Restriction map for
pFN21AE1179
(Circular) (Six-base) MAPSORT of: pFN21AE1179.seq Check: 7805 from: 1 to: 6772
pFN21AE1179 6772 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4582 278
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3955 4115 1937
Size: 2018 160 4594
Fragments arranged by size:
4594 2018 160
Acc65I G'GTAC_C
Cuts at: 3699 4093 3699
Size: 394 6378
AclI AA'CG_TT
Cuts at: 1563 4899 5272 1563
Size: 3336 373 3063
Fragments arranged by size:
3336 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3614 4835 6219 6487 1632
Size: 1982 1221 1384 268 1917
Fragments arranged by size:
1982 1917 1384 1221 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6575
AflIII A'CryG_T
Cuts at: 1203 5671 6648 1203
Size: 4468 977 1327
Fragments arranged by size:
4468 1327 977
AgeI A'CCGG_T
Cuts at: 5603 5603
Size: 6772
AhdI GACnn_n'nnGTC
Cuts at: 5501 5501
Size: 6772
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6772
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6772
AlwNI CAG_nnn'CTG
Cuts at: 2500 4052 4515 6087 2500
Size: 1552 463 1572 3185
Fragments arranged by size:
3185 1572 1552 463
ApaI G_GGCC'C
Cuts at: 2105 2169 2348 2454 2730 2747 2909 3071 3224
Size: 64 179 106 276 17 162 162 153
Cuts at: 3224 3242 3261 3315 3396 3693 3719 3792 3861
Size: 18 19 54 81 297 26 73 69
Cuts at: 3861 2105
Size: 5016
Fragments arranged by size:
5016 297 276 179 162 162 153 106
81 73 69 64 54 26 19 18
17
ApaLI G'TGCA_C
Cuts at: 4829 5985 4829
Size: 1156 5616
ApoI r'AATT_y
Cuts at: 1493 3249 4081 4307 4459 6568 1493
Size: 1756 832 226 152 2109 1697
Fragments arranged by size:
2109 1756 1697 832 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5326 160
Size: 872 4294 1606
Fragments arranged by size:
4294 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 2116 2243 2315 2482 3126 3495
Size: 73 164 9 127 72 167 644 369
Cuts at: 3495 3533 3738 3845 4097 1870
Size: 38 205 107 252 4545
Fragments arranged by size:
4545 644 369 252 205 167 164 127
107 73 72 38 9
AvrII C'CTAG_G
Cuts at: 2810 2810
Size: 6772
BamHI G'GATC_C
Cuts at: 2138 2292 3867 4055 4102 4469 2138
Size: 154 1575 188 47 367 4441
Fragments arranged by size:
4441 1575 367 188 154 47
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2654 2780 3455 3699 3726
Size: 325 217 627 867 126 675 244 27
Cuts at: 3726 3840 4093 5548 618
Size: 114 253 1455 1842
Fragments arranged by size:
1842 1455 867 675 627 325 253 244
217 126 114 27
BanII G_rGCy'C
Cuts at: 1391 2105 2169 2348 2454 2730 2747 2909 3071
Size: 714 64 179 106 276 17 162 162
Cuts at: 3071 3224 3242 3261 3296 3315 3396 3693 3719
Size: 153 18 19 35 19 81 297 26
Cuts at: 3719 3792 3861 3940 4091 1391
Size: 73 69 79 151 4072
Fragments arranged by size:
4072 714 297 276 179 162 162 153
151 106 81 79 73 69 64 35
26 19 19 18 17
BbeI G_GCGC'C
Cuts at: 3459 3459
Size: 6772
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3311 928
Size: 971 1412 4389
Fragments arranged by size:
4389 1412 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4963 4997 1903
Size: 34 3026 34 3678
Fragments arranged by size:
3678 3026 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4664 5874 4664
Size: 1210 5562
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6772
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2018 2227 2343
Size: 107 122 71 631 605 346 209 116
Cuts at: 2343 2913 3003 3075 3741 3891 3969 5383 136
Size: 570 90 72 666 150 78 1414 1525
Fragments arranged by size:
1525 1414 666 631 605 570 346 209
150 122 116 107 90 78 72 71
BglII A'GATC_T
Cuts at: 6767 6767
Size: 6772
BlpI GC'TnA_GC
Cuts at: 4189 4189
Size: 6772
Bme1580I G_kGCm'C
Cuts at: 1790 2105 2169 2348 2454 2730 2747 2909 3071
Size: 315 64 179 106 276 17 162 162
Cuts at: 3071 3224 3242 3261 3315 3396 3693 3719 3729
Size: 153 18 19 54 81 297 26 10
Cuts at: 3729 3792 3843 3861 4833 5989 1790
Size: 63 51 18 972 1156 2573
Fragments arranged by size:
2573 1156 972 315 297 276 179 162
162 153 106 81 64 63 54 51
26 19 18 18 17 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6772
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2796 4503 5461
Size: 416 53 83 399 24 1379 1707 958
Cuts at: 5461 442
Size: 1753
Fragments arranged by size:
1753 1707 1379 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6772
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2033 2283 2319 2682 3069 3660 3924 5432
Size: 794 250 36 363 387 591 264 1508
Cuts at: 5432 1239
Size: 2579
Fragments arranged by size:
2579 1508 794 591 387 363 264 250
36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4897 5762 6060 4897
Size: 865 298 5609
Fragments arranged by size:
5609 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3045 5435 882
Size: 617 1546 2390 2219
Fragments arranged by size:
2390 2219 1546 617
BsaAI yAC'GTr
Cuts at: 493 1204 3416 4563 493
Size: 711 2212 1147 2702
Fragments arranged by size:
2702 2212 1147 711
BsaBI GATnn'nnATC
Cuts at: 2405 3918 6766 2405
Size: 1513 2848 2411
Fragments arranged by size:
2848 2411 1513
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3276 3456 4579 4961
Size: 53 83 186 936 1743 180 1123 382
Cuts at: 4961 5596 275
Size: 635 1451
Fragments arranged by size:
1743 1451 1123 936 635 382 186 180
83 53
BsaWI w'CCGG_w
Cuts at: 5205 5603 5877 6024 6435 5205
Size: 398 274 147 411 5542
Fragments arranged by size:
5542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3278 3308 1178
Size: 30 165 30 1875 30 4642
Fragments arranged by size:
4642 1875 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 3144 1186
Size: 850 1108 4814
Fragments arranged by size:
4814 1108 850
BseYI C'CCAG_C
Cuts at: 2216 2831 3440 5975 6471 2216
Size: 615 609 2535 496 2517
Fragments arranged by size:
2535 2517 615 609 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2985 1163
Size: 573 1249 4950
Fragments arranged by size:
4950 1249 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4222 4983 6011 1540
Size: 275 186 2221 761 1028 2301
Fragments arranged by size:
2301 2221 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 4833 4918 5989 2064
Size: 2769 85 1071 2847
Fragments arranged by size:
2847 2769 1071 85
BsmI GAATG_Cn'
Cuts at: 4283 4376 4283
Size: 93 6679
BsmBI CGTCTCn'nnnn_
Cuts at: 2732 2732
Size: 6772
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2105 2169 2348 2454 2730 2747
Size: 399 274 41 64 179 106 276 17
Cuts at: 2747 2909 3071 3224 3242 3261 3296 3315 3396
Size: 162 162 153 18 19 35 19 81
Cuts at: 3396 3693 3719 3729 3792 3843 3861 3940 4091
Size: 297 26 10 63 51 18 79 151
Cuts at: 4091 4833 4918 5989 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 297 276 274 179
162 162 153 151 106 85 81 79
64 63 51 41 35 26 19 19
18 18 17 10
BspEI T'CCGG_A
Cuts at: 6435 6435
Size: 6772
BspHI T'CATG_A
Cuts at: 4661 6391 4661
Size: 1730 5042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3941 4009 4127 844
Size: 3097 68 118 3489
Fragments arranged by size:
3489 3097 118 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2098 4659 1394
Size: 429 275 2561 3507
Fragments arranged by size:
3507 2561 429 275
BsrDI GCAATG_nn'
Cuts at: 65 1248 5267 5441 65
Size: 1183 4019 174 1396
Fragments arranged by size:
4019 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3344 3885 5416 5603 1700
Size: 110 1534 541 1531 187 2869
Fragments arranged by size:
2869 1534 1531 541 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6772
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6772
BssSI C'ACGA_G
Cuts at: 4832 5844 4832
Size: 1012 5760
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6772
BstBI TT'CG_AA
Cuts at: 5576 5576
Size: 6772
BstEII G'GTnAC_C
Cuts at: 1184 3167 3329 1184
Size: 1983 162 4627
Fragments arranged by size:
4627 1983 162
BstYI r'GATC_y
Cuts at: 1972 2138 2292 3867 4055 4102 4469 4854 4871
Size: 166 154 1575 188 47 367 385 17
Cuts at: 4871 6323 6409 6421 6767 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1575 1452 385 367 346 188 166
154 86 47 17 12
Bsu36I CC'TnA_GG
Cuts at: 3207 3225 3207
Size: 18 6754
BtgI C'CryG_G
Cuts at: 513 1065 2004 2286 3316 3721 3765 3975 513
Size: 552 939 282 1030 405 44 210 3310
Fragments arranged by size:
3310 1030 939 552 405 282 210 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6033
Fragments arranged by size:
6033 683 56
BtsI GCAGTG_nn'
Cuts at: 2515 2968 4288 5082 5102 2515
Size: 453 1320 794 20 4185
Fragments arranged by size:
4185 1320 794 453 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6772
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6737
DraI TTT'AAA
Cuts at: 4076 4076
Size: 6772
DraIII CAC_nnn'GTG
Cuts at: 2798 3580 2798
Size: 782 5990
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2688 5779 809
Size: 507 1372 3091 1802
Fragments arranged by size:
3091 1802 1372 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2617 3342
Size: 54 1425 50 275 18 177 610 725
Cuts at: 3342 3504 3883 3892 4219 5108 6737 8
Size: 162 379 9 327 889 1629 43
Fragments arranged by size:
1629 1425 889 725 610 379 327 275
177 162 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 4219 1537
Size: 275 2407 4090
Fragments arranged by size:
4090 2407 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4702 1353
Size: 3349 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5359 5733 5879 387
Size: 1064 138 3770 374 146 1280
Fragments arranged by size:
3770 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2283 2319 2682 3069 3614 3660
Size: 393 401 250 36 363 387 545 46
Cuts at: 3660 3924 4835 5432 6219 6487 1239
Size: 264 911 597 787 268 1524
Fragments arranged by size:
1524 911 787 597 545 401 393 387
363 268 264 250 46 36
EcoO109I rG'GnC_Cy
Cuts at: 2101 2102 2111 2166 2192 2345 2414 2425 2450
Size: 1 9 55 26 153 69 11 25
Cuts at: 2450 2451 2459 2555 2636 2690 2726 2744 2807
Size: 1 8 96 81 54 36 18 63
Cuts at: 2807 2906 2934 3044 3076 3122 3203 3220 3221
Size: 99 28 110 32 46 81 17 1
Cuts at: 3221 3238 3239 3257 3258 3312 3392 3671 3680
Size: 17 1 18 1 54 80 279 9
Cuts at: 3680 3690 3715 3716 3788 3858 3927 3971 4067
Size: 10 25 1 72 70 69 44 96
Cuts at: 4067 2101
Size: 4806
Fragments arranged by size:
4806 279 153 110 99 96 96 81
81 80 72 70 69 69 63 55
54 54 46 44 36 32 28 26
25 25 18 18 17 17 11 10
9 9 8 1 1 1 1 1
1
EcoRI G'AATT_C
Cuts at: 3249 4081 4459 3249
Size: 832 378 5562
Fragments arranged by size:
5562 832 378
FseI GG_CCGG'CC
Cuts at: 3348 3889 3348
Size: 541 6231
FspI TGC'GCA
Cuts at: 5278 5278
Size: 6772
HaeII r_GCGC'y
Cuts at: 1678 2775 3459 3593 5919 1678
Size: 1097 684 134 2326 2531
Fragments arranged by size:
2531 2326 1097 684 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2068 2100 2429 2461 2731
Size: 32 135 32 496 32 329 32 270
Cuts at: 2731 2763 3278 3310 3658 3690 3911 3943 4040
Size: 32 515 32 348 32 221 32 97
Cuts at: 4040 4072 5413 5445 5487 5519 1373
Size: 32 1341 32 42 32 2626
Fragments arranged by size:
2626 1341 515 496 348 329 270 221
135 97 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3956 4116 4362 669
Size: 988 281 2018 160 246 3079
Fragments arranged by size:
3079 2018 988 281 246 160
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6772
HpaI GTT'AAC
Cuts at: 4362 4362
Size: 6772
KasI G'GCGC_C
Cuts at: 3455 3455
Size: 6772
KpnI G_GTAC'C
Cuts at: 3703 4097 3703
Size: 394 6378
MfeI C'AATT_G
Cuts at: 4371 4371
Size: 6772
MluI A'CGCG_T
Cuts at: 6648 6648
Size: 6772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2166 2552 5886 6070 1305
Size: 861 386 3334 184 2007
Fragments arranged by size:
3334 2007 861 386 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2619 3894 10
Size: 54 1425 343 787 1275 2888
Fragments arranged by size:
2888 1425 1275 787 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4730 5089 5248 518
Size: 648 3564 359 159 2042
Fragments arranged by size:
3564 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3318 3444 4188 4865 6013 6258 1606
Size: 1712 126 744 677 1148 245 2120
Fragments arranged by size:
2120 1712 1148 744 677 245 126
NaeI GCC'GGC
Cuts at: 3346 3887 3346
Size: 541 6231
NarI GG'CG_CC
Cuts at: 3456 3456
Size: 6772
NcoI C'CATG_G
Cuts at: 513 1065 2004 2286 3721 513
Size: 552 939 282 1435 3564
Fragments arranged by size:
3564 1435 939 552 282
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6772
NgoMIV G'CCGG_C
Cuts at: 3344 3885 3344
Size: 541 6231
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6772
NotI GC'GGCC_GC
Cuts at: 4219 4219
Size: 6772
NspI r_CATG'y
Cuts at: 1132 1229 2588 4130 5675 1132
Size: 97 1359 1542 1545 2229
Fragments arranged by size:
2229 1545 1542 1359 97
PasI CC'CwG_GG
Cuts at: 1348 2465 2561 2695 2875 3101 3145 3299 3335
Size: 1117 96 134 180 226 44 154 36
Cuts at: 3335 3461 3649 3695 3863 3932 1348
Size: 126 188 46 168 69 4188
Fragments arranged by size:
4188 1117 226 188 180 168 154 134
126 96 69 46 44 36
PciI A'CATG_T
Cuts at: 5671 5671
Size: 6772
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2500 1108
Size: 1392 5380
PfoI T'CCnGG_A
Cuts at: 2188 2374 3082 3676 3820 3940 2188
Size: 186 708 594 144 120 5020
Fragments arranged by size:
5020 708 594 186 144 120
PmeI GTTT'AAAC
Cuts at: 4076 4076
Size: 6772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4841 4873 6354 6386 4841
Size: 32 1481 32 5227
Fragments arranged by size:
5227 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2111 2192 2425 2459 2555 2636 2690 2807 2934
Size: 81 233 34 96 81 54 117 127
Cuts at: 2934 3671 3680 3927 2111
Size: 737 9 247 4956
Fragments arranged by size:
4956 737 247 233 127 117 96 81
81 54 34 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6772
PsiI TTA'TAA
Cuts at: 4342 4342
Size: 6772
PspOMI G'GGCC_C
Cuts at: 2101 2165 2344 2450 2726 2743 2905 3067 3220
Size: 64 179 106 276 17 162 162 153
Cuts at: 3220 3238 3257 3311 3392 3689 3715 3788 3857
Size: 18 19 54 81 297 26 73 69
Cuts at: 3857 2101
Size: 5016
Fragments arranged by size:
5016 297 276 179 162 162 153 106
81 73 69 64 54 26 19 18
17
PspXI vC'TCGA_Gb
Cuts at: 3126 3495 3126
Size: 369 6403
PstI C_TGCA'G
Cuts at: 830 2571 4124 830
Size: 1741 1553 3478
Fragments arranged by size:
3478 1741 1553
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6772
SacII CC_GC'GG
Cuts at: 3319 3319
Size: 6772
SalI G'TCGA_C
Cuts at: 1936 3954 4114 1936
Size: 2018 160 4594
Fragments arranged by size:
4594 2018 160
SanDI GG'GwC_CC
Cuts at: 2111 2192 2425 2636 2690 2934 3927 2111
Size: 81 233 211 54 244 993 4956
Fragments arranged by size:
4956 993 244 233 211 81 54
SbfI CC_TGCA'GG
Cuts at: 4124 4124
Size: 6772
ScaI AGT'ACT
Cuts at: 5020 6732 5020
Size: 1712 5060
SexAI A'CCwGG_T
Cuts at: 2473 2473
Size: 6772
SfcI C'TryA_G
Cuts at: 826 1045 2567 4120 5255 5936 6127 826
Size: 219 1522 1553 1135 681 191 1471
Fragments arranged by size:
1553 1522 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2227 2343 3075 3891 3969 2227
Size: 116 732 816 78 5030
Fragments arranged by size:
5030 816 732 116 78
SfoI GGC'GCC
Cuts at: 3457 3457
Size: 6772
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6772
SmaI CCC'GGG
Cuts at: 1872 2109 2118 2245 2317 2484 3535 3847 4099
Size: 237 9 127 72 167 1051 312 252
Cuts at: 4099 1872
Size: 4545
Fragments arranged by size:
4545 1051 312 252 237 167 127 72
9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3126 3495 4876 5777 6039 820
Size: 197 926 1183 369 1381 901 262 1553
Fragments arranged by size:
1553 1381 1183 926 901 369 262 197
SnaBI TAC'GTA
Cuts at: 493 4563 493
Size: 4070 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6772
SphI G_CATG'C
Cuts at: 1132 2588 4130 1132
Size: 1456 1542 3774
Fragments arranged by size:
3774 1542 1456
SrfI GCCC'GGGC
Cuts at: 2317 2317
Size: 6772
SspI AAT'ATT
Cuts at: 5 52 4696 5
Size: 47 4644 2081
Fragments arranged by size:
4644 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6772
StyI C'CwwG_G
Cuts at: 513 1065 2004 2196 2268 2286 2418 2640 2730
Size: 552 939 192 72 18 132 222 90
Cuts at: 2730 2810 3172 3262 3396 3721 3792 4012 4211
Size: 80 362 90 134 325 71 220 199
Cuts at: 4211 513
Size: 3074
Fragments arranged by size:
3074 939 552 362 325 222 220 199
192 134 132 90 90 80 72 71
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2597 2642 3317 3641 4799 4816 4969
Size: 1255 1017 45 675 324 1158 17 153
Cuts at: 4969 5154 5599 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1158 1017 675 445 324 185
153 45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5018 6730 96
Size: 274 80 33 51 1443 3041 1712 138
Fragments arranged by size:
3041 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2507 2539 2593 2625 6732 6764 2507
Size: 32 54 32 4107 32 2515
Fragments arranged by size:
4107 2515 54 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6772
XbaI T'CTAG_A
Cuts at: 4108 4108
Size: 6772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2647 3728 2497
Size: 150 1081 5541
Fragments arranged by size:
5541 1081 150
XhoI C'TCGA_G
Cuts at: 1943 3126 3495 1943
Size: 1183 369 5220
Fragments arranged by size:
5220 1183 369
XmaI C'CCGG_G
Cuts at: 1870 2107 2116 2243 2315 2482 3533 3845 4097
Size: 237 9 127 72 167 1051 312 252
Cuts at: 4097 1870
Size: 4545
Fragments arranged by size:
4545 1051 312 252 237 167 127 72
9
XmnI GAAnn'nnTTC
Cuts at: 4901 4901
Size: 6772
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4580 276
Size: 53 83 186 936 3046 2468
Fragments arranged by size:
3046 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI
FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII
SacI SapI SwaI