Restriction map for
pFN21AE1187
(Circular) (Six-base) MAPSORT of: pFN21AE1187.seq Check: 8638 from: 1 to: 7000
pFN21AE1187 7000 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2555 2555
Size: 7000
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3402 4810 278
Size: 53 83 186 936 1866 1408 2468
Fragments arranged by size:
2468 1866 1408 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4343 1937
Size: 2406 4594
Acc65I G'GTAC_C
Cuts at: 4321 4321
Size: 7000
AclI AA'CG_TT
Cuts at: 1563 2510 5127 5500 1563
Size: 947 2617 373 3063
Fragments arranged by size:
3063 2617 947 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2580 2951 2969 3084 3153 3155 3460 5063
Size: 948 371 18 115 69 2 305 1603
Cuts at: 5063 6447 6715 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1603 1384 948 371 305 268 115
69 18 2
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6803
AflIII A'CryG_T
Cuts at: 1203 3189 3695 3864 5899 6876 1203
Size: 1986 506 169 2035 977 1327
Fragments arranged by size:
2035 1986 1327 977 506 169
AgeI A'CCGG_T
Cuts at: 5831 5831
Size: 7000
AhdI GACnn_n'nnGTC
Cuts at: 5729 5729
Size: 7000
AleI CACnn'nnGTG
Cuts at: 1166 3442 3444 3869 4173 1166
Size: 2276 2 425 304 3993
Fragments arranged by size:
3993 2276 425 304 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2547 2633 2742 1184
Size: 1363 86 109 5442
Fragments arranged by size:
5442 1363 109 86
AlwNI CAG_nnn'CTG
Cuts at: 2047 2239 2366 3392 4743 6315 2047
Size: 192 127 1026 1351 1572 2732
Fragments arranged by size:
2732 1572 1351 1026 192 127
ApaLI G'TGCA_C
Cuts at: 5057 6213 5057
Size: 1156 5844
ApoI r'AATT_y
Cuts at: 1493 2170 3100 3582 3919 3932 4309 4535 4687
Size: 677 930 482 337 13 377 226 152
Cuts at: 4687 6796 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 930 677 482 377 337 226
152 13
AseI AT'TA_AT
Cuts at: 160 1032 5554 160
Size: 872 4522 1606
Fragments arranged by size:
4522 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
AvaI C'yCGr_G
Cuts at: 1870 1943 2810 3827 4325 1870
Size: 73 867 1017 498 4545
Fragments arranged by size:
4545 1017 867 498 73
BamHI G'GATC_C
Cuts at: 4021 4330 4697 4021
Size: 309 367 6324
Fragments arranged by size:
6324 367 309
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4321 5776 618
Size: 325 217 627 2534 1455 1842
Fragments arranged by size:
2534 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3782 3801 3845 4319 1391
Size: 2391 19 44 474 4072
Fragments arranged by size:
4072 2391 474 44 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3141 3159 3514 4274 928
Size: 971 1242 18 355 760 3654
Fragments arranged by size:
3654 1242 971 760 355 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3566 3600 5191 5225 1903
Size: 34 1629 34 1591 34 3678
Fragments arranged by size:
3678 1629 1591 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4892 6102 4892
Size: 1210 5790
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7000
BfrBI ATG'CAT
Cuts at: 2796 2796
Size: 7000
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3948 5611 136
Size: 107 122 71 631 605 2276 1663 1525
Fragments arranged by size:
2276 1663 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2266 3366 3702 6995 2266
Size: 1100 336 3293 2271
Fragments arranged by size:
3293 2271 1100 336
BlpI GC'TnA_GC
Cuts at: 3800 4417 3800
Size: 617 6383
Bme1580I G_kGCm'C
Cuts at: 1790 5061 6217 1790
Size: 3271 1156 2573
Fragments arranged by size:
3271 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3867 1318
Size: 2549 4451
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2691 3687 3905
Size: 416 53 83 399 24 1274 996 218
Cuts at: 3905 4731 5689 442
Size: 826 958 1753
Fragments arranged by size:
1753 1274 996 958 826 416 399 218
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3051 4262 1056
Size: 1995 1211 3794
Fragments arranged by size:
3794 1995 1211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2024 2822 3812 4222 5660 1239
Size: 785 798 990 410 1438 2579
Fragments arranged by size:
2579 1438 990 798 785 410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5125 5990 6288 5125
Size: 865 298 5837
Fragments arranged by size:
5837 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3255 3809 5663 882
Size: 617 1756 554 1854 2219
Fragments arranged by size:
2219 1854 1756 617 554
BsaAI yAC'GTr
Cuts at: 493 1204 4791 493
Size: 711 3587 2702
Fragments arranged by size:
3587 2702 711
BsaBI GATnn'nnATC
Cuts at: 6994 6994
Size: 7000
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2877 3399 4807 5189
Size: 53 83 186 936 1344 522 1408 382
Cuts at: 5189 5824 275
Size: 635 1451
Fragments arranged by size:
1451 1408 1344 936 635 522 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5433 5831 6105 6252 6663 5433
Size: 398 274 147 411 5770
Fragments arranged by size:
5770 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3674 3704 3839 3869 1178
Size: 30 165 30 2271 30 135 30 4309
Fragments arranged by size:
4309 2271 165 135 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3809 1186
Size: 2623 4377
BseYI C'CCAG_C
Cuts at: 2838 4096 6203 6699 2838
Size: 1258 2107 496 3139
Fragments arranged by size:
3139 2107 1258 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2593 1163
Size: 573 857 5570
Fragments arranged by size:
5570 857 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4450 5211 6239 1540
Size: 275 186 2449 761 1028 2301
Fragments arranged by size:
2449 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2817 3495 3738 3801 3845 5061 5146 6217 2817
Size: 678 243 63 44 1216 85 1071 3600
Fragments arranged by size:
3600 1216 1071 678 243 85 63 44
BsmI GAATG_Cn'
Cuts at: 2138 2798 3337 4511 4604 2138
Size: 660 539 1174 93 4534
Fragments arranged by size:
4534 1174 660 539 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3113 3113
Size: 7000
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2817 3495 3738 3782 3801 3845 4319
Size: 399 1027 678 243 44 19 44 474
Cuts at: 4319 5061 5146 6217 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1027 742 678 474 399 243
85 44 44 19
BspEI T'CCGG_A
Cuts at: 6663 6663
Size: 7000
BspHI T'CATG_A
Cuts at: 4889 6619 4889
Size: 1730 5270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2555 3299 4355 844
Size: 1711 744 1056 3489
Fragments arranged by size:
3489 1711 1056 744
BsrBI CCG'CTC
Cuts at: 1394 1823 3601 3726 4887 1394
Size: 429 1778 125 1161 3507
Fragments arranged by size:
3507 1778 1161 429 125
BsrDI GCAATG_nn'
Cuts at: 65 1248 5495 5669 65
Size: 1183 4247 174 1396
Fragments arranged by size:
4247 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4100 5644 5831 1700
Size: 110 2290 1544 187 2869
Fragments arranged by size:
2869 2290 1544 187 110
BsrGI T'GTAC_A
Cuts at: 96 2250 96
Size: 2154 4846
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7000
BssSI C'ACGA_G
Cuts at: 5060 6072 5060
Size: 1012 5988
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7000
BstBI TT'CG_AA
Cuts at: 5804 5804
Size: 7000
BstEII G'GTnAC_C
Cuts at: 1184 3647 4175 1184
Size: 2463 528 4009
Fragments arranged by size:
4009 2463 528
BstXI CCAn_nnnn'nTGG
Cuts at: 2833 3989 2833
Size: 1156 5844
BstYI r'GATC_y
Cuts at: 1972 2235 2266 3366 3702 4021 4330 4697 5082
Size: 263 31 1100 336 319 309 367 385
Cuts at: 5082 5099 6551 6637 6649 6995 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1100 385 367 346 336 319
309 263 86 31 17 12
Bsu36I CC'TnA_GG
Cuts at: 4065 4065
Size: 7000
BtgI C'CryG_G
Cuts at: 513 1065 2004 2754 4168 513
Size: 552 939 750 1414 3345
Fragments arranged by size:
3345 1414 939 750 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4277 487
Size: 683 56 3051 3210
Fragments arranged by size:
3210 3051 683 56
BtsI GCAGTG_nn'
Cuts at: 3757 4112 4516 5310 5330 3757
Size: 355 404 794 20 5427
Fragments arranged by size:
5427 794 404 355 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7000
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6965
DraI TTT'AAA
Cuts at: 2113 2355 3217 4304 2113
Size: 242 862 1087 4809
Fragments arranged by size:
4809 1087 862 242
DraIII CAC_nnn'GTG
Cuts at: 3913 3913
Size: 7000
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3172 6007 809
Size: 507 1856 2835 1802
Fragments arranged by size:
2835 1856 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4131 4447 5336
Size: 54 1425 50 275 18 2301 316 889
Cuts at: 5336 6965 8
Size: 1629 43
Fragments arranged by size:
2301 1629 1425 889 316 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4447 1537
Size: 275 2635 4090
Fragments arranged by size:
4090 2635 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4930 1353
Size: 3577 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5587 5961 6107 387
Size: 1064 138 3998 374 146 1280
Fragments arranged by size:
3998 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3799 3843 3799
Size: 44 6956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2024 2580 2822 2951 2969 3084 3153
Size: 393 392 556 242 129 18 115 69
Cuts at: 3153 3155 3460 3812 4222 5063 5660 6447 6715
Size: 2 305 352 410 841 597 787 268
Cuts at: 6715 1239
Size: 1524
Fragments arranged by size:
1524 841 787 597 556 410 393 392
352 305 268 242 129 115 69 18
2
EcoRI G'AATT_C
Cuts at: 2170 3582 4309 4687 2170
Size: 1412 727 378 4483
Fragments arranged by size:
4483 1412 727 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2090 2090
Size: 7000
FspI TGC'GCA
Cuts at: 5506 5506
Size: 7000
HaeII r_GCGC'y
Cuts at: 1678 6147 1678
Size: 4469 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2326 2358 2960 2992 3839
Size: 32 135 32 754 32 602 32 847
Cuts at: 3839 3871 3929 3961 3970 4002 4006 4038 4048
Size: 32 58 32 9 32 4 32 10
Cuts at: 4048 4080 5641 5673 5715 5747 1373
Size: 32 1561 32 42 32 2626
Fragments arranged by size:
2626 1561 847 754 602 135 58 42
32 32 32 32 32 32 32 32
32 32 32 10 9 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4344 4590 669
Size: 988 281 2406 246 3079
Fragments arranged by size:
3079 2406 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7000
HpaI GTT'AAC
Cuts at: 4590 4590
Size: 7000
KpnI G_GTAC'C
Cuts at: 4325 4325
Size: 7000
MfeI C'AATT_G
Cuts at: 4599 4599
Size: 7000
MluI A'CGCG_T
Cuts at: 3189 6876 3189
Size: 3687 3313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2493 2850 2994 3846 4006 6114 6298 1305
Size: 1188 357 144 852 160 2108 184 2007
Fragments arranged by size:
2108 2007 1188 852 357 184 160 144
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4133 10
Size: 54 1425 343 2301 2877
Fragments arranged by size:
2877 2301 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2124 2205 2529 3258 3342 3442 3444
Size: 648 958 81 324 729 84 100 2
Cuts at: 3444 3869 4133 4173 4958 5317 5476 518
Size: 425 264 40 785 359 159 2042
Fragments arranged by size:
2042 958 785 729 648 425 359 324
264 159 100 84 81 40 2
MspA1I CmG'CkG
Cuts at: 1606 2028 2607 2690 3612 4416 5093 6241 6486
Size: 422 579 83 922 804 677 1148 245
Cuts at: 6486 1606
Size: 2120
Fragments arranged by size:
2120 1148 922 804 677 579 422 245
83
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5509
Fragments arranged by size:
5509 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7000
NheI G'CTAG_C
Cuts at: 1052 3047 4258 1052
Size: 1995 1211 3794
Fragments arranged by size:
3794 1995 1211
NotI GC'GGCC_GC
Cuts at: 4447 4447
Size: 7000
NsiI A_TGCA'T
Cuts at: 2798 2798
Size: 7000
NspI r_CATG'y
Cuts at: 1132 1229 2389 2534 2886 3665 3699 4358 5903
Size: 97 1160 145 352 779 34 659 1545
Cuts at: 5903 1132
Size: 2229
Fragments arranged by size:
2229 1545 1160 779 659 352 145 97
34
PasI CC'CwG_GG
Cuts at: 1348 4183 1348
Size: 2835 4165
PciI A'CATG_T
Cuts at: 3695 5899 3695
Size: 2204 4796
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3913 4103 1108
Size: 2805 190 4005
Fragments arranged by size:
4005 2805 190
PfoI T'CCnGG_A
Cuts at: 2238 2799 2238
Size: 561 6439
PmeI GTTT'AAAC
Cuts at: 4304 4304
Size: 7000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5069 5101 6582 6614 5069
Size: 32 1481 32 5455
Fragments arranged by size:
5455 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7000
PsiI TTA'TAA
Cuts at: 4570 4570
Size: 7000
PstI C_TGCA'G
Cuts at: 830 2478 4119 4278 4352 830
Size: 1648 1641 159 74 3478
Fragments arranged by size:
3478 1648 1641 159 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7000
PvuII CAG'CTG
Cuts at: 2028 2607 2690 3612 2028
Size: 579 83 922 5416
Fragments arranged by size:
5416 922 579 83
SacI G_AGCT'C
Cuts at: 3801 3845 3801
Size: 44 6956
SalI G'TCGA_C
Cuts at: 1936 4342 1936
Size: 2406 4594
SbfI CC_TGCA'GG
Cuts at: 4352 4352
Size: 7000
ScaI AGT'ACT
Cuts at: 5248 6960 5248
Size: 1712 5288
SexAI A'CCwGG_T
Cuts at: 4296 4296
Size: 7000
SfcI C'TryA_G
Cuts at: 826 1045 2367 2474 2742 4115 4274 4348 5483
Size: 219 1322 107 268 1373 159 74 1135
Cuts at: 5483 6164 6355 826
Size: 681 191 1471
Fragments arranged by size:
1471 1373 1322 1135 681 268 219 191
159 107 74
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7000
SmaI CCC'GGG
Cuts at: 1872 4327 1872
Size: 2455 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3827 5104 6005 6267 820
Size: 197 926 1884 1277 901 262 1553
Fragments arranged by size:
1884 1553 1277 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4791 493
Size: 4298 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7000
SphI G_CATG'C
Cuts at: 1132 2389 2534 4358 1132
Size: 1257 145 1824 3774
Fragments arranged by size:
3774 1824 1257 145
SspI AAT'ATT
Cuts at: 5 52 4924 5
Size: 47 4872 2081
Fragments arranged by size:
4872 2081 47
StuI AGG'CCT
Cuts at: 1517 2070 3130 4070 1517
Size: 553 1060 940 4447
Fragments arranged by size:
4447 1060 940 553
StyI C'CwwG_G
Cuts at: 513 1065 2004 2065 4439 513
Size: 552 939 61 2374 3074
Fragments arranged by size:
3074 2374 939 552 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3237 3924 5027 5044 5197 5382 5827
Size: 1255 1657 687 1103 17 153 185 445
Cuts at: 5827 325
Size: 1498
Fragments arranged by size:
1657 1498 1255 1103 687 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2250 2371 2399
Size: 274 80 33 51 1443 273 121 28
Cuts at: 2399 5246 6958 96
Size: 2847 1712 138
Fragments arranged by size:
2847 1712 1443 274 273 138 121 80
51 33 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2807 2839 6960 6992 2807
Size: 32 4121 32 2815
Fragments arranged by size:
4121 2815 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7000
XbaI T'CTAG_A
Cuts at: 2263 4336 2263
Size: 2073 4927
XhoI C'TCGA_G
Cuts at: 1943 3827 1943
Size: 1884 5116
XmaI C'CCGG_G
Cuts at: 1870 4325 1870
Size: 2455 4545
XmnI GAAnn'nnTTC
Cuts at: 3296 5129 3296
Size: 1833 5167
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3400 4808 276
Size: 53 83 186 936 1866 1408 2468
Fragments arranged by size:
2468 1866 1408 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstZ17I EcoNI EcoO109I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI