Restriction map for
pFN21AE1152
(Circular) (Six-base) MAPSORT of: pFN21AE1152.seq Check: 7024 from: 1 to: 6169
pFN21AE1152 6169 bp
With 182 enzymes: SgfI *
August 16, 2010 14:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3979 278
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3512 1937
Size: 1575 4594
Acc65I G'GTAC_C
Cuts at: 3490 3490
Size: 6169
AclI AA'CG_TT
Cuts at: 1563 4296 4669 1563
Size: 2733 373 3063
Fragments arranged by size:
3063 2733 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2619 3198 4232 5616 5884 1632
Size: 987 579 1034 1384 268 1917
Fragments arranged by size:
1917 1384 1034 987 579 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5972
AflIII A'CryG_T
Cuts at: 1203 2074 2273 5068 6045 1203
Size: 871 199 2795 977 1327
Fragments arranged by size:
2795 1327 977 871 199
AgeI A'CCGG_T
Cuts at: 5000 5000
Size: 6169
AhdI GACnn_n'nnGTC
Cuts at: 4898 4898
Size: 6169
AleI CACnn'nnGTG
Cuts at: 1166 2033 1166
Size: 867 5302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6169
AlwNI CAG_nnn'CTG
Cuts at: 2766 2922 3912 5484 2766
Size: 156 990 1572 3451
Fragments arranged by size:
3451 1572 990 156
ApaLI G'TGCA_C
Cuts at: 4226 5382 4226
Size: 1156 5013
ApoI r'AATT_y
Cuts at: 1493 3478 3704 3856 5965 1493
Size: 1985 226 152 2109 1697
Fragments arranged by size:
2109 1985 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4723 160
Size: 872 3691 1606
Fragments arranged by size:
3691 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
AvaI C'yCGr_G
Cuts at: 1870 1943 2847 3494 1870
Size: 73 904 647 4545
Fragments arranged by size:
4545 904 647 73
BamHI G'GATC_C
Cuts at: 3499 3866 3499
Size: 367 5802
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3490 4945 618
Size: 325 217 627 1703 1455 1842
Fragments arranged by size:
1842 1703 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2515 2738 3488 1391
Size: 1124 223 750 4072
Fragments arranged by size:
4072 1124 750 223
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2421 928
Size: 971 522 4676
Fragments arranged by size:
4676 971 522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4360 4394 1903
Size: 34 2423 34 3678
Fragments arranged by size:
3678 2423 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4061 5271 4061
Size: 1210 4959
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6169
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4780 136
Size: 107 122 71 631 605 3108 1525
Fragments arranged by size:
3108 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2425 6164 2425
Size: 3739 2430
BlpI GC'TnA_GC
Cuts at: 3586 3586
Size: 6169
Bme1580I G_kGCm'C
Cuts at: 1790 2887 4230 5386 1790
Size: 1097 1343 1156 2573
Fragments arranged by size:
2573 1343 1156 1097
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6169
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3900 4858 442
Size: 416 53 83 399 24 2483 958 1753
Fragments arranged by size:
2483 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2076 4829 1239
Size: 837 2753 2579
Fragments arranged by size:
2753 2579 837
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4294 5159 5457 4294
Size: 865 298 5006
Fragments arranged by size:
5006 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4832 882
Size: 617 3333 2219
Fragments arranged by size:
3333 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2284 2779 3960 493
Size: 711 1080 495 1181 2702
Fragments arranged by size:
2702 1181 1080 711 495
BsaBI GATnn'nnATC
Cuts at: 6163 6163
Size: 6169
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3976 4358 4993 275
Size: 53 83 186 936 2443 382 635 1451
Fragments arranged by size:
2443 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2703 3148 4602 5000 5274 5421 5832 2703
Size: 445 1454 398 274 147 411 3040
Fragments arranged by size:
3040 1454 445 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3352 3382 1178
Size: 30 165 30 1949 30 3965
Fragments arranged by size:
3965 1949 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3237 3360 1186
Size: 2051 123 3995
Fragments arranged by size:
3995 2051 123
BseYI C'CCAG_C
Cuts at: 2048 5372 5868 2048
Size: 3324 496 2349
Fragments arranged by size:
3324 2349 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5596
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3619 4380 5408 1540
Size: 275 186 1618 761 1028 2301
Fragments arranged by size:
2301 1618 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2252 3063 4230 4315 5386 2252
Size: 811 1167 85 1071 3035
Fragments arranged by size:
3035 1167 1071 811 85
BsmI GAATG_Cn'
Cuts at: 2185 3680 3773 2185
Size: 1495 93 4581
Fragments arranged by size:
4581 1495 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2252 2515 2738 2887 3063 3488 4230
Size: 399 462 263 223 149 176 425 742
Cuts at: 4230 4315 5386 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 462 425 399 263 223
176 149 85
BspEI T'CCGG_A
Cuts at: 2703 5832 2703
Size: 3129 3040
BspHI T'CATG_A
Cuts at: 4058 5788 4058
Size: 1730 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2140 2188 3524 844
Size: 1296 48 1336 3489
Fragments arranged by size:
3489 1336 1296 48
BsrBI CCG'CTC
Cuts at: 1394 1823 4056 1394
Size: 429 2233 3507
Fragments arranged by size:
3507 2233 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4664 4838 65
Size: 1183 3416 174 1396
Fragments arranged by size:
3416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4813 5000 1700
Size: 110 3003 187 2869
Fragments arranged by size:
3003 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6169
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6169
BssSI C'ACGA_G
Cuts at: 4229 5241 4229
Size: 1012 5157
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6169
BstBI TT'CG_AA
Cuts at: 4973 4973
Size: 6169
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6169
BstXI CCAn_nnnn'nTGG
Cuts at: 3436 3436
Size: 6169
BstYI r'GATC_y
Cuts at: 1972 2425 3499 3866 4251 4268 5720 5806 5818
Size: 453 1074 367 385 17 1452 86 12
Cuts at: 5818 6164 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1074 453 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4678
Fragments arranged by size:
4678 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5430
Fragments arranged by size:
5430 683 56
BtsI GCAGTG_nn'
Cuts at: 3685 4479 4499 3685
Size: 794 20 5355
Fragments arranged by size:
5355 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2923 2958 529
Size: 35 2359 35 3740
Fragments arranged by size:
3740 2359 35 35
DraI TTT'AAA
Cuts at: 2685 3473 2685
Size: 788 5381
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5176 809
Size: 507 3860 1802
Fragments arranged by size:
3860 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2162 3616 4505
Size: 54 1425 50 275 18 332 1454 889
Cuts at: 4505 6134 8
Size: 1629 43
Fragments arranged by size:
1629 1454 1425 889 332 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3616 1537
Size: 275 1804 4090
Fragments arranged by size:
4090 1804 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2295 2826 2850 4099 1353
Size: 942 531 24 1249 3423
Fragments arranged by size:
3423 1249 942 531 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4756 5130 5276 387
Size: 1064 138 3167 374 146 1280
Fragments arranged by size:
3167 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 2619 3198 4232 4829 5616 5884
Size: 393 444 543 579 1034 597 787 268
Cuts at: 5884 1239
Size: 1524
Fragments arranged by size:
1524 1034 787 597 579 543 444 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2195 2195
Size: 6169
EcoO109I rG'GnC_Cy
Cuts at: 2200 2200
Size: 6169
EcoRI G'AATT_C
Cuts at: 3478 3856 3478
Size: 378 5791
FspI TGC'GCA
Cuts at: 4675 4675
Size: 6169
HaeII r_GCGC'y
Cuts at: 1678 5316 1678
Size: 3638 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2571 2603 2733 2765 2936
Size: 32 135 32 999 32 130 32 171
Cuts at: 2936 2968 4810 4842 4884 4916 1373
Size: 32 1842 32 42 32 2626
Fragments arranged by size:
2626 1842 999 171 135 130 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2965 3513 3759 669
Size: 988 281 1027 548 246 3079
Fragments arranged by size:
3079 1027 988 548 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6169
HpaI GTT'AAC
Cuts at: 3759 3759
Size: 6169
KpnI G_GTAC'C
Cuts at: 3494 3494
Size: 6169
MfeI C'AATT_G
Cuts at: 3333 3768 3333
Size: 435 5734
MluI A'CGCG_T
Cuts at: 6045 6045
Size: 6169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2645 5283 5467 1305
Size: 1340 2638 184 2007
Fragments arranged by size:
2638 2007 1340 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2164 10
Size: 54 1425 343 332 4015
Fragments arranged by size:
4015 1425 343 332 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2178 2870 3101 4127 4486 4645
Size: 648 867 145 692 231 1026 359 159
Cuts at: 4645 518
Size: 2042
Fragments arranged by size:
2042 1026 867 692 648 359 231 159
145
MspA1I CmG'CkG
Cuts at: 1606 2987 3585 4262 5410 5655 1606
Size: 1381 598 677 1148 245 2120
Fragments arranged by size:
2120 1381 1148 677 598 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4678
Fragments arranged by size:
4678 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6169
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6169
NotI GC'GGCC_GC
Cuts at: 3616 3616
Size: 6169
NspI r_CATG'y
Cuts at: 1132 1229 2078 2277 2723 3527 5072 1132
Size: 97 849 199 446 804 1545 2229
Fragments arranged by size:
2229 1545 849 804 446 199 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6169
PciI A'CATG_T
Cuts at: 2074 2273 5068 2074
Size: 199 2795 3175
Fragments arranged by size:
3175 2795 199
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2373 2766 2922 1108
Size: 1265 393 156 4355
Fragments arranged by size:
4355 1265 393 156
PfoI T'CCnGG_A
Cuts at: 2433 2464 2433
Size: 31 6138
PmeI GTTT'AAAC
Cuts at: 3473 3473
Size: 6169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4238 4270 5751 5783 4238
Size: 32 1481 32 4624
Fragments arranged by size:
4624 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2200 2200
Size: 6169
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6169
PsiI TTA'TAA
Cuts at: 3739 3739
Size: 6169
PstI C_TGCA'G
Cuts at: 830 2899 3014 3521 830
Size: 2069 115 507 3478
Fragments arranged by size:
3478 2069 507 115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6169
PvuII CAG'CTG
Cuts at: 2987 2987
Size: 6169
SalI G'TCGA_C
Cuts at: 1936 3511 1936
Size: 1575 4594
SbfI CC_TGCA'GG
Cuts at: 3521 3521
Size: 6169
ScaI AGT'ACT
Cuts at: 4417 6129 4417
Size: 1712 4457
SfcI C'TryA_G
Cuts at: 826 1045 2895 3010 3186 3517 4652 5333 5524
Size: 219 1850 115 176 331 1135 681 191
Cuts at: 5524 826
Size: 1471
Fragments arranged by size:
1850 1471 1135 681 331 219 191 176
115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6169
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6169
SmaI CCC'GGG
Cuts at: 1872 2849 3496 1872
Size: 977 647 4545
Fragments arranged by size:
4545 977 647
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4273 5174 5436 820
Size: 197 926 2330 901 262 1553
Fragments arranged by size:
2330 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2284 2779 3960 493
Size: 1791 495 1181 2702
Fragments arranged by size:
2702 1791 1181 495
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6169
SphI G_CATG'C
Cuts at: 1132 3527 1132
Size: 2395 3774
SspI AAT'ATT
Cuts at: 5 52 2396 4093 5
Size: 47 2344 1697 2081
Fragments arranged by size:
2344 2081 1697 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6169
StyI C'CwwG_G
Cuts at: 513 1065 2004 2505 3608 513
Size: 552 939 501 1103 3074
Fragments arranged by size:
3074 1103 939 552 501
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4196 4213 4366 4551 4996 325
Size: 1255 2616 17 153 185 445 1498
Fragments arranged by size:
2616 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2486 4415 6127
Size: 274 80 33 51 1443 509 1929 1712
Cuts at: 6127 96
Size: 138
Fragments arranged by size:
1929 1712 1443 509 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6129 6161 6129
Size: 32 6137
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6169
XbaI T'CTAG_A
Cuts at: 3505 3505
Size: 6169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2172 2172
Size: 6169
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6169
XmaI C'CCGG_G
Cuts at: 1870 2847 3494 1870
Size: 977 647 4545
Fragments arranged by size:
4545 977 647
XmnI GAAnn'nnTTC
Cuts at: 2304 4298 2304
Size: 1994 4175
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3977 276
Size: 53 83 186 936 2443 2468
Fragments arranged by size:
2468 2443 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI