Restriction map for
pFN21AE1201
(Circular) (Six-base) MAPSORT of: pFN21AE1201.seq Check: 6432 from: 1 to: 4831
pFN21AE1201 4831 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2641 278
Size: 53 83 186 936 1105 2468
Fragments arranged by size:
2468 1105 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2174 1937
Size: 237 4594
Acc65I G'GTAC_C
Cuts at: 2152 2152
Size: 4831
AclI AA'CG_TT
Cuts at: 1563 2958 3331 1563
Size: 1395 373 3063
Fragments arranged by size:
3063 1395 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2894 4278 4546 1632
Size: 1262 1384 268 1917
Fragments arranged by size:
1917 1384 1262 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4634
AflIII A'CryG_T
Cuts at: 1203 3730 4707 1203
Size: 2527 977 1327
Fragments arranged by size:
2527 1327 977
AgeI A'CCGG_T
Cuts at: 3662 3662
Size: 4831
AhdI GACnn_n'nnGTC
Cuts at: 3560 3560
Size: 4831
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4831
AlwNI CAG_nnn'CTG
Cuts at: 2574 4146 2574
Size: 1572 3259
ApaLI G'TGCA_C
Cuts at: 2888 4044 2888
Size: 1156 3675
ApoI r'AATT_y
Cuts at: 1493 2140 2366 2518 4627 1493
Size: 647 226 152 2109 1697
Fragments arranged by size:
2109 1697 647 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3385 160
Size: 872 2353 1606
Fragments arranged by size:
2353 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4831
AvaI C'yCGr_G
Cuts at: 1870 1943 2156 1870
Size: 73 213 4545
Fragments arranged by size:
4545 213 73
BamHI G'GATC_C
Cuts at: 2161 2528 2161
Size: 367 4464
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2152 3607 618
Size: 325 217 627 365 1455 1842
Fragments arranged by size:
1842 1455 627 365 325 217
BanII G_rGCy'C
Cuts at: 1391 2150 1391
Size: 759 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3860
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3022 3056 1903
Size: 34 1085 34 3678
Fragments arranged by size:
3678 1085 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2723 3933 2723
Size: 1210 3621
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4831
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3442 136
Size: 107 122 71 631 605 1770 1525
Fragments arranged by size:
1770 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4826 4826
Size: 4831
BlpI GC'TnA_GC
Cuts at: 2248 2248
Size: 4831
Bme1580I G_kGCm'C
Cuts at: 1790 2892 4048 1790
Size: 1102 1156 2573
Fragments arranged by size:
2573 1156 1102
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4831
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2562 3520 442
Size: 416 53 83 399 24 1145 958 1753
Fragments arranged by size:
1753 1145 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3491 1239
Size: 2252 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2956 3821 4119 2956
Size: 865 298 3668
Fragments arranged by size:
3668 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3494 882
Size: 617 1995 2219
Fragments arranged by size:
2219 1995 617
BsaAI yAC'GTr
Cuts at: 493 1204 2622 493
Size: 711 1418 2702
Fragments arranged by size:
2702 1418 711
BsaBI GATnn'nnATC
Cuts at: 4825 4825
Size: 4831
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2638 3020 3655 275
Size: 53 83 186 936 1105 382 635 1451
Fragments arranged by size:
1451 1105 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3264 3662 3936 4083 4494 3264
Size: 398 274 147 411 3601
Fragments arranged by size:
3601 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4606
Fragments arranged by size:
4606 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4831
BseYI C'CCAG_C
Cuts at: 4034 4530 4034
Size: 496 4335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4258
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2281 3042 4070 1540
Size: 275 186 280 761 1028 2301
Fragments arranged by size:
2301 1028 761 280 275 186
BsiHKAI G_wGCw'C
Cuts at: 2892 2977 4048 2892
Size: 85 1071 3675
Fragments arranged by size:
3675 1071 85
BsmI GAATG_Cn'
Cuts at: 2342 2435 2342
Size: 93 4738
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2892 2977 4048 1391
Size: 399 360 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 360 85
BspEI T'CCGG_A
Cuts at: 4494 4494
Size: 4831
BspHI T'CATG_A
Cuts at: 2720 4450 2720
Size: 1730 3101
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2186 844
Size: 1342 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2718 1394
Size: 429 895 3507
Fragments arranged by size:
3507 895 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3326 3500 65
Size: 1183 2078 174 1396
Fragments arranged by size:
2078 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3475 3662 1700
Size: 110 1665 187 2869
Fragments arranged by size:
2869 1665 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4831
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4831
BssSI C'ACGA_G
Cuts at: 2050 2891 3903 2050
Size: 841 1012 2978
Fragments arranged by size:
2978 1012 841
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4831
BstBI TT'CG_AA
Cuts at: 3635 3635
Size: 4831
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4831
BstYI r'GATC_y
Cuts at: 1972 2161 2528 2913 2930 4382 4468 4480 4826
Size: 189 367 385 17 1452 86 12 346
Cuts at: 4826 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 189 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4092
Fragments arranged by size:
4092 683 56
BtsI GCAGTG_nn'
Cuts at: 2347 3141 3161 2347
Size: 794 20 4017
Fragments arranged by size:
4017 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4831
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4796
DraI TTT'AAA
Cuts at: 2135 2135
Size: 4831
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3838 809
Size: 507 2522 1802
Fragments arranged by size:
2522 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2278 3167 4796
Size: 54 1425 50 275 18 448 889 1629
Cuts at: 4796 8
Size: 43
Fragments arranged by size:
1629 1425 889 448 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2278 1537
Size: 275 466 4090
Fragments arranged by size:
4090 466 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2761 1353
Size: 1408 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3418 3792 3938 387
Size: 1064 138 1829 374 146 1280
Fragments arranged by size:
1829 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2894 3491 4278 4546 1239
Size: 393 1262 597 787 268 1524
Fragments arranged by size:
1524 1262 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2140 2518 2140
Size: 378 4453
FspI TGC'GCA
Cuts at: 3337 3337
Size: 4831
HaeII r_GCGC'y
Cuts at: 1678 3978 1678
Size: 2300 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3472 3504 3546 3578 1373
Size: 32 135 32 1900 32 42 32 2626
Fragments arranged by size:
2626 1900 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2175 2421 669
Size: 988 281 237 246 3079
Fragments arranged by size:
3079 988 281 246 237
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4831
HpaI GTT'AAC
Cuts at: 2421 2421
Size: 4831
KpnI G_GTAC'C
Cuts at: 2156 2156
Size: 4831
MfeI C'AATT_G
Cuts at: 2430 2430
Size: 4831
MluI A'CGCG_T
Cuts at: 4707 4707
Size: 4831
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3945 4129 1305
Size: 2640 184 2007
Fragments arranged by size:
2640 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3009
Fragments arranged by size:
3009 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 3148 3307 518
Size: 648 1623 359 159 2042
Fragments arranged by size:
2042 1623 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2013 2247 2924 4072 4317 1606
Size: 407 234 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 407 245 234
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4831
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4831
NotI GC'GGCC_GC
Cuts at: 2278 2278
Size: 4831
NspI r_CATG'y
Cuts at: 1132 1229 2189 3734 1132
Size: 97 960 1545 2229
Fragments arranged by size:
2229 1545 960 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4831
PciI A'CATG_T
Cuts at: 3730 3730
Size: 4831
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2037 1108
Size: 929 3902
PmeI GTTT'AAAC
Cuts at: 2135 2135
Size: 4831
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 4413 4445 2900
Size: 32 1481 32 3286
Fragments arranged by size:
3286 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4831
PsiI TTA'TAA
Cuts at: 2401 2401
Size: 4831
PstI C_TGCA'G
Cuts at: 830 2183 830
Size: 1353 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4831
SalI G'TCGA_C
Cuts at: 1936 2173 1936
Size: 237 4594
SbfI CC_TGCA'GG
Cuts at: 2183 2183
Size: 4831
ScaI AGT'ACT
Cuts at: 3079 4791 3079
Size: 1712 3119
SfcI C'TryA_G
Cuts at: 826 1045 2179 3314 3995 4186 826
Size: 219 1134 1135 681 191 1471
Fragments arranged by size:
1471 1135 1134 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4831
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4831
SmaI CCC'GGG
Cuts at: 1872 2158 1872
Size: 286 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2935 3836 4098 820
Size: 197 926 992 901 262 1553
Fragments arranged by size:
1553 992 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2622 493
Size: 2129 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4831
SphI G_CATG'C
Cuts at: 1132 2189 1132
Size: 1057 3774
SspI AAT'ATT
Cuts at: 5 52 2755 5
Size: 47 2703 2081
Fragments arranged by size:
2703 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4831
StyI C'CwwG_G
Cuts at: 513 1065 2270 513
Size: 552 1205 3074
Fragments arranged by size:
3074 1205 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2858 2875 3028 3213 3658 325
Size: 1255 1278 17 153 185 445 1498
Fragments arranged by size:
1498 1278 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3077 4789 96
Size: 274 80 33 51 1443 1100 1712 138
Fragments arranged by size:
1712 1443 1100 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4791 4823 4791
Size: 32 4799
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4831
XbaI T'CTAG_A
Cuts at: 2167 2167
Size: 4831
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2082 2082
Size: 4831
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4831
XmaI C'CCGG_G
Cuts at: 1870 2156 1870
Size: 286 4545
XmnI GAAnn'nnTTC
Cuts at: 2960 2960
Size: 4831
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2639 276
Size: 53 83 186 936 1105 2468
Fragments arranged by size:
2468 1105 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI