Restriction map for
pFN21AE1183
(Circular) (Six-base) MAPSORT of: pFN21AE1183.seq Check: 284 from: 1 to: 6814
pFN21AE1183 6814 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4084 4084
Size: 6814
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4624 278
Size: 53 83 186 936 3088 2468
Fragments arranged by size:
3088 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2040 2460 4157 1937
Size: 103 420 1697 4594
Fragments arranged by size:
4594 1697 420 103
Acc65I G'GTAC_C
Cuts at: 2520 4135 2520
Size: 1615 5199
AclI AA'CG_TT
Cuts at: 1563 4941 5314 1563
Size: 3378 373 3063
Fragments arranged by size:
3378 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3060 3602 4877 6261 6529 1632
Size: 1428 542 1275 1384 268 1917
Fragments arranged by size:
1917 1428 1384 1275 542 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6617
AflIII A'CryG_T
Cuts at: 1203 2016 2988 3936 5713 6690 1203
Size: 813 972 948 1777 977 1327
Fragments arranged by size:
1777 1327 977 972 948 813
AgeI A'CCGG_T
Cuts at: 2517 5645 2517
Size: 3128 3686
AhdI GACnn_n'nnGTC
Cuts at: 5543 5543
Size: 6814
AleI CACnn'nnGTG
Cuts at: 1166 3860 1166
Size: 2694 4120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6814
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 6782
AlwNI CAG_nnn'CTG
Cuts at: 2644 4054 4557 6129 2644
Size: 1410 503 1572 3329
Fragments arranged by size:
3329 1572 1410 503
ApaI G_GGCC'C
Cuts at: 2683 2838 2683
Size: 155 6659
ApaLI G'TGCA_C
Cuts at: 3538 4871 6027 3538
Size: 1333 1156 4325
Fragments arranged by size:
4325 1333 1156
ApoI r'AATT_y
Cuts at: 1493 4123 4349 4501 6610 1493
Size: 2630 226 152 2109 1697
Fragments arranged by size:
2630 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5368 160
Size: 872 4336 1606
Fragments arranged by size:
4336 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
AvaI C'yCGr_G
Cuts at: 1870 1943 2291 2742 4139 1870
Size: 73 348 451 1397 4545
Fragments arranged by size:
4545 1397 451 348 73
BamHI G'GATC_C
Cuts at: 4144 4511 4144
Size: 367 6447
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2520 2666 2821 3148 3453
Size: 325 217 627 733 146 155 327 305
Cuts at: 3453 3495 4135 5590 618
Size: 42 640 1455 1842
Fragments arranged by size:
1842 1455 733 640 627 327 325 305
217 155 146 42
BanII G_rGCy'C
Cuts at: 1391 2094 2683 2838 3164 3386 3572 3884 4098
Size: 703 589 155 326 222 186 312 214
Cuts at: 4098 4133 1391
Size: 35 4072
Fragments arranged by size:
4072 703 589 326 312 222 214 186
155 35
BbeI G_GCGC'C
Cuts at: 3152 3457 3152
Size: 305 6509
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2483 3002 3407 928
Size: 971 584 519 405 4335
Fragments arranged by size:
4335 971 584 519 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2152 2186 5005 5039 1903
Size: 34 215 34 2819 34 3678
Fragments arranged by size:
3678 2819 215 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2764 4706 5916 2764
Size: 1942 1210 3662
Fragments arranged by size:
3662 1942 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6814
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2813 3530 3707
Size: 107 122 71 631 605 1141 717 177
Cuts at: 3707 4063 5425 136
Size: 356 1362 1525
Fragments arranged by size:
1525 1362 1141 717 631 605 356 177
122 107 71
BglII A'GATC_T
Cuts at: 6809 6809
Size: 6814
BlpI GC'TnA_GC
Cuts at: 3683 4231 3683
Size: 548 6266
Bme1580I G_kGCm'C
Cuts at: 1790 2669 2683 2838 3542 4875 6031 1790
Size: 879 14 155 704 1333 1156 2573
Fragments arranged by size:
2573 1333 1156 879 704 155 14
BmgBI CAC'GTC
Cuts at: 1318 3937 1318
Size: 2619 4195
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3887 4545 5503
Size: 416 53 83 399 24 2470 658 958
Cuts at: 5503 442
Size: 1753
Fragments arranged by size:
2470 1753 958 658 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6814
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 6814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 2143 2366 2447 5474 1239
Size: 802 102 223 81 3027 2579
Fragments arranged by size:
3027 2579 802 223 102 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2399 3647 4939 5804 6102 2399
Size: 1248 1292 865 298 3111
Fragments arranged by size:
3111 1292 1248 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2396 2565 2582 3623 3953 5477 882
Size: 617 897 169 17 1041 330 1524 2219
Fragments arranged by size:
2219 1524 1041 897 617 330 169 17
BsaAI yAC'GTr
Cuts at: 493 1204 3013 4605 493
Size: 711 1809 1592 2702
Fragments arranged by size:
2702 1809 1592 711
BsaBI GATnn'nnATC
Cuts at: 6808 6808
Size: 6814
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3149 3454 3695 3924
Size: 53 83 186 936 1616 305 241 229
Cuts at: 3924 4621 5003 5638 275
Size: 697 382 635 1451
Fragments arranged by size:
1616 1451 936 697 635 382 305 241
229 186 83 53
BsaWI w'CCGG_w
Cuts at: 2076 2142 2272 2517 5247 5645 5919 6066 6477
Size: 66 130 245 2730 398 274 147 411
Cuts at: 6477 2076
Size: 2413
Fragments arranged by size:
2730 2413 411 398 274 245 147 130
66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2022 2052 2783 2813 2852
Size: 30 165 30 619 30 731 30 39
Cuts at: 2852 2882 3713 3743 1178
Size: 30 831 30 4249
Fragments arranged by size:
4249 831 731 619 165 39 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2102 2573 2876 3971 1186
Size: 858 58 471 303 1095 4029
Fragments arranged by size:
4029 1095 858 471 303 58
BseYI C'CCAG_C
Cuts at: 2711 3364 6017 6513 2711
Size: 653 2653 496 3012
Fragments arranged by size:
3012 2653 653 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2401 2702 3035 3407 1163
Size: 573 665 301 333 372 4570
Fragments arranged by size:
4570 665 573 372 333 301
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4264 5025 6053 1540
Size: 275 186 2263 761 1028 2301
Fragments arranged by size:
2301 2263 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 2844 3542 3684 4875 4960 6031 2094
Size: 750 698 142 1191 85 1071 2877
Fragments arranged by size:
2877 1191 1071 750 698 142 85
BsmI GAATG_Cn'
Cuts at: 4325 4418 4325
Size: 93 6721
BsmBI CGTCTCn'nnnn_
Cuts at: 3254 3254
Size: 6814
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2669 2683 2838 2844 3164 3386
Size: 399 304 575 14 155 6 320 222
Cuts at: 3386 3542 3572 3684 3884 4098 4133 4875 4960
Size: 156 30 112 200 214 35 742 85
Cuts at: 4960 6031 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 575 399 320 304 222
214 200 156 155 112 85 35 30
14 6
BspEI T'CCGG_A
Cuts at: 2076 2272 6477 2076
Size: 196 4205 2413
Fragments arranged by size:
4205 2413 196
BspHI T'CATG_A
Cuts at: 4703 6433 4703
Size: 1730 5084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2295 3513 4084 4169 844
Size: 1451 1218 571 85 3489
Fragments arranged by size:
3489 1451 1218 571 85
BsrBI CCG'CTC
Cuts at: 1394 1823 2431 2658 4701 1394
Size: 429 608 227 2043 3507
Fragments arranged by size:
3507 2043 608 429 227
BsrDI GCAATG_nn'
Cuts at: 65 1248 5309 5483 65
Size: 1183 4061 174 1396
Fragments arranged by size:
4061 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2517 3125 4014 5458 5645 1700
Size: 110 707 608 889 1444 187 2869
Fragments arranged by size:
2869 1444 889 707 608 187 110
BsrGI T'GTAC_A
Cuts at: 96 2019 96
Size: 1923 4891
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6814
BssSI C'ACGA_G
Cuts at: 4874 5886 4874
Size: 1012 5802
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2311 794
Size: 1517 5297
BstBI TT'CG_AA
Cuts at: 5618 5618
Size: 6814
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6814
BstXI CCAn_nnnn'nTGG
Cuts at: 3164 3742 3811 3164
Size: 578 69 6167
Fragments arranged by size:
6167 578 69
BstYI r'GATC_y
Cuts at: 1972 3468 3969 4144 4511 4896 4913 6365 6451
Size: 1496 501 175 367 385 17 1452 86
Cuts at: 6451 6463 6809 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1496 1452 501 385 367 346 175
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2766 2951 3355 3479 3793 4061
Size: 552 939 762 185 404 124 314 268
Cuts at: 4061 513
Size: 3266
Fragments arranged by size:
3266 939 762 552 404 314 268 185
124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2155 4015 487
Size: 683 56 929 1860 3286
Fragments arranged by size:
3286 1860 929 683 56
BtsI GCAGTG_nn'
Cuts at: 3248 4330 5124 5144 3248
Size: 1082 794 20 4918
Fragments arranged by size:
4918 1082 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6814
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6779
DraI TTT'AAA
Cuts at: 4118 4118
Size: 6814
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5821 809
Size: 507 4505 1802
Fragments arranged by size:
4505 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2103 2178
Size: 54 1425 50 275 18 177 96 75
Cuts at: 2178 2566 3172 3522 3948 4064 4261 5150 6779
Size: 388 606 350 426 116 197 889 1629
Cuts at: 6779 8
Size: 43
Fragments arranged by size:
1629 1425 889 606 426 388 350 275
197 177 116 96 75 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4261 1537
Size: 275 2449 4090
Fragments arranged by size:
4090 2449 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2250 4744 1353
Size: 897 2494 3423
Fragments arranged by size:
3423 2494 897
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2243 3922 5401 5775 5921 387
Size: 1064 138 654 1679 1479 374 146 1280
Fragments arranged by size:
1679 1479 1280 1064 654 374 146 138
EcoICRI GAG'CTC
Cuts at: 2092 2092
Size: 6814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2143 2366 2447 3060 3602 4877
Size: 393 409 102 223 81 613 542 1275
Cuts at: 4877 5474 6261 6529 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1275 787 613 597 542 409 393
268 223 102 81
EcoNI CCTnn'n_nnAGG
Cuts at: 2891 2891
Size: 6814
EcoO109I rG'GnC_Cy
Cuts at: 2680 3227 4051 2680
Size: 547 824 5443
Fragments arranged by size:
5443 824 547
EcoRI G'AATT_C
Cuts at: 4123 4501 4123
Size: 378 6436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3632 3632
Size: 6814
FspI TGC'GCA
Cuts at: 3256 5320 3256
Size: 2064 4750
HaeII r_GCGC'y
Cuts at: 1678 2730 2819 3152 3457 5961 1678
Size: 1052 89 333 305 2504 2531
Fragments arranged by size:
2531 2504 1052 333 305 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2393 2425 2852
Size: 32 135 32 450 32 339 32 427
Cuts at: 2852 2884 5455 5487 5529 5561 1373
Size: 32 2571 32 42 32 2626
Fragments arranged by size:
2626 2571 450 427 339 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3112 4087 4158 4404 669
Size: 988 281 1174 975 71 246 3079
Fragments arranged by size:
3079 1174 988 975 281 246 71
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6814
HpaI GTT'AAC
Cuts at: 4404 4404
Size: 6814
KasI G'GCGC_C
Cuts at: 3148 3453 3148
Size: 305 6509
KpnI G_GTAC'C
Cuts at: 2524 4139 2524
Size: 1615 5199
MfeI C'AATT_G
Cuts at: 4413 4413
Size: 6814
MluI A'CGCG_T
Cuts at: 6690 6690
Size: 6814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2008 2565 2728 3245 5928 6112 1305
Size: 703 557 163 517 2683 184 2007
Fragments arranged by size:
2683 2007 703 557 517 184 163
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3950 10
Size: 54 1425 343 2118 2874
Fragments arranged by size:
2874 2118 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 4772 5131 5290 518
Size: 648 2694 912 359 159 2042
Fragments arranged by size:
2694 2042 912 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2311 2344 2735 3038 3378 3574 3604 4063
Size: 705 33 391 303 340 196 30 459
Cuts at: 4063 4230 4907 6055 6300 1606
Size: 167 677 1148 245 2120
Fragments arranged by size:
2120 1148 705 677 459 391 340 303
245 196 167 33 30
NaeI GCC'GGC
Cuts at: 4016 4016
Size: 6814
NarI GG'CG_CC
Cuts at: 3149 3454 3149
Size: 305 6509
NcoI C'CATG_G
Cuts at: 513 1065 2004 2766 513
Size: 552 939 762 4561
Fragments arranged by size:
4561 939 762 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6814
NgoMIV G'CCGG_C
Cuts at: 4014 4014
Size: 6814
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6814
NotI GC'GGCC_GC
Cuts at: 4261 4261
Size: 6814
NspI r_CATG'y
Cuts at: 1132 1229 2020 2992 3241 4172 5717 1132
Size: 97 791 972 249 931 1545 2229
Fragments arranged by size:
2229 1545 972 931 791 249 97
PasI CC'CwG_GG
Cuts at: 1348 3164 1348
Size: 1816 4998
PciI A'CATG_T
Cuts at: 2016 2988 5713 2016
Size: 972 2725 3117
Fragments arranged by size:
3117 2725 972
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2644 2767 1108
Size: 1536 123 5155
Fragments arranged by size:
5155 1536 123
PmeI GTTT'AAAC
Cuts at: 4118 4118
Size: 6814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4883 4915 6396 6428 4883
Size: 32 1481 32 5269
Fragments arranged by size:
5269 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3227 3227
Size: 6814
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6814
PsiI TTA'TAA
Cuts at: 4384 4384
Size: 6814
PspOMI G'GGCC_C
Cuts at: 2679 2834 2679
Size: 155 6659
PstI C_TGCA'G
Cuts at: 830 2693 2849 4166 830
Size: 1863 156 1317 3478
Fragments arranged by size:
3478 1863 1317 156
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6814
PvuII CAG'CTG
Cuts at: 2311 2344 2735 3038 2311
Size: 33 391 303 6087
Fragments arranged by size:
6087 391 303 33
RsrII CG'GwC_CG
Cuts at: 2219 2219
Size: 6814
SacI G_AGCT'C
Cuts at: 2094 2094
Size: 6814
SacII CC_GC'GG
Cuts at: 4064 4064
Size: 6814
SalI G'TCGA_C
Cuts at: 1936 4156 1936
Size: 2220 4594
SanDI GG'GwC_CC
Cuts at: 3227 3227
Size: 6814
SbfI CC_TGCA'GG
Cuts at: 4166 4166
Size: 6814
ScaI AGT'ACT
Cuts at: 2981 5062 6774 2981
Size: 2081 1712 3021
Fragments arranged by size:
3021 2081 1712
SfcI C'TryA_G
Cuts at: 826 1045 2689 2845 4162 5297 5978 6169 826
Size: 219 1644 156 1317 1135 681 191 1471
Fragments arranged by size:
1644 1471 1317 1135 681 219 191 156
SfiI GGCCn_nnn'nGGCC
Cuts at: 3530 3530
Size: 6814
SfoI GGC'GCC
Cuts at: 3150 3455 3150
Size: 305 6509
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6814
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6814
SmaI CCC'GGG
Cuts at: 1872 4141 1872
Size: 2269 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2414 3626 4918 5819 6081 820
Size: 197 926 471 1212 1292 901 262 1553
Fragments arranged by size:
1553 1292 1212 926 901 471 262 197
SnaBI TAC'GTA
Cuts at: 493 4605 493
Size: 4112 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6814
SphI G_CATG'C
Cuts at: 1132 4172 1132
Size: 3040 3774
SspI AAT'ATT
Cuts at: 5 52 4738 5
Size: 47 4686 2081
Fragments arranged by size:
4686 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6814
StyI C'CwwG_G
Cuts at: 513 1065 2004 2766 2802 3702 4253 513
Size: 552 939 762 36 900 551 3074
Fragments arranged by size:
3074 939 900 762 552 551 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 4841 4858 5011 5196 5641 325
Size: 1255 840 2421 17 153 185 445 1498
Fragments arranged by size:
2421 1498 1255 840 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2019 2979 5060
Size: 274 80 33 51 1443 42 960 2081
Cuts at: 5060 6772 96
Size: 1712 138
Fragments arranged by size:
2081 1712 1443 960 274 138 80 51
42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6774 6806 6774
Size: 32 6782
Tth111I GACn'n_nGTC
Cuts at: 1316 2036 2407 1316
Size: 720 371 5723
Fragments arranged by size:
5723 720 371
XbaI T'CTAG_A
Cuts at: 4150 4150
Size: 6814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 2815 2113
Size: 702 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6814
XmaI C'CCGG_G
Cuts at: 1870 4139 1870
Size: 2269 4545
XmnI GAAnn'nnTTC
Cuts at: 2921 4943 2921
Size: 2022 4792
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4622 276
Size: 53 83 186 936 3088 2468
Fragments arranged by size:
3088 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI PsrI SapI SexAI SrfI
SwaI