Restriction map for
pFN21AE1197
(Circular) (Six-base) MAPSORT of: pFN21AE1197.seq Check: 8865 from: 1 to: 7456
pFN21AE1197 7456 bp
With 182 enzymes: SgfI *
August 27, 2010 15:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5266 278
Size: 53 83 186 936 3730 2468
Fragments arranged by size:
3730 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2034 3032 4799 1937
Size: 97 998 1767 4594
Fragments arranged by size:
4594 1767 998 97
Acc65I G'GTAC_C
Cuts at: 3504 4777 3504
Size: 1273 6183
AclI AA'CG_TT
Cuts at: 1563 5583 5956 1563
Size: 4020 373 3063
Fragments arranged by size:
4020 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2570 2934 3780 3911 4420 5519 6903 7171
Size: 938 364 846 131 509 1099 1384 268
Cuts at: 7171 1632
Size: 1917
Fragments arranged by size:
1917 1384 1099 938 846 509 364 268
131
AfeI AGC'GCT
Cuts at: 2995 2995
Size: 7456
AflII C'TTAA_G
Cuts at: 820 1017 2524 820
Size: 197 1507 5752
Fragments arranged by size:
5752 1507 197
AflIII A'CryG_T
Cuts at: 1203 3722 4138 4183 6355 7332 1203
Size: 2519 416 45 2172 977 1327
Fragments arranged by size:
2519 2172 1327 977 416 45
AgeI A'CCGG_T
Cuts at: 6287 6287
Size: 7456
AhdI GACnn_n'nnGTC
Cuts at: 6185 6185
Size: 7456
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2453 4620 1184
Size: 1269 2167 4020
Fragments arranged by size:
4020 2167 1269
AlwNI CAG_nnn'CTG
Cuts at: 2080 2408 2449 3198 3463 3973 5199 6771 2080
Size: 328 41 749 265 510 1226 1572 2765
Fragments arranged by size:
2765 1572 1226 749 510 328 265 41
ApaI G_GGCC'C
Cuts at: 2120 2510 3572 2120
Size: 390 1062 6004
Fragments arranged by size:
6004 1062 390
ApaLI G'TGCA_C
Cuts at: 5513 6669 5513
Size: 1156 6300
ApoI r'AATT_y
Cuts at: 1493 3812 4406 4472 4765 4991 5143 7252 1493
Size: 2319 594 66 293 226 152 2109 1697
Fragments arranged by size:
2319 2109 1697 594 293 226 152 66
AseI AT'TA_AT
Cuts at: 160 1032 6010 160
Size: 872 4978 1606
Fragments arranged by size:
4978 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7456
AvaI C'yCGr_G
Cuts at: 1870 1943 2113 2384 2647 4781 1870
Size: 73 170 271 263 2134 4545
Fragments arranged by size:
4545 2134 271 263 170 73
AvrII C'CTAG_G
Cuts at: 2977 2977
Size: 7456
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3758 3791 3758
Size: 33 7423
BamHI G'GATC_C
Cuts at: 4786 5153 4786
Size: 367 7089
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2124 2156 3504 4362 4777
Size: 325 217 627 337 32 1348 858 415
Cuts at: 4777 6232 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1348 858 627 415 337 325
217 32
BanII G_rGCy'C
Cuts at: 1391 2120 2510 2656 3011 3572 3718 3883 4230
Size: 729 390 146 355 561 146 165 347
Cuts at: 4230 4775 1391
Size: 545 4072
Fragments arranged by size:
4072 729 561 545 390 355 347 165
146 146
BbeI G_GCGC'C
Cuts at: 2160 2160
Size: 7456
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3674 3733 3836 928
Size: 971 1775 59 103 4548
Fragments arranged by size:
4548 1775 971 103 59
BbvCI CC'TCA_GC
Cuts at: 3286 3286
Size: 7456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3056 3090 4070 4104 4597 4631 5647
Size: 34 1119 34 980 34 493 34 1016
Cuts at: 5647 5681 1903
Size: 34 3678
Fragments arranged by size:
3678 1119 1016 980 493 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2620 5348 6558 2620
Size: 2728 1210 3518
Fragments arranged by size:
3518 2728 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7456
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2319 2420 2651
Size: 107 122 71 631 605 647 101 231
Cuts at: 2651 2864 3203 3249 6067 136
Size: 213 339 46 2818 1525
Fragments arranged by size:
2818 1525 647 631 605 339 231 213
122 107 101 71 46
BglII A'GATC_T
Cuts at: 7451 7451
Size: 7456
BlpI GC'TnA_GC
Cuts at: 4873 4873
Size: 7456
Bme1580I G_kGCm'C
Cuts at: 1790 2120 2510 2690 2860 3534 3572 4710 5517
Size: 330 390 180 170 674 38 1138 807
Cuts at: 5517 6673 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1138 807 674 390 330 180
170 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7456
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3534 4127 5187
Size: 416 53 83 399 24 2117 593 1060
Cuts at: 5187 6145 442
Size: 958 1753
Fragments arranged by size:
2117 1753 1060 958 593 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7456
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 4711 2491
Size: 2220 5236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2393 2430 3191 4258 6116 1239
Size: 1154 37 761 1067 1858 2579
Fragments arranged by size:
2579 1858 1154 1067 761 37
Bpu10I CC'TnA_GC
Cuts at: 2558 3286 2558
Size: 728 6728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4356 4540 5581 6446 6744 4356
Size: 184 1041 865 298 5068
Fragments arranged by size:
5068 1041 865 298 184
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6119 882
Size: 617 4620 2219
Fragments arranged by size:
4620 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4141 4186 5247 493
Size: 711 2937 45 1061 2702
Fragments arranged by size:
2937 2702 1061 711 45
BsaBI GATnn'nnATC
Cuts at: 7450 7450
Size: 7456
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 5263 5645 6280
Size: 53 83 186 936 624 3106 382 635
Cuts at: 6280 275
Size: 1451
Fragments arranged by size:
3106 1451 936 635 624 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2461 3053 3590 4672 5889 6287 6561 6708 7119
Size: 592 537 1082 1217 398 274 147 411
Cuts at: 7119 2461
Size: 2798
Fragments arranged by size:
2798 1217 1082 592 537 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2403 2433 1178
Size: 30 165 30 1000 30 6201
Fragments arranged by size:
6201 1000 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2079 3971 1186
Size: 893 1892 4671
Fragments arranged by size:
4671 1892 893
BseYI C'CCAG_C
Cuts at: 2023 2503 6659 7155 2023
Size: 480 4156 496 2324
Fragments arranged by size:
4156 2324 496 480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2033 3279 4616 1163
Size: 573 297 1246 1337 4003
Fragments arranged by size:
4003 1337 1246 573 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2045 4516 4906 5667 6695 1540
Size: 275 186 44 2471 390 761 1028 2301
Fragments arranged by size:
2471 2301 1028 761 390 275 186 44
BsiHKAI G_wGCw'C
Cuts at: 2912 3011 3061 3718 5517 5602 6673 2912
Size: 99 50 657 1799 85 1071 3695
Fragments arranged by size:
3695 1799 1071 657 99 85 50
BsmI GAATG_Cn'
Cuts at: 2876 4967 5060 2876
Size: 2091 93 5272
Fragments arranged by size:
5272 2091 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2510 2656 2690 2860 2912 3011
Size: 399 330 390 146 34 170 52 99
Cuts at: 3011 3061 3534 3572 3718 3883 4230 4710 4775
Size: 50 473 38 146 165 347 480 65
Cuts at: 4775 5517 5602 6673 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 480 473 399 390 347
330 170 165 146 146 99 85 65
52 50 38 34
BspEI T'CCGG_A
Cuts at: 2461 3053 3590 4672 7119 2461
Size: 592 537 1082 2447 2798
Fragments arranged by size:
2798 2447 1082 592 537
BspHI T'CATG_A
Cuts at: 5345 7075 5345
Size: 1730 5726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3680 4644 4811 844
Size: 2836 964 167 3489
Fragments arranged by size:
3489 2836 964 167
BsrBI CCG'CTC
Cuts at: 1394 1823 5343 1394
Size: 429 3520 3507
Fragments arranged by size:
3520 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4521 5951 6125 65
Size: 1183 3273 1430 174 1396
Fragments arranged by size:
3273 1430 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6100 6287 1700
Size: 110 4290 187 2869
Fragments arranged by size:
4290 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4135 4338 96
Size: 4039 203 3214
Fragments arranged by size:
4039 3214 203
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7456
BssSI C'ACGA_G
Cuts at: 5516 6528 5516
Size: 1012 6444
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2449 794
Size: 1655 5801
BstBI TT'CG_AA
Cuts at: 4489 6260 4489
Size: 1771 5685
BstEII G'GTnAC_C
Cuts at: 1184 3279 1184
Size: 2095 5361
BstXI CCAn_nnnn'nTGG
Cuts at: 2749 3344 4585 2749
Size: 595 1241 5620
Fragments arranged by size:
5620 1241 595
BstYI r'GATC_y
Cuts at: 1972 4713 4786 5153 5538 5555 7007 7093 7105
Size: 2741 73 367 385 17 1452 86 12
Cuts at: 7105 7451 1972
Size: 346 1977
Fragments arranged by size:
2741 1977 1452 385 367 346 86 73
17 12
Bsu36I CC'TnA_GG
Cuts at: 3456 3456
Size: 7456
BtgI C'CryG_G
Cuts at: 513 1065 2004 2418 2742 4242 513
Size: 552 939 414 324 1500 3727
Fragments arranged by size:
3727 1500 939 552 414 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6717
Fragments arranged by size:
6717 683 56
BtsI GCAGTG_nn'
Cuts at: 2736 3224 4424 4972 5766 5786 2736
Size: 488 1200 548 794 20 4406
Fragments arranged by size:
4406 1200 794 548 488 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7456
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7421
DraI TTT'AAA
Cuts at: 4760 4760
Size: 7456
DraIII CAC_nnn'GTG
Cuts at: 4657 4657
Size: 7456
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6463 809
Size: 507 5147 1802
Fragments arranged by size:
5147 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2042 2421 2865
Size: 54 1425 50 275 18 212 379 444
Cuts at: 2865 3117 4903 5792 7421 8
Size: 252 1786 889 1629 43
Fragments arranged by size:
1786 1629 1425 889 444 379 275 252
212 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2042 4903 1537
Size: 275 230 2861 4090
Fragments arranged by size:
4090 2861 275 230
EarI CTCTTCn'nnn_
Cuts at: 1353 3323 4439 5386 1353
Size: 1970 1116 947 3423
Fragments arranged by size:
3423 1970 1116 947
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3126 6043 6417 6563 387
Size: 1064 138 1537 2917 374 146 1280
Fragments arranged by size:
2917 1537 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3009 3716 3009
Size: 707 6749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2393 2430 2570 2934 3191 3780 3911
Size: 393 761 37 140 364 257 589 131
Cuts at: 3911 4258 4420 5519 6116 6903 7171 1239
Size: 347 162 1099 597 787 268 1524
Fragments arranged by size:
1524 1099 787 761 597 589 393 364
347 268 257 162 140 131 37
EcoNI CCTnn'n_nnAGG
Cuts at: 2336 2561 2336
Size: 225 7231
EcoO109I rG'GnC_Cy
Cuts at: 2117 2312 2507 3232 3266 3569 2117
Size: 195 195 725 34 303 6004
Fragments arranged by size:
6004 725 303 195 195 34
EcoRI G'AATT_C
Cuts at: 4406 4765 5143 4406
Size: 359 378 6719
Fragments arranged by size:
6719 378 359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3760 3760
Size: 7456
FspI TGC'GCA
Cuts at: 5962 5962
Size: 7456
HaeII r_GCGC'y
Cuts at: 1678 2160 2997 3198 6603 1678
Size: 482 837 201 3405 2531
Fragments arranged by size:
3405 2531 837 482 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2382 2414 3572 3604 6097
Size: 32 135 32 810 32 1158 32 2493
Cuts at: 6097 6129 6171 6203 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2493 1158 810 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4270 4800 5046 669
Size: 988 281 2332 530 246 3079
Fragments arranged by size:
3079 2332 988 530 281 246
HindIII A'AGCT_T
Cuts at: 748 3693 748
Size: 2945 4511
HpaI GTT'AAC
Cuts at: 5046 5046
Size: 7456
KasI G'GCGC_C
Cuts at: 2156 2156
Size: 7456
KpnI G_GTAC'C
Cuts at: 3508 4781 3508
Size: 1273 6183
MfeI C'AATT_G
Cuts at: 5055 5055
Size: 7456
MluI A'CGCG_T
Cuts at: 7332 7332
Size: 7456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2745 6570 6754 1305
Size: 1440 3825 184 2007
Fragments arranged by size:
3825 2007 1440 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5634
Fragments arranged by size:
5634 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4697 5414 5773 5932 518
Size: 648 843 2688 717 359 159 2042
Fragments arranged by size:
2688 2042 843 717 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2449 2480 2532 2540 2628 2837 3074 3181
Size: 843 31 52 8 88 209 237 107
Cuts at: 3181 3263 3758 4872 5549 6697 6942 1606
Size: 82 495 1114 677 1148 245 2120
Fragments arranged by size:
2120 1148 1114 843 677 495 245 237
209 107 88 82 52 31 8
NarI GG'CG_CC
Cuts at: 2157 2157
Size: 7456
NcoI C'CATG_G
Cuts at: 513 1065 2004 2742 4242 513
Size: 552 939 738 1500 3727
Fragments arranged by size:
3727 1500 939 738 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7456
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7456
NotI GC'GGCC_GC
Cuts at: 4903 4903
Size: 7456
NruI TCG'CGA
Cuts at: 3090 3090
Size: 7456
NspI r_CATG'y
Cuts at: 1132 1229 2809 3610 3726 4324 4530 4814 6359
Size: 97 1580 801 116 598 206 284 1545
Cuts at: 6359 1132
Size: 2229
Fragments arranged by size:
2229 1580 1545 801 598 284 206 116
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7456
PciI A'CATG_T
Cuts at: 3722 6355 3722
Size: 2633 4823
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2408 4248 4454 1108
Size: 1300 1840 206 4110
Fragments arranged by size:
4110 1840 1300 206
PfoI T'CCnGG_A
Cuts at: 2331 3011 4026 4217 4274 4331 4584 2331
Size: 680 1015 191 57 57 253 5203
Fragments arranged by size:
5203 1015 680 253 191 57 57
PmeI GTTT'AAAC
Cuts at: 4760 4760
Size: 7456
PmlI CAC'GTG
Cuts at: 4141 4186 4141
Size: 45 7411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5525 5557 7038 7070 5525
Size: 32 1481 32 5911
Fragments arranged by size:
5911 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3232 3266 3232
Size: 34 7422
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7456
PsiI TTA'TAA
Cuts at: 5026 5026
Size: 7456
PspOMI G'GGCC_C
Cuts at: 2116 2506 3568 2116
Size: 390 1062 6004
Fragments arranged by size:
6004 1062 390
PstI C_TGCA'G
Cuts at: 830 2178 3001 4808 830
Size: 1348 823 1807 3478
Fragments arranged by size:
3478 1807 1348 823
PvuI CG_AT'CG
Cuts at: 2001 4516 2001
Size: 2515 4941
PvuII CAG'CTG
Cuts at: 2449 2480 2532 3074 2449
Size: 31 52 542 6831
Fragments arranged by size:
6831 542 52 31
RsrII CG'GwC_CG
Cuts at: 3122 3122
Size: 7456
SacI G_AGCT'C
Cuts at: 3011 3718 3011
Size: 707 6749
SalI G'TCGA_C
Cuts at: 1936 4798 1936
Size: 2862 4594
SanDI GG'GwC_CC
Cuts at: 3266 3266
Size: 7456
SbfI CC_TGCA'GG
Cuts at: 4808 4808
Size: 7456
ScaI AGT'ACT
Cuts at: 4214 5704 7416 4214
Size: 1490 1712 4254
Fragments arranged by size:
4254 1712 1490
SexAI A'CCwGG_T
Cuts at: 2325 2325
Size: 7456
SfcI C'TryA_G
Cuts at: 826 1045 2174 2997 3426 3914 4804 5939 6620
Size: 219 1129 823 429 488 890 1135 681
Cuts at: 6620 6811 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1129 890 823 681 488 429
219 191
SfoI GGC'GCC
Cuts at: 2158 2158
Size: 7456
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7456
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7456
SmaI CCC'GGG
Cuts at: 1872 2115 4783 1872
Size: 243 2668 4545
Fragments arranged by size:
4545 2668 243
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2524 4371 4555 5560 6461 6723
Size: 197 926 581 1847 184 1005 901 262
Cuts at: 6723 820
Size: 1553
Fragments arranged by size:
1847 1553 1005 926 901 581 262 197
184
SnaBI TAC'GTA
Cuts at: 493 5247 493
Size: 4754 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7456
SphI G_CATG'C
Cuts at: 1132 4324 4530 4814 1132
Size: 3192 206 284 3774
Fragments arranged by size:
3774 3192 284 206
SspI AAT'ATT
Cuts at: 5 52 5380 5
Size: 47 5328 2081
Fragments arranged by size:
5328 2081 47
StuI AGG'CCT
Cuts at: 1517 2645 4160 1517
Size: 1128 1515 4813
Fragments arranged by size:
4813 1515 1128
StyI C'CwwG_G
Cuts at: 513 1065 2004 2667 2712 2742 2977 4155 4242
Size: 552 939 663 45 30 235 1178 87
Cuts at: 4242 4895 513
Size: 653 3074
Fragments arranged by size:
3074 1178 939 663 653 552 235 87
45 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2142 2741 3690 4766 5483 5500 5653
Size: 1255 562 599 949 1076 717 17 153
Cuts at: 5653 5838 6283 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1076 949 717 599 562 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4135 4212 4338
Size: 274 80 33 51 1443 2158 77 126
Cuts at: 4338 5702 7414 96
Size: 1364 1712 138
Fragments arranged by size:
2158 1712 1443 1364 274 138 126 80
77 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2403 2435 4397 4429 7416 7448 2403
Size: 32 1962 32 2987 32 2411
Fragments arranged by size:
2987 2411 1962 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3979 1316
Size: 2663 4793
XbaI T'CTAG_A
Cuts at: 4792 4792
Size: 7456
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 2323
Size: 7456
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7456
XmaI C'CCGG_G
Cuts at: 1870 2113 4781 1870
Size: 243 2668 4545
Fragments arranged by size:
4545 2668 243
XmnI GAAnn'nnTTC
Cuts at: 2081 2790 5585 2081
Size: 709 2795 3952
Fragments arranged by size:
3952 2795 709
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5264 276
Size: 53 83 186 936 3730 2468
Fragments arranged by size:
3730 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BfrBI BsiWI BsmBI BstZ17I EcoRV
FseI FspAI NaeI NgoMIV NsiI PacI PspXI PsrI
SacII SapI SfiI SrfI SwaI