Restriction map for
pFN21AE1204
(Circular) (Six-base) MAPSORT of: pFN21AE1204.seq Check: 1592 from: 1 to: 5050
pFN21AE1204 5050 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2860 278
Size: 53 83 186 936 1324 2468
Fragments arranged by size:
2468 1324 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2393 1937
Size: 456 4594
Acc65I G'GTAC_C
Cuts at: 2371 2371
Size: 5050
AclI AA'CG_TT
Cuts at: 1563 3177 3550 1563
Size: 1614 373 3063
Fragments arranged by size:
3063 1614 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3113 4497 4765 1632
Size: 1481 1384 268 1917
Fragments arranged by size:
1917 1481 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4853
AflIII A'CryG_T
Cuts at: 1203 3949 4926 1203
Size: 2746 977 1327
Fragments arranged by size:
2746 1327 977
AgeI A'CCGG_T
Cuts at: 3881 3881
Size: 5050
AhdI GACnn_n'nnGTC
Cuts at: 3779 3779
Size: 5050
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5050
AlwNI CAG_nnn'CTG
Cuts at: 2793 4365 2793
Size: 1572 3478
ApaI G_GGCC'C
Cuts at: 2068 2068
Size: 5050
ApaLI G'TGCA_C
Cuts at: 3107 4263 3107
Size: 1156 3894
ApoI r'AATT_y
Cuts at: 1493 2359 2585 2737 4846 1493
Size: 866 226 152 2109 1697
Fragments arranged by size:
2109 1697 866 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3604 160
Size: 872 2572 1606
Fragments arranged by size:
2572 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5050
AvaI C'yCGr_G
Cuts at: 1870 1943 2375 1870
Size: 73 432 4545
Fragments arranged by size:
4545 432 73
BamHI G'GATC_C
Cuts at: 2380 2747 2380
Size: 367 4683
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2371 3826 618
Size: 325 217 627 584 1455 1842
Fragments arranged by size:
1842 1455 627 584 325 217
BanII G_rGCy'C
Cuts at: 1391 2068 2305 2369 1391
Size: 677 237 64 4072
Fragments arranged by size:
4072 677 237 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4079
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3241 3275 1903
Size: 34 1304 34 3678
Fragments arranged by size:
3678 1304 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2285 2942 4152 2285
Size: 657 1210 3183
Fragments arranged by size:
3183 1210 657
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5050
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2183 3661 136
Size: 107 122 71 631 605 511 1478 1525
Fragments arranged by size:
1525 1478 631 605 511 122 107 71
BglII A'GATC_T
Cuts at: 5045 5045
Size: 5050
BlpI GC'TnA_GC
Cuts at: 2467 2467
Size: 5050
Bme1580I G_kGCm'C
Cuts at: 1790 2068 3111 4267 1790
Size: 278 1043 1156 2573
Fragments arranged by size:
2573 1156 1043 278
BmgBI CAC'GTC
Cuts at: 1318 2080 1318
Size: 762 4288
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2307 2781 3739
Size: 416 53 83 399 24 890 474 958
Cuts at: 3739 442
Size: 1753
Fragments arranged by size:
1753 958 890 474 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2031 2246 2337 3710 1239
Size: 792 215 91 1373 2579
Fragments arranged by size:
2579 1373 792 215 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3175 4040 4338 3175
Size: 865 298 3887
Fragments arranged by size:
3887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2029 3713 882
Size: 617 530 1684 2219
Fragments arranged by size:
2219 1684 617 530
BsaAI yAC'GTr
Cuts at: 493 1204 2841 493
Size: 711 1637 2702
Fragments arranged by size:
2702 1637 711
BsaBI GATnn'nnATC
Cuts at: 2223 5044 2223
Size: 2821 2229
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2857 3239 3874 275
Size: 53 83 186 936 1324 382 635 1451
Fragments arranged by size:
1451 1324 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2057 3483 3881 4155 4302 4713 2057
Size: 1426 398 274 147 411 2394
Fragments arranged by size:
2394 1426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4825
Fragments arranged by size:
4825 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2108 1186
Size: 922 4128
BseYI C'CCAG_C
Cuts at: 2105 4253 4749 2105
Size: 2148 496 2406
Fragments arranged by size:
2406 2148 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4477
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2500 3261 4289 1540
Size: 275 186 499 761 1028 2301
Fragments arranged by size:
2301 1028 761 499 275 186
BsiHKAI G_wGCw'C
Cuts at: 2158 3111 3196 4267 2158
Size: 953 85 1071 2941
Fragments arranged by size:
2941 1071 953 85
BsmI GAATG_Cn'
Cuts at: 2561 2654 2561
Size: 93 4957
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2158 2305 2369 3111 3196 4267
Size: 399 278 90 147 64 742 85 1071
Cuts at: 4267 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 278 147 90 85
64
BspEI T'CCGG_A
Cuts at: 2057 4713 2057
Size: 2656 2394
BspHI T'CATG_A
Cuts at: 2939 4669 2939
Size: 1730 3320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2405 844
Size: 1561 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2937 1394
Size: 429 1114 3507
Fragments arranged by size:
3507 1114 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3545 3719 65
Size: 1183 2297 174 1396
Fragments arranged by size:
2297 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3694 3881 1700
Size: 110 1884 187 2869
Fragments arranged by size:
2869 1884 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5050
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5050
BssSI C'ACGA_G
Cuts at: 3110 4122 3110
Size: 1012 4038
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5050
BstBI TT'CG_AA
Cuts at: 3854 3854
Size: 5050
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5050
BstXI CCAn_nnnn'nTGG
Cuts at: 2275 2275
Size: 5050
BstYI r'GATC_y
Cuts at: 1972 2380 2747 3132 3149 4601 4687 4699 5045
Size: 408 367 385 17 1452 86 12 346
Cuts at: 5045 1972
Size: 1977
Fragments arranged by size:
1977 1452 408 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2019 513
Size: 552 939 15 3544
Fragments arranged by size:
3544 939 552 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4311
Fragments arranged by size:
4311 683 56
BtsI GCAGTG_nn'
Cuts at: 2566 3360 3380 2566
Size: 794 20 4236
Fragments arranged by size:
4236 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5050
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5015
DraI TTT'AAA
Cuts at: 2354 2354
Size: 5050
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4057 809
Size: 507 2741 1802
Fragments arranged by size:
2741 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2497 3386 5015
Size: 54 1425 50 275 18 667 889 1629
Cuts at: 5015 8
Size: 43
Fragments arranged by size:
1629 1425 889 667 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2497 1537
Size: 275 685 4090
Fragments arranged by size:
4090 685 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 2980 1353
Size: 725 902 3423
Fragments arranged by size:
3423 902 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3637 4011 4157 387
Size: 1064 138 2048 374 146 1280
Fragments arranged by size:
2048 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2246 2337 3113 3710 4497 4765
Size: 393 399 215 91 776 597 787 268
Cuts at: 4765 1239
Size: 1524
Fragments arranged by size:
1524 787 776 597 399 393 268 215
91
EcoO109I rG'GnC_Cy
Cuts at: 2257 2257
Size: 5050
EcoRI G'AATT_C
Cuts at: 2359 2737 2359
Size: 378 4672
FspI TGC'GCA
Cuts at: 3556 3556
Size: 5050
HaeII r_GCGC'y
Cuts at: 1678 2018 2126 4197 1678
Size: 340 108 2071 2531
Fragments arranged by size:
2531 2071 340 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 3691 3723 3765
Size: 32 135 32 497 32 1590 32 42
Cuts at: 3765 3797 1373
Size: 32 2626
Fragments arranged by size:
2626 1590 497 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2394 2640 669
Size: 988 281 456 246 3079
Fragments arranged by size:
3079 988 456 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5050
HpaI GTT'AAC
