Restriction map for
pFN21AE1199
(Circular) (Six-base) MAPSORT of: pFN21AE1199.seq Check: 2813 from: 1 to: 5809
pFN21AE1199 5809 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2047 2047
Size: 5809
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3619 278
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2433 3152 1937
Size: 496 719 4594
Fragments arranged by size:
4594 719 496
Acc65I G'GTAC_C
Cuts at: 3130 3130
Size: 5809
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 2288 3872 5256 5524 1632
Size: 593 63 1584 1384 268 1917
Fragments arranged by size:
1917 1584 1384 593 268 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 4708 5685 1203
Size: 3505 977 1327
Fragments arranged by size:
3505 1327 977
AgeI A'CCGG_T
Cuts at: 4640 4640
Size: 5809
AhdI GACnn_n'nnGTC
Cuts at: 4538 4538
Size: 5809
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5809
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 5777
AlwNI CAG_nnn'CTG
Cuts at: 2408 3552 5124 2408
Size: 1144 1572 3093
Fragments arranged by size:
3093 1572 1144
ApaI G_GGCC'C
Cuts at: 2541 2658 2862 2993 3069 2541
Size: 117 204 131 76 5281
Fragments arranged by size:
5281 204 131 117 76
ApaLI G'TGCA_C
Cuts at: 2552 3866 5022 2552
Size: 1314 1156 3339
Fragments arranged by size:
3339 1314 1156
ApoI r'AATT_y
Cuts at: 1493 3118 3344 3496 5605 1493
Size: 1625 226 152 2109 1697
Fragments arranged by size:
2109 1697 1625 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4363 160
Size: 872 3331 1606
Fragments arranged by size:
3331 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 2258 2658 2845 2984 3105 3134 1870
Size: 73 315 400 187 139 121 29 4545
Fragments arranged by size:
4545 400 315 187 139 121 73 29
BamHI G'GATC_C
Cuts at: 3139 3506 3139
Size: 367 5442
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2927 3130 4585 618
Size: 325 217 627 1140 203 1455 1842
Fragments arranged by size:
1842 1455 1140 627 325 217 203
BanII G_rGCy'C
Cuts at: 1391 2229 2541 2658 2862 2993 3069 3128 1391
Size: 838 312 117 204 131 76 59 4072
Fragments arranged by size:
4072 838 312 204 131 117 76 59
BbeI G_GCGC'C
Cuts at: 2931 2931
Size: 5809
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2269 2303 4000 4034 1903
Size: 34 332 34 1697 34 3678
Fragments arranged by size:
3678 1697 332 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2406 3701 4911 2406
Size: 1295 1210 3304
Fragments arranged by size:
3304 1295 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5809
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3058 4420 136
Size: 107 122 71 631 605 1386 1362 1525
Fragments arranged by size:
1525 1386 1362 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2025 5804 2025
Size: 3779 2030
BlpI GC'TnA_GC
Cuts at: 2108 3226 2108
Size: 1118 4691
Bme1580I G_kGCm'C
Cuts at: 1790 2541 2556 2658 2862 2993 3069 3870 5026
Size: 751 15 102 204 131 76 801 1156
Cuts at: 5026 1790
Size: 2573
Fragments arranged by size:
2573 1156 801 751 204 131 102 76
15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2460 2747 2877
Size: 416 53 83 399 24 1043 287 130
Cuts at: 2877 2942 3540 4498 442
Size: 65 598 958 1753
Fragments arranged by size:
1753 1043 958 598 416 399 287 130
83 65 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2989 2989
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2324 2662 4469 1239
Size: 1085 338 1807 2579
Fragments arranged by size:
2579 1807 1085 338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3934 4799 5097 3934
Size: 865 298 4646
Fragments arranged by size:
4646 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2781 4472 882
Size: 617 1282 1691 2219
Fragments arranged by size:
2219 1691 1282 617
BsaAI yAC'GTr
Cuts at: 493 1204 3600 493
Size: 711 2396 2702
Fragments arranged by size:
2702 2396 711
BsaBI GATnn'nnATC
Cuts at: 5803 5803
Size: 5809
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2928 3616 3998 4633
Size: 53 83 186 936 1395 688 382 635
Cuts at: 4633 275
Size: 1451
Fragments arranged by size:
1451 1395 936 688 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4242 4640 4914 5061 5472 4242
Size: 398 274 147 411 4579
Fragments arranged by size:
4579 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2696 2707 2726 2737 1178
Size: 30 165 30 1293 11 19 11 4250
Fragments arranged by size:
4250 1293 165 30 30 19 11 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 2921 3020 1186
Size: 1153 582 99 3975
Fragments arranged by size:
3975 1153 582 99
BseYI C'CCAG_C
Cuts at: 2417 2651 2877 5012 5508 2417
Size: 234 226 2135 496 2718
Fragments arranged by size:
2718 2135 496 234 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5236
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3259 4020 5048 1540
Size: 275 186 1258 761 1028 2301
Fragments arranged by size:
2301 1258 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 2556 3870 3955 5026 2118
Size: 438 1314 85 1071 2901
Fragments arranged by size:
2901 1314 1071 438 85
BsmI GAATG_Cn'
Cuts at: 3320 3413 3320
Size: 93 5716
BsmBI CGTCTCn'nnnn_
Cuts at: 2255 2255
Size: 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2229 2541 2556 2658 2862 2993
Size: 399 328 111 312 15 102 204 131
Cuts at: 2993 3069 3128 3870 3955 5026 1391
Size: 76 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 312 204 131
111 102 85 76 59 15
BspEI T'CCGG_A
Cuts at: 5472 5472
Size: 5809
BspHI T'CATG_A
Cuts at: 3698 5428 3698
Size: 1730 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2047 2616 2643 3164 844
Size: 1203 569 27 521 3489
Fragments arranged by size:
3489 1203 569 521 27
BsrBI CCG'CTC
Cuts at: 1394 1823 3696 1394
Size: 429 1873 3507
Fragments arranged by size:
3507 1873 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4304 4478 65
Size: 1183 3056 174 1396
Fragments arranged by size:
3056 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3078 4453 4640 1700
Size: 110 1268 1375 187 2869
Fragments arranged by size:
2869 1375 1268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5809
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5809
BssSI C'ACGA_G
Cuts at: 2231 3869 4881 2231
Size: 1638 1012 3159
Fragments arranged by size:
3159 1638 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2408 794
Size: 1614 4195
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5809
BstXI CCAn_nnnn'nTGG
Cuts at: 2885 2885
Size: 5809
BstYI r'GATC_y
Cuts at: 1972 2025 3139 3506 3891 3908 5360 5446 5458
Size: 53 1114 367 385 17 1452 86 12
Cuts at: 5458 5804 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1114 385 367 346 86 53
17 12
BstZ17I GTA'TAC
Cuts at: 2434 2434
Size: 5809
Bsu36I CC'TnA_GG
Cuts at: 2322 2322
Size: 5809
BtgI C'CryG_G
Cuts at: 513 1065 2004 2923 513
Size: 552 939 919 3399
Fragments arranged by size:
3399 939 919 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5070
Fragments arranged by size:
5070 683 56
BtsI GCAGTG_nn'
Cuts at: 2483 2715 3325 4119 4139 2483
Size: 232 610 794 20 4153
Fragments arranged by size:
4153 794 610 232 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5774
DraI TTT'AAA
Cuts at: 3113 3113
Size: 5809
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4816 809
Size: 507 3500 1802
Fragments arranged by size:
3500 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3090 3256 4145
Size: 54 1425 50 275 18 1260 166 889
Cuts at: 4145 5774 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1260 889 275 166 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3256 1537
Size: 275 1444 4090
Fragments arranged by size:
4090 1444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2171 2567 3739 1353
Size: 818 396 1172 3423
Fragments arranged by size:
3423 1172 818 396
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2638 4396 4770 4916 387
Size: 1064 138 1049 1758 374 146 1280
Fragments arranged by size:
1758 1280 1064 1049 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2288 2324 2662 3872 4469 5256
Size: 393 593 63 36 338 1210 597 787
Cuts at: 5256 5524 1239
Size: 268 1524
Fragments arranged by size:
1524 1210 787 597 593 393 338 268
63 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2903 2903
Size: 5809
EcoO109I rG'GnC_Cy
Cuts at: 2318 2439 2538 2655 2765 2859 2912 2989 2990
Size: 121 99 117 110 94 53 77 1
Cuts at: 2990 2318
Size: 5137
Fragments arranged by size:
5137 121 117 110 99 94 77 53
1
EcoRI G'AATT_C
Cuts at: 3118 3496 3118
Size: 378 5431
FspI TGC'GCA
Cuts at: 4315 4315
Size: 5809
HaeII r_GCGC'y
Cuts at: 1678 2157 2931 2971 4956 1678
Size: 479 774 40 1985 2531
Fragments arranged by size:
2531 1985 774 479 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4450 4482 4524 4556 1373
Size: 32 135 32 2878 32 42 32 2626
Fragments arranged by size:
2878 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3153 3399 669
Size: 988 281 1215 246 3079
Fragments arranged by size:
3079 1215 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5809
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KasI G'GCGC_C
Cuts at: 2927 2927
Size: 5809
KpnI G_GTAC'C
Cuts at: 3134 3134
Size: 5809
MfeI C'AATT_G
Cuts at: 3408 3408
Size: 5809
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2539 4923 5107 1305
Size: 1234 2384 184 2007
Fragments arranged by size:
2384 2007 1234 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3987
Fragments arranged by size:
3987 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 3767 4126 4285 518
Size: 648 1020 1581 359 159 2042
Fragments arranged by size:
2042 1581 1020 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2925 3225 3902 5050 5295 1606
Size: 1319 300 677 1148 245 2120
Fragments arranged by size:
2120 1319 1148 677 300 245
NaeI GCC'GGC
Cuts at: 3080 3080
Size: 5809
NarI GG'CG_CC
Cuts at: 2928 2928
Size: 5809
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4318
Fragments arranged by size:
4318 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5809
NgoMIV G'CCGG_C
Cuts at: 3078 3078
Size: 5809
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 3256 3256
Size: 5809
NspI r_CATG'y
Cuts at: 1132 1229 3167 4712 1132
Size: 97 1938 1545 2229
Fragments arranged by size:
2229 1938 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5809
PciI A'CATG_T
Cuts at: 4708 4708
Size: 5809
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2777 1108
Size: 1669 4140
PfoI T'CCnGG_A
Cuts at: 2037 2725 2037
Size: 688 5121
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 3878 3910 5391 5423 2696
Size: 32 1150 32 1481 32 3082
Fragments arranged by size:
3082 1481 1150 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2912 2912
Size: 5809
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5809
PsiI TTA'TAA
Cuts at: 3379 3379
Size: 5809
PspOMI G'GGCC_C
Cuts at: 2537 2654 2858 2989 3065 2537
Size: 117 204 131 76 5281
Fragments arranged by size:
5281 204 131 117 76
PspXI vC'TCGA_Gb
Cuts at: 2845 2845
Size: 5809
PstI C_TGCA'G
Cuts at: 830 2404 2703 3161 830
Size: 1574 299 458 3478
Fragments arranged by size:
3478 1574 458 299
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5809
SacII CC_GC'GG
Cuts at: 2926 2926
Size: 5809
SalI G'TCGA_C
Cuts at: 1936 3151 1936
Size: 1215 4594
SanDI GG'GwC_CC
Cuts at: 2912 2912
Size: 5809
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 4057 5769 4057
Size: 1712 4097
SfcI C'TryA_G
Cuts at: 826 1045 2400 2689 2699 3157 4292 4973 5164
Size: 219 1355 289 10 458 1135 681 191
Cuts at: 5164 826
Size: 1471
Fragments arranged by size:
1471 1355 1135 681 458 289 219 191
10
SfoI GGC'GCC
Cuts at: 2929 2929
Size: 5809
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 2986 3107 3136 1872
Size: 1114 121 29 4545
Fragments arranged by size:
4545 1114 121 29
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2258 2845 3913 4814 5076 820
Size: 197 926 315 587 1068 901 262 1553
Fragments arranged by size:
1553 1068 926 901 587 315 262 197
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5809
SphI G_CATG'C
Cuts at: 1132 3167 1132
Size: 2035 3774
SspI AAT'ATT
Cuts at: 5 52 3733 5
Size: 47 3681 2081
Fragments arranged by size:
3681 2081 47
StuI AGG'CCT
Cuts at: 1517 2099 2547 1517
Size: 582 448 4779
Fragments arranged by size:
4779 582 448
StyI C'CwwG_G
Cuts at: 513 1065 2004 3087 3248 513
Size: 552 939 1083 161 3074
Fragments arranged by size:
3074 1083 939 552 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3836 3853 4006 4191 4636 325
Size: 1255 2256 17 153 185 445 1498
Fragments arranged by size:
2256 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 2217 4055
Size: 274 80 33 51 1443 231 9 1838
Cuts at: 4055 5767 96
Size: 1712 138
Fragments arranged by size:
1838 1712 1443 274 231 138 80 51
33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2707 2739 5769 5801 2707
Size: 32 3030 32 2715
Fragments arranged by size:
3030 2715 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5809
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2185 2425 2185
Size: 240 5569
XhoI C'TCGA_G
Cuts at: 1943 2258 2845 1943
Size: 315 587 4907
Fragments arranged by size:
4907 587 315
XmaI C'CCGG_G
Cuts at: 1870 2984 3105 3134 1870
Size: 1114 121 29 4545
Fragments arranged by size:
4545 1114 121 29
XmnI GAAnn'nnTTC
Cuts at: 3938 3938
Size: 5809
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3617 276
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PsrI PvuII RsrII SacI SapI SexAI
SfiI SrfI SwaI