Restriction map for
pFN21AE1174
(Circular) (Six-base) MAPSORT of: pFN21AE1174.seq Check: 6264 from: 1 to: 6610
pFN21AE1174 6610 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2470 3062 3223 2470
Size: 592 161 5857
Fragments arranged by size:
5857 592 161
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4420 278
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3953 1937
Size: 2016 4594
Acc65I G'GTAC_C
Cuts at: 3931 3931
Size: 6610
AclI AA'CG_TT
Cuts at: 1563 4737 5110 1563
Size: 3174 373 3063
Fragments arranged by size:
3174 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3407 4673 6057 6325 1632
Size: 1775 1266 1384 268 1917
Fragments arranged by size:
1917 1775 1384 1266 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6413
AflIII A'CryG_T
Cuts at: 1203 5509 6486 1203
Size: 4306 977 1327
Fragments arranged by size:
4306 1327 977
AgeI A'CCGG_T
Cuts at: 2961 5441 2961
Size: 2480 4130
AhdI GACnn_n'nnGTC
Cuts at: 5339 5339
Size: 6610
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6610
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6610
AlwNI CAG_nnn'CTG
Cuts at: 2080 2191 4353 5925 2080
Size: 111 2162 1572 2765
Fragments arranged by size:
2765 2162 1572 111
ApaI G_GGCC'C
Cuts at: 2199 2272 2360 2917 3162 3453 3820 2199
Size: 73 88 557 245 291 367 4989
Fragments arranged by size:
4989 557 367 291 245 88 73
ApaLI G'TGCA_C
Cuts at: 4667 5823 4667
Size: 1156 5454
ApoI r'AATT_y
Cuts at: 1493 3919 4145 4297 6406 1493
Size: 2426 226 152 2109 1697
Fragments arranged by size:
2426 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5164 160
Size: 872 4132 1606
Fragments arranged by size:
4132 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
AvaI C'yCGr_G
Cuts at: 1870 1943 2288 2819 2982 2988 3090 3155 3278
Size: 73 345 531 163 6 102 65 123
Cuts at: 3278 3851 3935 1870
Size: 573 84 4545
Fragments arranged by size:
4545 573 531 345 163 123 102 84
73 65 6
BamHI G'GATC_C
Cuts at: 3940 4307 3940
Size: 367 6243
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2339 2482 2501 2564 2907
Size: 325 217 627 552 143 19 63 343
Cuts at: 2907 3798 3861 3931 5386 618
Size: 891 63 70 1455 1842
Fragments arranged by size:
1842 1455 891 627 552 343 325 217
143 70 63 63 19
BanII G_rGCy'C
Cuts at: 1391 2199 2272 2360 2917 2989 3162 3453 3731
Size: 808 73 88 557 72 173 291 278
Cuts at: 3731 3820 3929 1391
Size: 89 109 4072
Fragments arranged by size:
4072 808 557 291 278 173 109 89
88 73 72
BbeI G_GCGC'C
Cuts at: 2505 2568 3802 2505
Size: 63 1234 5313
Fragments arranged by size:
5313 1234 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3592 928
Size: 971 1693 3946
Fragments arranged by size:
3946 1693 971
BbvCI CC'TCA_GC
Cuts at: 2384 2384
Size: 6610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4801 4835 1903
Size: 34 2864 34 3678
Fragments arranged by size:
3678 2864 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4502 5712 4502
Size: 1210 5400
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2355 2627 2796
Size: 107 122 71 631 605 683 272 169
Cuts at: 2796 3533 5221 136
Size: 737 1688 1525
Fragments arranged by size:
1688 1525 737 683 631 605 272 169
122 107 71
BglII A'GATC_T
Cuts at: 6605 6605
Size: 6610
BlpI GC'TnA_GC
Cuts at: 2093 2690 3662 4027 2093
Size: 597 972 365 4676
Fragments arranged by size:
4676 972 597 365
Bme1580I G_kGCm'C
Cuts at: 1790 2110 2199 2272 2342 2360 2487 2820 2917
Size: 320 89 73 70 18 127 333 97
Cuts at: 2917 3162 3453 3490 3820 4671 5827 1790
Size: 245 291 37 330 851 1156 2573
Fragments arranged by size:
2573 1156 851 333 330 320 291 245
127 97 89 73 70 37 18
BmgBI CAC'GTC
Cuts at: 1318 3670 1318
Size: 2352 4258
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2953 3290 4341
Size: 416 53 83 399 24 1536 337 1051
Cuts at: 4341 5299 442
Size: 958 1753
Fragments arranged by size:
1753 1536 1051 958 416 399 337 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2465 3233 3352 5270 1239
Size: 1226 768 119 1918 2579
Fragments arranged by size:
2579 1918 1226 768 119
Bpu10I CC'TnA_GC
Cuts at: 2384 2384
Size: 6610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3641 4735 5600 5898 3641
Size: 1094 865 298 4353
Fragments arranged by size:
4353 1094 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3332 5273 882
Size: 617 1833 1941 2219
Fragments arranged by size:
2219 1941 1833 617
BsaAI yAC'GTr
Cuts at: 493 1204 3541 4401 493
Size: 711 2337 860 2702
Fragments arranged by size:
2702 2337 860 711
BsaBI GATnn'nnATC
Cuts at: 6604 6604
Size: 6610
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2502 2565 3799 4417
Size: 53 83 186 936 969 63 1234 618
Cuts at: 4417 4799 5434 275
Size: 382 635 1451
Fragments arranged by size:
1451 1234 969 936 635 618 382 186
83 63 53
BsaWI w'CCGG_w
Cuts at: 2632 2961 3351 5043 5441 5715 5862 6273 2632
Size: 329 390 1692 398 274 147 411 2969
Fragments arranged by size:
2969 1692 411 398 390 329 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2591 2614 2621 2644 1178
Size: 30 165 30 1188 23 7 23 5144
Fragments arranged by size:
5144 1188 165 30 30 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2968 3822 1186
Size: 1782 854 3974
Fragments arranged by size:
3974 1782 854
BseYI C'CCAG_C
Cuts at: 2335 2398 2574 3032 3041 3270 3361 3446 3478
Size: 63 176 458 9 229 91 85 32
Cuts at: 3478 3544 3695 5813 6309 2335
Size: 66 151 2118 496 2636
Fragments arranged by size:
2636 2118 496 458 229 176 151 91
85 66 63 32 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2501 2696 3251 1163
Size: 573 765 195 555 4522
Fragments arranged by size:
4522 765 573 555 195
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 2756 2800 2962 3444 3743
Size: 275 186 48 707 44 162 482 299
Cuts at: 3743 4060 4821 5849 1540
Size: 317 761 1028 2301
Fragments arranged by size:
2301 1028 761 707 482 317 299 275
186 162 48 44
BsiHKAI G_wGCw'C
Cuts at: 4671 4756 5827 4671
Size: 85 1071 5454
Fragments arranged by size:
5454 1071 85
BsmI GAATG_Cn'
Cuts at: 4121 4214 4121
Size: 93 6517
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2199 2272 2342 2360 2487 2820
Size: 399 320 89 73 70 18 127 333
Cuts at: 2820 2917 2989 3162 3453 3490 3731 3820 3929
Size: 97 72 173 291 37 241 89 109
Cuts at: 3929 4671 4756 5827 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 333 320 291 241
173 127 109 97 89 89 85 73
72 70 37 18
BspEI T'CCGG_A
Cuts at: 2632 6273 2632
Size: 3641 2969
BspHI T'CATG_A
Cuts at: 4499 6229 4499
Size: 1730 4880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2470 3062 3223 3965 844
Size: 1626 592 161 742 3489
Fragments arranged by size:
3489 1626 742 592 161
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 2045 3182 3889 4497 1394
Size: 429 194 28 1137 707 608 3507
Fragments arranged by size:
3507 1137 707 608 429 194 28
BsrDI GCAATG_nn'
Cuts at: 65 1248 2254 2850 5105 5279 65
Size: 1183 1006 596 2255 174 1396
Fragments arranged by size:
2255 1396 1183 1006 596 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2145 2492 2795 2961 3026 3439 3505
Size: 110 335 347 303 166 65 413 66
Cuts at: 3505 5254 5441 1700
Size: 1749 187 2869
Fragments arranged by size:
2869 1749 413 347 335 303 187 166
110 66 65
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6610
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6610
BssSI C'ACGA_G
Cuts at: 2649 4670 5682 2649
Size: 2021 1012 3577
Fragments arranged by size:
3577 2021 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6610
BstBI TT'CG_AA
Cuts at: 5414 5414
Size: 6610
BstEII G'GTnAC_C
Cuts at: 1184 2921 3022 3244 1184
Size: 1737 101 222 4550
Fragments arranged by size:
4550 1737 222 101
BstXI CCAn_nnnn'nTGG
Cuts at: 2617 3478 3686 3802 2617
Size: 861 208 116 5425
Fragments arranged by size:
5425 861 208 116
BstYI r'GATC_y
Cuts at: 1972 3940 4307 4692 4709 6161 6247 6259 6605
Size: 1968 367 385 17 1452 86 12 346
Cuts at: 6605 1972
Size: 1977
Fragments arranged by size:
1977 1968 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3256 3256
Size: 6610
BtgI C'CryG_G
Cuts at: 513 1065 2004 3121 3561 3567 3722 513
Size: 552 939 1117 440 6 155 3401
Fragments arranged by size:
3401 1117 939 552 440 155 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2392 3043 3154 487
Size: 683 56 1166 651 111 3943
Fragments arranged by size:
3943 1166 683 651 111 56
BtsI GCAGTG_nn'
Cuts at: 2552 2713 2820 3142 3186 4126 4920 4940 2552
Size: 161 107 322 44 940 794 20 4222
Fragments arranged by size:
4222 940 794 322 161 107 44 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6575
DraI TTT'AAA
Cuts at: 3914 3914
Size: 6610
DraIII CAC_nnn'GTG
Cuts at: 3835 3835
Size: 6610
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3214 5617 809
Size: 507 1898 2403 1802
Fragments arranged by size:
2403 1898 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2046 2619
Size: 54 1425 50 275 18 204 12 573
Cuts at: 2619 2753 2793 2797 3284 3441 3465 3564 3643
Size: 134 40 4 487 157 24 99 79
Cuts at: 3643 3740 4057 4946 6575 8
Size: 97 317 889 1629 43
Fragments arranged by size:
1629 1425 889 573 487 317 275 204
157 134 99 97 79 54 50 43
40 24 18 12 4
EagI C'GGCC_G
Cuts at: 1537 1812 2046 2753 2797 3441 3740 4057 1537
Size: 275 234 707 44 644 299 317 4090
Fragments arranged by size:
4090 707 644 317 299 275 234 44
EarI CTCTTCn'nnn_
Cuts at: 1353 3891 4540 1353
Size: 2538 649 3423
Fragments arranged by size:
3423 2538 649
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5197 5571 5717 387
Size: 1064 138 3608 374 146 1280
Fragments arranged by size:
3608 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2465 3233 3352 3407 4673 5270 6057
Size: 393 833 768 119 55 1266 597 787
Cuts at: 6057 6325 1239
Size: 268 1524
Fragments arranged by size:
1524 1266 833 787 768 597 393 268
119 55
EcoNI CCTnn'n_nnAGG
Cuts at: 2477 2477
Size: 6610
EcoO109I rG'GnC_Cy
Cuts at: 2188 2195 2196 2268 2284 2356 2357 2449 2914
Size: 7 1 72 16 72 1 92 465
Cuts at: 2914 3006 3159 3275 3450 3552 3692 2188
Size: 92 153 116 175 102 140 5106
Fragments arranged by size:
5106 465 175 153 140 116 102 92
92 72 72 16 7 1 1
EcoRI G'AATT_C
Cuts at: 3919 4297 3919
Size: 378 6232
FseI GG_CCGG'CC
Cuts at: 2799 2799
Size: 6610
FspI TGC'GCA
Cuts at: 5116 5116
Size: 6610
HaeII r_GCGC'y
Cuts at: 1678 2505 2568 2872 3033 3408 3802 3885 5757
Size: 827 63 304 161 375 394 83 1872
Cuts at: 5757 1678
Size: 2531
Fragments arranged by size:
2531 1872 827 394 375 304 161 83
63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2227 2259 2591 2623 3259
Size: 32 135 32 655 32 332 32 636
Cuts at: 3259 3291 5251 5283 5325 5357 1373
Size: 32 1960 32 42 32 2626
Fragments arranged by size:
2626 1960 655 636 332 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3954 4200 669
Size: 988 281 2016 246 3079
Fragments arranged by size:
3079 2016 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6610
HpaI GTT'AAC
Cuts at: 4200 4200
Size: 6610
KasI G'GCGC_C
Cuts at: 2501 2564 3798 2501
Size: 63 1234 5313
Fragments arranged by size:
5313 1234 63
KpnI G_GTAC'C
Cuts at: 3935 3935
Size: 6610
MfeI C'AATT_G
Cuts at: 4209 4209
Size: 6610
MluI A'CGCG_T
Cuts at: 6486 6486
Size: 6610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2634 5724 5908 1305
Size: 1329 3090 184 2007
Fragments arranged by size:
3090 2007 1329 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 2621 10
Size: 54 1425 343 204 585 3999
Fragments arranged by size:
3999 1425 585 343 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4568 4927 5086 518
Size: 648 3402 359 159 2042
Fragments arranged by size:
3402 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2388 2398 2893 3446 3724 3753 4026
Size: 453 329 10 495 553 278 29 273
Cuts at: 4026 4703 5851 6096 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 553 495 453 329 278
273 245 29 10
NaeI GCC'GGC
Cuts at: 2494 2797 3507 2494
Size: 303 710 5597
Fragments arranged by size:
5597 710 303
NarI GG'CG_CC
Cuts at: 2502 2565 3799 2502
Size: 63 1234 5313
Fragments arranged by size:
5313 1234 63
NcoI C'CATG_G
Cuts at: 513 1065 2004 3121 513
Size: 552 939 1117 4002
Fragments arranged by size:
4002 1117 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6610
NgoMIV G'CCGG_C
Cuts at: 2492 2795 3505 2492
Size: 303 710 5597
Fragments arranged by size:
5597 710 303
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6610
NotI GC'GGCC_GC
Cuts at: 2046 4057 2046
Size: 2011 4599
NspI r_CATG'y
Cuts at: 1132 1229 3968 5513 1132
Size: 97 2739 1545 2229
Fragments arranged by size:
2739 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2191 2487 2917 1348
Size: 843 296 430 5041
Fragments arranged by size:
5041 843 430 296
PciI A'CATG_T
Cuts at: 5509 5509
Size: 6610
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2191 2943 3544 1108
Size: 1083 752 601 4174
Fragments arranged by size:
4174 1083 752 601
PmeI GTTT'AAAC
Cuts at: 3914 3914
Size: 6610
PmlI CAC'GTG
Cuts at: 3541 3541
Size: 6610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2284 2316 2813 2845 4679 4711 6192 6224 2284
Size: 32 497 32 1834 32 1481 32 2670
Fragments arranged by size:
2670 1834 1481 497 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2284 2449 3006 3275 3552 2284
Size: 165 557 269 277 5342
Fragments arranged by size:
5342 557 277 269 165
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6610
PsiI TTA'TAA
Cuts at: 4180 4180
Size: 6610
PspOMI G'GGCC_C
Cuts at: 2195 2268 2356 2913 3158 3449 3816 2195
Size: 73 88 557 245 291 367 4989
Fragments arranged by size:
4989 557 367 291 245 88 73
PstI C_TGCA'G
Cuts at: 830 2373 3534 3962 830
Size: 1543 1161 428 3478
Fragments arranged by size:
3478 1543 1161 428
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6610
PvuII CAG'CTG
Cuts at: 2398 3753 2398
Size: 1355 5255
SacII CC_GC'GG
Cuts at: 3725 3725
Size: 6610
SalI G'TCGA_C
Cuts at: 1936 3952 1936
Size: 2016 4594
SanDI GG'GwC_CC
Cuts at: 3275 3552 3275
Size: 277 6333
SbfI CC_TGCA'GG
Cuts at: 3534 3962 3534
Size: 428 6182
ScaI AGT'ACT
Cuts at: 4858 6570 4858
Size: 1712 4898
SfcI C'TryA_G
Cuts at: 826 1045 2369 3530 3958 5093 5774 5965 826
Size: 219 1324 1161 428 1135 681 191 1471
Fragments arranged by size:
1471 1324 1161 1135 681 428 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2796 2796
Size: 6610
SfoI GGC'GCC
Cuts at: 2503 2566 3800 2503
Size: 63 1234 5313
Fragments arranged by size:
5313 1234 63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6610
SmaI CCC'GGG
Cuts at: 1872 2990 3157 3280 3937 1872
Size: 1118 167 123 657 4545
Fragments arranged by size:
4545 1118 657 167 123
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2288 3656 4714 5615 5877 820
Size: 197 926 345 1368 1058 901 262 1553
Fragments arranged by size:
1553 1368 1058 926 901 345 262 197
SnaBI TAC'GTA
Cuts at: 493 4401 493
Size: 3908 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6610
SphI G_CATG'C
Cuts at: 1132 3968 1132
Size: 2836 3774
SrfI GCCC'GGGC
Cuts at: 2990 2990
Size: 6610
SspI AAT'ATT
Cuts at: 5 52 4534 5
Size: 47 4482 2081
Fragments arranged by size:
4482 2081 47
StuI AGG'CCT
Cuts at: 1517 2118 2669 1517
Size: 601 551 5458
Fragments arranged by size:
5458 601 551
StyI C'CwwG_G
Cuts at: 513 1065 2004 3121 3708 4049 513
Size: 552 939 1117 587 341 3074
Fragments arranged by size:
3074 1117 939 587 552 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2570 2866 2909 2939 3721 4637 4654
Size: 1255 990 296 43 30 782 916 17
Cuts at: 4654 4807 4992 5437 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 990 916 782 445 296 185
153 43 30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3318 4856 6568
Size: 274 80 33 51 1443 1341 1538 1712
Cuts at: 6568 96
Size: 138
Fragments arranged by size:
1712 1538 1443 1341 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2614 2646 3121 3153 3659 3691 6570 6602 2614
Size: 32 475 32 506 32 2879 32 2622
Fragments arranged by size:
2879 2622 506 475 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2239 1316
Size: 923 5687
XbaI T'CTAG_A
Cuts at: 3946 3946
Size: 6610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3259 3475 3836 3259
Size: 216 361 6033
Fragments arranged by size:
6033 361 216
XhoI C'TCGA_G
Cuts at: 1943 2288 1943
Size: 345 6265
XmaI C'CCGG_G
Cuts at: 1870 2988 3155 3278 3935 1870
Size: 1118 167 123 657 4545
Fragments arranged by size:
4545 1118 657 167 123
XmnI GAAnn'nnTTC
Cuts at: 4739 4739
Size: 6610
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4418 276
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoRV FalI FspAI NruI NsiI
PacI PfoI PspXI PsrI RsrII SacI SapI SexAI
SwaI