SanDI SexAI SfiI
SrfI SwaI XcmI