Restriction map for
pFN21AE1162
(Circular) (Six-base) MAPSORT of: pFN21AE1162.seq Check: 2436 from: 1 to: 6406
pFN21AE1162 6406 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3749 1937
Size: 1812 4594
Acc65I G'GTAC_C
Cuts at: 3727 3727
Size: 6406
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2281 2526 2950 3202 3454 3643 4469 5853
Size: 649 245 424 252 252 189 826 1384
Cuts at: 5853 6121 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 826 649 424 268 252 252
245 189
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 3024 3026 5305 6282 1203
Size: 1821 2 2279 977 1327
Fragments arranged by size:
2279 1821 1327 977 2
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 2739 5135 2739
Size: 2396 4010
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6406
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2055 2087 2055
Size: 32 6374
AlwNI CAG_nnn'CTG
Cuts at: 4149 5721 4149
Size: 1572 4834
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 2514 2974 3168 3252 3715 3941 4093 6202
Size: 1021 460 194 84 463 226 152 2109
Cuts at: 6202 1493
Size: 1697
Fragments arranged by size:
2109 1697 1021 463 460 226 194 152
84
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 2157 3731 1870
Size: 73 214 1574 4545
Fragments arranged by size:
4545 1574 214 73
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3727 5182 618
Size: 325 217 627 1940 1455 1842
Fragments arranged by size:
1940 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3725 1391
Size: 2334 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2956 3208 928
Size: 971 1057 252 4126
Fragments arranged by size:
4126 1057 971 252
BbvCI CC'TCA_GC
Cuts at: 2149 2149
Size: 6406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2961 2995 4597 4631 1903
Size: 34 50 34 940 34 1602 34 3678
Fragments arranged by size:
3678 1602 940 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BfrBI ATG'CAT
Cuts at: 2670 2670
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5017 136
Size: 107 122 71 631 605 3345 1525
Fragments arranged by size:
3345 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2176 6401 2176
Size: 4225 2181
BlpI GC'TnA_GC
Cuts at: 3823 3823
Size: 6406
Bme1580I G_kGCm'C
Cuts at: 1790 4467 5623 1790
Size: 2677 1156 2573
Fragments arranged by size:
2677 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3186 4137 5095
Size: 416 53 83 399 24 1769 951 958
Cuts at: 5095 442
Size: 1753
Fragments arranged by size:
1769 1753 958 951 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2778 2862 2946 3282 3366 3450 3534
Size: 863 676 84 84 336 84 84 84
Cuts at: 3534 5066 1239
Size: 1532 2579
Fragments arranged by size:
2579 1532 863 676 336 84 84 84
84 84
Bpu10I CC'TnA_GC
Cuts at: 2149 2149
Size: 6406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4531 5396 5694 4531
Size: 865 298 5243
Fragments arranged by size:
5243 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2075 2400 5069 882
Size: 617 576 325 2669 2219
Fragments arranged by size:
2669 2219 617 576 325
BsaAI yAC'GTr
Cuts at: 493 1204 3027 4197 493
Size: 711 1823 1170 2702
Fragments arranged by size:
2702 1823 1170 711
BsaBI GATnn'nnATC
Cuts at: 6400 6400
Size: 6406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4213 4595 5230 275
Size: 53 83 186 936 2680 382 635 1451
Fragments arranged by size:
2680 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4839 5237 5511 5658 6069 4839
Size: 398 274 147 411 5176
Fragments arranged by size:
5176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2055 2085 1178
Size: 30 165 30 652 30 5499
Fragments arranged by size:
5499 652 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6406
BseYI C'CCAG_C
Cuts at: 2165 5609 6105 2165
Size: 3444 496 2466
Fragments arranged by size:
3444 2466 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5833
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3856 4617 5645 1540
Size: 275 186 1855 761 1028 2301
Fragments arranged by size:
2301 1855 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4467 4552 5623 4467
Size: 85 1071 5250
Fragments arranged by size:
5250 1071 85
BsmI GAATG_Cn'
Cuts at: 3394 3478 3917 4010 3394
Size: 84 439 93 5790
Fragments arranged by size:
5790 439 93 84
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3725 4467 4552 5623 1391
Size: 399 1935 742 85 1071 2174
Fragments arranged by size:
2174 1935 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3761 844
Size: 2917 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4293 1394
Size: 429 2470 3507
Fragments arranged by size:
3507 2470 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2095 3121 3289 4901 5075 65
Size: 1183 847 1026 168 1612 174 1396
Fragments arranged by size:
1612 1396 1183 1026 847 174 168
BsrFI r'CCGG_y
Cuts at: 1700 1810 5050 5237 1700
Size: 110 3240 187 2869
Fragments arranged by size:
3240 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2895 794
Size: 2101 4305
BstBI TT'CG_AA
Cuts at: 3398 5210 3398
Size: 1812 4594
BstEII G'GTnAC_C
Cuts at: 1184 2225 1184
Size: 1041 5365
BstYI r'GATC_y
Cuts at: 1972 2176 3736 4103 4488 4505 5957 6043 6055
Size: 204 1560 367 385 17 1452 86 12
Cuts at: 6055 6401 1972
Size: 346 1977
Fragments arranged by size:
1977 1560 1452 385 367 346 204 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2551 513
Size: 552 1486 4368
Fragments arranged by size:
4368 1486 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 2107 3922 4716 4736 2107
Size: 1815 794 20 3777
Fragments arranged by size:
3777 1815 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3103 3138 3271 3306 529
Size: 35 2539 35 133 35 3629
Fragments arranged by size:
3629 2539 133 35 35 35
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DraIII CAC_nnn'GTG
Cuts at: 2251 2251
Size: 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3853 4742 6371
Size: 54 1425 50 275 18 2023 889 1629
Cuts at: 6371 8
Size: 43
Fragments arranged by size:
2023 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3853 1537
Size: 275 2041 4090
Fragments arranged by size:
4090 2041 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2280 2416 3662 4336 1353
Size: 927 136 1246 674 3423
Fragments arranged by size:
3423 1246 927 674 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2281 2526 2778 2862 2946 2950
Size: 393 470 179 245 252 84 84 4
Cuts at: 2950 3202 3282 3366 3450 3454 3534 3643 4469
Size: 252 80 84 84 4 80 109 826
Cuts at: 4469 5066 5853 6121 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 826 787 597 470 393 268 252
252 245 179 109 84 84 84 84
80 80 4 4
EcoRI G'AATT_C
Cuts at: 3168 3252 3715 4093 3168
Size: 84 463 378 5481
Fragments arranged by size:
5481 463 378 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2408 2408
Size: 6406
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 5553 1678
Size: 3875 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2193 2725 2757 5047
Size: 32 135 32 589 32 532 32 2290
Cuts at: 5047 5079 5121 5153 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2290 589 532 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 3063 3750 3996 669
Size: 988 281 610 515 687 246 3079
Fragments arranged by size:
3079 988 687 610 515 281 246
HindIII A'AGCT_T
Cuts at: 748 2464 748
Size: 1716 4690
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KpnI G_GTAC'C
Cuts at: 3731 3731
Size: 6406
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2029 5520 5704 1305
Size: 724 3491 184 2007
Fragments arranged by size:
3491 2007 724 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4584
Fragments arranged by size:
4584 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2481 2857 3193 4364 4723 4882 518
Size: 648 1315 376 336 1171 359 159 2042
Fragments arranged by size:
2042 1315 1171 648 376 359 336 159
MspA1I CmG'CkG
Cuts at: 1606 3822 4499 5647 5892 1606
Size: 2216 677 1148 245 2120
Fragments arranged by size:
2216 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2551 513
Size: 552 1486 4368
Fragments arranged by size:
4368 1486 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NsiI A_TGCA'T
Cuts at: 2672 2672
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3764 5309 1132
Size: 97 2535 1545 2229
Fragments arranged by size:
2535 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2075 1348
Size: 727 5679
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6406
PfoI T'CCnGG_A
Cuts at: 2124 2124
Size: 6406
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PmlI CAC'GTG
Cuts at: 3027 3027
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2055 2087 4475 4507 5988 6020 2055
Size: 32 2388 32 1481 32 2441
Fragments arranged by size:
2441 2388 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 3696 3976 3696
Size: 280 6126
PstI C_TGCA'G
Cuts at: 830 2493 3758 830
Size: 1663 1265 3478
Fragments arranged by size:
3478 1663 1265
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SexAI A'CCwGG_T
Cuts at: 2065 2065
Size: 6406
SfcI C'TryA_G
Cuts at: 826 1045 2057 2489 3754 4889 5570 5761 826
Size: 219 1012 432 1265 1135 681 191 1471
Fragments arranged by size:
1471 1265 1135 1012 681 432 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 3733 1872
Size: 1861 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4510 5411 5673 820
Size: 197 926 2567 901 262 1553
Fragments arranged by size:
2567 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6406
StyI C'CwwG_G
Cuts at: 513 1065 2551 3845 513
Size: 552 1486 1294 3074
Fragments arranged by size:
3074 1486 1294 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4433 4450 4603 4788 5233 325
Size: 1255 2853 17 153 185 445 1498
Fragments arranged by size:
2853 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4652 6364 96
Size: 274 80 33 51 1443 2675 1712 138
Fragments arranged by size:
2675 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6366 6398 6366
Size: 32 6374
Tth111I GACn'n_nGTC
Cuts at: 1316 2960 1316
Size: 1644 4762
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 3731 1870
Size: 1861 4545
XmnI GAAnn'nnTTC
Cuts at: 4535 4535
Size: 6406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI