Restriction map for
pFN21AE1191
(Circular) (Six-base) MAPSORT of: pFN21AE1191.seq Check: 4015 from: 1 to: 7177
pFN21AE1191 7177 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3807 3961 3807
Size: 154 7023
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4987 278
Size: 53 83 186 936 3451 2468
Fragments arranged by size:
3451 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4520 1937
Size: 2583 4594
Acc65I G'GTAC_C
Cuts at: 4498 4498
Size: 7177
AclI AA'CG_TT
Cuts at: 1563 5304 5677 1563
Size: 3741 373 3063
Fragments arranged by size:
3741 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3652 3985 5240 6624 6892 1632
Size: 2020 333 1255 1384 268 1917
Fragments arranged by size:
2020 1917 1384 1255 333 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6980
AflIII A'CryG_T
Cuts at: 1203 6076 7053 1203
Size: 4873 977 1327
Fragments arranged by size:
4873 1327 977
AgeI A'CCGG_T
Cuts at: 2946 6008 2946
Size: 3062 4115
AhdI GACnn_n'nnGTC
Cuts at: 2961 4109 5906 2961
Size: 1148 1797 4232
Fragments arranged by size:
4232 1797 1148
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7177
AlwNI CAG_nnn'CTG
Cuts at: 2911 3094 3442 3799 4920 6492 2911
Size: 183 348 357 1121 1572 3596
Fragments arranged by size:
3596 1572 1121 357 348 183
ApaI G_GGCC'C
Cuts at: 3752 3752
Size: 7177
ApaLI G'TGCA_C
Cuts at: 3040 5234 6390 3040
Size: 2194 1156 3827
Fragments arranged by size:
3827 2194 1156
ApoI r'AATT_y
Cuts at: 1493 2255 2386 2395 2546 2570 2581 2753 2783
Size: 762 131 9 151 24 11 172 30
Cuts at: 2783 4486 4712 4864 6973 1493
Size: 1703 226 152 2109 1697
Fragments arranged by size:
2109 1703 1697 762 226 172 152 151
131 30 24 11 9
AseI AT'TA_AT
Cuts at: 160 1032 5731 160
Size: 872 4699 1606
Fragments arranged by size:
4699 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
AvaI C'yCGr_G
Cuts at: 1870 1943 3026 3202 3368 3740 4058 4473 4502
Size: 73 1083 176 166 372 318 415 29
Cuts at: 4502 1870
Size: 4545
Fragments arranged by size:
4545 1083 415 372 318 176 166 73
29
BamHI G'GATC_C
Cuts at: 3546 4507 4874 3546
Size: 961 367 5849
Fragments arranged by size:
5849 961 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2656 3197 4303 4498 5953
Size: 325 217 627 869 541 1106 195 1455
Cuts at: 5953 618
Size: 1842
Fragments arranged by size:
1842 1455 1106 869 627 541 325 217
195
BanII G_rGCy'C
Cuts at: 1391 2100 2889 3155 3375 3585 3752 4496 1391
Size: 709 789 266 220 210 167 744 4072
Fragments arranged by size:
4072 789 744 709 266 220 210 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3111 928
Size: 971 1212 4994
Fragments arranged by size:
4994 1212 971
BbvCI CC'TCA_GC
Cuts at: 3133 3133
Size: 7177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3179 3213 3471 3505 5368 5402 1903
Size: 34 1242 34 258 34 1863 34 3678
Fragments arranged by size:
3678 1863 1242 258 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2816 3667 5069 6279 2816
Size: 851 1402 1210 3714
Fragments arranged by size:
3714 1402 1210 851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7177
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5788 136
Size: 107 122 71 631 605 4116 1525
Fragments arranged by size:
4116 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3768 7172 3768
Size: 3404 3773
BlpI GC'TnA_GC
Cuts at: 3374 3693 4031 4594 3374
Size: 319 338 563 5957
Fragments arranged by size:
5957 563 338 319
Bme1580I G_kGCm'C
Cuts at: 1790 3044 3752 5238 6394 1790
Size: 1254 708 1486 1156 2573
Fragments arranged by size:
2573 1486 1254 1156 708
BmgBI CAC'GTC
Cuts at: 1318 3193 1318
Size: 1875 5302
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4908 5866 442
Size: 416 53 83 399 24 3491 958 1753
Fragments arranged by size:
3491 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2058 2058
Size: 7177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2903 4445 5837 1239
Size: 1664 1542 1392 2579
Fragments arranged by size:
2579 1664 1542 1392
Bpu10I CC'TnA_GC
Cuts at: 3133 3662 4019 3133
Size: 529 357 6291
Fragments arranged by size:
6291 529 357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3139 3223 5302 6167 6465 3139
Size: 84 2079 865 298 3851
Fragments arranged by size:
3851 2079 865 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5840 882
Size: 617 4341 2219
Fragments arranged by size:
4341 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4968 493
Size: 711 3764 2702
Fragments arranged by size:
3764 2702 711
BsaBI GATnn'nnATC
Cuts at: 7171 7171
Size: 7177
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3233 4984 5366 6001
Size: 53 83 186 936 1700 1751 382 635
Cuts at: 6001 275
Size: 1451
Fragments arranged by size:
1751 1700 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2946 3564 5610 6008 6282 6429 6840 2946
Size: 618 2046 398 274 147 411 3283
Fragments arranged by size:
3283 2046 618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3274 3304 3423 3453 3457
Size: 30 165 30 1871 30 119 30 4
Cuts at: 3457 3487 1178
Size: 30 4868
Fragments arranged by size:
4868 1871 165 119 30 30 30 30
30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3121 3763 1186
Size: 1935 642 4600
Fragments arranged by size:
4600 1935 642
BseYI C'CCAG_C
Cuts at: 2262 2842 3678 3735 3989 4220 4396 4407 6380
Size: 580 836 57 254 231 176 11 1973
Cuts at: 6380 6876 2262
Size: 496 2563
Fragments arranged by size:
2563 1973 836 580 496 254 231 176
57 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4242 1163
Size: 573 2506 4098
Fragments arranged by size:
4098 2506 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4627 5388 6416 1540
Size: 275 186 1876 750 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 750 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2809 2889 3044 3467 3585 4215 5238 5323
Size: 709 80 155 423 118 630 1023 85
Cuts at: 5323 6394 2100
Size: 1071 2883
Fragments arranged by size:
2883 1071 1023 709 630 423 155 118
85 80
BsmI GAATG_Cn'
Cuts at: 4688 4781 4688
Size: 93 7084
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2809 2889 3044 3155 3375 3467
Size: 399 310 709 80 155 111 220 92
Cuts at: 3467 3585 3752 4215 4496 5238 5323 6394 1391
Size: 118 167 463 281 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 709 463 399 310 281
220 167 155 118 111 92 85 80
BspEI T'CCGG_A
Cuts at: 6840 6840
Size: 7177
BspHI T'CATG_A
Cuts at: 5066 6796 5066
Size: 1730 5447
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2835 3807 3961 4285 4299 4532 844
Size: 1991 972 154 324 14 233 3489
Fragments arranged by size:
3489 1991 972 324 233 154 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3473 3879 5064 1394
Size: 429 1650 406 1185 3507
Fragments arranged by size:
3507 1650 1185 429 406
BsrDI GCAATG_nn'
Cuts at: 65 1248 2682 5672 5846 65
Size: 1183 1434 2990 174 1396
Fragments arranged by size:
2990 1434 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2946 3394 5821 6008 1700
Size: 110 1136 448 2427 187 2869
Fragments arranged by size:
2869 2427 1136 448 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7177
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7177
BssSI C'ACGA_G
Cuts at: 5237 6249 5237
Size: 1012 6165
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2914 3442 4210 4291 794
Size: 2120 528 768 81 3680
Fragments arranged by size:
3680 2120 768 528 81
BstBI TT'CG_AA
Cuts at: 5981 5981
Size: 7177
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7177
BstXI CCAn_nnnn'nTGG
Cuts at: 2149 3304 2149
Size: 1155 6022
BstYI r'GATC_y
Cuts at: 1972 2973 3546 3591 3768 4507 4874 5259 5276
Size: 1001 573 45 177 739 367 385 17
Cuts at: 5276 6728 6814 6826 7172 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1001 739 573 385 367 346
177 86 45 17 12
Bsu36I CC'TnA_GG
Cuts at: 2851 3687 2851
Size: 836 6341
BtgI C'CryG_G
Cuts at: 513 1065 2004 3477 513
Size: 552 939 1473 4213
Fragments arranged by size:
4213 1473 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4227 487
Size: 683 56 3001 3437
Fragments arranged by size:
3437 3001 683 56
BtsI GCAGTG_nn'
Cuts at: 2307 2975 3729 4693 5487 5507 2307
Size: 668 754 964 794 20 3977
Fragments arranged by size:
3977 964 794 754 668 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7177
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7142
DraI TTT'AAA
Cuts at: 4481 4481
Size: 7177
DraIII CAC_nnn'GTG
Cuts at: 3052 3052
Size: 7177
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3626 6184 809
Size: 507 2310 2558 1802
Fragments arranged by size:
2558 2310 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2892 3874 4624
Size: 54 1425 50 275 18 1062 982 750
Cuts at: 4624 5513 7142 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1062 982 889 750 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 4624 1537
Size: 275 2062 750 4090
Fragments arranged by size:
4090 2062 750 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3573 5107 1353
Size: 2220 1534 3423
Fragments arranged by size:
3423 2220 1534
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5764 6138 6284 387
Size: 1064 138 4175 374 146 1280
Fragments arranged by size:
4175 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2098 2887 3583 2098
Size: 789 696 5692
Fragments arranged by size:
5692 789 696
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2903 3652 3985 4445 5240 5837 6624
Size: 393 1271 749 333 460 795 597 787
Cuts at: 6624 6892 1239
Size: 268 1524
Fragments arranged by size:
1524 1271 795 787 749 597 460 393
333 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3614 3614
Size: 7177
EcoO109I rG'GnC_Cy
Cuts at: 3121 3749 4062 4371 3121
Size: 628 313 309 5927
Fragments arranged by size:
5927 628 313 309
EcoRI G'AATT_C
Cuts at: 2255 2753 4486 4864 2255
Size: 498 1733 378 4568
Fragments arranged by size:
4568 1733 498 378
EcoRV GAT'ATC
Cuts at: 3822 3822
Size: 7177
FspI TGC'GCA
Cuts at: 5683 5683
Size: 7177
HaeII r_GCGC'y
Cuts at: 1678 3915 4179 6324 1678
Size: 2237 264 2145 2531
Fragments arranged by size:
2531 2237 2145 264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 2947 2979 3098
Size: 32 135 32 881 32 462 32 119
Cuts at: 3098 3130 3137 3169 4095 4127 5818 5850 5892
Size: 32 7 32 926 32 1691 32 42
Cuts at: 5892 5924 1373
Size: 32 2626
Fragments arranged by size:
2626 1691 926 881 462 135 119 42
32 32 32 32 32 32 32 32
32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2737 4521 4767 669
Size: 988 281 799 1784 246 3079
Fragments arranged by size:
3079 1784 988 799 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7177
HpaI GTT'AAC
Cuts at: 4767 4767
Size: 7177
KpnI G_GTAC'C
Cuts at: 4502 4502
Size: 7177
MfeI C'AATT_G
Cuts at: 4776 4776
Size: 7177
MluI A'CGCG_T
Cuts at: 7053 7053
Size: 7177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 2253 2349 2576 3648 6291 6475 1305
Size: 825 123 96 227 1072 2643 184 2007
Fragments arranged by size:
2643 2007 1072 825 227 184 123 96
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2894 10
Size: 54 1425 343 1062 4293
Fragments arranged by size:
4293 1425 1062 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2874 3269 5135 5494 5653 518
Size: 648 1708 395 1866 359 159 2042
Fragments arranged by size:
2042 1866 1708 648 395 359 159
MspA1I CmG'CkG
Cuts at: 1606 2266 3538 3653 3719 3873 3920 4593 5270
Size: 660 1272 115 66 154 47 673 677
Cuts at: 5270 6418 6663 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1272 1148 677 673 660 245 154
115 66 47
NaeI GCC'GGC
Cuts at: 3396 3396
Size: 7177
NcoI C'CATG_G
Cuts at: 513 1065 2004 3477 513
Size: 552 939 1473 4213
Fragments arranged by size:
4213 1473 939 552
NdeI CA'TA_TG
Cuts at: 387 2448 387
Size: 2061 5116
NgoMIV G'CCGG_C
Cuts at: 3394 3394
Size: 7177
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7177
NotI GC'GGCC_GC
Cuts at: 3874 4624 3874
Size: 750 6427
NspI r_CATG'y
Cuts at: 1132 1229 3508 4535 6080 1132
Size: 97 2279 1027 1545 2229
Fragments arranged by size:
2279 2229 1545 1027 97
PasI CC'CwG_GG
Cuts at: 1348 3829 4375 1348
Size: 2481 546 4150
Fragments arranged by size:
4150 2481 546
PciI A'CATG_T
Cuts at: 6076 6076
Size: 7177
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7177
PfoI T'CCnGG_A
Cuts at: 3594 3615 3594
Size: 21 7156
PmeI GTTT'AAAC
Cuts at: 4481 4481
Size: 7177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5246 5278 6759 6791 5246
Size: 32 1481 32 5632
Fragments arranged by size:
5632 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4062 4371 4062
Size: 309 6868
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7177
PsiI TTA'TAA
Cuts at: 2430 4747 2430
Size: 2317 4860
PspOMI G'GGCC_C
Cuts at: 3748 3748
Size: 7177
PstI C_TGCA'G
Cuts at: 830 2985 3462 3555 3645 3724 4254 4296 4529
Size: 2155 477 93 90 79 530 42 233
Cuts at: 4529 830
Size: 3478
Fragments arranged by size:
3478 2155 530 477 233 93 90 79
42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7177
PvuII CAG'CTG
Cuts at: 2266 3653 3719 3920 2266
Size: 1387 66 201 5523
Fragments arranged by size:
5523 1387 201 66
SacI G_AGCT'C
Cuts at: 2100 2889 3585 2100
Size: 789 696 5692
Fragments arranged by size:
5692 789 696
SalI G'TCGA_C
Cuts at: 1936 4519 1936
Size: 2583 4594
SanDI GG'GwC_CC
Cuts at: 4371 4371
Size: 7177
SapI GCTCTTCn'nnn_
Cuts at: 3573 3573
Size: 7177
SbfI CC_TGCA'GG
Cuts at: 3555 4296 4529 3555
Size: 741 233 6203
Fragments arranged by size:
6203 741 233
ScaI AGT'ACT
Cuts at: 5425 7137 5425
Size: 1712 5465
SfcI C'TryA_G
Cuts at: 826 1045 2981 3458 3551 3641 3704 3720 4250
Size: 219 1936 477 93 90 63 16 530
Cuts at: 4250 4292 4525 5660 6341 6532 826
Size: 42 233 1135 681 191 1471
Fragments arranged by size:
1936 1471 1135 681 530 477 233 219
191 93 90 63 42 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7177
SmaI CCC'GGG
Cuts at: 1872 3370 4060 4504 1872
Size: 1498 690 444 4545
Fragments arranged by size:
4545 1498 690 444
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3154 3238 5281 6182 6444 820
Size: 197 926 1211 84 2043 901 262 1553
Fragments arranged by size:
2043 1553 1211 926 901 262 197 84
SnaBI TAC'GTA
Cuts at: 493 4968 493
Size: 4475 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7177
SphI G_CATG'C
Cuts at: 1132 4535 1132
Size: 3403 3774
SspI AAT'ATT
Cuts at: 5 52 5101 5
Size: 47 5049 2081
Fragments arranged by size:
5049 2081 47
StuI AGG'CCT
Cuts at: 1517 4458 1517
Size: 2941 4236
StyI C'CwwG_G
Cuts at: 513 1065 2004 2353 3477 4616 513
Size: 552 939 349 1124 1139 3074
Fragments arranged by size:
3074 1139 1124 939 552 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2276 3185 3692 5204 5221 5374 5559
Size: 1255 696 909 507 1512 17 153 185
Cuts at: 5559 6004 325
Size: 445 1498
Fragments arranged by size:
1512 1498 1255 909 696 507 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5423 7135 96
Size: 274 80 33 51 1443 3446 1712 138
Fragments arranged by size:
3446 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3274 3306 3423 3455 3457 3489 7137 7169 3274
Size: 32 117 32 2 32 3648 32 3282
Fragments arranged by size:
3648 3282 117 32 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7177
XbaI T'CTAG_A
Cuts at: 4513 4513
Size: 7177
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2695 3301 2695
Size: 606 6571
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7177
XmaI C'CCGG_G
Cuts at: 1870 3368 4058 4502 1870
Size: 1498 690 444 4545
Fragments arranged by size:
4545 1498 690 444
XmnI GAAnn'nnTTC
Cuts at: 5306 5306
Size: 7177
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4985 276
Size: 53 83 186 936 3451 2468
Fragments arranged by size:
3451 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI
SfiI SfoI SrfI SwaI