Restriction map for
pFN21AE1165
(Circular) (Six-base) MAPSORT of: pFN21AE1165.seq Check: 7365 from: 1 to: 6487
pFN21AE1165 6487 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4297 278
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3830 1937
Size: 1893 4594
Acc65I G'GTAC_C
Cuts at: 3808 3808
Size: 6487
AclI AA'CG_TT
Cuts at: 1563 4614 4987 1563
Size: 3051 373 3063
Fragments arranged by size:
3063 3051 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 2608 3198 3744 4550 5934 6202 1632
Size: 947 29 590 546 806 1384 268 1917
Fragments arranged by size:
1917 1384 947 806 590 546 268 29
AfeI AGC'GCT
Cuts at: 3214 3214
Size: 6487
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6290
AflIII A'CryG_T
Cuts at: 1203 5386 6363 1203
Size: 4183 977 1327
Fragments arranged by size:
4183 1327 977
AgeI A'CCGG_T
Cuts at: 5318 5318
Size: 6487
AhdI GACnn_n'nnGTC
Cuts at: 5216 5216
Size: 6487
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6487
AlwNI CAG_nnn'CTG
Cuts at: 2060 4230 5802 2060
Size: 2170 1572 2745
Fragments arranged by size:
2745 2170 1572
ApaI G_GGCC'C
Cuts at: 2427 2427
Size: 6487
ApaLI G'TGCA_C
Cuts at: 4544 5700 4544
Size: 1156 5331
ApoI r'AATT_y
Cuts at: 1493 2410 2775 3248 3796 4022 4174 6283 1493
Size: 917 365 473 548 226 152 2109 1697
Fragments arranged by size:
2109 1697 917 548 473 365 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5041 160
Size: 872 4009 1606
Fragments arranged by size:
4009 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
AvaI C'yCGr_G
Cuts at: 1870 1943 3553 3581 3812 1870
Size: 73 1610 28 231 4545
Fragments arranged by size:
4545 1610 231 73 28
BamHI G'GATC_C
Cuts at: 3817 4184 3817
Size: 367 6120
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 3808 5263 618
Size: 325 217 627 335 1686 1455 1842
Fragments arranged by size:
1842 1686 1455 627 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2427 3806 1391
Size: 1036 1379 4072
Fragments arranged by size:
4072 1379 1036
BbeI G_GCGC'C
Cuts at: 2126 2126
Size: 6487
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2614 3747 928
Size: 971 715 1133 3668
Fragments arranged by size:
3668 1133 971 715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2894 2928 4678 4712 1903
Size: 34 957 34 1750 34 3678
Fragments arranged by size:
3678 1750 957 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4379 5589 4379
Size: 1210 5277
BclI T'GATC_A
Cuts at: 1548 2853 1548
Size: 1305 5182
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3062 5098 136
Size: 107 122 71 631 605 1390 2036 1525
Fragments arranged by size:
2036 1525 1390 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6482 6482
Size: 6487
BlpI GC'TnA_GC
Cuts at: 3141 3904 3141
Size: 763 5724
Bme1580I G_kGCm'C
Cuts at: 1790 2427 4548 5704 1790
Size: 637 2121 1156 2573
Fragments arranged by size:
2573 2121 1156 637
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6487
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2538 4218 5176
Size: 416 53 83 399 24 1121 1680 958
Cuts at: 5176 442
Size: 1753
Fragments arranged by size:
1753 1680 1121 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2940 3615 3795 5147 1239
Size: 1701 675 180 1352 2579
Fragments arranged by size:
2579 1701 1352 675 180
Bpu10I CC'TnA_GC
Cuts at: 3409 3409
Size: 6487
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2421 3592 4612 5477 5775 2421
Size: 1171 1020 865 298 3133
Fragments arranged by size:
3133 1171 1020 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5150 882
Size: 617 3651 2219
Fragments arranged by size:
3651 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4278 493
Size: 711 3074 2702
Fragments arranged by size:
3074 2702 711
BsaBI GATnn'nnATC
Cuts at: 6481 6481
Size: 6487
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2123 3744 4294 4676
Size: 53 83 186 936 590 1621 550 382
Cuts at: 4676 5311 275
Size: 635 1451
Fragments arranged by size:
1621 1451 936 635 590 550 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2163 4920 5318 5592 5739 6150 2163
Size: 2757 398 274 147 411 2500
Fragments arranged by size:
2757 2500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6262
Fragments arranged by size:
6262 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2550 3599 3602 1186
Size: 1364 1049 3 4071
Fragments arranged by size:
4071 1364 1049 3
BseYI C'CCAG_C
Cuts at: 3202 3261 5690 6186 3202
Size: 59 2429 496 3503
Fragments arranged by size:
3503 2429 496 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2177 3468 3620 1163
Size: 573 441 1291 152 4030
Fragments arranged by size:
4030 1291 573 441 152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3937 4698 5726 1540
Size: 275 186 1936 761 1028 2301
Fragments arranged by size:
2301 1936 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4548 4633 5704 4548
Size: 85 1071 5331
Fragments arranged by size:
5331 1071 85
BsmI GAATG_Cn'
Cuts at: 3998 4091 3998
Size: 93 6394
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2427 3806 4548 4633 5704 1391
Size: 399 637 1379 742 85 1071 2174
Fragments arranged by size:
2174 1379 1071 742 637 399 85
BspEI T'CCGG_A
Cuts at: 2163 6150 2163
Size: 3987 2500
BspHI T'CATG_A
Cuts at: 4376 6106 4376
Size: 1730 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3842 844
Size: 2998 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4374 1394
Size: 429 2551 3507
Fragments arranged by size:
3507 2551 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3110 4982 5156 65
Size: 1183 1862 1872 174 1396
Fragments arranged by size:
1872 1862 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3083 5131 5318 1700
Size: 110 1273 2048 187 2869
Fragments arranged by size:
2869 2048 1273 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6487
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6487
BssSI C'ACGA_G
Cuts at: 3078 4547 5559 3078
Size: 1469 1012 4006
Fragments arranged by size:
4006 1469 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6487
BstBI TT'CG_AA
Cuts at: 5291 5291
Size: 6487
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6487
BstYI r'GATC_y
Cuts at: 1972 2160 3752 3817 4184 4569 4586 6038 6124
Size: 188 1592 65 367 385 17 1452 86
Cuts at: 6124 6136 6482 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1592 1452 385 367 346 188 86
65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2457 513
Size: 552 939 453 4543
Fragments arranged by size:
4543 939 552 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2452 487
Size: 683 56 1226 4522
Fragments arranged by size:
4522 1226 683 56
BtsI GCAGTG_nn'
Cuts at: 3768 4003 4797 4817 3768
Size: 235 794 20 5438
Fragments arranged by size:
5438 794 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6487
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2364 2399 529
Size: 35 1800 35 4617
Fragments arranged by size:
4617 1800 35 35
DraI TTT'AAA
Cuts at: 3318 3791 3318
Size: 473 6014
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3272 5494 809
Size: 507 1956 2222 1802
Fragments arranged by size:
2222 1956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3393 3934 4823
Size: 54 1425 50 275 18 1563 541 889
Cuts at: 4823 6452 8
Size: 1629 43
Fragments arranged by size:
1629 1563 1425 889 541 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3934 1537
Size: 275 2122 4090
Fragments arranged by size:
4090 2122 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2009 2142 2307 2755 3507 4417 1353
Size: 656 133 165 448 752 910 3423
Fragments arranged by size:
3423 910 752 656 448 165 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5074 5448 5594 387
Size: 1064 138 3485 374 146 1280
Fragments arranged by size:
3485 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2579 2608 2940 3198 3615 3744 3795
Size: 393 947 29 332 258 417 129 51
Cuts at: 3795 4550 5147 5934 6202 1239
Size: 755 597 787 268 1524
Fragments arranged by size:
1524 947 787 755 597 417 393 332
268 258 129 51 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2130 3584 2130
Size: 1454 5033
EcoO109I rG'GnC_Cy
Cuts at: 2423 2424 2423
Size: 1 6486
EcoRI G'AATT_C
Cuts at: 3796 4174 3796
Size: 378 6109
EcoRV GAT'ATC
Cuts at: 2344 2344
Size: 6487
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 6487
FspI TGC'GCA
Cuts at: 4993 4993
Size: 6487
HaeII r_GCGC'y
Cuts at: 1678 2126 3216 5634 1678
Size: 448 1090 2418 2531
Fragments arranged by size:
2531 2418 1090 448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2152 2184 2671 2703 2794
Size: 32 135 32 580 32 487 32 91
Cuts at: 2794 2826 2984 3016 3290 3322 3612 3644 5128
Size: 32 158 32 274 32 290 32 1484
Cuts at: 5128 5160 5202 5234 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1484 580 487 290 274 158 135
91 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2302 3457 3831 4077 669
Size: 988 281 364 1155 374 246 3079
Fragments arranged by size:
3079 1155 988 374 364 281 246
HindIII A'AGCT_T
Cuts at: 748 3023 748
Size: 2275 4212
HpaI GTT'AAC
Cuts at: 2302 4077 2302
Size: 1775 4712
KasI G'GCGC_C
Cuts at: 2122 2122
Size: 6487
KpnI G_GTAC'C
Cuts at: 3812 3812
Size: 6487
MfeI C'AATT_G
Cuts at: 4086 4086
Size: 6487
MluI A'CGCG_T
Cuts at: 6363 6363
Size: 6487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2979 3513 5601 5785 1305
Size: 1674 534 2088 184 2007
Fragments arranged by size:
2088 2007 1674 534 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3395 10
Size: 54 1425 343 1563 3102
Fragments arranged by size:
3102 1563 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2688 4445 4804 4963 518
Size: 648 1522 1757 359 159 2042
Fragments arranged by size:
2042 1757 1522 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2886 3452 3903 4580 5728 5973 1606
Size: 1280 566 451 677 1148 245 2120
Fragments arranged by size:
2120 1280 1148 677 566 451 245
NaeI GCC'GGC
Cuts at: 3085 3085
Size: 6487
NarI GG'CG_CC
Cuts at: 2123 2123
Size: 6487
NcoI C'CATG_G
Cuts at: 513 1065 2004 2457 513
Size: 552 939 453 4543
Fragments arranged by size:
4543 939 552 453
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6487
NgoMIV G'CCGG_C
Cuts at: 3083 3083
Size: 6487
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6487
NotI GC'GGCC_GC
Cuts at: 3934 3934
Size: 6487
NspI r_CATG'y
Cuts at: 1132 1229 3845 5390 1132
Size: 97 2616 1545 2229
Fragments arranged by size:
2616 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6487
PciI A'CATG_T
Cuts at: 5386 5386
Size: 6487
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3481 1108
Size: 2373 4114
PmeI GTTT'AAAC
Cuts at: 3791 3791
Size: 6487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4556 4588 6069 6101 4556
Size: 32 1481 32 4942
Fragments arranged by size:
4942 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6487
PsiI TTA'TAA
Cuts at: 4057 4057
Size: 6487
PspOMI G'GGCC_C
Cuts at: 2423 2423
Size: 6487
PspXI vC'TCGA_Gb
Cuts at: 3553 3581 3553
Size: 28 6459
PstI C_TGCA'G
Cuts at: 830 2106 2907 3839 830
Size: 1276 801 932 3478
Fragments arranged by size:
3478 1276 932 801
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6487
PvuII CAG'CTG
Cuts at: 3452 3452
Size: 6487
SalI G'TCGA_C
Cuts at: 1936 3829 1936
Size: 1893 4594
SbfI CC_TGCA'GG
Cuts at: 3839 3839
Size: 6487
ScaI AGT'ACT
Cuts at: 4735 6447 4735
Size: 1712 4775
SexAI A'CCwGG_T
Cuts at: 3692 3692
Size: 6487
SfcI C'TryA_G
Cuts at: 826 1045 2052 2102 2734 2903 3835 4970 5651
Size: 219 1007 50 632 169 932 1135 681
Cuts at: 5651 5842 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1007 932 681 632 219 191
169 50
SfoI GGC'GCC
Cuts at: 2124 2124
Size: 6487
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6487
SmaI CCC'GGG
Cuts at: 1872 3814 1872
Size: 1942 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2436 3553 3571 3581 4591 5492
Size: 197 926 493 1117 18 10 1010 901
Cuts at: 5492 5754 820
Size: 262 1553
Fragments arranged by size:
1553 1117 1010 926 901 493 262 197
18 10
SnaBI TAC'GTA
Cuts at: 493 4278 493
Size: 3785 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6487
SphI G_CATG'C
Cuts at: 1132 3845 1132
Size: 2713 3774
SspI AAT'ATT
Cuts at: 5 52 4411 5
Size: 47 4359 2081
Fragments arranged by size:
4359 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6487
StyI C'CwwG_G
Cuts at: 513 1065 2004 2457 3234 3523 3926 513
Size: 552 939 453 777 289 403 3074
Fragments arranged by size:
3074 939 777 552 453 403 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2457 4514 4531 4684 4869 5314 325
Size: 1255 877 2057 17 153 185 445 1498
Fragments arranged by size:
2057 1498 1255 877 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4733 6445 96
Size: 274 80 33 51 1443 2756 1712 138
Fragments arranged by size:
2756 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2723 2755 6447 6479 2723
Size: 32 3692 32 2731
Fragments arranged by size:
3692 2731 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3694 1316
Size: 2378 4109
XbaI T'CTAG_A
Cuts at: 3823 3823
Size: 6487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2139 2139
Size: 6487
XhoI C'TCGA_G
Cuts at: 1943 3553 3581 1943
Size: 1610 28 4849
Fragments arranged by size:
4849 1610 28
XmaI C'CCGG_G
Cuts at: 1870 3812 1870
Size: 1942 4545
XmnI GAAnn'nnTTC
Cuts at: 4616 4616
Size: 6487
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4295 276
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI
FspAI NruI NsiI PacI PfoI PmlI PpuMI PsrI
RsrII SacI SacII SanDI SapI SfiI SrfI SwaI