Restriction map for
pFN21AE1178
(Circular) (Six-base) MAPSORT of: pFN21AE1178.seq Check: 588 from: 1 to: 6763
pFN21AE1178 6763 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4573 278
Size: 53 83 186 936 3037 2468
Fragments arranged by size:
3037 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4106 1937
Size: 2169 4594
Acc65I G'GTAC_C
Cuts at: 4084 4084
Size: 6763
AclI AA'CG_TT
Cuts at: 1563 4890 5263 1563
Size: 3327 373 3063
Fragments arranged by size:
3327 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2613 2764 3016 3100 3505 3589 3673 3757
Size: 981 151 252 84 405 84 84 84
Cuts at: 3757 4826 6210 6478 1632
Size: 1069 1384 268 1917
Fragments arranged by size:
1917 1384 1069 981 405 268 252 151
84 84 84 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6566
AflIII A'CryG_T
Cuts at: 1203 5662 6639 1203
Size: 4459 977 1327
Fragments arranged by size:
4459 1327 977
AgeI A'CCGG_T
Cuts at: 5594 5594
Size: 6763
AhdI GACnn_n'nnGTC
Cuts at: 5492 5492
Size: 6763
AleI CACnn'nnGTG
Cuts at: 1166 2118 3866 1166
Size: 952 1748 4063
Fragments arranged by size:
4063 1748 952
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6763
AlwNI CAG_nnn'CTG
Cuts at: 2065 2787 4506 6078 2065
Size: 722 1719 1572 2750
Fragments arranged by size:
2750 1719 1572 722
ApaI G_GGCC'C
Cuts at: 2086 3823 2086
Size: 1737 5026
ApaLI G'TGCA_C
Cuts at: 2036 4820 5976 2036
Size: 2784 1156 2823
Fragments arranged by size:
2823 2784 1156
ApoI r'AATT_y
Cuts at: 1493 3174 3219 3966 4050 4072 4298 4450 6559
Size: 1681 45 747 84 22 226 152 2109
Cuts at: 6559 1493
Size: 1697
Fragments arranged by size:
2109 1697 1681 747 226 152 84 45
22
AseI AT'TA_AT
Cuts at: 160 1032 2655 5317 160
Size: 872 1623 2662 1606
Fragments arranged by size:
2662 1623 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6763
AvaI C'yCGr_G
Cuts at: 1870 1943 2046 2420 4088 1870
Size: 73 103 374 1668 4545
Fragments arranged by size:
4545 1668 374 103 73
BamHI G'GATC_C
Cuts at: 4093 4460 4093
Size: 367 6396
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2230 4084 5539 618
Size: 325 217 627 443 1854 1455 1842
Fragments arranged by size:
1854 1842 1455 627 443 325 217
BanII G_rGCy'C
Cuts at: 1391 2086 2876 2960 3128 3449 3785 3823 4082
Size: 695 790 84 168 321 336 38 259
Cuts at: 4082 1391
Size: 4072
Fragments arranged by size:
4072 790 695 336 321 259 168 84
38
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2770 3921 928
Size: 971 871 1151 3770
Fragments arranged by size:
3770 1151 971 871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2027 2061 4954 4988 1903
Size: 34 90 34 2893 34 3678
Fragments arranged by size:
3678 2893 90 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4655 5865 4655
Size: 1210 5553
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6763
BfrBI ATG'CAT
Cuts at: 3154 3154
Size: 6763
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5374 136
Size: 107 122 71 631 605 3702 1525
Fragments arranged by size:
3702 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6758 6758
Size: 6763
BlpI GC'TnA_GC
Cuts at: 4180 4180
Size: 6763
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2086 3823 4824 5980 1790
Size: 250 46 1737 1001 1156 2573
Fragments arranged by size:
2573 1737 1156 1001 250 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6763
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2916 3084 3168
Size: 416 53 83 399 24 1499 168 84
Cuts at: 3168 3817 4494 5452 442
Size: 649 677 958 1753
Fragments arranged by size:
1753 1499 958 677 649 416 399 168
84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6763
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 6763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2126 2195 3333 3417 3501 3669 5423 1239
Size: 887 69 1138 84 84 168 1754 2579
Fragments arranged by size:
2579 1754 1138 887 168 84 84 69
Bpu10I CC'TnA_GC
Cuts at: 2157 2870 2157
Size: 713 6050
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2944 4012 4888 5753 6051 2944
Size: 1068 876 865 298 3656
Fragments arranged by size:
3656 1068 876 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5426 882
Size: 617 3927 2219
Fragments arranged by size:
3927 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4554 493
Size: 711 3350 2702
Fragments arranged by size:
3350 2702 711
BsaBI GATnn'nnATC
Cuts at: 6757 6757
Size: 6763
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4570 4952 5587 275
Size: 53 83 186 936 3037 382 635 1451
Fragments arranged by size:
3037 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5196 5594 5868 6015 6426 5196
Size: 398 274 147 411 5533
Fragments arranged by size:
5533 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2045 2075 1178
Size: 30 165 30 642 30 5866
Fragments arranged by size:
5866 642 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2483 2615 1186
Size: 880 417 132 5334
Fragments arranged by size:
5334 880 417 132
BseYI C'CCAG_C
Cuts at: 2524 3138 4038 5966 6462 2524
Size: 614 900 1928 496 2825
Fragments arranged by size:
2825 1928 900 614 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2781 3772 1163
Size: 573 1045 991 4154
Fragments arranged by size:
4154 1045 991 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4213 4974 6002 1540
Size: 275 186 2212 761 1028 2301
Fragments arranged by size:
2301 2212 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2040 2876 2960 3128 3617 3785 4824 4909 5980
Size: 836 84 168 489 168 1039 85 1071
Cuts at: 5980 2040
Size: 2823
Fragments arranged by size:
2823 1071 1039 836 489 168 168 85
84
BsiWI C'GTAC_G
Cuts at: 2751 2751
Size: 6763
BsmI GAATG_Cn'
Cuts at: 2690 3587 3850 4274 4367 2690
Size: 897 263 424 93 5086
Fragments arranged by size:
5086 897 424 263 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2367 2367
Size: 6763
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2086 2876 2960 3128 3449 3617
Size: 399 250 46 790 84 168 321 168
Cuts at: 3617 3785 3823 4082 4824 4909 5980 1391
Size: 168 38 259 742 85 1071 2174
Fragments arranged by size:
2174 1071 790 742 399 321 259 250
168 168 168 85 84 46 38
BspEI T'CCGG_A
Cuts at: 6426 6426
Size: 6763
BspHI T'CATG_A
Cuts at: 4652 6382 4652
Size: 1730 5033
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2214 4118 844
Size: 1370 1904 3489
Fragments arranged by size:
3489 1904 1370
BsrBI CCG'CTC
Cuts at: 1394 1823 4650 1394
Size: 429 2827 3507
Fragments arranged by size:
3507 2827 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4003 5258 5432 65
Size: 1183 2755 1255 174 1396
Fragments arranged by size:
2755 1396 1255 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5407 5594 1700
Size: 110 3597 187 2869
Fragments arranged by size:
3597 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3341 96
Size: 3245 3518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6763
BssSI C'ACGA_G
Cuts at: 4823 5835 4823
Size: 1012 5751
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6763
BstBI TT'CG_AA
Cuts at: 5567 5567
Size: 6763
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6763
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6763
BstYI r'GATC_y
Cuts at: 1972 3276 4093 4460 4845 4862 6314 6400 6412
Size: 1304 817 367 385 17 1452 86 12
Cuts at: 6412 6758 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1304 817 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2192 2737 513
Size: 552 939 188 545 4539
Fragments arranged by size:
4539 939 552 545 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2040 487
Size: 683 56 814 5210
Fragments arranged by size:
5210 814 683 56
BtsI GCAGTG_nn'
Cuts at: 2066 2125 3760 4279 5073 5093 2066
Size: 59 1635 519 794 20 3736
Fragments arranged by size:
3736 1635 794 519 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6763
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3085 3120 3985 4020 529
Size: 35 2521 35 865 35 3272
Fragments arranged by size:
3272 2521 865 35 35 35
DraI TTT'AAA
Cuts at: 2839 3412 4067 2839
Size: 573 655 5535
Fragments arranged by size:
5535 655 573
DraIII CAC_nnn'GTG
Cuts at: 2850 2850
Size: 6763
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5770 809
Size: 507 4454 1802
Fragments arranged by size:
4454 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2074 4210 5099
Size: 54 1425 50 275 18 244 2136 889
Cuts at: 5099 6728 8
Size: 1629 43
Fragments arranged by size:
2136 1629 1425 889 275 244 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4210 1537
Size: 275 2398 4090
Fragments arranged by size:
4090 2398 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2181 4693 1353
Size: 828 2512 3423
Fragments arranged by size:
3423 2512 828
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2855 5350 5724 5870 387
Size: 1064 138 1266 2495 374 146 1280
Fragments arranged by size:
2495 1280 1266 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2874 2958 3126 3783 2874
Size: 84 168 657 5854
Fragments arranged by size:
5854 657 168 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2195 2613 2764 3016 3100 3333
Size: 393 494 69 418 151 252 84 233
Cuts at: 3333 3417 3501 3505 3589 3669 3673 3757 4826
Size: 84 84 4 84 80 4 84 1069
Cuts at: 4826 5423 6210 6478 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1069 787 597 494 418 393 268
252 233 151 84 84 84 84 84
80 69 4 4
EcoO109I rG'GnC_Cy
Cuts at: 2082 2083 2197 2238 3000 3084 3657 3819 2082
Size: 1 114 41 762 84 573 162 5026
Fragments arranged by size:
5026 762 573 162 114 84 41 1
EcoRI G'AATT_C
Cuts at: 3219 3966 4072 4450 3219
Size: 747 106 378 5532
Fragments arranged by size:
5532 747 378 106
EcoRV GAT'ATC
Cuts at: 2635 2635
Size: 6763
FspI TGC'GCA
Cuts at: 5269 5269
Size: 6763
HaeII r_GCGC'y
Cuts at: 1678 5910 1678
Size: 4232 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3280 3312 3772 3804 5404
Size: 32 135 32 1708 32 460 32 1600
Cuts at: 5404 5436 5478 5510 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1708 1600 460 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4107 4353 669
Size: 988 281 2169 246 3079
Fragments arranged by size:
3079 2169 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2943 3264 4011 748
Size: 2195 321 747 3500
Fragments arranged by size:
3500 2195 747 321
HpaI GTT'AAC
Cuts at: 4353 4353
Size: 6763
KpnI G_GTAC'C
Cuts at: 4088 4088
Size: 6763
MfeI C'AATT_G
Cuts at: 4362 4362
Size: 6763
MluI A'CGCG_T
Cuts at: 6639 6639
Size: 6763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3736 5877 6061 1305
Size: 2431 2141 184 2007
Fragments arranged by size:
2431 2141 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2076 10
Size: 54 1425 343 244 4697
Fragments arranged by size:
4697 1425 343 244 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2118 3244 3412 3866 3909 4721
Size: 648 843 109 1126 168 454 43 812
Cuts at: 4721 5080 5239 518
Size: 359 159 2042
Fragments arranged by size:
2042 1126 843 812 648 454 359 168
159 109 43
MspA1I CmG'CkG
Cuts at: 1606 2535 3049 3301 4179 4856 6004 6249 1606
Size: 929 514 252 878 677 1148 245 2120
Fragments arranged by size:
2120 1148 929 878 677 514 252 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2192 2737 513
Size: 552 939 188 545 4539
Fragments arranged by size:
4539 939 552 545 188
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6763
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6763
NotI GC'GGCC_GC
Cuts at: 4210 4210
Size: 6763
NsiI A_TGCA'T
Cuts at: 3156 3156
Size: 6763
NspI r_CATG'y
Cuts at: 1132 1229 4121 5666 1132
Size: 97 2892 1545 2229
Fragments arranged by size:
2892 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2509 1348
Size: 1161 5602
PciI A'CATG_T
Cuts at: 5662 5662
Size: 6763
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6763
PmeI GTTT'AAAC
Cuts at: 4067 4067
Size: 6763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4832 4864 6345 6377 4832
Size: 32 1481 32 5218
Fragments arranged by size:
5218 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2197 2238 2197
Size: 41 6722
PshAI GACnn'nnGTC
Cuts at: 1613 2327 1613
Size: 714 6049
PsiI TTA'TAA
Cuts at: 3327 3990 4333 3327
Size: 663 343 5757
Fragments arranged by size:
5757 663 343
PspOMI G'GGCC_C
Cuts at: 2082 3819 2082
Size: 1737 5026
PstI C_TGCA'G
Cuts at: 830 2211 4115 830
Size: 1381 1904 3478
Fragments arranged by size:
3478 1904 1381
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6763
PvuII CAG'CTG
Cuts at: 3049 3301 3049
Size: 252 6511
SacI G_AGCT'C
Cuts at: 2876 2960 3128 3785 2876
Size: 84 168 657 5854
Fragments arranged by size:
5854 657 168 84
SalI G'TCGA_C
Cuts at: 1936 4105 1936
Size: 2169 4594
SapI GCTCTTCn'nnn_
Cuts at: 2181 2181
Size: 6763
SbfI CC_TGCA'GG
Cuts at: 2211 4115 2211
Size: 1904 4859
ScaI AGT'ACT
Cuts at: 5011 6723 5011
Size: 1712 5051
SfcI C'TryA_G
Cuts at: 826 1045 2089 2207 2920 3228 4111 5246 5927
Size: 219 1044 118 713 308 883 1135 681
Cuts at: 5927 6118 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1044 883 713 681 308 219
191 118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6763
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6763
SmaI CCC'GGG
Cuts at: 1872 2422 4090 1872
Size: 550 1668 4545
Fragments arranged by size:
4545 1668 550
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2959 4027 4867 5768 6030 820
Size: 197 926 1016 1068 840 901 262 1553
Fragments arranged by size:
1553 1068 1016 926 901 840 262 197
SnaBI TAC'GTA
Cuts at: 493 4554 493
Size: 4061 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6763
SphI G_CATG'C
Cuts at: 1132 4121 1132
Size: 2989 3774
SspI AAT'ATT
Cuts at: 5 52 4687 5
Size: 47 4635 2081
Fragments arranged by size:
4635 2081 47
StuI AGG'CCT
Cuts at: 1517 2282 3113 1517
Size: 765 831 5167
Fragments arranged by size:
5167 831 765
StyI C'CwwG_G
Cuts at: 513 1065 2004 2192 2234 2496 2737 4202 513
Size: 552 939 188 42 262 241 1465 3074
Fragments arranged by size:
3074 1465 939 552 262 241 188 42
SwaI ATTT'AAAT
Cuts at: 3412 3412
Size: 6763
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4790 4807 4960 5145 5590 325
Size: 1255 3210 17 153 185 445 1498
Fragments arranged by size:
3210 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3341 5009 6721
Size: 274 80 33 51 1443 1364 1668 1712
Cuts at: 6721 96
Size: 138
Fragments arranged by size:
1712 1668 1443 1364 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2045 2077 6723 6755 2045
Size: 32 4646 32 2053
Fragments arranged by size:
4646 2053 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6763
XbaI T'CTAG_A
Cuts at: 4099 4099
Size: 6763
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6763
XmaI C'CCGG_G
Cuts at: 1870 2420 4088 1870
Size: 550 1668 4545
Fragments arranged by size:
4545 1668 550
XmnI GAAnn'nnTTC
Cuts at: 2777 3029 3113 3686 4892 2777
Size: 252 84 573 1206 4648
Fragments arranged by size:
4648 1206 573 252 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4571 276
Size: 53 83 186 936 3037 2468
Fragments arranged by size:
3037 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BstZ17I Bsu36I EcoNI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI