Restriction map for
pFN21AE1153
(Circular) (Six-base) MAPSORT of: pFN21AE1153.seq Check: 9189 from: 1 to: 6205
pFN21AE1153 6205 bp
With 182 enzymes: SgfI *
October 29, 2012 18:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3548 1937
Size: 1611 4594
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 3504 4332 4705 1563
Size: 1941 828 373 3063
Fragments arranged by size:
3063 1941 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2215 3141 4268 5652 5920 1632
Size: 583 926 1127 1384 268 1917
Fragments arranged by size:
1917 1384 1127 926 583 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 5104 6081 1203
Size: 3901 977 1327
Fragments arranged by size:
3901 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6205
AlwNI CAG_nnn'CTG
Cuts at: 2323 2575 2795 3948 5520 2323
Size: 252 220 1153 1572 3008
Fragments arranged by size:
3008 1572 1153 252 220
ApaI G_GGCC'C
Cuts at: 2083 2117 2083
Size: 34 6171
ApaLI G'TGCA_C
Cuts at: 2895 4262 5418 2895
Size: 1367 1156 3682
Fragments arranged by size:
3682 1367 1156
ApoI r'AATT_y
Cuts at: 1493 2347 2429 2513 2532 3514 3740 3892 6001
Size: 854 82 84 19 982 226 152 2109
Cuts at: 6001 1493
Size: 1697
Fragments arranged by size:
2109 1697 982 854 226 152 84 82
19
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 3530 1870
Size: 73 1587 4545
Fragments arranged by size:
4545 1587 73
BamHI G'GATC_C
Cuts at: 3535 3902 3535
Size: 367 5838
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 3526 4981 618
Size: 325 217 627 304 1435 1455 1842
Fragments arranged by size:
1842 1455 1435 627 325 304 217
BanII G_rGCy'C
Cuts at: 1391 2083 2117 2382 2706 3123 3524 1391
Size: 692 34 265 324 417 401 4072
Fragments arranged by size:
4072 692 417 401 324 265 34
BbeI G_GCGC'C
Cuts at: 2095 2095
Size: 6205
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3324 928
Size: 971 1425 3809
Fragments arranged by size:
3809 1425 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4396 4430 1903
Size: 34 2459 34 3678
Fragments arranged by size:
3678 2459 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4097 5307 4097
Size: 1210 4995
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2729 4816 136
Size: 107 122 71 631 605 1057 2087 1525
Fragments arranged by size:
2087 1525 1057 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6200 6200
Size: 6205
BlpI GC'TnA_GC
Cuts at: 2376 3622 2376
Size: 1246 4959
Bme1580I G_kGCm'C
Cuts at: 1790 2083 2117 2899 4266 5422 1790
Size: 293 34 782 1367 1156 2573
Fragments arranged by size:
2573 1367 1156 782 293 34
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3421 3936 4894
Size: 416 53 83 399 24 2004 515 958
Cuts at: 4894 442
Size: 1753
Fragments arranged by size:
2004 1753 958 515 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3061 3061
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2432 2699 3222 3405 4865 1239
Size: 1193 267 523 183 1460 2579
Fragments arranged by size:
2579 1460 1193 523 267 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2740 2940 4330 5195 5493 2159
Size: 581 200 1390 865 298 2871
Fragments arranged by size:
2871 1390 865 581 298 200
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2642 2986 4868 882
Size: 617 1143 344 1882 2219
Fragments arranged by size:
2219 1882 1143 617 344
BsaAI yAC'GTr
Cuts at: 493 1204 3996 493
Size: 711 2792 2702
Fragments arranged by size:
2792 2702 711
BsaBI GATnn'nnATC
Cuts at: 6199 6199
Size: 6205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2060 2092 2697 4012
Size: 53 83 186 936 527 32 605 1315
Cuts at: 4012 4394 5029 275
Size: 382 635 1451
Fragments arranged by size:
1451 1315 936 635 605 527 382 186
83 53 32
BsaWI w'CCGG_w
Cuts at: 4638 5036 5310 5457 5868 4638
Size: 398 274 147 411 4975
Fragments arranged by size:
4975 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5980
Fragments arranged by size:
5980 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2028 2702 3113 3130 1186
Size: 842 674 411 17 4261
Fragments arranged by size:
4261 842 674 411 17
BseYI C'CCAG_C
Cuts at: 2107 2166 2965 3122 5408 5904 2107
Size: 59 799 157 2286 496 2408
Fragments arranged by size:
2408 2286 799 496 157 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3410 1163
Size: 573 1674 3958
Fragments arranged by size:
3958 1674 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3655 4416 5444 1540
Size: 275 186 1654 761 1028 2301
Fragments arranged by size:
2301 1654 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2175 2289 2382 2899 4266 4351 5422 2175
Size: 114 93 517 1367 85 1071 2958
Fragments arranged by size:
2958 1367 1071 517 114 93 85
BsmI GAATG_Cn'
Cuts at: 3716 3809 3716
Size: 93 6112
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2117 2175 2289 2382 2706 2899
Size: 399 293 34 58 114 93 324 193
Cuts at: 2899 3123 3524 4266 4351 5422 1391
Size: 224 401 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 401 399 324 293 224
193 114 93 85 58 34
BspEI T'CCGG_A
Cuts at: 5868 5868
Size: 6205
BspHI T'CATG_A
Cuts at: 4094 5824 4094
Size: 1730 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3560 844
Size: 2716 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4092 1394
Size: 429 2269 3507
Fragments arranged by size:
3507 2269 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4700 4874 65
Size: 1183 3452 174 1396
Fragments arranged by size:
3452 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2217 4849 5036 1700
Size: 110 407 2632 187 2869
Fragments arranged by size:
2869 2632 407 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6205
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6205
BssSI C'ACGA_G
Cuts at: 2096 2189 4265 5277 2096
Size: 93 2076 1012 3024
Fragments arranged by size:
3024 2076 1012 93
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2260 2665 2918 794
Size: 1466 405 253 4081
Fragments arranged by size:
4081 1466 405 253
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstYI r'GATC_y
Cuts at: 1972 2238 2938 3535 3902 4287 4304 5756 5842
Size: 266 700 597 367 385 17 1452 86
Cuts at: 5842 5854 6200 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 700 597 385 367 346 266
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4714
Fragments arranged by size:
4714 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5466
Fragments arranged by size:
5466 683 56
BtsI GCAGTG_nn'
Cuts at: 2324 2454 3721 4515 4535 2324
Size: 130 1267 794 20 3994
Fragments arranged by size:
3994 1267 794 130 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DraIII CAC_nnn'GTG
Cuts at: 2293 2293
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5212 809
Size: 507 3896 1802
Fragments arranged by size:
3896 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2721 3652 4541
Size: 54 1425 50 275 18 891 931 889
Cuts at: 4541 6170 8
Size: 1629 43
Fragments arranged by size:
1629 1425 931 891 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3652 1537
Size: 275 1840 4090
Fragments arranged by size:
4090 1840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 3273 4135 1353
Size: 792 1128 862 3423
Fragments arranged by size:
3423 1128 862 792
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2135 3493 4792 5166 5312 387
Size: 1064 138 546 1358 1299 374 146 1280
Fragments arranged by size:
1358 1299 1280 1064 546 374 146 138
EcoICRI GAG'CTC
Cuts at: 2380 2380
Size: 6205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2215 2432 2699 3141 3222 3405 4268
Size: 393 583 217 267 442 81 183 863
Cuts at: 4268 4865 5652 5920 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 863 787 597 583 442 393 268
267 217 183 81
EcoO109I rG'GnC_Cy
Cuts at: 2113 2271 2557 2792 2113
Size: 158 286 235 5526
Fragments arranged by size:
5526 286 235 158
EcoRI G'AATT_C
Cuts at: 3514 3892 3514
Size: 378 5827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 6205
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 2095 2198 3164 5352 1678
Size: 417 103 966 2188 2531
Fragments arranged by size:
2531 2188 966 417 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2569 2601 3454 3486 4846
Size: 32 135 32 997 32 853 32 1360
Cuts at: 4846 4878 4920 4952 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1360 997 853 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3445 3549 3795 669
Size: 988 281 1507 104 246 3079
Fragments arranged by size:
3079 1507 988 281 246 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6205
HpaI GTT'AAC
Cuts at: 3445 3795 3445
Size: 350 5855
KasI G'GCGC_C
Cuts at: 2091 2091
Size: 6205
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2867 5319 5503 1305
Size: 1562 2452 184 2007
Fragments arranged by size:
2452 2007 1562 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2723 10
Size: 54 1425 343 891 3492
Fragments arranged by size:
3492 1425 891 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2691 2855 4163 4522 4681 518
Size: 648 1525 164 1308 359 159 2042
Fragments arranged by size:
2042 1525 1308 648 359 164 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2048 2750 3455 3621 4298 5446 5691
Size: 431 11 702 705 166 677 1148 245
Cuts at: 5691 1606
Size: 2120
Fragments arranged by size:
2120 1148 705 702 677 431 245 166
11
NarI GG'CG_CC
Cuts at: 2092 2092
Size: 6205
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4714
Fragments arranged by size:
4714 939 552
NdeI CA'TA_TG
Cuts at: 387 2547 2644 387
Size: 2160 97 3948
Fragments arranged by size:
3948 2160 97
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 3563 5108 1132
Size: 97 2334 1545 2229
Fragments arranged by size:
2334 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6205
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6205
PfoI T'CCnGG_A
Cuts at: 2241 2676 2241
Size: 435 5770
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2557 2557
Size: 6205
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PspOMI G'GGCC_C
Cuts at: 2079 2113 2079
Size: 34 6171
PstI C_TGCA'G
Cuts at: 830 3057 3557 830
Size: 2227 500 3478
Fragments arranged by size:
3478 2227 500
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
PvuII CAG'CTG
Cuts at: 2048 3455 2048
Size: 1407 4798
SacI G_AGCT'C
Cuts at: 2382 2382
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 3115 4453 6165 3115
Size: 1338 1712 3155
Fragments arranged by size:
3155 1712 1338
SfcI C'TryA_G
Cuts at: 826 1045 3053 3357 3553 4688 5369 5560 826
Size: 219 2008 304 196 1135 681 191 1471
Fragments arranged by size:
2008 1471 1135 681 304 219 196 191
SfoI GGC'GCC
Cuts at: 2093 2093
Size: 6205
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 3532 1872
Size: 1660 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2174 2755 2955 4309 5210 5472
Size: 197 926 231 581 200 1354 901 262
Cuts at: 5472 820
Size: 1553
Fragments arranged by size:
1553 1354 926 901 581 262 231 200
197
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3563 1132
Size: 2431 3774
SspI AAT'ATT
Cuts at: 5 52 2596 4129 5
Size: 47 2544 1533 2081
Fragments arranged by size:
2544 2081 1533 47
StuI AGG'CCT
Cuts at: 1517 3132 1517
Size: 1615 4590
StyI C'CwwG_G
Cuts at: 513 1065 2004 2274 2553 3644 513
Size: 552 939 270 279 1091 3074
Fragments arranged by size:
3074 1091 939 552 279 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2158 2824 3262 4232 4249 4402 4587
Size: 1255 578 666 438 970 17 153 185
Cuts at: 4587 5032 325
Size: 445 1498
Fragments arranged by size:
1498 1255 970 666 578 445 438 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2759 3113 3323
Size: 274 80 33 51 1443 782 354 210
Cuts at: 3323 4451 6163 96
Size: 1128 1712 138
Fragments arranged by size:
1712 1443 1128 782 354 274 210 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2279 2311 6165 6197 2279
Size: 32 3854 32 2287
Fragments arranged by size:
3854 2287 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2887 1316
Size: 1571 4634
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2467 3197 2467
Size: 730 5475
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 3530 1870
Size: 1660 4545
XmnI GAAnn'nnTTC
Cuts at: 3291 4334 3291
Size: 1043 5162
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI