Restriction map for
pFN21AE1157
(Circular) (Six-base) MAPSORT of: pFN21AE1157.seq Check: 3481 from: 1 to: 6400
pFN21AE1157 6400 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4210 278
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3743 1937
Size: 1806 4594
Acc65I G'GTAC_C
Cuts at: 3721 3721
Size: 6400
AclI AA'CG_TT
Cuts at: 1563 3166 4527 4900 1563
Size: 1603 1361 373 3063
Fragments arranged by size:
3063 1603 1361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2004 2299 2430 2682 2919 3083 4463 5847
Size: 372 295 131 252 237 164 1380 1384
Cuts at: 5847 6115 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1380 372 295 268 252 237
164 131
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6203
AflIII A'CryG_T
Cuts at: 1203 3147 5299 6276 1203
Size: 1944 2152 977 1327
Fragments arranged by size:
2152 1944 1327 977
AgeI A'CCGG_T
Cuts at: 5231 5231
Size: 6400
AhdI GACnn_n'nnGTC
Cuts at: 5129 5129
Size: 6400
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6400
AlwNI CAG_nnn'CTG
Cuts at: 2101 2487 2800 4143 5715 2101
Size: 386 313 1343 1572 2786
Fragments arranged by size:
2786 1572 1343 386 313
ApaLI G'TGCA_C
Cuts at: 4457 5613 4457
Size: 1156 5244
ApoI r'AATT_y
Cuts at: 1493 2225 2736 3046 3641 3671 3709 3935 4087
Size: 732 511 310 595 30 38 226 152
Cuts at: 4087 6196 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 732 595 511 310 226 152
38 30
AseI AT'TA_AT
Cuts at: 160 1032 3441 4954 160
Size: 872 2409 1513 1606
Fragments arranged by size:
2409 1606 1513 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
AvaI C'yCGr_G
Cuts at: 1870 1943 3725 1870
Size: 73 1782 4545
Fragments arranged by size:
4545 1782 73
BamHI G'GATC_C
Cuts at: 3730 4097 3730
Size: 367 6033
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3721 5176 618
Size: 325 217 627 1934 1455 1842
Fragments arranged by size:
1934 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2720 3719 1391
Size: 1329 999 4072
Fragments arranged by size:
4072 1329 999
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 2697 3242 928
Size: 971 645 153 545 4086
Fragments arranged by size:
4086 971 645 545 153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4591 4625 1903
Size: 34 2654 34 3678
Fragments arranged by size:
3678 2654 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3481 4292 5502 3481
Size: 811 1210 4379
Fragments arranged by size:
4379 1210 811
BclI T'GATC_A
Cuts at: 1548 2791 1548
Size: 1243 5157
BfrBI ATG'CAT
Cuts at: 2855 2855
Size: 6400
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5011 136
Size: 107 122 71 631 605 3339 1525
Fragments arranged by size:
3339 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2332 6395 2332
Size: 4063 2337
BlpI GC'TnA_GC
Cuts at: 3183 3817 3183
Size: 634 5766
Bme1580I G_kGCm'C
Cuts at: 1790 4461 5617 1790
Size: 2671 1156 2573
Fragments arranged by size:
2671 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2118 2615 1318
Size: 800 497 5103
Fragments arranged by size:
5103 800 497
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2089 4131 5089
Size: 416 53 83 399 24 672 2042 958
Cuts at: 5089 442
Size: 1753
Fragments arranged by size:
2042 1753 958 672 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5060 1239
Size: 3821 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2571 4525 5390 5688 2571
Size: 1954 865 298 3283
Fragments arranged by size:
3283 1954 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3577 5063 882
Size: 617 2078 1486 2219
Fragments arranged by size:
2219 2078 1486 617
BsaAI yAC'GTr
Cuts at: 493 1204 4191 493
Size: 711 2987 2702
Fragments arranged by size:
2987 2702 711
BsaBI GATnn'nnATC
Cuts at: 6394 6394
Size: 6400
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4207 4589 5224 275
Size: 53 83 186 936 2674 382 635 1451
Fragments arranged by size:
2674 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4833 5231 5505 5652 6063 4833
Size: 398 274 147 411 5170
Fragments arranged by size:
5170 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6175
Fragments arranged by size:
6175 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2522 1186
Size: 1336 5064
BseYI C'CCAG_C
Cuts at: 5603 6099 5603
Size: 496 5904
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5827
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3850 4611 5639 1540
Size: 275 186 1849 761 1028 2301
Fragments arranged by size:
2301 1849 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3289 4461 4546 5617 3289
Size: 1172 85 1071 4072
Fragments arranged by size:
4072 1172 1071 85
BsmI GAATG_Cn'
Cuts at: 3137 3911 4004 3137
Size: 774 93 5533
Fragments arranged by size:
5533 774 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2720 3289 3719 4461 4546 5617 1391
Size: 399 930 569 430 742 85 1071 2174
Fragments arranged by size:
2174 1071 930 742 569 430 399 85
BspEI T'CCGG_A
Cuts at: 6063 6063
Size: 6400
BspHI T'CATG_A
Cuts at: 2254 2362 4289 6019 2254
Size: 108 1927 1730 2635
Fragments arranged by size:
2635 1927 1730 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3595 3755 844
Size: 2751 160 3489
Fragments arranged by size:
3489 2751 160
BsrBI CCG'CTC
Cuts at: 1394 1823 4287 1394
Size: 429 2464 3507
Fragments arranged by size:
3507 2464 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3619 4895 5069 65
Size: 1183 2371 1276 174 1396
Fragments arranged by size:
2371 1396 1276 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5044 5231 1700
Size: 110 3234 187 2869
Fragments arranged by size:
3234 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6400
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6400
BssSI C'ACGA_G
Cuts at: 4460 5472 4460
Size: 1012 5388
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6400
BstBI TT'CG_AA
Cuts at: 5204 5204
Size: 6400
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6400
BstYI r'GATC_y
Cuts at: 1972 2332 2903 3730 4097 4482 4499 5951 6037
Size: 360 571 827 367 385 17 1452 86
Cuts at: 6037 6049 6395 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 827 571 385 367 360 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4909
Fragments arranged by size:
4909 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5661
Fragments arranged by size:
5661 683 56
BtsI GCAGTG_nn'
Cuts at: 3357 3916 4710 4730 3357
Size: 559 794 20 5027
Fragments arranged by size:
5027 794 559 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6365
DraI TTT'AAA
Cuts at: 3704 3704
Size: 6400
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5407 809
Size: 507 4091 1802
Fragments arranged by size:
4091 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3847 4736 6365
Size: 54 1425 50 275 18 2017 889 1629
Cuts at: 6365 8
Size: 43
Fragments arranged by size:
2017 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3847 1537
Size: 275 2035 4090
Fragments arranged by size:
4090 2035 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2277 2418 2427 3030 4330 1353
Size: 924 141 9 603 1300 3423
Fragments arranged by size:
3423 1300 924 603 141 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2597 4987 5361 5507 387
Size: 1064 138 1008 2390 374 146 1280
Fragments arranged by size:
2390 1280 1064 1008 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2004 2299 2430 2682 2919 3083 4463
Size: 393 372 295 131 252 237 164 1380
Cuts at: 4463 5060 5847 6115 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1380 787 597 393 372 295 268
252 237 164 131
EcoO109I rG'GnC_Cy
Cuts at: 2806 2806
Size: 6400
EcoRI G'AATT_C
Cuts at: 3709 4087 3709
Size: 378 6022
FspI TGC'GCA
Cuts at: 4906 4906
Size: 6400
HaeII r_GCGC'y
Cuts at: 1678 5547 1678
Size: 3869 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 3002 3034 5041
Size: 32 135 32 980 32 418 32 2007
Cuts at: 5041 5073 5115 5147 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2007 980 418 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3744 3990 669
Size: 988 281 1806 246 3079
Fragments arranged by size:
3079 1806 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6400
HpaI GTT'AAC
Cuts at: 3990 3990
Size: 6400
KpnI G_GTAC'C
Cuts at: 3725 3725
Size: 6400
MfeI C'AATT_G
Cuts at: 3999 3999
Size: 6400
MluI A'CGCG_T
Cuts at: 6276 6276
Size: 6400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5514 5698 1305
Size: 4209 184 2007
Fragments arranged by size:
4209 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4578
Fragments arranged by size:
4578 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2617 3225 3441 4358 4717 4876 518
Size: 648 1451 608 216 917 359 159 2042
Fragments arranged by size:
2042 1451 917 648 608 359 216 159
MspA1I CmG'CkG
Cuts at: 1606 2061 2067 2240 3816 4493 5641 5886 1606
Size: 455 6 173 1576 677 1148 245 2120
Fragments arranged by size:
2120 1576 1148 677 455 245 173 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4909
Fragments arranged by size:
4909 939 552
NdeI CA'TA_TG
Cuts at: 387 3540 387
Size: 3153 3247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6400
NotI GC'GGCC_GC
Cuts at: 3847 3847
Size: 6400
NsiI A_TGCA'T
Cuts at: 2857 2857
Size: 6400
NspI r_CATG'y
Cuts at: 1132 1229 2248 2356 3151 3758 5303 1132
Size: 97 1019 108 795 607 1545 2229
Fragments arranged by size:
2229 1545 1019 795 607 108 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6400
PciI A'CATG_T
Cuts at: 3147 5299 3147
Size: 2152 4248
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2101 3455 1108
Size: 993 1354 4053
Fragments arranged by size:
4053 1354 993
PfoI T'CCnGG_A
Cuts at: 2194 2194
Size: 6400
PmeI GTTT'AAAC
Cuts at: 3704 3704
Size: 6400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4469 4501 5982 6014 4469
Size: 32 1481 32 4855
Fragments arranged by size:
4855 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2806 2806
Size: 6400
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6400
PsiI TTA'TAA
Cuts at: 3970 3970
Size: 6400
PstI C_TGCA'G
Cuts at: 830 3752 830
Size: 2922 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6400
PvuII CAG'CTG
Cuts at: 2240 2240
Size: 6400
SalI G'TCGA_C
Cuts at: 1936 3742 1936
Size: 1806 4594
SanDI GG'GwC_CC
Cuts at: 2806 2806
Size: 6400
SbfI CC_TGCA'GG
Cuts at: 3752 3752
Size: 6400
ScaI AGT'ACT
Cuts at: 2395 2816 4648 6360 2395
Size: 421 1832 1712 2435
Fragments arranged by size:
2435 1832 1712 421
SexAI A'CCwGG_T
Cuts at: 3391 3391
Size: 6400
SfcI C'TryA_G
Cuts at: 826 1045 2488 3748 4883 5564 5755 826
Size: 219 1443 1260 1135 681 191 1471
Fragments arranged by size:
1471 1443 1260 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6400
SmaI CCC'GGG
Cuts at: 1872 3727 1872
Size: 1855 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2586 4504 5405 5667 820
Size: 197 926 643 1918 901 262 1553
Fragments arranged by size:
1918 1553 926 901 643 262 197
SnaBI TAC'GTA
Cuts at: 493 4191 493
Size: 3698 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6400
SphI G_CATG'C
Cuts at: 1132 3758 1132
Size: 2626 3774
SspI AAT'ATT
Cuts at: 5 52 2627 3402 4324 5
Size: 47 2575 775 922 2081
Fragments arranged by size:
2575 2081 922 775 47
StuI AGG'CCT
Cuts at: 1517 3068 1517
Size: 1551 4849
StyI C'CwwG_G
Cuts at: 513 1065 2004 3839 513
Size: 552 939 1835 3074
Fragments arranged by size:
3074 1835 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4427 4444 4597 4782 5227 325
Size: 1255 2847 17 153 185 445 1498
Fragments arranged by size:
2847 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2393 2814 4646
Size: 274 80 33 51 1443 416 421 1832
Cuts at: 4646 6358 96
Size: 1712 138
Fragments arranged by size:
1832 1712 1443 421 416 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6360 6392 6360
Size: 32 6368
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6400
XbaI T'CTAG_A
Cuts at: 2858 3253 3736 2858
Size: 395 483 5522
Fragments arranged by size:
5522 483 395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2691 2691
Size: 6400
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6400
XmaI C'CCGG_G
Cuts at: 1870 3725 1870
Size: 1855 4545
XmnI GAAnn'nnTTC
Cuts at: 2892 4529 2892
Size: 1637 4763
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4208 276
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SfiI SfoI SrfI
SwaI