Cuts at: 2640 2640
Size: 5050
KpnI G_GTAC'C
Cuts at: 2375 2375
Size: 5050
MfeI C'AATT_G
Cuts at: 2649 2649
Size: 5050
MluI A'CGCG_T
Cuts at: 4926 4926
Size: 5050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 2316 4164 4348 1305
Size: 822 189 1848 184 2007
Fragments arranged by size:
2007 1848 822 189 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3228
Fragments arranged by size:
3228 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 3008 3367 3526 518
Size: 648 969 873 359 159 2042
Fragments arranged by size:
2042 969 873 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2105 2174 2275 2466 3143 4291 4536 1606
Size: 499 69 101 191 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 499 245 191 101 69
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3559
Fragments arranged by size:
3559 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5050
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5050
NotI GC'GGCC_GC
Cuts at: 2497 2497
Size: 5050
NruI TCG'CGA
Cuts at: 2091 2091
Size: 5050
NspI r_CATG'y
Cuts at: 1132 1229 2408 3953 1132
Size: 97 1179 1545 2229
Fragments arranged by size:
2229 1545 1179 97
PasI CC'CwG_GG
Cuts at: 1348 2117 1348
Size: 769 4281
PciI A'CATG_T
Cuts at: 3949 3949
Size: 5050
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5050
PmeI GTTT'AAAC
Cuts at: 2354 2354
Size: 5050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3119 3151 4632 4664 3119
Size: 32 1481 32 3505
Fragments arranged by size:
3505 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5050
PsiI TTA'TAA
Cuts at: 2620 2620
Size: 5050
PspOMI G'GGCC_C
Cuts at: 2064 2064
Size: 5050
PstI C_TGCA'G
Cuts at: 830 2163 2402 830
Size: 1333 239 3478
Fragments arranged by size:
3478 1333 239
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5050
PvuII CAG'CTG
Cuts at: 2105 2174 2275 2105
Size: 69 101 4880
Fragments arranged by size:
4880 101 69
SalI G'TCGA_C
Cuts at: 1936 2392 1936
Size: 456 4594
SapI GCTCTTCn'nnn_
Cuts at: 2078 2078
Size: 5050
SbfI CC_TGCA'GG
Cuts at: 2402 2402
Size: 5050
ScaI AGT'ACT
Cuts at: 3298 5010 3298
Size: 1712 3338
SfcI C'TryA_G
Cuts at: 826 1045 2159 2323 2398 3533 4214 4405 826
Size: 219 1114 164 75 1135 681 191 1471
Fragments arranged by size:
1471 1135 1114 681 219 191 164 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5050
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5050
SmaI CCC'GGG
Cuts at: 1872 2377 1872
Size: 505 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3154 4055 4317 820
Size: 197 926 1211 901 262 1553
Fragments arranged by size:
1553 1211 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2841 493
Size: 2348 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5050
SphI G_CATG'C
Cuts at: 1132 2408 1132
Size: 1276 3774
SspI AAT'ATT
Cuts at: 5 52 2974 5
Size: 47 2922 2081
Fragments arranged by size:
2922 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5050
StyI C'CwwG_G
Cuts at: 513 1065 2004 2489 513
Size: 552 939 485 3074
Fragments arranged by size:
3074 939 552 485
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3077 3094 3247 3432 3877 325
Size: 1255 1497 17 153 185 445 1498
Fragments arranged by size:
1498 1497 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3296 5008 96
Size: 274 80 33 51 1443 1319 1712 138
Fragments arranged by size:
1712 1443 1319 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5010 5042 5010
Size: 32 5018
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5050
XbaI T'CTAG_A
Cuts at: 2386 2386
Size: 5050
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5050
XmaI C'CCGG_G
Cuts at: 1870 2375 1870
Size: 505 4545
XmnI GAAnn'nnTTC
Cuts at: 3179 3179
Size: 5050
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2858 276
Size: 53 83 186 936 1324 2468
Fragments arranged by size:
2468 1324 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI