Restriction map for
pFN21AE1216
(Circular) (Six-base) MAPSORT of: pFN21AE1216.seq Check: 7961 from: 1 to: 5242
pFN21AE1216 5242 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3052 278
Size: 53 83 186 936 1516 2468
Fragments arranged by size:
2468 1516 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2585 1937
Size: 648 4594
Acc65I G'GTAC_C
Cuts at: 2563 2563
Size: 5242
AclI AA'CG_TT
Cuts at: 1563 3369 3742 1563
Size: 1806 373 3063
Fragments arranged by size:
3063 1806 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2498 3305 4689 4957 1632
Size: 866 807 1384 268 1917
Fragments arranged by size:
1917 1384 866 807 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5045
AflIII A'CryG_T
Cuts at: 1203 4141 5118 1203
Size: 2938 977 1327
Fragments arranged by size:
2938 1327 977
AgeI A'CCGG_T
Cuts at: 4073 4073
Size: 5242
AhdI GACnn_n'nnGTC
Cuts at: 3971 3971
Size: 5242
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5242
AlwNI CAG_nnn'CTG
Cuts at: 2204 2985 4557 2204
Size: 781 1572 2889
Fragments arranged by size:
2889 1572 781
ApaI G_GGCC'C
Cuts at: 2225 2225
Size: 5242
ApaLI G'TGCA_C
Cuts at: 3299 4455 3299
Size: 1156 4086
ApoI r'AATT_y
Cuts at: 1493 2551 2777 2929 5038 1493
Size: 1058 226 152 2109 1697
Fragments arranged by size:
2109 1697 1058 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3796 160
Size: 872 2764 1606
Fragments arranged by size:
2764 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5242
AvaI C'yCGr_G
Cuts at: 1870 1943 2079 2567 1870
Size: 73 136 488 4545
Fragments arranged by size:
4545 488 136 73
BamHI G'GATC_C
Cuts at: 2572 2939 2572
Size: 367 4875
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2284 2563 4018 618
Size: 325 217 627 282 215 279 1455 1842
Fragments arranged by size:
1842 1455 627 325 282 279 217 215
BanII G_rGCy'C
Cuts at: 1391 2091 2225 2561 1391
Size: 700 134 336 4072
Fragments arranged by size:
4072 700 336 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2088 2486 928
Size: 971 189 398 3684
Fragments arranged by size:
3684 971 398 189
BbvCI CC'TCA_GC
Cuts at: 2165 2165
Size: 5242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3433 3467 1903
Size: 34 50 34 1412 34 3678
Fragments arranged by size:
3678 1412 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2042 3134 4344 2042
Size: 1092 1210 2940
Fragments arranged by size:
2940 1210 1092
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5242
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2229 3853 136
Size: 107 122 71 631 605 557 1624 1525
Fragments arranged by size:
1624 1525 631 605 557 122 107 71
BglII A'GATC_T
Cuts at: 5237 5237
Size: 5242
BlpI GC'TnA_GC
Cuts at: 2659 2659
Size: 5242
Bme1580I G_kGCm'C
Cuts at: 1790 2225 3303 4459 1790
Size: 435 1078 1156 2573
Fragments arranged by size:
2573 1156 1078 435
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5242
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2227 2973 3931
Size: 416 53 83 399 24 810 746 958
Cuts at: 3931 442
Size: 1753
Fragments arranged by size:
1753 958 810 746 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 3902 1239
Size: 917 1746 2579
Fragments arranged by size:
2579 1746 917
Bpu10I CC'TnA_GC
Cuts at: 2165 2432 2165
Size: 267 4975
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2018 3367 4232 4530 2018
Size: 1349 865 298 2730
Fragments arranged by size:
2730 1349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3905 882
Size: 617 2406 2219
Fragments arranged by size:
2406 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3033 493
Size: 711 1829 2702
Fragments arranged by size:
2702 1829 711
BsaBI GATnn'nnATC
Cuts at: 5236 5236
Size: 5242
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3049 3431 4066 275
Size: 53 83 186 936 1516 382 635 1451
Fragments arranged by size:
1516 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2091 3675 4073 4347 4494 4905 2091
Size: 1584 398 274 147 411 2428
Fragments arranged by size:
2428 1584 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5017
Fragments arranged by size:
5017 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 1186
Size: 967 4275
BseYI C'CCAG_C
Cuts at: 2508 4445 4941 2508
Size: 1937 496 2809
Fragments arranged by size:
2809 1937 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4669
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2692 3453 4481 1540
Size: 275 186 691 761 1028 2301
Fragments arranged by size:
2301 1028 761 691 275 186
BsiHKAI G_wGCw'C
Cuts at: 3303 3388 4459 3303
Size: 85 1071 4086
Fragments arranged by size:
4086 1071 85
BsmI GAATG_Cn'
Cuts at: 2753 2846 2753
Size: 93 5149
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2225 2561 3303 3388 4459 1391
Size: 399 301 134 336 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 336 301 134 85
BspEI T'CCGG_A
Cuts at: 4905 4905
Size: 5242
BspHI T'CATG_A
Cuts at: 3131 4861 3131
Size: 1730 3512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2597 844
Size: 1753 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2400 3129 1394
Size: 429 577 729 3507
Fragments arranged by size:
3507 729 577 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2310 3737 3911 65
Size: 1183 1062 1427 174 1396
Fragments arranged by size:
1427 1396 1183 1062 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3886 4073 1700
Size: 110 2076 187 2869
Fragments arranged by size:
2869 2076 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5242
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5242
BssSI C'ACGA_G
Cuts at: 2010 3302 4314 2010
Size: 1292 1012 2938
Fragments arranged by size:
2938 1292 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5242
BstBI TT'CG_AA
Cuts at: 4046 4046
Size: 5242
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5242
BstYI r'GATC_y
Cuts at: 1972 2457 2572 2939 3324 3341 4793 4879 4891
Size: 485 115 367 385 17 1452 86 12
Cuts at: 4891 5237 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 485 385 367 346 115 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2106 2233 513
Size: 552 1041 127 3522
Fragments arranged by size:
3522 1041 552 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2505 487
Size: 683 56 1279 3224
Fragments arranged by size:
3224 1279 683 56
BtsI GCAGTG_nn'
Cuts at: 2758 3552 3572 2758
Size: 794 20 4428
Fragments arranged by size:
4428 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5242
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5207
DraI TTT'AAA
Cuts at: 2546 2546
Size: 5242
DraIII CAC_nnn'GTG
Cuts at: 2284 2284
Size: 5242
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4249 809
Size: 507 2933 1802
Fragments arranged by size:
2933 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2689 3578 5207
Size: 54 1425 50 275 18 859 889 1629
Cuts at: 5207 8
Size: 43
Fragments arranged by size:
1629 1425 889 859 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2689 1537
Size: 275 877 4090
Fragments arranged by size:
4090 877 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3172 1353
Size: 1819 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 3829 4203 4349 387
Size: 1064 138 522 1718 374 146 1280
Fragments arranged by size:
1718 1280 1064 522 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2498 3305 3902 4689 4957 1239
Size: 393 524 342 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 524 393 342 268
EcoO109I rG'GnC_Cy
Cuts at: 2221 2222 2374 2221
Size: 1 152 5089
Fragments arranged by size:
5089 152 1
EcoRI G'AATT_C
Cuts at: 2551 2929 2551
Size: 378 4864
FspI TGC'GCA
Cuts at: 3748 3748
Size: 5242
HaeII r_GCGC'y
Cuts at: 1678 4389 1678
Size: 2711 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3883 3915 3957 3989 1373
Size: 32 135 32 2311 32 42 32 2626
Fragments arranged by size:
2626 2311 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2586 2832 669
Size: 988 281 648 246 3079
Fragments arranged by size:
3079 988 648 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5242
HpaI GTT'AAC
Cuts at: 2832 2832
Size: 5242
KpnI G_GTAC'C
Cuts at: 2567 2567
Size: 5242
MfeI C'AATT_G
Cuts at: 2841 2841
Size: 5242
MluI A'CGCG_T
Cuts at: 5118 5118
Size: 5242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2369 4356 4540 1305
Size: 1064 1987 184 2007
Fragments arranged by size:
2007 1987 1064 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3420
Fragments arranged by size:
3420 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3200 3559 3718 518
Size: 648 2034 359 159 2042
Fragments arranged by size:
2042 2034 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2476 2658 3335 4483 4728 1606
Size: 870 182 677 1148 245 2120
Fragments arranged by size:
2120 1148 870 677 245 182
NcoI C'CATG_G
Cuts at: 513 1065 2233 513
Size: 552 1168 3522
Fragments arranged by size:
3522 1168 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5242
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5242
NotI GC'GGCC_GC
Cuts at: 2689 2689
Size: 5242
NspI r_CATG'y
Cuts at: 1132 1229 2193 2600 4145 1132
Size: 97 964 407 1545 2229
Fragments arranged by size:
2229 1545 964 407 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5242
PciI A'CATG_T
Cuts at: 4141 4141
Size: 5242
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5242
PmeI GTTT'AAAC
Cuts at: 2546 2546
Size: 5242
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3343 4824 4856 3311
Size: 32 1481 32 3697
Fragments arranged by size:
3697 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2374 2374
Size: 5242
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5242
PsiI TTA'TAA
Cuts at: 2812 2812
Size: 5242
PspOMI G'GGCC_C
Cuts at: 2221 2221
Size: 5242
PstI C_TGCA'G
Cuts at: 830 2230 2594 830
Size: 1400 364 3478
Fragments arranged by size:
3478 1400 364
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5242
SalI G'TCGA_C
Cuts at: 1936 2584 1936
Size: 648 4594
SbfI CC_TGCA'GG
Cuts at: 2230 2594 2230
Size: 364 4878
ScaI AGT'ACT
Cuts at: 3490 5202 3490
Size: 1712 3530
SexAI A'CCwGG_T
Cuts at: 2280 2280
Size: 5242
SfcI C'TryA_G
Cuts at: 826 1045 2226 2590 3725 4406 4597 826
Size: 219 1181 364 1135 681 191 1471
Fragments arranged by size:
1471 1181 1135 681 364 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2229 2229
Size: 5242
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5242
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5242
SmaI CCC'GGG
Cuts at: 1872 2081 2569 1872
Size: 209 488 4545
Fragments arranged by size:
4545 488 209
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2033 3346 4247 4509 820
Size: 197 926 90 1313 901 262 1553
Fragments arranged by size:
1553 1313 926 901 262 197 90
SnaBI TAC'GTA
Cuts at: 493 3033 493
Size: 2540 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5242
SphI G_CATG'C
Cuts at: 1132 2193 2600 1132
Size: 1061 407 3774
Fragments arranged by size:
3774 1061 407
SspI AAT'ATT
Cuts at: 5 52 3166 5
Size: 47 3114 2081
Fragments arranged by size:
3114 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5242
StyI C'CwwG_G
Cuts at: 513 1065 2233 2493 2536 2681 513
Size: 552 1168 260 43 145 3074
Fragments arranged by size:
3074 1168 552 260 145 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3269 3286 3439 3624 4069 325
Size: 1255 1689 17 153 185 445 1498
Fragments arranged by size:
1689 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3488 5200 96
Size: 274 80 33 51 1443 1511 1712 138
Fragments arranged by size:
1712 1511 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5202 5234 5202
Size: 32 5210
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5242
XbaI T'CTAG_A
Cuts at: 2578 2578
Size: 5242
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5242
XmaI C'CCGG_G
Cuts at: 1870 2079 2567 1870
Size: 209 488 4545
Fragments arranged by size:
4545 488 209
XmnI GAAnn'nnTTC
Cuts at: 3371 3371
Size: 5242
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3050 276
Size: 53 83 186 936 1516 2468
Fragments arranged by size:
2468 1516 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfoI SrfI SwaI
XcmI